TY  - CONF
AU  - Tanić, Nikola
AU  - Blagojević, Danijela
AU  - Milanović, Ivana
AU  - Imširović, Mirela
AU  - Lazić, Sandra
AU  - Maksimović, Zlatko
AU  - Tanić, Nasta
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6328
AB  - Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged at the end of 2019 and caused COVID-19 pandemic. This coronavirus disease pandemic demonstrated the importance of diagnostic testing in disease outbreak monitoring and control. So, reliable and accurate testing for SARS-CoV-2 was the principal prerequisite for preventing the spread of COVID-19.
Methods: Real Time RT-PCR (RT-qPCR) unquestionably represent the most reliable, rapid and sensitive method for detection of SARS-CoV-2 RNA. However, there are numerous different assays, protocols, reagents, instruments and result analysis methods in use without certified standards, standardized RNA extraction and reporting procedures. Therefore, in practice, the reliability of RT-qPCR results depends on a number of parameters that include sample collection and processing, method of RNA extraction, choice of assay, efficiency of assay, choice of instrument, analysis method as well as operator intervention.
Results: Here we present comparative analyses of the efficiency and sensitivity of 10 different amplification assays, as well as the relevance of manual RNA extractions compared to automatic one. Our results revealed that manual viral RNA extraction should be a method of choice for high sensitivity. In addition, amplification assays targeting three SARS-CoV-2 genes are much more efficient from those targeting one.
Conclusion: Unfortunately, RT-qPCR is almost exclusively used as qualitative diagnostic test for SARSCoV-2. We think that the ideal testing regimen would involve not just qualitative detection of SARS-CoV-2 but reliable and meaningful quantitative reporting of viral load.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
C3  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Diagnostic testing for SARS-COV-2 by Real-time RT-PCR
IS  - 99
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6328
ER  - 

@conference{
author = "Tanić, Nikola and Blagojević, Danijela and Milanović, Ivana and Imširović, Mirela and Lazić, Sandra and Maksimović, Zlatko and Tanić, Nasta",
year = "2023",
abstract = "Introduction: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged at the end of 2019 and caused COVID-19 pandemic. This coronavirus disease pandemic demonstrated the importance of diagnostic testing in disease outbreak monitoring and control. So, reliable and accurate testing for SARS-CoV-2 was the principal prerequisite for preventing the spread of COVID-19.
Methods: Real Time RT-PCR (RT-qPCR) unquestionably represent the most reliable, rapid and sensitive method for detection of SARS-CoV-2 RNA. However, there are numerous different assays, protocols, reagents, instruments and result analysis methods in use without certified standards, standardized RNA extraction and reporting procedures. Therefore, in practice, the reliability of RT-qPCR results depends on a number of parameters that include sample collection and processing, method of RNA extraction, choice of assay, efficiency of assay, choice of instrument, analysis method as well as operator intervention.
Results: Here we present comparative analyses of the efficiency and sensitivity of 10 different amplification assays, as well as the relevance of manual RNA extractions compared to automatic one. Our results revealed that manual viral RNA extraction should be a method of choice for high sensitivity. In addition, amplification assays targeting three SARS-CoV-2 genes are much more efficient from those targeting one.
Conclusion: Unfortunately, RT-qPCR is almost exclusively used as qualitative diagnostic test for SARSCoV-2. We think that the ideal testing regimen would involve not just qualitative detection of SARS-CoV-2 but reliable and meaningful quantitative reporting of viral load.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Diagnostic testing for SARS-COV-2 by Real-time RT-PCR",
number = "99",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6328"
}

Tanić, N., Blagojević, D., Milanović, I., Imširović, M., Lazić, S., Maksimović, Z.,& Tanić, N.. (2023). Diagnostic testing for SARS-COV-2 by Real-time RT-PCR. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade.(99).
https://hdl.handle.net/21.15107/rcub_ibiss_6328

Tanić N, Blagojević D, Milanović I, Imširović M, Lazić S, Maksimović Z, Tanić N. Diagnostic testing for SARS-COV-2 by Real-time RT-PCR. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;(99).
https://hdl.handle.net/21.15107/rcub_ibiss_6328 .

Tanić, Nikola, Blagojević, Danijela, Milanović, Ivana, Imširović, Mirela, Lazić, Sandra, Maksimović, Zlatko, Tanić, Nasta, "Diagnostic testing for SARS-COV-2 by Real-time RT-PCR" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia, no. 99 (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_6328 .