TY  - JOUR
AU  - Zivić, Miroslav
AU  - Popović, Marko
AU  - Todorović, Nataša
AU  - Vucinić, Zeljko B
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1434
AB  - In the present report, by using a patch clamp technique, we provide, to our knowledge, the first detailed description of an anionic channel from filamentous fungi. The characterized channel, an outwardly rectifying anionic channel (ORAC), is the most prominent feature of the cell membrane of the fungus Phycomyces blakesleeanus in the absence of energizing substrates. The unitary conductance of the channel is 11.3 +/- 0.4 pS. It is characterized by a strong voltage dependence of the open-channel probability (z delta; the gating charge is 2.1 +/- 0.1), and the channel is activated by depolarization. The values of the time constants for voltage-induced activation and deactivation of 28 +/- 3 ms for tau(a) and 39 +/- 9 ms for tau(d) show that the ORAC is characterized by fast activation/deactivation kinetics. The ORAC shows strong selectivity for anions over cations and weak selectivity among anions, with a selectivity sequence of I- >= NO(3)(-) > Br(-) > Cl(-) > SO(4)(2-) = 4.8 > 4.4 > 2.2 > 1 > 0.55, which corresponds to Eisenman series 1. The channel is characterized by two open and two closed states, with dominant long open (tau(o2) = 35.0 +/- 3.9 ms) and long closed (tau(c2) = 166 +/- 28 ms) states occupying 63% +/- 8% and 79% +/- 3% of total open and closed times, respectively. The ORAC is insensitive to anthracene-9-carboxylic acid (< 200 mu M), but 2 mM malate reversibly inhibits 59% +/- 12% of the channel activity. Based on the electrophysiological properties of the channel, we propose that the ORAC plays a role in anion accumulation and in membrane potential regulation through local membrane depolarization.
PB  - Washington: American Society of Microbiology
T2  - Eukaryotic Cell
T1  - Outwardly Rectifying Anionic Channel from the Plasma Membrane of the Fungus Phycomyces blakesleeanus
IS  - 9
VL  - 8
DO  - Outwardly Rectifying Anionic Channel from the Plasma Membrane of the Fungus Phycomyces blakesleeanus
SP  - 1439
EP  - 1448
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1434
ER  - 

@article{
author = "Zivić, Miroslav and Popović, Marko and Todorović, Nataša and Vucinić, Zeljko B",
year = "2009",
abstract = "In the present report, by using a patch clamp technique, we provide, to our knowledge, the first detailed description of an anionic channel from filamentous fungi. The characterized channel, an outwardly rectifying anionic channel (ORAC), is the most prominent feature of the cell membrane of the fungus Phycomyces blakesleeanus in the absence of energizing substrates. The unitary conductance of the channel is 11.3 +/- 0.4 pS. It is characterized by a strong voltage dependence of the open-channel probability (z delta; the gating charge is 2.1 +/- 0.1), and the channel is activated by depolarization. The values of the time constants for voltage-induced activation and deactivation of 28 +/- 3 ms for tau(a) and 39 +/- 9 ms for tau(d) show that the ORAC is characterized by fast activation/deactivation kinetics. The ORAC shows strong selectivity for anions over cations and weak selectivity among anions, with a selectivity sequence of I- >= NO(3)(-) > Br(-) > Cl(-) > SO(4)(2-) = 4.8 > 4.4 > 2.2 > 1 > 0.55, which corresponds to Eisenman series 1. The channel is characterized by two open and two closed states, with dominant long open (tau(o2) = 35.0 +/- 3.9 ms) and long closed (tau(c2) = 166 +/- 28 ms) states occupying 63% +/- 8% and 79% +/- 3% of total open and closed times, respectively. The ORAC is insensitive to anthracene-9-carboxylic acid (< 200 mu M), but 2 mM malate reversibly inhibits 59% +/- 12% of the channel activity. Based on the electrophysiological properties of the channel, we propose that the ORAC plays a role in anion accumulation and in membrane potential regulation through local membrane depolarization.",
publisher = "Washington: American Society of Microbiology",
journal = "Eukaryotic Cell",
title = "Outwardly Rectifying Anionic Channel from the Plasma Membrane of the Fungus Phycomyces blakesleeanus",
number = "9",
volume = "8",
doi = "Outwardly Rectifying Anionic Channel from the Plasma Membrane of the Fungus Phycomyces blakesleeanus",
pages = "1439-1448",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1434"
}

Zivić, M., Popović, M., Todorović, N.,& Vucinić, Z. B.. (2009). Outwardly Rectifying Anionic Channel from the Plasma Membrane of the Fungus Phycomyces blakesleeanus. in Eukaryotic Cell
Washington: American Society of Microbiology., 8(9), 1439-1448.
https://doi.org/Outwardly Rectifying Anionic Channel from the Plasma Membrane of the Fungus Phycomyces blakesleeanus
https://hdl.handle.net/21.15107/rcub_ibiss_1434

Zivić M, Popović M, Todorović N, Vucinić ZB. Outwardly Rectifying Anionic Channel from the Plasma Membrane of the Fungus Phycomyces blakesleeanus. in Eukaryotic Cell. 2009;8(9):1439-1448.
doi:Outwardly Rectifying Anionic Channel from the Plasma Membrane of the Fungus Phycomyces blakesleeanus
https://hdl.handle.net/21.15107/rcub_ibiss_1434 .

Zivić, Miroslav, Popović, Marko, Todorović, Nataša, Vucinić, Zeljko B, "Outwardly Rectifying Anionic Channel from the Plasma Membrane of the Fungus Phycomyces blakesleeanus" in Eukaryotic Cell, 8, no. 9 (2009):1439-1448,
https://doi.org/Outwardly Rectifying Anionic Channel from the Plasma Membrane of the Fungus Phycomyces blakesleeanus .,
https://hdl.handle.net/21.15107/rcub_ibiss_1434 .

TY  - JOUR
AU  - Harhaji-Trajković, Ljubica
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Stojanović, Ivana D.
AU  - Momčilović, Miljana
AU  - Maksimović, Vuk M.
AU  - Tufegdžić, Srđan J.
AU  - Marjanović, Zaklina S.
AU  - Mostarica-Stojković, Marija B.
AU  - Vučinić, Zeljko B.
AU  - Stošić-Grujičić, Stanislava
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1531
AB  - Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity. (c) 2008 Elsevier Ltd. All rights reserved.
PB  - Elsevier
T2  - Food and Chemical Toxicology
T1  - Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study
IS  - 5
VL  - 46
DO  - 10.1016/j.fct.2008.01.027
SP  - 1825
EP  - 1833
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1531
ER  - 

@article{
author = "Harhaji-Trajković, Ljubica and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, Ivana D. and Momčilović, Miljana and Maksimović, Vuk M. and Tufegdžić, Srđan J. and Marjanović, Zaklina S. and Mostarica-Stojković, Marija B. and Vučinić, Zeljko B. and Stošić-Grujičić, Stanislava",
year = "2008",
abstract = "Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity. (c) 2008 Elsevier Ltd. All rights reserved.",
publisher = "Elsevier",
journal = "Food and Chemical Toxicology",
title = "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study",
number = "5",
volume = "46",
doi = "10.1016/j.fct.2008.01.027",
pages = "1825-1833",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1531"
}

Harhaji-Trajković, L., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I. D., Momčilović, M., Maksimović, V. M., Tufegdžić, S. J., Marjanović, Z. S., Mostarica-Stojković, M. B., Vučinić, Z. B.,& Stošić-Grujičić, S.. (2008). Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology
Elsevier., 46(5), 1825-1833.
https://doi.org/10.1016/j.fct.2008.01.027
https://hdl.handle.net/21.15107/rcub_ibiss_1531

Harhaji-Trajković L, Mijatović S, Maksimović-Ivanić D, Stojanović ID, Momčilović M, Maksimović VM, Tufegdžić SJ, Marjanović ZS, Mostarica-Stojković MB, Vučinić ZB, Stošić-Grujičić S. Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology. 2008;46(5):1825-1833.
doi:10.1016/j.fct.2008.01.027
https://hdl.handle.net/21.15107/rcub_ibiss_1531 .

Harhaji-Trajković, Ljubica, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, Ivana D., Momčilović, Miljana, Maksimović, Vuk M., Tufegdžić, Srđan J., Marjanović, Zaklina S., Mostarica-Stojković, Marija B., Vučinić, Zeljko B., Stošić-Grujičić, Stanislava, "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study" in Food and Chemical Toxicology, 46, no. 5 (2008):1825-1833,
https://doi.org/10.1016/j.fct.2008.01.027 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1531 .