TY  - JOUR
AU  - Podgorac, Jelena
AU  - Pešić, Vesna
AU  - Pavković, Željko
AU  - Martać, Ljiljana
AU  - Kanazir, Selma
AU  - Filipović, Ljupka
AU  - Sekulić, Slobodan
PY  - 2016
UR  - http://www.journals.elsevier.com/behavioural-brain-research/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5456
AB  - Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.
PB  - Amsterdam : Elsevier
T2  - Behavioural Brain Research
T1  - Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development
VL  - 311
DO  - 10.1016/j.bbr.2016.05.023
SP  - 99
EP  - 109
ER  - 

@article{
author = "Podgorac, Jelena and Pešić, Vesna and Pavković, Željko and Martać, Ljiljana and Kanazir, Selma and Filipović, Ljupka and Sekulić, Slobodan",
year = "2016",
abstract = "Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.",
publisher = "Amsterdam : Elsevier",
journal = "Behavioural Brain Research",
title = "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development",
volume = "311",
doi = "10.1016/j.bbr.2016.05.023",
pages = "99-109"
}

Podgorac, J., Pešić, V., Pavković, Ž., Martać, L., Kanazir, S., Filipović, L.,& Sekulić, S.. (2016). Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research
Amsterdam : Elsevier., 311, 99-109.
https://doi.org/10.1016/j.bbr.2016.05.023

Podgorac J, Pešić V, Pavković Ž, Martać L, Kanazir S, Filipović L, Sekulić S. Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research. 2016;311:99-109.
doi:10.1016/j.bbr.2016.05.023 .

Podgorac, Jelena, Pešić, Vesna, Pavković, Željko, Martać, Ljiljana, Kanazir, Selma, Filipović, Ljupka, Sekulić, Slobodan, "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development" in Behavioural Brain Research, 311 (2016):99-109,
https://doi.org/10.1016/j.bbr.2016.05.023 . .

TY  - JOUR
AU  - Podgorac, Jelena
AU  - Pešić, Vesna
AU  - Pavković, Željko
AU  - Martać, Ljiljana
AU  - Kanazir, Selma
AU  - Filipović, Ljupka
AU  - Sekulić, Slobodan
PY  - 2016
UR  - http://www.journals.elsevier.com/behavioural-brain-research/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5455
AB  - Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.
PB  - Amsterdam : Elsevier
T2  - Behavioural Brain Research
T1  - Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development
VL  - 311
DO  - 10.1016/j.bbr.2016.05.023
SP  - 99
EP  - 109
ER  - 

@article{
author = "Podgorac, Jelena and Pešić, Vesna and Pavković, Željko and Martać, Ljiljana and Kanazir, Selma and Filipović, Ljupka and Sekulić, Slobodan",
year = "2016",
abstract = "Clinical research has identified developmental delay and physical malformations in children prenatally exposed to the antiepileptic drug (AED) valproic acid (VPA). However, the early signs of neurodevelopmental deficits, their evolution during postnatal development and growth, and the dose effects of VPA are not well understood. The present study aimed to examine the influence of maternal exposure to a wide dose range (50, 100, 200 and 400 mg/kg/day) of VPA during breeding and gestation on early physical and neuromotor development in mice offspring. Body weight gain, eye opening, the surface righting reflex (SRR) and tail suspension test (TST) were examined in the offspring at postnatal days 5, 10 and 15. We observed that: (1) all tested doses of VPA reduced the body weight of the offspring and the timing of eye opening; (2) offspring exposed to VPA displayed immature forms of righting and required more time to complete the SRR; (3) latency for the first immobilization in the TST is shorter in offspring exposed to higher doses of VPA; however, mice in all groups exposed to VPA exhibited atypical changes in this parameter during the examined period of maturation; (4) irregularities in swinging and curling activities were observed in animals exposed to higher doses of VPA. This study points to delayed somatic development and postponed maturation of the motor system in all of the offspring prenatally exposed to VPA, with stronger effects observed at higher doses. The results implicate that the strategy of continuous monitoring of general health and achievements in motor milestones during the early postnatal development in prenatally VPA-exposed offspring, irrespectively of the dose applied, could help to recognize early developmental irregularities.",
publisher = "Amsterdam : Elsevier",
journal = "Behavioural Brain Research",
title = "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development",
volume = "311",
doi = "10.1016/j.bbr.2016.05.023",
pages = "99-109"
}

Podgorac, J., Pešić, V., Pavković, Ž., Martać, L., Kanazir, S., Filipović, L.,& Sekulić, S.. (2016). Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research
Amsterdam : Elsevier., 311, 99-109.
https://doi.org/10.1016/j.bbr.2016.05.023

Podgorac J, Pešić V, Pavković Ž, Martać L, Kanazir S, Filipović L, Sekulić S. Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development. in Behavioural Brain Research. 2016;311:99-109.
doi:10.1016/j.bbr.2016.05.023 .

Podgorac, Jelena, Pešić, Vesna, Pavković, Željko, Martać, Ljiljana, Kanazir, Selma, Filipović, Ljupka, Sekulić, Slobodan, "Early physical and motor development of mouse offspring exposed to valproic acid throughout intrauterine development" in Behavioural Brain Research, 311 (2016):99-109,
https://doi.org/10.1016/j.bbr.2016.05.023 . .

TY  - CONF
AU  - Filipović, Ljupka
AU  - Ristić, Nina
AU  - Perišić, Vojislav
AU  - Ignjatović, Đurđica
AU  - Đurović, Jelena
AU  - Obradović, Svetlana
AU  - Stevanović, Snežana
AU  - Stamenković, Gorana
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6768
AB  - Celiac disease (CD) is an intestinal inflammatory disorder triggered by gluten ingestion. It's strongly associated with HLA-DQ2 or HLA-DQ8 haplotypes: the absence of HLA-DQ haplotypes suggests that CD is unlikely. Lactose intolerance (LI) is gastrointestinal disorder caused by reduced lactase activity and is associated with C/T13910 polymorphism upstream of the lactase gene. Similar gastrointestinal symptoms in both diseases cause difficulties in establishing the correct diagnosis.
The aim of this study was to evaluate the importance of genetic analysis for clinical discrimination between CD and LI. The study group consisted of 70 patients from Serbia, with gastrointestinal symptoms suggesting CD. Control group for CD (62 individual) was previously published, while a novel group of 54 healthy individuals without gastrointestinal symptoms was genotyped for C/T13910. HLA haplotypes were established using reverse linc blot method. Polymorphism on C/113910 locus for lactose intolerance was genotyped by TaqMan assay.
As much as 60% of patients (significantly higher compared to published controls, p=0.026) in the studied group were positive for either HLA-DQ2 (49%) or HLA-DQ8 (11%) haplotypes, while 31% of patients were negative for both haplotypes. Complex HLA-DQ2/DQ8 haplotypes had 3% of patients. Remaining 6% of patients were not successfully genotyped by the method. For C/T13910, 51% of patients were homozygous for C allele (not significantly different compared to healthy controls, p=0.96). In addition, 41% of HLA negative patients, suggested the absence of CD, were positive for genetic predisposition for LI.
Our results imply importance of performing genetic analysis for better distinguishing between CD and LI.
PB  - Belgrade: Serbian Genetic Society
C3  - 11th Balkan Congress of Human Genetics; 2015 Sep 17-20; Belgrade, Serbia
T1  - Genetic predisposition for celiac disease and lactose intolerance in patient with gastrointestinal ailments – study in Serbia
IS  - 49
EP  - 50
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6768
ER  - 

@conference{
author = "Filipović, Ljupka and Ristić, Nina and Perišić, Vojislav and Ignjatović, Đurđica and Đurović, Jelena and Obradović, Svetlana and Stevanović, Snežana and Stamenković, Gorana",
year = "2015",
abstract = "Celiac disease (CD) is an intestinal inflammatory disorder triggered by gluten ingestion. It's strongly associated with HLA-DQ2 or HLA-DQ8 haplotypes: the absence of HLA-DQ haplotypes suggests that CD is unlikely. Lactose intolerance (LI) is gastrointestinal disorder caused by reduced lactase activity and is associated with C/T13910 polymorphism upstream of the lactase gene. Similar gastrointestinal symptoms in both diseases cause difficulties in establishing the correct diagnosis.
The aim of this study was to evaluate the importance of genetic analysis for clinical discrimination between CD and LI. The study group consisted of 70 patients from Serbia, with gastrointestinal symptoms suggesting CD. Control group for CD (62 individual) was previously published, while a novel group of 54 healthy individuals without gastrointestinal symptoms was genotyped for C/T13910. HLA haplotypes were established using reverse linc blot method. Polymorphism on C/113910 locus for lactose intolerance was genotyped by TaqMan assay.
As much as 60% of patients (significantly higher compared to published controls, p=0.026) in the studied group were positive for either HLA-DQ2 (49%) or HLA-DQ8 (11%) haplotypes, while 31% of patients were negative for both haplotypes. Complex HLA-DQ2/DQ8 haplotypes had 3% of patients. Remaining 6% of patients were not successfully genotyped by the method. For C/T13910, 51% of patients were homozygous for C allele (not significantly different compared to healthy controls, p=0.96). In addition, 41% of HLA negative patients, suggested the absence of CD, were positive for genetic predisposition for LI.
Our results imply importance of performing genetic analysis for better distinguishing between CD and LI.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "11th Balkan Congress of Human Genetics; 2015 Sep 17-20; Belgrade, Serbia",
title = "Genetic predisposition for celiac disease and lactose intolerance in patient with gastrointestinal ailments – study in Serbia",
number = "49",
pages = "50",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6768"
}

Filipović, L., Ristić, N., Perišić, V., Ignjatović, Đ., Đurović, J., Obradović, S., Stevanović, S.,& Stamenković, G.. (2015). Genetic predisposition for celiac disease and lactose intolerance in patient with gastrointestinal ailments – study in Serbia. in 11th Balkan Congress of Human Genetics; 2015 Sep 17-20; Belgrade, Serbia
Belgrade: Serbian Genetic Society.(49).
https://hdl.handle.net/21.15107/rcub_ibiss_6768

Filipović L, Ristić N, Perišić V, Ignjatović Đ, Đurović J, Obradović S, Stevanović S, Stamenković G. Genetic predisposition for celiac disease and lactose intolerance in patient with gastrointestinal ailments – study in Serbia. in 11th Balkan Congress of Human Genetics; 2015 Sep 17-20; Belgrade, Serbia. 2015;(49):null-50.
https://hdl.handle.net/21.15107/rcub_ibiss_6768 .

Filipović, Ljupka, Ristić, Nina, Perišić, Vojislav, Ignjatović, Đurđica, Đurović, Jelena, Obradović, Svetlana, Stevanović, Snežana, Stamenković, Gorana, "Genetic predisposition for celiac disease and lactose intolerance in patient with gastrointestinal ailments – study in Serbia" in 11th Balkan Congress of Human Genetics; 2015 Sep 17-20; Belgrade, Serbia, no. 49 (2015),
https://hdl.handle.net/21.15107/rcub_ibiss_6768 .