@conference{
author = "Jovanović Stojanov, Sofija and Podolski-Renić, Ana and Dinić, Jelena and Stojkovska, Jasmina and Obradović, Bojana and Pešić, Milica and Dragoj, Miodrag",
year = "2024",
abstract = "In our study, we aimed to develop a long-term three-dimensional (3D) model for glioblastoma cell culture to facilitate more reliable drug response studies. We cultured human U87 glioblastoma cells in alginate microfibers for a period of 28 days. Throughout this time, we monitored cell growth, viability, morphology, and aggregation in the 3D culture using fluorescent and confocal microscopy. Calcein-AM/propidium iodide staining was performed every seven days.
We validated the glioblastoma 3D model by subjecting the cells to temozolomide (TMZ) treatments for three consecutive days, starting from the 7th day of culturing cells in alginate microfibers. After a recovery period of 18 days, we evaluated cell viability using MTT assays and assessed the expression of resistance-related genes (MGMT and ABCB1) using qPCR. We also applied the same TMZ treatment schedule to cells cultured in a two-dimensional (2D) setting for comparison purposes.
Our results showed that within the long-term 3D model system in alginate fibers, the U87 cells remained viable for the entire 28-day period. Furthermore, on day 7, we observed that the cells formed visible aggregates oriented towards the periphery of the microfibers. Upon TMZ treatment, we observed a reduction in cell growth, as well as an increase in the expression of drug resistance-related genes. This effect was more pronounced in the 3D culture compared to the 2D culture.
In conclusion, we established a novel glioblastoma 3D model system, which could be particularly valuable for conducting long-term drug testing and optimizing treatment strategies.",
publisher = "Ljubljana: National Institute of Biology",
journal = "Book of Abstracts: 1st Net4Brain Annual Meeting: Closing the translational gap in brain cancer treatment; 2024 Sep 4-6; Ljubljana, Slovenia",
title = "3D Glioblastoma Cell Culture within Alginate Microfibers for Long-Term Evaluation of Drug Effects",
pages = "28",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_7012"
}