TY  - JOUR
AU  - Vidonja Uzelac, Teodora
AU  - Tatalović, Nikola
AU  - Mijović, Milica
AU  - Koželj, Gordana
AU  - Nikolić-Kokić, Aleksandra
AU  - Oreščanin Dušić, Zorana
AU  - Bresjanac, Mara
AU  - Blagojević, Duško
PY  - 2019
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0354-46641800055V
UR  - http://www.serbiosoc.org.rs/arch/index.php/abs/article/view/3420
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3353
AB  - Ibogaine, administered as a single oral dose (1-25 mg/kg body weight), has been used as an addiction-interrupting agent. Its effects persist for up to 72 h. Ex vivo results showed that ibogaine induced cellular energy consumption and restitution, followed by increased reactive oxygen species production and antioxidant activity. Therefore, the aim of this work was to explore the effect of a single oral dose of ibogaine (1 or 20 mg/kg body weight) on antioxidative defenses in rat liver and erythrocytes. Six and 24 h after ibogaine administration, histological examination showed glycogenolytic activity in hepatocytes, which was highest after 24 h in animals that received 20 mg/kg ibogaine. There were no changes in the activities of superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase in the liver and erythrocytes after ibogaine treatment, regardless of the dose. Hepatic xanthine oxidase activity was elevated in rats that received 20 mg/kg compared to the controls (p<0.01), suggesting faster adenosine turnover. TBARS concentration was elevated in the group treated with 1 mg/kg after 24 h compared to the controls (p<0.01), suggesting mild oxidative stress. Our results show that ibogaine treatment influenced hepatic redox homeostasis, but not sufficiently to remodel antioxidant enzyme activities at 6 and 24 h post-ibogaine application.
T2  - Archives of Biological Sciences
T1  - Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes
IS  - 1
VL  - 71
DO  - 10.2298/ABS180918055V
SP  - 133
EP  - 144
ER  - 

@article{
author = "Vidonja Uzelac, Teodora and Tatalović, Nikola and Mijović, Milica and Koželj, Gordana and Nikolić-Kokić, Aleksandra and Oreščanin Dušić, Zorana and Bresjanac, Mara and Blagojević, Duško",
year = "2019",
abstract = "Ibogaine, administered as a single oral dose (1-25 mg/kg body weight), has been used as an addiction-interrupting agent. Its effects persist for up to 72 h. Ex vivo results showed that ibogaine induced cellular energy consumption and restitution, followed by increased reactive oxygen species production and antioxidant activity. Therefore, the aim of this work was to explore the effect of a single oral dose of ibogaine (1 or 20 mg/kg body weight) on antioxidative defenses in rat liver and erythrocytes. Six and 24 h after ibogaine administration, histological examination showed glycogenolytic activity in hepatocytes, which was highest after 24 h in animals that received 20 mg/kg ibogaine. There were no changes in the activities of superoxide dismutases, catalase, glutathione peroxidase, glutathione reductase and glutathione-S-transferase in the liver and erythrocytes after ibogaine treatment, regardless of the dose. Hepatic xanthine oxidase activity was elevated in rats that received 20 mg/kg compared to the controls (p<0.01), suggesting faster adenosine turnover. TBARS concentration was elevated in the group treated with 1 mg/kg after 24 h compared to the controls (p<0.01), suggesting mild oxidative stress. Our results show that ibogaine treatment influenced hepatic redox homeostasis, but not sufficiently to remodel antioxidant enzyme activities at 6 and 24 h post-ibogaine application.",
journal = "Archives of Biological Sciences",
title = "Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes",
number = "1",
volume = "71",
doi = "10.2298/ABS180918055V",
pages = "133-144"
}

Vidonja Uzelac, T., Tatalović, N., Mijović, M., Koželj, G., Nikolić-Kokić, A., Oreščanin Dušić, Z., Bresjanac, M.,& Blagojević, D.. (2019). Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes. in Archives of Biological Sciences, 71(1), 133-144.
https://doi.org/10.2298/ABS180918055V

Vidonja Uzelac T, Tatalović N, Mijović M, Koželj G, Nikolić-Kokić A, Oreščanin Dušić Z, Bresjanac M, Blagojević D. Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes. in Archives of Biological Sciences. 2019;71(1):133-144.
doi:10.2298/ABS180918055V .

Vidonja Uzelac, Teodora, Tatalović, Nikola, Mijović, Milica, Koželj, Gordana, Nikolić-Kokić, Aleksandra, Oreščanin Dušić, Zorana, Bresjanac, Mara, Blagojević, Duško, "Effects of ibogaine per os application on redox homeostasis in rat liver and erythrocytes" in Archives of Biological Sciences, 71, no. 1 (2019):133-144,
https://doi.org/10.2298/ABS180918055V . .

TY  - JOUR
AU  - Paskulin, Roman
AU  - Jamnik, Polona
AU  - Danevcić, Tjasa
AU  - Kozelj, Gordana
AU  - Krasovec, Rok
AU  - Krstić Milošević, Dijana
AU  - Blagojević, Duško
AU  - Strukelj, Borut
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1149
AB  - Ethnopharmacological relevance: The root bark of iboga plant-Tabernanthe iboga has been used traditionally in Central Africa as a psychoactive substance in religious rituals, while in smaller doses it is appreciated due to its stimulant properties. The iboga root bark, iboga extract or pure ibogaine are being recognized in the West as an anti-addiction remedy and their use is increasing. Aim of the study: Our previous studies have demonstrated a transient ATP pool reduction under ibogaine accompanied by the induction of energy metabolism related enzymes. The present study aimed to find the cause of this energy deprivation and to foresee its immediate and long-term impact on metabolism. The overall project is designed to disclose the common mechanism of action at these seemingly diverse indications for iboga use, to predict eventual adverse effects and to build the grounds for its safe and beneficial utilization. Materials and methods: The rate of carbon dioxide (CO2) as a marker of energy metabolism in stationary yeast model under aerobic conditions in the presence of ibogaine at concentration of 1, 4 and 20 mg/l was measured for 5 h by gas chromatography. The overall oxidative load was determined fluorimetrically by 2',7'-dichlorofluorescein diacetate (H(2)DCFDA) and in vitro antioxidant properties of ibogaine were defined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. Results: The CO2 production under ibogaine was temporarily increased in a dose dependent manner. The increased energy consumption as an early effect of ibogaine was proven by the fact that in spite of energy mobilization, the ATP pool has been simultaneously decreased. Although increased cellular respiration co-produces reactive oxygen species (ROS), the overall oxidative load was significantly lowered by ibogaine. Since ibogaine does not show any significant in vitro antioxidant properties, the results indicate its stimulating influence on physiological oxidative stress defence system. Conclusion: Ibogaine triggers remodeling of the housekeeping metabolism. Under the initial energy cost it results in increased efficacy of physiological antioxidative systems, which reduce oxidative damage and lowers basal metabolic needs. Together with induced catabolic enzymes they set a new metabolic equilibrium that saves energy and makes it easily available in case of extra needs. While healthy organism profits from improved fitness and mental performance and can withstand higher stress without risking a disease, due to the same principle ibogaine provides beneficial support at the recovery after diseases including addiction syndrome. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
T2  - Journal of Ethnopharmacology
T1  - Metabolic plasticity and the energy economizing effect of ibogaine, the principal alkaloid of Tabernanthe iboga
IS  - 1
VL  - 143
SP  - 1035
EP  - 324
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1149
ER  - 

@article{
author = "Paskulin, Roman and Jamnik, Polona and Danevcić, Tjasa and Kozelj, Gordana and Krasovec, Rok and Krstić Milošević, Dijana and Blagojević, Duško and Strukelj, Borut",
year = "2012",
abstract = "Ethnopharmacological relevance: The root bark of iboga plant-Tabernanthe iboga has been used traditionally in Central Africa as a psychoactive substance in religious rituals, while in smaller doses it is appreciated due to its stimulant properties. The iboga root bark, iboga extract or pure ibogaine are being recognized in the West as an anti-addiction remedy and their use is increasing. Aim of the study: Our previous studies have demonstrated a transient ATP pool reduction under ibogaine accompanied by the induction of energy metabolism related enzymes. The present study aimed to find the cause of this energy deprivation and to foresee its immediate and long-term impact on metabolism. The overall project is designed to disclose the common mechanism of action at these seemingly diverse indications for iboga use, to predict eventual adverse effects and to build the grounds for its safe and beneficial utilization. Materials and methods: The rate of carbon dioxide (CO2) as a marker of energy metabolism in stationary yeast model under aerobic conditions in the presence of ibogaine at concentration of 1, 4 and 20 mg/l was measured for 5 h by gas chromatography. The overall oxidative load was determined fluorimetrically by 2',7'-dichlorofluorescein diacetate (H(2)DCFDA) and in vitro antioxidant properties of ibogaine were defined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. Results: The CO2 production under ibogaine was temporarily increased in a dose dependent manner. The increased energy consumption as an early effect of ibogaine was proven by the fact that in spite of energy mobilization, the ATP pool has been simultaneously decreased. Although increased cellular respiration co-produces reactive oxygen species (ROS), the overall oxidative load was significantly lowered by ibogaine. Since ibogaine does not show any significant in vitro antioxidant properties, the results indicate its stimulating influence on physiological oxidative stress defence system. Conclusion: Ibogaine triggers remodeling of the housekeeping metabolism. Under the initial energy cost it results in increased efficacy of physiological antioxidative systems, which reduce oxidative damage and lowers basal metabolic needs. Together with induced catabolic enzymes they set a new metabolic equilibrium that saves energy and makes it easily available in case of extra needs. While healthy organism profits from improved fitness and mental performance and can withstand higher stress without risking a disease, due to the same principle ibogaine provides beneficial support at the recovery after diseases including addiction syndrome. (C) 2012 Elsevier Ireland Ltd. All rights reserved.",
journal = "Journal of Ethnopharmacology",
title = "Metabolic plasticity and the energy economizing effect of ibogaine, the principal alkaloid of Tabernanthe iboga",
number = "1",
volume = "143",
pages = "1035-324",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1149"
}

Paskulin, R., Jamnik, P., Danevcić, T., Kozelj, G., Krasovec, R., Krstić Milošević, D., Blagojević, D.,& Strukelj, B.. (2012). Metabolic plasticity and the energy economizing effect of ibogaine, the principal alkaloid of Tabernanthe iboga. in Journal of Ethnopharmacology, 143(1), 1035-324.
https://hdl.handle.net/21.15107/rcub_ibiss_1149

Paskulin R, Jamnik P, Danevcić T, Kozelj G, Krasovec R, Krstić Milošević D, Blagojević D, Strukelj B. Metabolic plasticity and the energy economizing effect of ibogaine, the principal alkaloid of Tabernanthe iboga. in Journal of Ethnopharmacology. 2012;143(1):1035-324.
https://hdl.handle.net/21.15107/rcub_ibiss_1149 .

Paskulin, Roman, Jamnik, Polona, Danevcić, Tjasa, Kozelj, Gordana, Krasovec, Rok, Krstić Milošević, Dijana, Blagojević, Duško, Strukelj, Borut, "Metabolic plasticity and the energy economizing effect of ibogaine, the principal alkaloid of Tabernanthe iboga" in Journal of Ethnopharmacology, 143, no. 1 (2012):1035-324,
https://hdl.handle.net/21.15107/rcub_ibiss_1149 .