TY  - CONF
AU  - Dmitrović, Slavica
AU  - Perišić, Mirjana
AU  - Stojić, Andreja
AU  - Živković, Suzana
AU  - Božunović, Jelena
AU  - Nestorović Živković, Jasmina
AU  - Aničić, Neda
AU  - Ristić, Mihailo
AU  - Mišić, Danijela
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6185
AB  - The possibility of using essential oil (EO) of Nepeta rtanjensis and N. cataria as bioherbicides against Ambrosia artemisiifolia L., an important weed and allergen species, was explored. In vitro culture experimental system of A. artemisiifolia has been established and ragweed shoots were exposed to the atmosphere enriched with volatile compounds (VOCs) emitted from EOs. N. rtanjensis EO was characterized by high amounts of 4aα,7α,7aβ-nepetalactone (trans,cis-nepetalactone), while N. cataria EO possessed high amounts of 4aα,7α,7aα-nepetalactone (cis,trans-nepetalactone). Reduction in A. artemisiifolia shoots growth and their discoloration, in the presence of N. rtanjensis and N. cataria EOs (2% and 4%, final nepetalactone concentrations) was observed after 2 weeks of exposure. An antioxidative defense system of A. artemisiifolia was activated by increasing peroxidases activity or by inactivating catalases and superoxide dismutases activity. Overall, stronger inhibitory effect on shoot growth, CAT activity, and stimulating POX activity was observed for N. cataria EO. N. rtanjensis EO was more efficient in inhibiting rooting and root growth, and in suppressing SOD activity. Therefore, EOs of N. rtanjensis and N. cataria might be recommended as potential bioherbicides against highly allergenic
and weed species A. artemisiifolia. Further exploration of EOs’ mode of action, confirmation of phytotoxicity in field conditions, and feasibility assessment for the commercial production and application in agricultural practice, is the course of our further work.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils
SP  - 159
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6185
ER  - 

@conference{
author = "Dmitrović, Slavica and Perišić, Mirjana and Stojić, Andreja and Živković, Suzana and Božunović, Jelena and Nestorović Živković, Jasmina and Aničić, Neda and Ristić, Mihailo and Mišić, Danijela",
year = "2015",
abstract = "The possibility of using essential oil (EO) of Nepeta rtanjensis and N. cataria as bioherbicides against Ambrosia artemisiifolia L., an important weed and allergen species, was explored. In vitro culture experimental system of A. artemisiifolia has been established and ragweed shoots were exposed to the atmosphere enriched with volatile compounds (VOCs) emitted from EOs. N. rtanjensis EO was characterized by high amounts of 4aα,7α,7aβ-nepetalactone (trans,cis-nepetalactone), while N. cataria EO possessed high amounts of 4aα,7α,7aα-nepetalactone (cis,trans-nepetalactone). Reduction in A. artemisiifolia shoots growth and their discoloration, in the presence of N. rtanjensis and N. cataria EOs (2% and 4%, final nepetalactone concentrations) was observed after 2 weeks of exposure. An antioxidative defense system of A. artemisiifolia was activated by increasing peroxidases activity or by inactivating catalases and superoxide dismutases activity. Overall, stronger inhibitory effect on shoot growth, CAT activity, and stimulating POX activity was observed for N. cataria EO. N. rtanjensis EO was more efficient in inhibiting rooting and root growth, and in suppressing SOD activity. Therefore, EOs of N. rtanjensis and N. cataria might be recommended as potential bioherbicides against highly allergenic
and weed species A. artemisiifolia. Further exploration of EOs’ mode of action, confirmation of phytotoxicity in field conditions, and feasibility assessment for the commercial production and application in agricultural practice, is the course of our further work.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils",
pages = "159",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6185"
}

Dmitrović, S., Perišić, M., Stojić, A., Živković, S., Božunović, J., Nestorović Živković, J., Aničić, N., Ristić, M.,& Mišić, D.. (2015). The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 159.
https://hdl.handle.net/21.15107/rcub_ibiss_6185

Dmitrović S, Perišić M, Stojić A, Živković S, Božunović J, Nestorović Živković J, Aničić N, Ristić M, Mišić D. The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils. in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:159.
https://hdl.handle.net/21.15107/rcub_ibiss_6185 .

Dmitrović, Slavica, Perišić, Mirjana, Stojić, Andreja, Živković, Suzana, Božunović, Jelena, Nestorović Živković, Jasmina, Aničić, Neda, Ristić, Mihailo, Mišić, Danijela, "The oxidative stress in Ambrosia artemisiifolia L. shoots grown in vitro induced by Nepeta rtanjensis and N. cataria essential oils" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):159,
https://hdl.handle.net/21.15107/rcub_ibiss_6185 .

TY  - CONF
AU  - Nestorović Živković, Jasmina
AU  - Perišić, Mirjana
AU  - Stojić, Andreja
AU  - Živković, Suzana
AU  - Dmitrović, Slavica
AU  - Šiler, Branislav
AU  - Mišić, Danijela
PY  - 2015
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6188
AB  - Phytotoxic effects of volatile organic compounds (VOCs) from Nepeta species on agricultural and weed
species have been previously demonstrated, and are usually attributed to iridoid monoterpenes nepetalactones. Here we studied the effect of VOCs of three endemic Nepeta species, differing in their qualitative nepetalactone content, on seed germination and seedling growth of garden cress (Lepidium sativum L.). Garden cress was exposed to the atmosphere enriched with VOCs released from the leaf surface of three Nepeta species. Identification of VOCs was performed by headspace GC-MS, while the concentrations of nepetalactone in the atmosphere of culture vessels were detected by PTR-MS. Volatiles released from N. rtanjensis and N. sibirica shoots reduced seed germination and inhibited seedling growth of garden cress. Phytotoxic effect of N. rtanjensis and N. sibirica originates from the dominant bioactive compound of these species – nepetalactone. Stereochemistry of nepetalactone considerably determines its phytotoxic potential, trans,cis- isomer is more active than the cis,trans nepetalactone. Phytotoxic effect of nepetalactone is reflected through its effect on biochemical processes that are the consequence of plants disturbed antioxidative system: the inhibition of activities and changed profiles of peroxidases, catalase, Fe- and Cu/Zn-superoxide dismutase isoforms were observed.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
T1  - Volatile compounds of three Nepeta species inhibit seed germination, reduce seedling growth and induce oxidative stress in garden cress (Lepidium sativum L.)
SP  - 177
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6188
ER  - 

@conference{
author = "Nestorović Živković, Jasmina and Perišić, Mirjana and Stojić, Andreja and Živković, Suzana and Dmitrović, Slavica and Šiler, Branislav and Mišić, Danijela",
year = "2015",
abstract = "Phytotoxic effects of volatile organic compounds (VOCs) from Nepeta species on agricultural and weed
species have been previously demonstrated, and are usually attributed to iridoid monoterpenes nepetalactones. Here we studied the effect of VOCs of three endemic Nepeta species, differing in their qualitative nepetalactone content, on seed germination and seedling growth of garden cress (Lepidium sativum L.). Garden cress was exposed to the atmosphere enriched with VOCs released from the leaf surface of three Nepeta species. Identification of VOCs was performed by headspace GC-MS, while the concentrations of nepetalactone in the atmosphere of culture vessels were detected by PTR-MS. Volatiles released from N. rtanjensis and N. sibirica shoots reduced seed germination and inhibited seedling growth of garden cress. Phytotoxic effect of N. rtanjensis and N. sibirica originates from the dominant bioactive compound of these species – nepetalactone. Stereochemistry of nepetalactone considerably determines its phytotoxic potential, trans,cis- isomer is more active than the cis,trans nepetalactone. Phytotoxic effect of nepetalactone is reflected through its effect on biochemical processes that are the consequence of plants disturbed antioxidative system: the inhibition of activities and changed profiles of peroxidases, catalase, Fe- and Cu/Zn-superoxide dismutase isoforms were observed.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia",
title = "Volatile compounds of three Nepeta species inhibit seed germination, reduce seedling growth and induce oxidative stress in garden cress (Lepidium sativum L.)",
pages = "177",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6188"
}

Nestorović Živković, J., Perišić, M., Stojić, A., Živković, S., Dmitrović, S., Šiler, B.,& Mišić, D.. (2015). Volatile compounds of three Nepeta species inhibit seed germination, reduce seedling growth and induce oxidative stress in garden cress (Lepidium sativum L.). in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia
Belgrade: Serbian Plant Physiology Society., 177.
https://hdl.handle.net/21.15107/rcub_ibiss_6188

Nestorović Živković J, Perišić M, Stojić A, Živković S, Dmitrović S, Šiler B, Mišić D. Volatile compounds of three Nepeta species inhibit seed germination, reduce seedling growth and induce oxidative stress in garden cress (Lepidium sativum L.). in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia. 2015;:177.
https://hdl.handle.net/21.15107/rcub_ibiss_6188 .

Nestorović Živković, Jasmina, Perišić, Mirjana, Stojić, Andreja, Živković, Suzana, Dmitrović, Slavica, Šiler, Branislav, Mišić, Danijela, "Volatile compounds of three Nepeta species inhibit seed germination, reduce seedling growth and induce oxidative stress in garden cress (Lepidium sativum L.)" in Book of Abstracts: 2nd International Conference on Plant Biology, 21th Symposium of the Serbian Plant Physiology Society, and CОST Action FA1106 Quality Fruit Workshop; 2015 Jun 17-20; Petnica, Serbia (2015):177,
https://hdl.handle.net/21.15107/rcub_ibiss_6188 .

TY  - CONF
AU  - Skorić, Marijana
AU  - Todorović, Slađana
AU  - Ristić, Mihailo
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Živković, Suzana
AU  - Stojić, Andreja
AU  - Puač, Nevena
AU  - Kanellis, Angelos K
PY  - 2013
UR  - http://terpnet2013.pharm.auth.gr/site/TERPNET_BOOK_OF_ABSTRACTS.pdf
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6320
AB  - Cistus creticus subsp. creticus is a native plant of the Mediterranean region and it has been used since ancient times for its medicinal properties. Labdane type diterpenes are predominant compounds in leaves and stems of the C. creticus subsp. creticus, as well as in resin, while other terpenes, polyphenols and flavonoids contribute to chemical profile of this species. In vitro plant tissue culture is an attractive alternative approach to the traditional methods of plantations, as it offers a controlled supply of biochemicals independent of plant availability and more consistent product quality. In order to obtain applicable production of secondary metabolites of interest we have implemented in vitro culture of several clones of C. creticus, which were previously morphogenetically described. The ethanol extracts of obtained in vitro cultures were analyzed by GC-FID and GC-MS, and were characterized concerning the total phenolic and flavonoid contents. Labdane diterpenes were the most abundant compounds in above ground parts extracts, but absent from the root extracts. Head-space GC-MS was used to determine the profile of volatile compounds in the atmosphere of the culture vessels. α pinene, β pinene, and camphene were the most abundant ones. PTR-MS was used for further quantification of representative volatile compounds in the atmosphere of the culture vessels. Finally, the biological activity of obtained ethanol extract was studied. Microdilution method was used for investigation of antibacterial and antifungal activity. Almost all tested extract against human pathogenic bacteria showed better antibacterial activity than streptomycin, and much better antibacterial activity than ampicillin. All tested extracts also exhibited slightly higher or similar antifungal potential as bifonazole, and showed much better antifungal effect than ketoconazole. Antioxidant activity was determined by using DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, while cytotoxic activity was previously demonstrated by sulforhodamine B (SRB) assay.
PB  - Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki
C3  - TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece
T1  - In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds
SP  - 132
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6320
ER  - 

@conference{
author = "Skorić, Marijana and Todorović, Slađana and Ristić, Mihailo and Soković, Marina and Glamočlija, Jasmina and Živković, Suzana and Stojić, Andreja and Puač, Nevena and Kanellis, Angelos K",
year = "2013",
abstract = "Cistus creticus subsp. creticus is a native plant of the Mediterranean region and it has been used since ancient times for its medicinal properties. Labdane type diterpenes are predominant compounds in leaves and stems of the C. creticus subsp. creticus, as well as in resin, while other terpenes, polyphenols and flavonoids contribute to chemical profile of this species. In vitro plant tissue culture is an attractive alternative approach to the traditional methods of plantations, as it offers a controlled supply of biochemicals independent of plant availability and more consistent product quality. In order to obtain applicable production of secondary metabolites of interest we have implemented in vitro culture of several clones of C. creticus, which were previously morphogenetically described. The ethanol extracts of obtained in vitro cultures were analyzed by GC-FID and GC-MS, and were characterized concerning the total phenolic and flavonoid contents. Labdane diterpenes were the most abundant compounds in above ground parts extracts, but absent from the root extracts. Head-space GC-MS was used to determine the profile of volatile compounds in the atmosphere of the culture vessels. α pinene, β pinene, and camphene were the most abundant ones. PTR-MS was used for further quantification of representative volatile compounds in the atmosphere of the culture vessels. Finally, the biological activity of obtained ethanol extract was studied. Microdilution method was used for investigation of antibacterial and antifungal activity. Almost all tested extract against human pathogenic bacteria showed better antibacterial activity than streptomycin, and much better antibacterial activity than ampicillin. All tested extracts also exhibited slightly higher or similar antifungal potential as bifonazole, and showed much better antifungal effect than ketoconazole. Antioxidant activity was determined by using DPPH (2,2-Diphenyl-1-picrylhydrazyl) test, while cytotoxic activity was previously demonstrated by sulforhodamine B (SRB) assay.",
publisher = "Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki",
journal = "TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece",
title = "In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds",
pages = "132",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6320"
}

Skorić, M., Todorović, S., Ristić, M., Soković, M., Glamočlija, J., Živković, S., Stojić, A., Puač, N.,& Kanellis, A. K.. (2013). In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds. in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece
Thessaloniki: Department of Pharmaceutical Science, Aristotle University of Thessaloniki., 132.
https://hdl.handle.net/21.15107/rcub_ibiss_6320

Skorić M, Todorović S, Ristić M, Soković M, Glamočlija J, Živković S, Stojić A, Puač N, Kanellis AK. In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds. in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece. 2013;:132.
https://hdl.handle.net/21.15107/rcub_ibiss_6320 .

Skorić, Marijana, Todorović, Slađana, Ristić, Mihailo, Soković, Marina, Glamočlija, Jasmina, Živković, Suzana, Stojić, Andreja, Puač, Nevena, Kanellis, Angelos K, "In vitro culture of Cistus creticus subsp. creticus – a source of biological active compounds" in TERPNET 2013: 11th International meeting: Biosynthesis, Function and Biotechnology of Isoprenoids in Terrestrial and Marine Organisms; 2013 Jun 1-5; Kolymvari, Greece (2013):132,
https://hdl.handle.net/21.15107/rcub_ibiss_6320 .