TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Arsenović-Ranin, Nevena
AU  - Bufan, Biljana
AU  - Nacka-Aleksić, Mirjana
AU  - Lazarević Macanović, Mirjana
AU  - Milovanović, Petar
AU  - Đurić, Marija
AU  - Sopta, Jelena
AU  - Leposavić, Gordana
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S0014480017305646?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3076
AB  - Collagen-induced arthritis (CIA) is a frequently used animal model of rheumatoid arthritis, human autoimmune disease that exhibits clear sex bias in incidence and clinical course. Female Dark Agouti rats immunized for CIA showed also greater incidence and higher arthritic score than their male counterparts. The study investigated sex differences in mechanisms controlling the primary immune responses in draining lymph nodes (dLNs), as a factor contributing to this dimorphism. The higher frequencies of CD4 + CD25 + Foxp3- cells, presumably activated effector T (Teff) cells, and IL-17+, IFN-γ + and IL-17 + IFN-γ + T cells were found in female compared with male rat dLNs. However, the frequency of CD4 + CD25 + Foxp3+ T regulatory cells (Treg) did not differ between sexes. Thus, CD4+ Teff cells/Treg ratio, and IL-17+ T cells/Treg and IFN-γ + T cells/Treg ratios were higher in female than in male rats, and among them was found lower frequency of PD-1+ cells. This suggested less efficient control of (auto)immune Th1/Th17 cell responses in female rat dLNs. On the contrary, the frequency of IL-4+ T cells was lower in female than in male rat dLNs. Consistently, the ratio of serum levels of collagen-specific IgG2a (IFN-γ-dependent, with an important pathogenic role in CIA) and IgG1 (IL-4-dependent) was shifted towards IgG2a in female compared with male rats. As a whole, the study suggests that sexual dimorphism in the control of T cell activation/polarization could contribute to sex bias in the susceptibility to CIA. Moreover, the study advises the use of animals of both sexes in the preclinical testing of new drugs for rheumatoid arthritis.
T2  - Experimental and Molecular Pathology
T1  - Collagen-induced arthritis in Dark Agouti rats as a model for study of immunological sexual dimorphisms in the human disease.
IS  - 1
VL  - 105
DO  - 10.1016/j.yexmp.2018.05.007
SP  - 10
EP  - 22
ER  - 

@article{
author = "Dimitrijević, Mirjana and Arsenović-Ranin, Nevena and Bufan, Biljana and Nacka-Aleksić, Mirjana and Lazarević Macanović, Mirjana and Milovanović, Petar and Đurić, Marija and Sopta, Jelena and Leposavić, Gordana",
year = "2018",
abstract = "Collagen-induced arthritis (CIA) is a frequently used animal model of rheumatoid arthritis, human autoimmune disease that exhibits clear sex bias in incidence and clinical course. Female Dark Agouti rats immunized for CIA showed also greater incidence and higher arthritic score than their male counterparts. The study investigated sex differences in mechanisms controlling the primary immune responses in draining lymph nodes (dLNs), as a factor contributing to this dimorphism. The higher frequencies of CD4 + CD25 + Foxp3- cells, presumably activated effector T (Teff) cells, and IL-17+, IFN-γ + and IL-17 + IFN-γ + T cells were found in female compared with male rat dLNs. However, the frequency of CD4 + CD25 + Foxp3+ T regulatory cells (Treg) did not differ between sexes. Thus, CD4+ Teff cells/Treg ratio, and IL-17+ T cells/Treg and IFN-γ + T cells/Treg ratios were higher in female than in male rats, and among them was found lower frequency of PD-1+ cells. This suggested less efficient control of (auto)immune Th1/Th17 cell responses in female rat dLNs. On the contrary, the frequency of IL-4+ T cells was lower in female than in male rat dLNs. Consistently, the ratio of serum levels of collagen-specific IgG2a (IFN-γ-dependent, with an important pathogenic role in CIA) and IgG1 (IL-4-dependent) was shifted towards IgG2a in female compared with male rats. As a whole, the study suggests that sexual dimorphism in the control of T cell activation/polarization could contribute to sex bias in the susceptibility to CIA. Moreover, the study advises the use of animals of both sexes in the preclinical testing of new drugs for rheumatoid arthritis.",
journal = "Experimental and Molecular Pathology",
title = "Collagen-induced arthritis in Dark Agouti rats as a model for study of immunological sexual dimorphisms in the human disease.",
number = "1",
volume = "105",
doi = "10.1016/j.yexmp.2018.05.007",
pages = "10-22"
}

Dimitrijević, M., Arsenović-Ranin, N., Bufan, B., Nacka-Aleksić, M., Lazarević Macanović, M., Milovanović, P., Đurić, M., Sopta, J.,& Leposavić, G.. (2018). Collagen-induced arthritis in Dark Agouti rats as a model for study of immunological sexual dimorphisms in the human disease.. in Experimental and Molecular Pathology, 105(1), 10-22.
https://doi.org/10.1016/j.yexmp.2018.05.007

Dimitrijević M, Arsenović-Ranin N, Bufan B, Nacka-Aleksić M, Lazarević Macanović M, Milovanović P, Đurić M, Sopta J, Leposavić G. Collagen-induced arthritis in Dark Agouti rats as a model for study of immunological sexual dimorphisms in the human disease.. in Experimental and Molecular Pathology. 2018;105(1):10-22.
doi:10.1016/j.yexmp.2018.05.007 .

Dimitrijević, Mirjana, Arsenović-Ranin, Nevena, Bufan, Biljana, Nacka-Aleksić, Mirjana, Lazarević Macanović, Mirjana, Milovanović, Petar, Đurić, Marija, Sopta, Jelena, Leposavić, Gordana, "Collagen-induced arthritis in Dark Agouti rats as a model for study of immunological sexual dimorphisms in the human disease." in Experimental and Molecular Pathology, 105, no. 1 (2018):10-22,
https://doi.org/10.1016/j.yexmp.2018.05.007 . .

TY  - JOUR
AU  - Lazić, Marko M
AU  - Carretero, Miguel A
AU  - Mihajilov-Krstev, Tatjana M
AU  - Lazarević-Macanović, Mirjana
AU  - Krstić, Nikola E
AU  - Crnobrnja-Isailović, Jelka M.
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1077
AB  - Skin lesions frequently present in adult lizards may be due to a variety of causes, both physical and infectious, including excessively high humidity and environmental temperature, malnutrition, concurrent disease etc. On the other hand, skin lesions in lizards could be simple evidence of various behavioural patterns and biotic interactions. However, studies on frequencies of dermal lesions and their anatomical and environmental correlates in lacertid lizards are rare. Here, we use Podarcis muralis to analyse the relations between occurrence of ectodermal lesions and three possible indicators of environmental stress (body condition index - BCI, infestation by ticks and tail condition) by evaluating differences among local populations at uni- and multivariate level. Our results showed that BCI, together with body size and sexual size dimorphism, varied between populations but had no direct influence on the presence of lesions. Males had higher frequencies of lesions and ticks but lower frequencies of broken tails than females. All three parameters varied between sites likely due to differences in predation/parasite exposures and agonistic interactions with conspecifics between sexes and populations. Results of multivariate analyses suggested that the occurrence of lesions is decoupled from the other morphological stress indicators. Detected associations indicated that relations between presence of lesions and other analysed variables are rather complex. Directions for further research on ectodermal lesions in lacertid lizards are provided.
T2  - Amphibia-Reptilia
T1  - Incidence patterns of ectodermic lesions in wild populations of Common Wall Lizard (Podarcis muralis)
IS  - 3-4
VL  - 33
SP  - 115
EP  - 336
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1077
ER  - 

@article{
author = "Lazić, Marko M and Carretero, Miguel A and Mihajilov-Krstev, Tatjana M and Lazarević-Macanović, Mirjana and Krstić, Nikola E and Crnobrnja-Isailović, Jelka M.",
year = "2012",
abstract = "Skin lesions frequently present in adult lizards may be due to a variety of causes, both physical and infectious, including excessively high humidity and environmental temperature, malnutrition, concurrent disease etc. On the other hand, skin lesions in lizards could be simple evidence of various behavioural patterns and biotic interactions. However, studies on frequencies of dermal lesions and their anatomical and environmental correlates in lacertid lizards are rare. Here, we use Podarcis muralis to analyse the relations between occurrence of ectodermal lesions and three possible indicators of environmental stress (body condition index - BCI, infestation by ticks and tail condition) by evaluating differences among local populations at uni- and multivariate level. Our results showed that BCI, together with body size and sexual size dimorphism, varied between populations but had no direct influence on the presence of lesions. Males had higher frequencies of lesions and ticks but lower frequencies of broken tails than females. All three parameters varied between sites likely due to differences in predation/parasite exposures and agonistic interactions with conspecifics between sexes and populations. Results of multivariate analyses suggested that the occurrence of lesions is decoupled from the other morphological stress indicators. Detected associations indicated that relations between presence of lesions and other analysed variables are rather complex. Directions for further research on ectodermal lesions in lacertid lizards are provided.",
journal = "Amphibia-Reptilia",
title = "Incidence patterns of ectodermic lesions in wild populations of Common Wall Lizard (Podarcis muralis)",
number = "3-4",
volume = "33",
pages = "115-336",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1077"
}

Lazić, M. M., Carretero, M. A., Mihajilov-Krstev, T. M., Lazarević-Macanović, M., Krstić, N. E.,& Crnobrnja-Isailović, J. M.. (2012). Incidence patterns of ectodermic lesions in wild populations of Common Wall Lizard (Podarcis muralis). in Amphibia-Reptilia, 33(3-4), 115-336.
https://hdl.handle.net/21.15107/rcub_ibiss_1077

Lazić MM, Carretero MA, Mihajilov-Krstev TM, Lazarević-Macanović M, Krstić NE, Crnobrnja-Isailović JM. Incidence patterns of ectodermic lesions in wild populations of Common Wall Lizard (Podarcis muralis). in Amphibia-Reptilia. 2012;33(3-4):115-336.
https://hdl.handle.net/21.15107/rcub_ibiss_1077 .

Lazić, Marko M, Carretero, Miguel A, Mihajilov-Krstev, Tatjana M, Lazarević-Macanović, Mirjana, Krstić, Nikola E, Crnobrnja-Isailović, Jelka M., "Incidence patterns of ectodermic lesions in wild populations of Common Wall Lizard (Podarcis muralis)" in Amphibia-Reptilia, 33, no. 3-4 (2012):115-336,
https://hdl.handle.net/21.15107/rcub_ibiss_1077 .