TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5827
AB  - In this study, the effects of foliar applied methyl jasmonate (MeJA) on drought-stressed Impatiens walleriana growth and leaf physiology parameters: stomatal conductance, chlorophyll, flavonoid, anthocyanin, and nitrogen balance index (NBI), were evaluated. These parameters could serve as indicators of drought tolerance of I. walleriana, a popular horticultural plant worldwide that is very sensitive to drought. The experiment included four treatments: control, drought-stressed plants sprayed with distilled water, drought-stressed plants sprayed with 5 µM MeJA, and drought-stressed plants sprayed with 50 µM MeJA. Foliar spraying with MeJA was performed twice: seven days before and on the day of drought induction. The stressed plant groups were non-irrigated to achieve soil water contents (SWC) of 15 and 5%, while control plants were well-watered throughout the experiment (35–37% SWC). The results of this study showed that drought significantly reduced I. walleriana fresh and dry shoot weight, as well as total leaf area, but did not impact on dry matter content. The foliar application of MeJA improved growth parameters of I. walleriana, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC, and foliar applied MeJA at both concentrations. The flavonoid index was slightly reduced at 15 and 5% SWC when 50 µM MeJA was foliar applied, while there were no observed changes in the anthocyanin index in any treatments. The foliar application of 50 µM MeJA increased the chlorophyll index and NBI of I. walleriana at 5% SWC, indicating a contribution of the elicitor to plant drought tolerance at the physiological level.
PB  - Taylor & Francis Group
T2  - Plant Signaling & Behavior
T1  - Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress
IS  - 1
VL  - 18
DO  - 10.1080/15592324.2023.2219936
SP  - e2219936
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "In this study, the effects of foliar applied methyl jasmonate (MeJA) on drought-stressed Impatiens walleriana growth and leaf physiology parameters: stomatal conductance, chlorophyll, flavonoid, anthocyanin, and nitrogen balance index (NBI), were evaluated. These parameters could serve as indicators of drought tolerance of I. walleriana, a popular horticultural plant worldwide that is very sensitive to drought. The experiment included four treatments: control, drought-stressed plants sprayed with distilled water, drought-stressed plants sprayed with 5 µM MeJA, and drought-stressed plants sprayed with 50 µM MeJA. Foliar spraying with MeJA was performed twice: seven days before and on the day of drought induction. The stressed plant groups were non-irrigated to achieve soil water contents (SWC) of 15 and 5%, while control plants were well-watered throughout the experiment (35–37% SWC). The results of this study showed that drought significantly reduced I. walleriana fresh and dry shoot weight, as well as total leaf area, but did not impact on dry matter content. The foliar application of MeJA improved growth parameters of I. walleriana, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC, and foliar applied MeJA at both concentrations. The flavonoid index was slightly reduced at 15 and 5% SWC when 50 µM MeJA was foliar applied, while there were no observed changes in the anthocyanin index in any treatments. The foliar application of 50 µM MeJA increased the chlorophyll index and NBI of I. walleriana at 5% SWC, indicating a contribution of the elicitor to plant drought tolerance at the physiological level.",
publisher = "Taylor & Francis Group",
journal = "Plant Signaling & Behavior",
title = "Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress",
number = "1",
volume = "18",
doi = "10.1080/15592324.2023.2219936",
pages = "e2219936"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress. in Plant Signaling & Behavior
Taylor & Francis Group., 18(1), e2219936.
https://doi.org/10.1080/15592324.2023.2219936

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress. in Plant Signaling & Behavior. 2023;18(1):e2219936.
doi:10.1080/15592324.2023.2219936 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Foliar application of methyl jasmonate affects impatiens walleriana growth and leaf physiology under drought stress" in Plant Signaling & Behavior, 18, no. 1 (2023):e2219936,
https://doi.org/10.1080/15592324.2023.2219936 . .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5762
AB  - Drought stress affects plant growth and development through several mechanisms, including the induction of oxidative stress. To cope with drought, plants have drought tolerance mechanisms at the physiological, biochemical, and molecular levels. In this study, the effects of foliar application of distilled water and methyl jasmonate (MeJA) (5 and 50 µM) on the physiological, biochemical, and molecular responses of Impatiens walleriana during two drought regimes (15 and 5% soil water content, SWC) were investigated. The results showed that plant response depended on the concentration of the elicitor and the stress intensity. The highest chlorophyll and carotenoid contents were observed at 5% SWC in plants pre-treated with 50 µM MeJA, while the MeJA did not have a significant effect on the chlorophyll a/b ratio in drought-stressed plants. Drought-induced formation of hydrogen peroxide and malondialdehyde in plants sprayed with distilled water was significantly reduced in plant leaves pretreated with MeJA. The lower total polyphenol content and antioxidant activity of secondary metabolites in MeJA-pretreated plants were observed. The foliar application of MeJA affected the proline content and antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase) in plants that suffered from drought. The expression of abscisic acid (ABA) metabolic genes (IwNCED4, IwAAO2, and IwABA8ox3) was the most affected in plants sprayed with 50 µM MeJA, while of the four analyzed aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7, and IwTIP4;1), the expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants pre-treated with 50 µM MeJA. The study’s findings demonstrated the significance of MeJA in regulating the gene expression of the ABA metabolic pathway and aquaporins, as well as the considerable alterations in oxidative stress responses of drought-stressed I. walleriana foliar sprayed with MeJA. The results improved our understanding of this horticulture plant’s stress physiology and the field of plant hormones’ interaction network in general.
PB  - Basel: MDPI
T2  - Genes
T1  - Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application
IS  - 5
VL  - 14
DO  - 10.3390/genes14051072
SP  - 1072
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "Drought stress affects plant growth and development through several mechanisms, including the induction of oxidative stress. To cope with drought, plants have drought tolerance mechanisms at the physiological, biochemical, and molecular levels. In this study, the effects of foliar application of distilled water and methyl jasmonate (MeJA) (5 and 50 µM) on the physiological, biochemical, and molecular responses of Impatiens walleriana during two drought regimes (15 and 5% soil water content, SWC) were investigated. The results showed that plant response depended on the concentration of the elicitor and the stress intensity. The highest chlorophyll and carotenoid contents were observed at 5% SWC in plants pre-treated with 50 µM MeJA, while the MeJA did not have a significant effect on the chlorophyll a/b ratio in drought-stressed plants. Drought-induced formation of hydrogen peroxide and malondialdehyde in plants sprayed with distilled water was significantly reduced in plant leaves pretreated with MeJA. The lower total polyphenol content and antioxidant activity of secondary metabolites in MeJA-pretreated plants were observed. The foliar application of MeJA affected the proline content and antioxidant enzyme activities (superoxide dismutase, peroxidase, and catalase) in plants that suffered from drought. The expression of abscisic acid (ABA) metabolic genes (IwNCED4, IwAAO2, and IwABA8ox3) was the most affected in plants sprayed with 50 µM MeJA, while of the four analyzed aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7, and IwTIP4;1), the expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants pre-treated with 50 µM MeJA. The study’s findings demonstrated the significance of MeJA in regulating the gene expression of the ABA metabolic pathway and aquaporins, as well as the considerable alterations in oxidative stress responses of drought-stressed I. walleriana foliar sprayed with MeJA. The results improved our understanding of this horticulture plant’s stress physiology and the field of plant hormones’ interaction network in general.",
publisher = "Basel: MDPI",
journal = "Genes",
title = "Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application",
number = "5",
volume = "14",
doi = "10.3390/genes14051072",
pages = "1072"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application. in Genes
Basel: MDPI., 14(5), 1072.
https://doi.org/10.3390/genes14051072

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application. in Genes. 2023;14(5):1072.
doi:10.3390/genes14051072 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Alterations in Physiological, Biochemical, and Molecular Responses of Impatiens walleriana to Drought by Methyl Jasmonate Foliar Application" in Genes, 14, no. 5 (2023):1072,
https://doi.org/10.3390/genes14051072 . .

TY  - CONF
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5763
AB  - This research evaluated the effects of foliarly applied elicitor methyl jasmonate (MeJA) on the potential improvement of drought tolerance in Impatiens walleriana. Growth and leaf physiology parameters (Stomatal conductance, Chlorophyll, Flavonoid, Anthocyanin, and Nitrogen Balance Index - NBI), as well the expression of four aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1), were evaluated. These parameters could serve as indicators of drought tolerance in I. walleriana, a worldwide popular horticultural plant, very sensitive to drought. The experiment included four treatments: control plants, drought-stressed plants foliarly sprayed with distilled water, drought-stressed plants foliarly sprayed with 5 μM MeJA, and drought-stressed plants foliarly sprayed with 50 μM MeJA. Foliar spraying was performed seven days before drought induction, and on the day of drought stress induction. The stressed plant groups were non-irrigated to reach 15 and 5% soil water content (SWC), while control plants were well-watered (35-37% SWC) during the entire experiment. Drought reduced fresh and dry shoot weight, as well total leaf area but dry matter content was not affected. Foliarly applied MeJA improveda growth parameters, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC in plants foliarly sprayed with MeJA regardless of the used concentration. Similarly, the flavonoid index was reduced at 15 and 5% SWC when 50 μM MeJA was foliarly applied, while there were no observed changes in the anthocyanin index in any treatment. The foliar application of 50 μM MeJA increased I. walleriana chlorophyll index and NBI at 5% SWC, indicating an elicitor contribution to plant drought tolerance at physiological level. Among the four analyzed aquaporin genes, expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants foliarly pre-treated with 50 μM MeJA, indicating an improvement of water flow through cells to maintain homeostasis.
PB  - Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo
C3  - Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina
T1  - Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana
SP  - 141
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5763
ER  - 

@conference{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2023",
abstract = "This research evaluated the effects of foliarly applied elicitor methyl jasmonate (MeJA) on the potential improvement of drought tolerance in Impatiens walleriana. Growth and leaf physiology parameters (Stomatal conductance, Chlorophyll, Flavonoid, Anthocyanin, and Nitrogen Balance Index - NBI), as well the expression of four aquaporin genes (IwPIP1;4, IwPIP2;2, IwPIP2;7 and IwTIP4;1), were evaluated. These parameters could serve as indicators of drought tolerance in I. walleriana, a worldwide popular horticultural plant, very sensitive to drought. The experiment included four treatments: control plants, drought-stressed plants foliarly sprayed with distilled water, drought-stressed plants foliarly sprayed with 5 μM MeJA, and drought-stressed plants foliarly sprayed with 50 μM MeJA. Foliar spraying was performed seven days before drought induction, and on the day of drought stress induction. The stressed plant groups were non-irrigated to reach 15 and 5% soil water content (SWC), while control plants were well-watered (35-37% SWC) during the entire experiment. Drought reduced fresh and dry shoot weight, as well total leaf area but dry matter content was not affected. Foliarly applied MeJA improveda growth parameters, depending on the elicitor concentration and drought intensity. Stomatal conductance was slightly reduced at 5% SWC in plants foliarly sprayed with MeJA regardless of the used concentration. Similarly, the flavonoid index was reduced at 15 and 5% SWC when 50 μM MeJA was foliarly applied, while there were no observed changes in the anthocyanin index in any treatment. The foliar application of 50 μM MeJA increased I. walleriana chlorophyll index and NBI at 5% SWC, indicating an elicitor contribution to plant drought tolerance at physiological level. Among the four analyzed aquaporin genes, expression of IwPIP1;4 and IwPIP2;7 was strongly induced in drought-stressed plants foliarly pre-treated with 50 μM MeJA, indicating an improvement of water flow through cells to maintain homeostasis.",
publisher = "Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo",
journal = "Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina",
title = "Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana",
pages = "141",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5763"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2023). Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana. in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina
Sarajevo: Institute for Genetic Engineering and Biotechnology, University of Sarajevo., 141.
https://hdl.handle.net/21.15107/rcub_ibiss_5763

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana. in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina. 2023;:141.
https://hdl.handle.net/21.15107/rcub_ibiss_5763 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Foliar application of methyl jasmonate affected growth, leaf physiology parameters and aquaporin genes expression in drought-stressed Impatiens walleriana" in Book of abstracts: International Conference of Biochemists and Molecular Biologists in Bosnia and Herzegovina - ABMBBIH; 2023 May 18-23; Sarajevo, Bosnia and Herzegovina (2023):141,
https://hdl.handle.net/21.15107/rcub_ibiss_5763 .

TY  - CONF
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Milošević, Snežana
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5363
AB  - Суша представља ограничавајући фактор у производњи комерцијално значајних
биљних врста, испољавајући негативне ефекте у свим развојним стадијумима. У
овом истраживању испитиван је утицај фолијарне примене елицитора метил
јасмоната (МеЈА) на растење и физиолошки одговор Лепог Јове (Impatiens
walleriana) при суши. Лепи Јова се убраја у најпопуларније хортикултурне врсте
широм света, а ограничавајући фактор у производњи, транспорту и пласману
биљака на тржишту представља дефицит воде. Елицитација подразумева примену
једињења у циљу индуковања сигналних путева повезаних са одбраном биљака од
стреса, а у ту сврху често се користе регулатори растења попут МеЈА. Фолијарно
третирање биљака МеЈА (5 и 50 μМ) вршено је два пута током експерименталног
рада: седам дана пре индуковања суше и на дан индукције суше. Биљке су
подељене у групе према третманима, а суша је индукована тако што биљке нису
заливане до достизања жељених процената влажности супстрата (15 и 5%). Оба
третмана суше укључивала су, поред контролних биљака (35-37% влажности
супстрата) и биљке које су постепено рехидратисане – заливане четири дана након
исушивања до достизања контролних вредности влажности супстрата. Забележен
је позитиван утицај МеЈА на параметре растења: масу свежих и сувих изданака,
као и укупну површину листова Лепог Јове. Код група биљака третираних МеЈА,
забележен је пад у концентрацији показатеља оксидативног стреса –
малондиалдехида и H2О2 при суши, као и мањи садржај полифенола и флавонола.
AB  - Suša predstavlja ograničavajući faktor u proizvodnji komercijalno značajnih biljnih vrsta, ispoljavajući negativne efekte u svim razvojnim stadijumima. U ovom istraživanju ispitivan je uticaj folijarne primene elicitora metil jasmonata (MeJA) na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri suši. Lepi Jova se ubraja u najpopularnije hortikulturne vrste širom sveta, a ograničavajući faktor u proizvodnji, transportu i plasmanu biljaka na tržištu predstavlja deficit vode. Elicitacija podrazumeva primenu jedinjenja u cilju indukovanja signalnih puteva povezanih sa odbranom biljaka od stresa, a u tu svrhu često se koriste regulatori rastenja poput MeJA. Folijarno tretiranje biljaka MeJA (5 i 50 μM) vršeno je dva puta tokom eksperimentalnog rada: sedam dana pre indukovanja suše i na dan indukcije suše. Biljke su podeljene u grupe prema tretmanima, a suša je indukovana tako što biljke nisu zalivane do dostizanja željenih procenata vlažnosti supstrata (15 i 5%). Oba tretmana suše uključivala su, pored kontrolnih biljaka (35-37% vlažnosti supstrata) i biljke koje su postepeno rehidratisane – zalivane četiri dana nakon isušivanja do dostizanja kontrolnih vrednosti vlažnosti supstrata. Zabeležen je pozitivan uticaj MeJA na parametre rastenja: masu svežih i suvih izdanaka, kao i ukupnu površinu listova Lepog Jove. Kod grupa biljaka tretiranih MeJA, zabeležen je pad u koncentraciji pokazatelja oksidativnog stresa – malondialdehida i H2O2 pri suši, kao i manji sadržaj polifenola i flavonola.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши
T1  - Uticaj folijarne primene metil jasmonata na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri indukovanoj suši
IS  - 048
VL  - 57
SP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5363
ER  - 

@conference{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Milošević, Snežana",
year = "2022",
abstract = "Суша представља ограничавајући фактор у производњи комерцијално значајних
биљних врста, испољавајући негативне ефекте у свим развојним стадијумима. У
овом истраживању испитиван је утицај фолијарне примене елицитора метил
јасмоната (МеЈА) на растење и физиолошки одговор Лепог Јове (Impatiens
walleriana) при суши. Лепи Јова се убраја у најпопуларније хортикултурне врсте
широм света, а ограничавајући фактор у производњи, транспорту и пласману
биљака на тржишту представља дефицит воде. Елицитација подразумева примену
једињења у циљу индуковања сигналних путева повезаних са одбраном биљака од
стреса, а у ту сврху често се користе регулатори растења попут МеЈА. Фолијарно
третирање биљака МеЈА (5 и 50 μМ) вршено је два пута током експерименталног
рада: седам дана пре индуковања суше и на дан индукције суше. Биљке су
подељене у групе према третманима, а суша је индукована тако што биљке нису
заливане до достизања жељених процената влажности супстрата (15 и 5%). Оба
третмана суше укључивала су, поред контролних биљака (35-37% влажности
супстрата) и биљке које су постепено рехидратисане – заливане четири дана након
исушивања до достизања контролних вредности влажности супстрата. Забележен
је позитиван утицај МеЈА на параметре растења: масу свежих и сувих изданака,
као и укупну површину листова Лепог Јове. Код група биљака третираних МеЈА,
забележен је пад у концентрацији показатеља оксидативног стреса –
малондиалдехида и H2О2 при суши, као и мањи садржај полифенола и флавонола., Suša predstavlja ograničavajući faktor u proizvodnji komercijalno značajnih biljnih vrsta, ispoljavajući negativne efekte u svim razvojnim stadijumima. U ovom istraživanju ispitivan je uticaj folijarne primene elicitora metil jasmonata (MeJA) na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri suši. Lepi Jova se ubraja u najpopularnije hortikulturne vrste širom sveta, a ograničavajući faktor u proizvodnji, transportu i plasmanu biljaka na tržištu predstavlja deficit vode. Elicitacija podrazumeva primenu jedinjenja u cilju indukovanja signalnih puteva povezanih sa odbranom biljaka od stresa, a u tu svrhu često se koriste regulatori rastenja poput MeJA. Folijarno tretiranje biljaka MeJA (5 i 50 μM) vršeno je dva puta tokom eksperimentalnog rada: sedam dana pre indukovanja suše i na dan indukcije suše. Biljke su podeljene u grupe prema tretmanima, a suša je indukovana tako što biljke nisu zalivane do dostizanja željenih procenata vlažnosti supstrata (15 i 5%). Oba tretmana suše uključivala su, pored kontrolnih biljaka (35-37% vlažnosti supstrata) i biljke koje su postepeno rehidratisane – zalivane četiri dana nakon isušivanja do dostizanja kontrolnih vrednosti vlažnosti supstrata. Zabeležen je pozitivan uticaj MeJA na parametre rastenja: masu svežih i suvih izdanaka, kao i ukupnu površinu listova Lepog Jove. Kod grupa biljaka tretiranih MeJA, zabeležen je pad u koncentraciji pokazatelja oksidativnog stresa – malondialdehida i H2O2 pri suši, kao i manji sadržaj polifenola i flavonola.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши, Uticaj folijarne primene metil jasmonata na rastenje i fiziološki odgovor Lepog Jove (Impatiens walleriana) pri indukovanoj suši",
number = "048",
volume = "57",
pages = "64",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5363"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M.,& Milošević, S.. (2022). Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 57(048), 64.
https://hdl.handle.net/21.15107/rcub_ibiss_5363

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Milošević S. Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;57(048):64.
https://hdl.handle.net/21.15107/rcub_ibiss_5363 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Milošević, Snežana, "Утицај фолијарне примене метил јасмоната на растење и физиолошки одговор Лепог Јове (Impatiens walleriana) при индукованој суши" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia, 57, no. 048 (2022):64,
https://hdl.handle.net/21.15107/rcub_ibiss_5363 .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Dragićević, Milan
AU  - Simonović, Ana
AU  - Milošević, Snežana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4119
AB  - Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.
PB  - Basel, Switzerland: MDPI
T2  - Plants
T1  - Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery
IS  - 1
VL  - 10
DO  - 10.3390/plants10010154
SP  - 154
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Dragićević, Milan and Simonović, Ana and Milošević, Snežana",
year = "2021",
abstract = "Aquaporins comprise a large group of transmembrane proteins responsible for water
transport, which is crucial for plant survival under stress conditions. Despite the vital role of aquaporins,
nothing is known about this protein family in Impatiens walleriana, a commercially important
horticultural plant, which is sensitive to drought stress. In the present study, attention is given to
the molecular characterization of aquaporins in I. walleriana and their expression during drought
stress and recovery. We identified four I. walleriana aquaporins: IwPIP1;4, IwPIP2;2, IwPIP2;7 and
IwTIP4;1. All of them had conserved NPA motifs (Asparagine-Proline-Alanine), transmembrane
helices (TMh), pore characteristics, stereochemical properties and tetrameric structure of holoprotein.
Drought stress and recovery treatment affected the aquaporins expression in I. walleriana
leaves, which was up- or downregulated depending on stress intensity. Expression of IwPIP2;7 was
the most affected of all analyzed I. walleriana aquaporins. At 15% and 5% soil moisture and recovery
from 15% and 5% soil moisture, IwPIP2;7 expression significantly decreased and increased, respectively.
Aquaporins IwPIP1;4 and IwTIP4;1 had lower expression in comparison to IwPIP2;7, with
moderate expression changes in response to drought and recovery, while IwPIP2;2 expression was
of significance only in recovered plants. Insight into the molecular structure of I. walleriana aquaporins
expanded knowledge about plant aquaporins, while its expression during drought and recovery
contributed to I. walleriana drought tolerance mechanisms and re-acclimation.",
publisher = "Basel, Switzerland: MDPI",
journal = "Plants",
title = "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery",
number = "1",
volume = "10",
doi = "10.3390/plants10010154",
pages = "154"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M., Cingel, A., Dragićević, M., Simonović, A.,& Milošević, S.. (2021). Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants
Basel, Switzerland: MDPI., 10(1), 154.
https://doi.org/10.3390/plants10010154

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Cingel A, Dragićević M, Simonović A, Milošević S. Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery. in Plants. 2021;10(1):154.
doi:10.3390/plants10010154 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Dragićević, Milan, Simonović, Ana, Milošević, Snežana, "Molecular characterization and expression of four aquaporin genes in Impatiens walleriana during drought stress and recovery" in Plants, 10, no. 1 (2021):154,
https://doi.org/10.3390/plants10010154 . .

TY  - JOUR
AU  - Kolarević, Tijana
AU  - Milinčić, Danijel D.
AU  - Vujović, Tatjana
AU  - Gašić, Uroš
AU  - Prokić, Ljiljana
AU  - Kostić, Aleksandar Ž.
AU  - Cerović, Radosav
AU  - Stanojević, Slađana P.
AU  - Tešić, Živoslav Lj.
AU  - Pešić, Mirjana B.
PY  - 2021
UR  - https://www.mdpi.com/2311-7524/7/11/420
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4665
AB  - The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to ½, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.
PB  - Basel: MDPI
T2  - Horticulturae
T1  - Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry
IS  - 11
VL  - 7
DO  - 10.3390/horticulturae7110420
SP  - 420
ER  - 

@article{
author = "Kolarević, Tijana and Milinčić, Danijel D. and Vujović, Tatjana and Gašić, Uroš and Prokić, Ljiljana and Kostić, Aleksandar Ž. and Cerović, Radosav and Stanojević, Slađana P. and Tešić, Živoslav Lj. and Pešić, Mirjana B.",
year = "2021",
abstract = "The aim of this study was to evaluate the content and profile of the phenolic compounds (PCs) and antioxidant properties of field-grown leaves, in vitro leaves and in vitro callus cultures of the blackberry ‘Čačanska Bestrna’ and blueberry ‘Toro’. In vitro shoots of the selected genotypes were grown either on original Murashige and Skoog (MS) medium containing 1 mg/L BA, 0.1 mg/L IBA and 0.1 mg/L GA3 (‘Čačanska Bestrna’) or on MS medium with macroelements reduced to ½, 2 mg/L zeatin and 0.2 mg/L IAA (‘Toro’). Callus cultures were induced from in vitro leaves and established on MS medium with 2 mg/L BA and 2 mg/L 2,4-D (‘Čačanska Bestrna’) or MS medium with half strength macroelements, 2 mg/L BA, 2 mg/L 2,4-D and 1 mg/L NAA (‘Toro’). Total phenolic (TPC) and flavonoid content (TFC) were the highest in blueberry leaves, whereas low TPC and TFC values were obtained in callus cultures of both cultivars. A higher content of PCs in blueberry leaves compared to blackberry leaves was determined by the UHPLC-DAD MS/MS technique. Quercetin derivatives and phenolic acids were the dominant PCs in the leaves of both berries, whereas gallocatechin was present in a significant amount in blueberry leaves. Callus cultures of both berries had a specific PC profile, with none detected in the leaves except quercetin-3-O-glucoside and quercetin-3-O-rutinoside. Blackberry leaves showed the best antioxidant properties as estimated by ferric reducing power (FRP), ABTS•+ and DPPH• scavenging activity assays. Callus cultures of both berries exhibited three to five times lower ABTS•+ and ten to seventeen times lower DPPH• scavenging activity compared to corresponding leaves. The analyzed leaves and callus cultures can be a good source of PCs with good antioxidant properties and specific phenolics, respectively, for applications in the food and pharmaceutical industries.",
publisher = "Basel: MDPI",
journal = "Horticulturae",
title = "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry",
number = "11",
volume = "7",
doi = "10.3390/horticulturae7110420",
pages = "420"
}

Kolarević, T., Milinčić, D. D., Vujović, T., Gašić, U., Prokić, L., Kostić, A. Ž., Cerović, R., Stanojević, S. P., Tešić, Ž. Lj.,& Pešić, M. B.. (2021). Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae
Basel: MDPI., 7(11), 420.
https://doi.org/10.3390/horticulturae7110420

Kolarević T, Milinčić DD, Vujović T, Gašić U, Prokić L, Kostić AŽ, Cerović R, Stanojević SP, Tešić ŽL, Pešić MB. Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry. in Horticulturae. 2021;7(11):420.
doi:10.3390/horticulturae7110420 .

Kolarević, Tijana, Milinčić, Danijel D., Vujović, Tatjana, Gašić, Uroš, Prokić, Ljiljana, Kostić, Aleksandar Ž., Cerović, Radosav, Stanojević, Slađana P., Tešić, Živoslav Lj., Pešić, Mirjana B., "Phenolic Compounds and Antioxidant Properties of Field-Grown and In Vitro Leaves, and Calluses in Blackberry and Blueberry" in Horticulturae, 7, no. 11 (2021):420,
https://doi.org/10.3390/horticulturae7110420 . .

TY  - CONF
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Vujičić, Milorad
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Milošević, Snežana
PY  - 2021
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4898
AB  - Impatiens walleriana (Balsaminaceae) is a worldwide popular horticultural plant and commercially produced in Serbia for many years. Plants have high requirements for the presence of water in the substrate, which deficiency leads to a rapid drop in the cells turgor pressure and tissue dehydration. This is the main problem in commercial production of these plants, especially during transport and sale process when plants are not always sufficiently supplied with water. We assessed the drought and recovery effect on growth, physiological and biochemical parameters in I. walleriana (Xtreme Scarlet) grown in chamber under controlled physical conditions (ex vitro). Plants were 74 days old when the drought stress was imposed. We examined: control plants – grown under optimal watering (37%-40% of soil moisture content), drought stressed plants – (25%, 15%, 10% and 5% of soil moisture content), and recovery plants – rehydrated for four days to reach optimum soil moisture content (37%-40%). Drought reduces fresh (FW) and dry weight (DW) of shoots, as well as total leaf area and shoots water potential. Drought significantly increased abscisic acid (ABA) content in the leaves in order to reduce transpiration rate and preserve water in it᾽s tissue. The concentration of hydrogen peroxide (H2O2) and malondialdehyde (MDA) were increased in drought-stressed plants, as a consequence of drought induced oxidative stress in the plant cells. The activity of antioxidant enzymes (superoxide dismutase - SOD, peroxidase - POX and catalase - CAT) contributed to neutralizing negative effects of oxidative stress on plant growth, while the different SOD and POX isoforms were detected on gel. Recovery treatment neutralised negative effect of drought on growth, physiological and biochemical parameters analysed in I. walleriana to a certain extent.
PB  - Brussels: European Plant Science Organisation (EPSO)
C3  - Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy
T1  - Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro
SP  - 240
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4898
ER  - 

@conference{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Vujičić, Milorad and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Milošević, Snežana",
year = "2021",
abstract = "Impatiens walleriana (Balsaminaceae) is a worldwide popular horticultural plant and commercially produced in Serbia for many years. Plants have high requirements for the presence of water in the substrate, which deficiency leads to a rapid drop in the cells turgor pressure and tissue dehydration. This is the main problem in commercial production of these plants, especially during transport and sale process when plants are not always sufficiently supplied with water. We assessed the drought and recovery effect on growth, physiological and biochemical parameters in I. walleriana (Xtreme Scarlet) grown in chamber under controlled physical conditions (ex vitro). Plants were 74 days old when the drought stress was imposed. We examined: control plants – grown under optimal watering (37%-40% of soil moisture content), drought stressed plants – (25%, 15%, 10% and 5% of soil moisture content), and recovery plants – rehydrated for four days to reach optimum soil moisture content (37%-40%). Drought reduces fresh (FW) and dry weight (DW) of shoots, as well as total leaf area and shoots water potential. Drought significantly increased abscisic acid (ABA) content in the leaves in order to reduce transpiration rate and preserve water in it᾽s tissue. The concentration of hydrogen peroxide (H2O2) and malondialdehyde (MDA) were increased in drought-stressed plants, as a consequence of drought induced oxidative stress in the plant cells. The activity of antioxidant enzymes (superoxide dismutase - SOD, peroxidase - POX and catalase - CAT) contributed to neutralizing negative effects of oxidative stress on plant growth, while the different SOD and POX isoforms were detected on gel. Recovery treatment neutralised negative effect of drought on growth, physiological and biochemical parameters analysed in I. walleriana to a certain extent.",
publisher = "Brussels: European Plant Science Organisation (EPSO)",
journal = "Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy",
title = "Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro",
pages = "240",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4898"
}

Đurić, M., Subotić, A., Prokić, L., Vujičić, M., Trifunović-Momčilov, M., Cingel, A.,& Milošević, S.. (2021). Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro. in Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy
Brussels: European Plant Science Organisation (EPSO)., 240.
https://hdl.handle.net/21.15107/rcub_ibiss_4898

Đurić M, Subotić A, Prokić L, Vujičić M, Trifunović-Momčilov M, Cingel A, Milošević S. Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro. in Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy. 2021;:240.
https://hdl.handle.net/21.15107/rcub_ibiss_4898 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Vujičić, Milorad, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Milošević, Snežana, "Drought stress and recovery effects on morpho-physiological and biochemical responses in Impatiens walleriana grown ex vitro" in Abstract Book of the Plant Biology Europe 2021; 2021 Jun 28 - Jul 1; Turin, Italy (2021):240,
https://hdl.handle.net/21.15107/rcub_ibiss_4898 .

TY  - JOUR
AU  - Đurić, Marija
AU  - Subotić, Angelina
AU  - Prokić, Ljiljana
AU  - Trifunović-Momčilov, Milana
AU  - Cingel, Aleksandar
AU  - Vujičić, Milorad
AU  - Milošević, Snežana
PY  - 2020
UR  - https://www.mdpi.com/2223-7747/9/11/1559
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4008
AB  - This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants—grown under optimal watering (35%–37% of soil moisture content), drought-stressed plants—non-irrigated to reach 15% and 5% of soil moisture content and recovery plants—rehydrated for four days to reach optimal soil moisture content. Drought reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Drought up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (NCED4) and abscisic aldehyde oxidase 2 (AAO2) and catabolic gene ABA 8′-hydroxylase 3 (ABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the drought was not accompanied with increased amino acid proline content. We detected an increase in chlorophyll, carotenoid, total polyphenols and flavonols content under drought conditions, as well as malondialdehyde, hydrogen peroxide and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity. Increased antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) throughout drought were also determined. Recovery treatment was significant for neutralizing drought effect on growth parameters, shoot water potential, proline content and genes expression.
PB  - MDPI AG
T2  - Plants
T1  - Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro
IS  - 11
VL  - 9
DO  - 10.3390/plants9111559
SP  - 1559
ER  - 

@article{
author = "Đurić, Marija and Subotić, Angelina and Prokić, Ljiljana and Trifunović-Momčilov, Milana and Cingel, Aleksandar and Vujičić, Milorad and Milošević, Snežana",
year = "2020",
abstract = "This study was carried out to examine the drought effect on development, physiological, biochemical and molecular parameters in Impatiens walleriana grown ex vitro. Experiment design included three treatments: Control plants—grown under optimal watering (35%–37% of soil moisture content), drought-stressed plants—non-irrigated to reach 15% and 5% of soil moisture content and recovery plants—rehydrated for four days to reach optimal soil moisture content. Drought reduced fresh weight, total leaf area, as well as dry weight of I. walleriana shoots. Drought up-regulated expression of abscisic acid (ABA) biosynthesis genes 9-cis-epoxycarotenoid dioxygenase 4 (NCED4) and abscisic aldehyde oxidase 2 (AAO2) and catabolic gene ABA 8′-hydroxylase 3 (ABA8ox3) which was followed by increased ABA content in the leaves. Decrement in water potential of shoots during the drought was not accompanied with increased amino acid proline content. We detected an increase in chlorophyll, carotenoid, total polyphenols and flavonols content under drought conditions, as well as malondialdehyde, hydrogen peroxide and DPPH (1,1′-diphenyl-2-picrylhydrazyl) activity. Increased antioxidant enzyme activities (superoxide dismutase, peroxidase and catalase) throughout drought were also determined. Recovery treatment was significant for neutralizing drought effect on growth parameters, shoot water potential, proline content and genes expression.",
publisher = "MDPI AG",
journal = "Plants",
title = "Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro",
number = "11",
volume = "9",
doi = "10.3390/plants9111559",
pages = "1559"
}

Đurić, M., Subotić, A., Prokić, L., Trifunović-Momčilov, M., Cingel, A., Vujičić, M.,& Milošević, S.. (2020). Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro. in Plants
MDPI AG., 9(11), 1559.
https://doi.org/10.3390/plants9111559

Đurić M, Subotić A, Prokić L, Trifunović-Momčilov M, Cingel A, Vujičić M, Milošević S. Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro. in Plants. 2020;9(11):1559.
doi:10.3390/plants9111559 .

Đurić, Marija, Subotić, Angelina, Prokić, Ljiljana, Trifunović-Momčilov, Milana, Cingel, Aleksandar, Vujičić, Milorad, Milošević, Snežana, "Morpho-Physiological and Molecular Evaluation of Drought and Recovery in Impatiens walleriana Grown Ex Vitro" in Plants, 9, no. 11 (2020):1559,
https://doi.org/10.3390/plants9111559 . .

TY  - JOUR
AU  - Vidović, Marija
AU  - Morina, Filis
AU  - Milić-Komić, Sonja
AU  - Vuleta, Ana
AU  - Zechmann, Bernd
AU  - Prokić, Ljiljana
AU  - Veljović Jovanović, Sonja
AU  - Mock, H. P.
PY  - 2016
UR  - http://doi.wiley.com/10.1111/plb.12429
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2988
AB  - Hydrogen peroxide is an important signalling molecule, involved in regulation of numerous metabolic processes in plants. The most important sources of H2O2 in photosynthetically active cells are chloroplasts and peroxisomes. Here we employed variegated Pelargonium zonale to characterise and compare enzymatic and non-enzymatic components of the antioxidative system in autotrophic and heterotrophic leaf tissues at (sub)cellular level under optimal growth conditions. The results revealed that both leaf tissues had specific strategies to regulate H2O2 levels. In photosynthetic cells, the redox regulatory system was based on ascorbate, and on the activities of thylakoid-bound ascorbate peroxidase (tAPX) and catalase. In this leaf tissue, ascorbate was predominantly localised in the nucleus, peroxisomes, plastids and mitochondria. On the other hand, non-photosynthetic cells contained higher glutathione content, mostly located in mitochondria. The enzymatic antioxidative system in non-photosynthetic cells relied on the ascorbate–glutathione cycle and both Mn and Cu/Zn superoxide dismutase. Interestingly, higher content of ascorbate and glutathione, and higher activities of APX in the cytosol of non-photosynthetic leaf cells compared to the photosynthetic ones, suggest the importance of this compartment in H2O2 regulation. Together, these results imply different regulation of processes linked with H2O2 signalling at subcellular level. Thus, we propose green-white variegated leaves as an excellent system for examination of redox signal transduction and redox communication between two cell types, autotrophic and heterotrophic, within the same organ.
PB  - Blackwell Publishing Ltd
T2  - Plant Biology
T1  - Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants
IS  - 4
VL  - 18
DO  - 10.1111/plb.12429
SP  - 669
EP  - 680
ER  - 

@article{
author = "Vidović, Marija and Morina, Filis and Milić-Komić, Sonja and Vuleta, Ana and Zechmann, Bernd and Prokić, Ljiljana and Veljović Jovanović, Sonja and Mock, H. P.",
year = "2016",
abstract = "Hydrogen peroxide is an important signalling molecule, involved in regulation of numerous metabolic processes in plants. The most important sources of H2O2 in photosynthetically active cells are chloroplasts and peroxisomes. Here we employed variegated Pelargonium zonale to characterise and compare enzymatic and non-enzymatic components of the antioxidative system in autotrophic and heterotrophic leaf tissues at (sub)cellular level under optimal growth conditions. The results revealed that both leaf tissues had specific strategies to regulate H2O2 levels. In photosynthetic cells, the redox regulatory system was based on ascorbate, and on the activities of thylakoid-bound ascorbate peroxidase (tAPX) and catalase. In this leaf tissue, ascorbate was predominantly localised in the nucleus, peroxisomes, plastids and mitochondria. On the other hand, non-photosynthetic cells contained higher glutathione content, mostly located in mitochondria. The enzymatic antioxidative system in non-photosynthetic cells relied on the ascorbate–glutathione cycle and both Mn and Cu/Zn superoxide dismutase. Interestingly, higher content of ascorbate and glutathione, and higher activities of APX in the cytosol of non-photosynthetic leaf cells compared to the photosynthetic ones, suggest the importance of this compartment in H2O2 regulation. Together, these results imply different regulation of processes linked with H2O2 signalling at subcellular level. Thus, we propose green-white variegated leaves as an excellent system for examination of redox signal transduction and redox communication between two cell types, autotrophic and heterotrophic, within the same organ.",
publisher = "Blackwell Publishing Ltd",
journal = "Plant Biology",
title = "Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants",
number = "4",
volume = "18",
doi = "10.1111/plb.12429",
pages = "669-680"
}

Vidović, M., Morina, F., Milić-Komić, S., Vuleta, A., Zechmann, B., Prokić, L., Veljović Jovanović, S.,& Mock, H. P.. (2016). Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants. in Plant Biology
Blackwell Publishing Ltd., 18(4), 669-680.
https://doi.org/10.1111/plb.12429

Vidović M, Morina F, Milić-Komić S, Vuleta A, Zechmann B, Prokić L, Veljović Jovanović S, Mock HP. Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants. in Plant Biology. 2016;18(4):669-680.
doi:10.1111/plb.12429 .

Vidović, Marija, Morina, Filis, Milić-Komić, Sonja, Vuleta, Ana, Zechmann, Bernd, Prokić, Ljiljana, Veljović Jovanović, Sonja, Mock, H. P., "Characterisation of antioxidants in photosynthetic and non-photosynthetic leaf tissues of variegated Pelargonium zonale plants" in Plant Biology, 18, no. 4 (2016):669-680,
https://doi.org/10.1111/plb.12429 . .