TY  - JOUR
AU  - Avdović, Edina H.
AU  - Petrović, Isidora P.
AU  - Stevanović, Milena J.
AU  - Saso, Luciano
AU  - Dimitrić Marković, Jasmina M.
AU  - Filipović, Nenad D.
AU  - Živić, Miroslav Ž.
AU  - Cvetić Antić, Tijana N.
AU  - Žižić, Milan V.
AU  - Todorović, Nataša
AU  - Vukić, Milena
AU  - Trifunović, Srećko R.
AU  - Marković, Zoran S.
PY  - 2021
UR  - https://www.hindawi.com/journals/omcl/2021/8849568/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4244
AB  - Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, 1H NMR, 13C NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards •OH and -•OOH radicals and anti-ABTS (2,2 ′ -Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells’ viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.
PB  - Hindawi Limited
T2  - Oxidative Medicine and Cellular Longevity
T1  - Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes
VL  - 2021
DO  - 10.1155/2021/8849568
SP  - 8849568
ER  - 

@article{
author = "Avdović, Edina H. and Petrović, Isidora P. and Stevanović, Milena J. and Saso, Luciano and Dimitrić Marković, Jasmina M. and Filipović, Nenad D. and Živić, Miroslav Ž. and Cvetić Antić, Tijana N. and Žižić, Milan V. and Todorović, Nataša and Vukić, Milena and Trifunović, Srećko R. and Marković, Zoran S.",
year = "2021",
abstract = "Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, 1H NMR, 13C NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards •OH and -•OOH radicals and anti-ABTS (2,2 ′ -Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells’ viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.",
publisher = "Hindawi Limited",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes",
volume = "2021",
doi = "10.1155/2021/8849568",
pages = "8849568"
}

Avdović, E. H., Petrović, I. P., Stevanović, M. J., Saso, L., Dimitrić Marković, J. M., Filipović, N. D., Živić, M. Ž., Cvetić Antić, T. N., Žižić, M. V., Todorović, N., Vukić, M., Trifunović, S. R.,& Marković, Z. S.. (2021). Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity
Hindawi Limited., 2021, 8849568.
https://doi.org/10.1155/2021/8849568

Avdović EH, Petrović IP, Stevanović MJ, Saso L, Dimitrić Marković JM, Filipović ND, Živić MŽ, Cvetić Antić TN, Žižić MV, Todorović N, Vukić M, Trifunović SR, Marković ZS. Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity. 2021;2021:8849568.
doi:10.1155/2021/8849568 .

Avdović, Edina H., Petrović, Isidora P., Stevanović, Milena J., Saso, Luciano, Dimitrić Marković, Jasmina M., Filipović, Nenad D., Živić, Miroslav Ž., Cvetić Antić, Tijana N., Žižić, Milan V., Todorović, Nataša, Vukić, Milena, Trifunović, Srećko R., Marković, Zoran S., "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes" in Oxidative Medicine and Cellular Longevity, 2021 (2021):8849568,
https://doi.org/10.1155/2021/8849568 . .

TY  - JOUR
AU  - Aleksić, Marija
AU  - Kalezić, Anđelika
AU  - Saso, Luciano
AU  - Janković, Aleksandra
AU  - Korać, Bato
AU  - Korać, Aleksandra
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4216
AB  - Brown adipose tissue (BAT) is important for maintaining whole-body metabolic and energy homeostasis. However, the effects of hypothyroidism, one of the most common diseases worldwide, which increases the risk of several metabolic disorders, on BAT redox and metabolic homeostasis remain mostly unknown. We aimed to investigate the dynamics of protein expression, enzyme activity, and localization of antioxidant defense (AD) enzymes in rat interscapular BAT upon induction of hypothyroidism by antithyroid drug methimazole for 7, 15, and 21 days. Our results showed an increased protein expression of CuZn-and Mn-superoxide dismutase, catalase, glutamyl– cysteine ligase, thioredoxin, total glutathione content, and activity of catalase and thioredoxin reductase in hypothyroid rats, compared to euthyroid control. Concomitant with the increase in AD, newly established nuclear, mitochondrial, and peroxisomal localization of AD enzymes was found. Hypothyroidism also potentiated associations between mitochondria, peroxisomes, and lipid bodies, creating specific structural–functional units. Moreover, hypothyroidism induced protein expression and nuclear translocation of a master regulator of redox-metabolic homeostasis, nuclear factor erythroid 2-related factor 2 (Nrf2), and an increased amount of 4-hydroxynonenal (4-HNE) protein adducts. The results indicate that spatiotemporal overlap in the remodeling of AD is orchestrated by Nrf2, implicating the role of 4-HNE in this process and suggesting the potential mechanism of redox-structural remodeling during BAT adaptation in hypothyroidism.
PB  - MDPI AG
T2  - Antioxidants
T1  - The unity of redox and structural remodeling of brown adipose tissue in hypothyroidism
IS  - 4
VL  - 10
DO  - 10.3390/antiox10040591
SP  - 591
ER  - 

@article{
author = "Aleksić, Marija and Kalezić, Anđelika and Saso, Luciano and Janković, Aleksandra and Korać, Bato and Korać, Aleksandra",
year = "2021",
abstract = "Brown adipose tissue (BAT) is important for maintaining whole-body metabolic and energy homeostasis. However, the effects of hypothyroidism, one of the most common diseases worldwide, which increases the risk of several metabolic disorders, on BAT redox and metabolic homeostasis remain mostly unknown. We aimed to investigate the dynamics of protein expression, enzyme activity, and localization of antioxidant defense (AD) enzymes in rat interscapular BAT upon induction of hypothyroidism by antithyroid drug methimazole for 7, 15, and 21 days. Our results showed an increased protein expression of CuZn-and Mn-superoxide dismutase, catalase, glutamyl– cysteine ligase, thioredoxin, total glutathione content, and activity of catalase and thioredoxin reductase in hypothyroid rats, compared to euthyroid control. Concomitant with the increase in AD, newly established nuclear, mitochondrial, and peroxisomal localization of AD enzymes was found. Hypothyroidism also potentiated associations between mitochondria, peroxisomes, and lipid bodies, creating specific structural–functional units. Moreover, hypothyroidism induced protein expression and nuclear translocation of a master regulator of redox-metabolic homeostasis, nuclear factor erythroid 2-related factor 2 (Nrf2), and an increased amount of 4-hydroxynonenal (4-HNE) protein adducts. The results indicate that spatiotemporal overlap in the remodeling of AD is orchestrated by Nrf2, implicating the role of 4-HNE in this process and suggesting the potential mechanism of redox-structural remodeling during BAT adaptation in hypothyroidism.",
publisher = "MDPI AG",
journal = "Antioxidants",
title = "The unity of redox and structural remodeling of brown adipose tissue in hypothyroidism",
number = "4",
volume = "10",
doi = "10.3390/antiox10040591",
pages = "591"
}

Aleksić, M., Kalezić, A., Saso, L., Janković, A., Korać, B.,& Korać, A.. (2021). The unity of redox and structural remodeling of brown adipose tissue in hypothyroidism. in Antioxidants
MDPI AG., 10(4), 591.
https://doi.org/10.3390/antiox10040591

Aleksić M, Kalezić A, Saso L, Janković A, Korać B, Korać A. The unity of redox and structural remodeling of brown adipose tissue in hypothyroidism. in Antioxidants. 2021;10(4):591.
doi:10.3390/antiox10040591 .

Aleksić, Marija, Kalezić, Anđelika, Saso, Luciano, Janković, Aleksandra, Korać, Bato, Korać, Aleksandra, "The unity of redox and structural remodeling of brown adipose tissue in hypothyroidism" in Antioxidants, 10, no. 4 (2021):591,
https://doi.org/10.3390/antiox10040591 . .

TY  - JOUR
AU  - Janković, Aleksandra
AU  - Saso, Luciano
AU  - Korać, Aleksandra
AU  - Korać, Bato
PY  - 2019
UR  - https://www.hindawi.com/journals/omcl/2019/2471312/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3285
AB  - Accumulation of oxidative insults on molecular and supramolecular levels could compromise renewal potency and architecture in the aging skin. To examine and compare morphological and ultrastructural changes with redox alterations during chronological skin aging, activities of antioxidant defense (AD) enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), thioredoxin reductase (TR), and methionine sulfoxide reductase A (MsrA), and the markers of oxidative damage of biomolecules—4-hydroxynonenal (HNE) and 8-oxoguanine (8-oxoG)—were examined in the rat skin during life (from 3 days to 21 months). As compared to adult 3-month-old skin, higher activities of CAT, GSH-Px, and GR and a decline in expression of MsrA are found in 21-month-old skin. These changes correspond to degenerative changes at structural and ultrastructural levels in epidermal and dermal compartments, low proliferation capacity, and higher levels of HNE-modified protein aldehydes (particularly in basal lamina) and 8-oxoG positivity in nuclei and mitochondria in the sebaceous glands and root sheath. In 3-day-old skin, higher activities of AD enzymes (SOD, CAT, GR, and TR) and MsrA expression correspond to intensive postnatal development and proliferation. In contrast to 21-month-old skin, a high level of HNE in young skin is not accompanied by 8-oxoG positivity or any morphological disturbances. Observed results indicate that increased activity of AD enzymes in elderly rat skin represents the compensatory response to accumulated oxidative damage of DNA and proteins, accompanied by attenuated repair and proliferative capacity, but in young rats the redox changes are necessary and inherent with processes which occur during postnatal skin development. Мorphological and ultrastructurаl changes are in line with the redox profile in the skin of young and old rats.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging
VL  - 2019
DO  - 10.1155/2019/2471312
SP  - 2471312
ER  - 

@article{
author = "Janković, Aleksandra and Saso, Luciano and Korać, Aleksandra and Korać, Bato",
year = "2019",
abstract = "Accumulation of oxidative insults on molecular and supramolecular levels could compromise renewal potency and architecture in the aging skin. To examine and compare morphological and ultrastructural changes with redox alterations during chronological skin aging, activities of antioxidant defense (AD) enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), thioredoxin reductase (TR), and methionine sulfoxide reductase A (MsrA), and the markers of oxidative damage of biomolecules—4-hydroxynonenal (HNE) and 8-oxoguanine (8-oxoG)—were examined in the rat skin during life (from 3 days to 21 months). As compared to adult 3-month-old skin, higher activities of CAT, GSH-Px, and GR and a decline in expression of MsrA are found in 21-month-old skin. These changes correspond to degenerative changes at structural and ultrastructural levels in epidermal and dermal compartments, low proliferation capacity, and higher levels of HNE-modified protein aldehydes (particularly in basal lamina) and 8-oxoG positivity in nuclei and mitochondria in the sebaceous glands and root sheath. In 3-day-old skin, higher activities of AD enzymes (SOD, CAT, GR, and TR) and MsrA expression correspond to intensive postnatal development and proliferation. In contrast to 21-month-old skin, a high level of HNE in young skin is not accompanied by 8-oxoG positivity or any morphological disturbances. Observed results indicate that increased activity of AD enzymes in elderly rat skin represents the compensatory response to accumulated oxidative damage of DNA and proteins, accompanied by attenuated repair and proliferative capacity, but in young rats the redox changes are necessary and inherent with processes which occur during postnatal skin development. Мorphological and ultrastructurаl changes are in line with the redox profile in the skin of young and old rats.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging",
volume = "2019",
doi = "10.1155/2019/2471312",
pages = "2471312"
}

Janković, A., Saso, L., Korać, A.,& Korać, B.. (2019). Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging. in Oxidative Medicine and Cellular Longevity, 2019, 2471312.
https://doi.org/10.1155/2019/2471312

Janković A, Saso L, Korać A, Korać B. Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging. in Oxidative Medicine and Cellular Longevity. 2019;2019:2471312.
doi:10.1155/2019/2471312 .

Janković, Aleksandra, Saso, Luciano, Korać, Aleksandra, Korać, Bato, "Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging" in Oxidative Medicine and Cellular Longevity, 2019 (2019):2471312,
https://doi.org/10.1155/2019/2471312 . .