TY  - JOUR
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Skorić, Marijana
AU  - Đuricković, Milutin S
AU  - Nestorović Živković, Jasmina
AU  - Jovanović, Vladimir
AU  - Giba, Zlatko
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/962
AB  - Secoiridoid glycosides are naturally occurring phytochemicals of great importance for the food and pharmaceutical industry because of their various biological activities. Certain Gentiana and Centaurium species., which are recognized as the most important sources of these compounds, have become critically endangered due to overexploitation. In this study we describe a laboratory-scale approach for further implementation in large-scale production of secoiridoid glycosides, using a hairy root culture system of Centaurium maritimum L. Fritch, an underutilized and phytochemically unexplored species. Hairy roots were induced by Agrobacterium rhizogenes strain A40M70GUS and grown in Erlenmeyer flasks, as well as in RITA temporary immersion bioreactors (TIBs). About 2-4 times higher biomass production rate and up to 8 times higher total secoiridoid glycosides production rate were achieved in RITA bioreactors. Among the selected hairy root lines, line HR3 cultured in RITA (R) TIBs proved to be the most efficient considering both biomass and secoiridoid glycosides production rate. (C) 2013 Elsevier Ltd. All rights reserved.
T2  - Process Biochemistry
T1  - Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor
IS  - 10
VL  - 48
SP  - 224
EP  - 1591
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_962
ER  - 

@article{
author = "Mišić, Danijela and Šiler, Branislav and Skorić, Marijana and Đuricković, Milutin S and Nestorović Živković, Jasmina and Jovanović, Vladimir and Giba, Zlatko",
year = "2013",
abstract = "Secoiridoid glycosides are naturally occurring phytochemicals of great importance for the food and pharmaceutical industry because of their various biological activities. Certain Gentiana and Centaurium species., which are recognized as the most important sources of these compounds, have become critically endangered due to overexploitation. In this study we describe a laboratory-scale approach for further implementation in large-scale production of secoiridoid glycosides, using a hairy root culture system of Centaurium maritimum L. Fritch, an underutilized and phytochemically unexplored species. Hairy roots were induced by Agrobacterium rhizogenes strain A40M70GUS and grown in Erlenmeyer flasks, as well as in RITA temporary immersion bioreactors (TIBs). About 2-4 times higher biomass production rate and up to 8 times higher total secoiridoid glycosides production rate were achieved in RITA bioreactors. Among the selected hairy root lines, line HR3 cultured in RITA (R) TIBs proved to be the most efficient considering both biomass and secoiridoid glycosides production rate. (C) 2013 Elsevier Ltd. All rights reserved.",
journal = "Process Biochemistry",
title = "Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor",
number = "10",
volume = "48",
pages = "224-1591",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_962"
}

Mišić, D., Šiler, B., Skorić, M., Đuricković, M. S., Nestorović Živković, J., Jovanović, V.,& Giba, Z.. (2013). Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor. in Process Biochemistry, 48(10), 224-1591.
https://hdl.handle.net/21.15107/rcub_ibiss_962

Mišić D, Šiler B, Skorić M, Đuricković MS, Nestorović Živković J, Jovanović V, Giba Z. Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor. in Process Biochemistry. 2013;48(10):224-1591.
https://hdl.handle.net/21.15107/rcub_ibiss_962 .

Mišić, Danijela, Šiler, Branislav, Skorić, Marijana, Đuricković, Milutin S, Nestorović Živković, Jasmina, Jovanović, Vladimir, Giba, Zlatko, "Secoiridoid glycosides production by Centaurium maritimum (L.) Fritch hairy root cultures in temporary immersion bioreactor" in Process Biochemistry, 48, no. 10 (2013):224-1591,
https://hdl.handle.net/21.15107/rcub_ibiss_962 .

TY  - JOUR
AU  - Mišić, Danijela
AU  - Šiler, Branislav
AU  - Nestorović Živković, Jasmina
AU  - Simonović, Ana
AU  - Maksimović, Vuk M
AU  - Budimir, Snežana
AU  - Janošević, Dušica A.
AU  - Đuricković, Milutin S
AU  - Nikolić, Miroslav
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1204
AB  - The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.
T2  - Plant Cell Tissue and Organ Culture
T1  - Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress
IS  - 3
VL  - 108
EP  - 400
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1204
ER  - 

@article{
author = "Mišić, Danijela and Šiler, Branislav and Nestorović Živković, Jasmina and Simonović, Ana and Maksimović, Vuk M and Budimir, Snežana and Janošević, Dušica A. and Đuricković, Milutin S and Nikolić, Miroslav",
year = "2012",
abstract = "The effect of reduced availability of sugars on growth and essential metabolic processes in roots, resulting from decreased photosynthesis under salinity, was excluded by establishing a non-photosynthetic model-system in this study: root cultures of Centaurium maritimum (L.) Fritch and Centaurium spicatum (L.) Fritch. The contribution of inorganic cations and organic compounds (e. g. carbohydrates and amino acids) to the osmotic adjustment (OA) in roots during short-term exposure to various salt concentrations (0, 50, 100 or 200 mM NaCl) was emphasized. Observed morphological and histological changes in roots were species specific, and were dependent on salinity level. Although C. spicatum appears to be more tolerant to salt stress, both species employed similar strategies in response to elevated salinity to different extents, and displayed effective OA mechanisms. Under low and moderate salinity, inorganic cations were the major contributors to OA in roots of both species, followed by soluble sugars, while the relative contribution of proline (Pro) and free amino acids was insignificant. Osmotic adjustment under severe stress appears to be mediated by increased accumulation of organic compounds. The analysis of the intraspecies variability in salt response of C. spicatum and C. maritimum roots enabled the identification of some organic compounds which could be used as potential biochemical markers in screening for salt tolerance, including Pro in C. spicatum, and trehalose and polyols in C. maritimum.",
journal = "Plant Cell Tissue and Organ Culture",
title = "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress",
number = "3",
volume = "108",
pages = "400",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1204"
}

Mišić, D., Šiler, B., Nestorović Živković, J., Simonović, A., Maksimović, V. M., Budimir, S., Janošević, D. A., Đuricković, M. S.,& Nikolić, M.. (2012). Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture, 108(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1204

Mišić D, Šiler B, Nestorović Živković J, Simonović A, Maksimović VM, Budimir S, Janošević DA, Đuricković MS, Nikolić M. Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress. in Plant Cell Tissue and Organ Culture. 2012;108(3):null-400.
https://hdl.handle.net/21.15107/rcub_ibiss_1204 .

Mišić, Danijela, Šiler, Branislav, Nestorović Živković, Jasmina, Simonović, Ana, Maksimović, Vuk M, Budimir, Snežana, Janošević, Dušica A., Đuricković, Milutin S, Nikolić, Miroslav, "Contribution of inorganic cations and organic compounds to osmotic adjustment in root cultures of two Centaurium species differing in tolerance to salt stress" in Plant Cell Tissue and Organ Culture, 108, no. 3 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1204 .