TY  - CONF
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Raspor, Martin
AU  - Sajid, Sumbal
AU  - Savić, Jelena
AU  - Devrnja, Nina
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5364
AB  - This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.
PB  - Belgrade: Serbian Plant Physiology Society
C3  - 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
T1  - Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5364
ER  - 

@conference{
author = "Ćosić, Tatjana and Motyka, Václav and Raspor, Martin and Sajid, Sumbal and Savić, Jelena and Devrnja, Nina and Dobrev, Petre I. and Ninković, Slavica",
year = "2022",
abstract = "This study fathoms a comprehensive hormone profiling of four growth stages of kohlrabi
(Brassica oleracea var. gongylodes) seedlings in vitro (T1-T4), including the effects of cytokinins, CKs
(trans-zeatin, transZ; thidiazuron, TDZ), and high sucrose concentration (6% and 9%). Depending
on the type of investigated phytohormone and its metabolite, individual changes were observed
on a time scale, showing specific hormone profiles. A significant increase in the content of endogenous
zeatin nucleobases and gibberellin GA20 was detected at T2 stage (plantlets with two
emerged leaves). However, levels of jasmonic acid, JA-isoleucine, indole-3-acetic acid (IAA) and
indole-3-acetamide significantly increased as development progressed (T3), whereas content of
most of analysed IAA metabolites decreased. Supplementing growth media with CKs induced de
novo shoot formation during seedling development, and both CK and sucrose treatments caused
significant changes in levels of most of the phytohormone groups at each developmental stage
compared to control. Principal component analysis indicated that sucrose treatments, particularly
9%, had a stronger effect on the content of endogenous hormones than the CK treatments. The
difference between two CK treatments proved to be remarkable at T1, T2, and T3 stages, while
at the last stage difference was much smaller. The joint application of CKs and high sucrose concentration
showed diverse relations depending on the metabolite, growth stage and amount of
sucrose used. The most striking increase was induced in T4 for zeatin nucleobases when transZ
and 9% sucrose were applied. Our results indicate specific patterns of metabolic balance during
kohlrabi in vitro development and regeneration, revealing a complex interplay of different phytohormonal
groups with CKs and sucrose.",
publisher = "Belgrade: Serbian Plant Physiology Society",
journal = "4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia",
title = "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose",
pages = "24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5364"
}

Ćosić, T., Motyka, V., Raspor, M., Sajid, S., Savić, J., Devrnja, N., Dobrev, P. I.,& Ninković, S.. (2022). Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia
Belgrade: Serbian Plant Physiology Society., 24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364

Ćosić T, Motyka V, Raspor M, Sajid S, Savić J, Devrnja N, Dobrev PI, Ninković S. Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose. in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia. 2022;:24.
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .

Ćosić, Tatjana, Motyka, Václav, Raspor, Martin, Sajid, Sumbal, Savić, Jelena, Devrnja, Nina, Dobrev, Petre I., Ninković, Slavica, "Phytohormone homeostasis in in vitro grown kohlrabi: the effect of cytokinins and sucrose" in 4th International Conference on Plant Biology [and] 23rd SPPS Meeting; 2022 Oct 6-8; Belgrade, Serbia (2022):24,
https://hdl.handle.net/21.15107/rcub_ibiss_5364 .

TY  - JOUR
AU  - Ćosić, Tatjana
AU  - Motyka, Václav
AU  - Savić, Jelena
AU  - Raspor, Martin
AU  - Marković, Marija
AU  - Dobrev, Petre I.
AU  - Ninković, Slavica
PY  - 2021
UR  - https://www.nature.com/articles/s41598-021-85932-w
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4194
AB  - Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.
PB  - Springer Science and Business Media LLC
T2  - Scientific Reports
T1  - Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi
IS  - 1
VL  - 11
DO  - 10.1038/s41598-021-85932-w
SP  - 6494
ER  - 

@article{
author = "Ćosić, Tatjana and Motyka, Václav and Savić, Jelena and Raspor, Martin and Marković, Marija and Dobrev, Petre I. and Ninković, Slavica",
year = "2021",
abstract = "Cross-talk between phytohormones and sugars is intensely involved in plant metabolism, growth and regeneration. We documented alterations in cytokinin (CK) homeostasis in four developmental stages during de novo shoot organogenesis (DNSO) of kohlrabi ( Brassica oleracea var. gongylodes cv. Vienna Purple) seedlings induced by exogenous CKs, trans -zeatin ( trans Z) and thidiazuron (TDZ), added together with elevated sucrose concentration (6% and 9%). Significant impact of CK and sucrose treatment and their interaction was recorded in all investigated stages, including plantlet development before calli formation (T1 and T2), calli formation (T3) and shoot regeneration (T4). Results showed remarkable increase in total CK levels for trans Z treatment, particularly with 9% sucrose. This trend was observed for all physiological and structural groups of CKs. Application of TDZ contributed to little or no increase in CK levels regardless of sucrose concentration. Analysis of expression profiles of organogenesis-related genes involved in auxin transport, CK response, shoot apical meristem formation and cell division revealed that higher sugar concentration significantly downregulated the analysed genes, particularly in T3. This continued on TDZ, but trans Z induced an opposite effect with 9% sucrose in T4, increasing gene activity. Our results demonstrated that phytohormone metabolism might be triggered by sucrose signalling in kohlrabi DNSO.",
publisher = "Springer Science and Business Media LLC",
journal = "Scientific Reports",
title = "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi",
number = "1",
volume = "11",
doi = "10.1038/s41598-021-85932-w",
pages = "6494"
}

Ćosić, T., Motyka, V., Savić, J., Raspor, M., Marković, M., Dobrev, P. I.,& Ninković, S.. (2021). Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports
Springer Science and Business Media LLC., 11(1), 6494.
https://doi.org/10.1038/s41598-021-85932-w

Ćosić T, Motyka V, Savić J, Raspor M, Marković M, Dobrev PI, Ninković S. Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi. in Scientific Reports. 2021;11(1):6494.
doi:10.1038/s41598-021-85932-w .

Ćosić, Tatjana, Motyka, Václav, Savić, Jelena, Raspor, Martin, Marković, Marija, Dobrev, Petre I., Ninković, Slavica, "Sucrose interferes with endogenous cytokinin homeostasis and expression of organogenesis-related genes during de novo shoot organogenesis in kohlrabi" in Scientific Reports, 11, no. 1 (2021):6494,
https://doi.org/10.1038/s41598-021-85932-w . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Kaleri, Abdul Rasheed
AU  - Ninković, Slavica
AU  - Tubić, Ljiljana
AU  - Cingel, Aleksandar
AU  - Ćosić, Tatjana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4303
AB  - De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs
IS  - 16
VL  - 22
DO  - 10.3390/ijms22168554
SP  - 8554
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Kaleri, Abdul Rasheed and Ninković, Slavica and Tubić, Ljiljana and Cingel, Aleksandar and Ćosić, Tatjana",
year = "2021",
abstract = "De novo shoot organogenesis (DNSO) is a procedure commonly used for the in vitro
regeneration of shoots from a variety of plant tissues. Shoot regeneration occurs on nutrient media
supplemented with the plant hormones cytokinin (CK) and auxin, which play essential roles in this
process, and genes involved in their signaling cascades act as master regulators of the different phases
of shoot regeneration. In the last 20 years, the genetic regulation of DNSO has been characterized in
detail. However, as of today, the CK and auxin signaling events associated with shoot regeneration
are often interpreted as a consequence of these hormones simply being present in the regeneration
media, whereas the roles for their prior uptake and transport into the cultivated plant tissues are
generally overlooked. Additionally, sucrose, commonly added to the regeneration media as a carbon
source, plays a signaling role and has been recently shown to interact with CK and auxin and to
affect the efficiency of shoot regeneration. In this review, we provide an integrative interpretation
of the roles for CK and auxin in the process of DNSO, adding emphasis on their uptake from the
regeneration media and their interaction with sucrose present in the media to their complex signaling
outputs that mediate shoot regeneration.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs",
number = "16",
volume = "22",
doi = "10.3390/ijms22168554",
pages = "8554"
}

Raspor, M., Motyka, V., Kaleri, A. R., Ninković, S., Tubić, L., Cingel, A.,& Ćosić, T.. (2021). Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences
Basel: MDPI., 22(16), 8554.
https://doi.org/10.3390/ijms22168554

Raspor M, Motyka V, Kaleri AR, Ninković S, Tubić L, Cingel A, Ćosić T. Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs. in International Journal of Molecular Sciences. 2021;22(16):8554.
doi:10.3390/ijms22168554 .

Raspor, Martin, Motyka, Václav, Kaleri, Abdul Rasheed, Ninković, Slavica, Tubić, Ljiljana, Cingel, Aleksandar, Ćosić, Tatjana, "Integrating the Roles for Cytokinin and Auxin in De Novo Shoot Organogenesis: From Hormone Uptake to Signaling Outputs" in International Journal of Molecular Sciences, 22, no. 16 (2021):8554,
https://doi.org/10.3390/ijms22168554 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Václav
AU  - Dobrev, Petre I.
AU  - Marković, Marija
AU  - Milošević, Snežana
AU  - Jevremović, Slađana
AU  - Dragićević, Ivana Č.
AU  - Subotić, Angelina
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4649
AB  - Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses
considerably affect production and distribution of phytohormones as the stress signal triggers. The
homeostasis of plant hormones is controlled by their de novo synthesis and catabolism. The aim of this
work was to analyse the contents of total and individual groups of endogenous cytokinins (CKs) as well
as indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown in vitro on graded NaCl
concentrations (0, 50, 100, 150, 200 mM). The levels of endogenous stress hormones including abscisic
acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were also detected. The elevated contents of total
CKs were found in all analysed centaury shoots. Furthermore, increased amounts of all five CK groups,
as well as enhanced total CKs were revealed on graded NaCl concentrations in non-transformed and
AtCKX roots. All analysed AtCKX centaury lines exhibited decreased amounts of endogenous IAA
in shoots and roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation
in the shoots and roots of all AtCKX lines. In shoots and roots of both non-transformed and AtCKX
transgenic centaury plants, salinity was associated with an increase of ABA and JA and a decrease of
SA content.
PB  - Springer Nature
T2  - Scientific Reports
T1  - Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro
VL  - 11
DO  - 10.1038/s41598-021-00866-7
SP  - 21471
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Motyka, Václav and Dobrev, Petre I. and Marković, Marija and Milošević, Snežana and Jevremović, Slađana and Dragićević, Ivana Č. and Subotić, Angelina",
year = "2021",
abstract = "Plant hormones regulate numerous developmental and physiological processes. Abiotic stresses
considerably affect production and distribution of phytohormones as the stress signal triggers. The
homeostasis of plant hormones is controlled by their de novo synthesis and catabolism. The aim of this
work was to analyse the contents of total and individual groups of endogenous cytokinins (CKs) as well
as indole-3-acetic acid (IAA) in AtCKX overexpressing centaury plants grown in vitro on graded NaCl
concentrations (0, 50, 100, 150, 200 mM). The levels of endogenous stress hormones including abscisic
acid (ABA), salicylic acid (SA) and jasmonic acid (JA) were also detected. The elevated contents of total
CKs were found in all analysed centaury shoots. Furthermore, increased amounts of all five CK groups,
as well as enhanced total CKs were revealed on graded NaCl concentrations in non-transformed and
AtCKX roots. All analysed AtCKX centaury lines exhibited decreased amounts of endogenous IAA
in shoots and roots. Consequently, the IAA/bioactive CK forms ratios showed a significant variation
in the shoots and roots of all AtCKX lines. In shoots and roots of both non-transformed and AtCKX
transgenic centaury plants, salinity was associated with an increase of ABA and JA and a decrease of
SA content.",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro",
volume = "11",
doi = "10.1038/s41598-021-00866-7",
pages = "21471"
}

Trifunović-Momčilov, M., Motyka, V., Dobrev, P. I., Marković, M., Milošević, S., Jevremović, S., Dragićević, I. Č.,& Subotić, A.. (2021). Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro. in Scientific Reports
Springer Nature., 11, 21471.
https://doi.org/10.1038/s41598-021-00866-7

Trifunović-Momčilov M, Motyka V, Dobrev PI, Marković M, Milošević S, Jevremović S, Dragićević IČ, Subotić A. Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro. in Scientific Reports. 2021;11:21471.
doi:10.1038/s41598-021-00866-7 .

Trifunović-Momčilov, Milana, Motyka, Václav, Dobrev, Petre I., Marković, Marija, Milošević, Snežana, Jevremović, Slađana, Dragićević, Ivana Č., Subotić, Angelina, "Phytohormone profiles in non‑transformed and AtCKX transgenic centaury (Centaurium erythraea Rafn) shoots and roots in response to salinity stress in vitro" in Scientific Reports, 11 (2021):21471,
https://doi.org/10.1038/s41598-021-00866-7 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Malbeck, Jiří
AU  - Dobrev, Petre I.
AU  - Zdravković-Korać, Snežana
AU  - Simonović, Ana
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Zahajská, Lenka
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3605
AB  - Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.
T2  - Journal of Plant Growth Regulation
T1  - Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization
VL  - 40
DO  - 10.1007/s00344-020-10080-w
SP  - 37
EP  - 47
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Malbeck, Jiří and Dobrev, Petre I. and Zdravković-Korać, Snežana and Simonović, Ana and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Zahajská, Lenka and Tadić, Vojin and Dragićević, Ivana",
year = "2021",
abstract = "Over the last years, cytokinin deficiency has been studied in a variety of plant species, using transgenic expression of cytokinin oxidase/dehydrogenase genes (CKX). In tobacco and Arabidopsis thaliana, overexpression of A. thaliana CKX genes caused changes in plant morphology known as the "cytokinin deficiency syndrome", including stunted shoot growth, decreased apical dominance, and enhanced root growth. For a valid comparison with the previously obtained in vitro grown AtCKX2-transgenic potato lines, we report here on the construction of transgenic lines of the cultivar Désirée constitutively expressing the gene AtCKX1 under control of the CaMV 35S promoter. Three lines expressing the transgene in both shoot and root tissues were used for analysis. Analyses of CKX activity of tissue extracts, cytokinin levels, morphology, and tuberization parameters revealed similarities as well as differences from the AtCKX2-transgenic plants, including lower levels of CKX activity, and more pronounced morphological effects of cytokinin deficiency. One of the three AtCKX1-overexpressing potato lines was able to start forming tubers before 30 days of growth in long day conditions (16 h/8 h photoperiod) as has been previously observed in some AtCKX2 overexpressors. The extension of the experimental period to 120 days of growth in vitro showed that the other two AtCKX1-overexpressing lines showed impaired tuberization in terms of the number of tubers per plant, and tuber size. We conclude that cytokinin deficiency has distinct effects on tuber induction and tuber initiation/growth, possibly mediated by distinct signaling mechanisms.",
journal = "Journal of Plant Growth Regulation",
title = "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization",
volume = "40",
doi = "10.1007/s00344-020-10080-w",
pages = "37-47"
}

Raspor, M., Motyka, V., Ninković, S., Malbeck, J., Dobrev, P. I., Zdravković-Korać, S., Simonović, A., Ćosić, T., Cingel, A., Savić, J., Zahajská, L., Tadić, V.,& Dragićević, I.. (2021). Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation, 40, 37-47.
https://doi.org/10.1007/s00344-020-10080-w

Raspor M, Motyka V, Ninković S, Malbeck J, Dobrev PI, Zdravković-Korać S, Simonović A, Ćosić T, Cingel A, Savić J, Zahajská L, Tadić V, Dragićević I. Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization. in Journal of Plant Growth Regulation. 2021;40:37-47.
doi:10.1007/s00344-020-10080-w .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Malbeck, Jiří, Dobrev, Petre I., Zdravković-Korać, Snežana, Simonović, Ana, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Zahajská, Lenka, Tadić, Vojin, Dragićević, Ivana, "Overexpressing AtCKX1 in Potato Plants grown In Vitro: The Effects on Cytokinin Composition and Tuberization" in Journal of Plant Growth Regulation, 40 (2021):37-47,
https://doi.org/10.1007/s00344-020-10080-w . .

TY  - CHAP
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Václav
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4064
AB  - The production and breeding of plants with desired properties are possible by a
fundamental biotechnological technique, genetic engineering. Applying and developing
of genetic engineering procedures also enable preservation and improvement
of plant species endangered in nature, including medicinal plant common centaury
(Centaurium erythraea Rafn.). Numerous developmental processes in plants are
controlled by cytokinins (CKs). The only so far known enzyme involved in CK
catabolism is cytokinin oxidase/dehydrogenase (CKX). Genes coding for two
Arabidopsis CKX isoforms, AtCKX1 and AtCKX2, were successfully introduced into
centaury root explants. Subsequently, the contents of endogenous CKs in AtCKXoverexpressing
centaury plants grown in vitro were investigated. Simultaneous
secondary metabolite analyses showed antibacterial and antifungal activity of
transgenic centaury plants and suggested their use as potential producers of anticancer
compounds. Considering that centaury can inhabit saline soils in natural
habitats, following investigations included evaluation of salinity tolerance in vitro.
All obtained and summarized results indicated that transgenic AtCKX centaury
plants could serve as a suitable model for studies of numerous physiological and
developmental processes under endogenous phytohormonal control.
PB  - IntechOpen
T2  - Plant Breeding: Current and Future Views
T1  - Transgenic AtCKX Centaury Plants Grown In Vitro
DO  - 10.5772/intechopen.94836
ER  - 

@inbook{
editor = "Abdurakhmonov, Ibrokhim Y.",
author = "Trifunović-Momčilov, Milana and Motyka, Václav",
year = "2021",
abstract = "The production and breeding of plants with desired properties are possible by a
fundamental biotechnological technique, genetic engineering. Applying and developing
of genetic engineering procedures also enable preservation and improvement
of plant species endangered in nature, including medicinal plant common centaury
(Centaurium erythraea Rafn.). Numerous developmental processes in plants are
controlled by cytokinins (CKs). The only so far known enzyme involved in CK
catabolism is cytokinin oxidase/dehydrogenase (CKX). Genes coding for two
Arabidopsis CKX isoforms, AtCKX1 and AtCKX2, were successfully introduced into
centaury root explants. Subsequently, the contents of endogenous CKs in AtCKXoverexpressing
centaury plants grown in vitro were investigated. Simultaneous
secondary metabolite analyses showed antibacterial and antifungal activity of
transgenic centaury plants and suggested their use as potential producers of anticancer
compounds. Considering that centaury can inhabit saline soils in natural
habitats, following investigations included evaluation of salinity tolerance in vitro.
All obtained and summarized results indicated that transgenic AtCKX centaury
plants could serve as a suitable model for studies of numerous physiological and
developmental processes under endogenous phytohormonal control.",
publisher = "IntechOpen",
journal = "Plant Breeding: Current and Future Views",
booktitle = "Transgenic AtCKX Centaury Plants Grown In Vitro",
doi = "10.5772/intechopen.94836"
}

Abdurakhmonov, I. Y., Trifunović-Momčilov, M.,& Motyka, V.. (2021). Transgenic AtCKX Centaury Plants Grown In Vitro. in Plant Breeding: Current and Future Views
IntechOpen..
https://doi.org/10.5772/intechopen.94836

Abdurakhmonov IY, Trifunović-Momčilov M, Motyka V. Transgenic AtCKX Centaury Plants Grown In Vitro. in Plant Breeding: Current and Future Views. 2021;.
doi:10.5772/intechopen.94836 .

Abdurakhmonov, Ibrokhim Y., Trifunović-Momčilov, Milana, Motyka, Václav, "Transgenic AtCKX Centaury Plants Grown In Vitro" in Plant Breeding: Current and Future Views (2021),
https://doi.org/10.5772/intechopen.94836 . .

TY  - JOUR
AU  - Raspor, Martin
AU  - Motyka, Václav
AU  - Ninković, Slavica
AU  - Dobrev, Petre I.
AU  - Malbeck, Jiří
AU  - Ćosić, Tatjana
AU  - Cingel, Aleksandar
AU  - Savić, Jelena
AU  - Tadić, Vojin
AU  - Dragićević, Ivana
PY  - 2020
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC7044434
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3623
AB  - A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.
T2  - Scientific Reports
T1  - Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.
IS  - 1
VL  - 10
DO  - 10.1038/s41598-020-60412-9
SP  - 3437
ER  - 

@article{
author = "Raspor, Martin and Motyka, Václav and Ninković, Slavica and Dobrev, Petre I. and Malbeck, Jiří and Ćosić, Tatjana and Cingel, Aleksandar and Savić, Jelena and Tadić, Vojin and Dragićević, Ivana",
year = "2020",
abstract = "A number of scientific reports published to date contain data on endogenous levels of various phytohormones in potato (Solanum tuberosum L.) but a complete cytokinin profile of potato tissues, that would include data on all particular molecular forms of cytokinin, has still been missing. In this work, endogenous levels of all analytically detectable isoprenoid cytokinins, as well as the auxin indole-3-acetic acid (IAA), and abscisic acid (ABA) have been determined in shoots and roots of 30 day old in vitro grown potato (cv. Désirée). The results presented here are generally similar to other data reported for in vitro grown potato plants, whereas greenhouse-grown plants typically contain lower levels of ABA, possibly indicating that in vitro grown potato is exposed to chronic stress. Cytokinin N-glucosides, particularly N7-glucosides, are the dominant cytokinin forms in both shoots and roots of potato, whereas nucleobases, as the bioactive forms of cytokinins, comprise a low proportion of cytokinin levels in tissues of potato. Differences in phytohormone composition between shoots and roots of potato suggest specific patterns of transport and/or differences in tissue-specific metabolism of plant hormones. These results represent a contribution to understanding the hormonomics of potato, a crop species of extraordinary economic importance.",
journal = "Scientific Reports",
title = "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.",
number = "1",
volume = "10",
doi = "10.1038/s41598-020-60412-9",
pages = "3437"
}

Raspor, M., Motyka, V., Ninković, S., Dobrev, P. I., Malbeck, J., Ćosić, T., Cingel, A., Savić, J., Tadić, V.,& Dragićević, I.. (2020). Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports, 10(1), 3437.
https://doi.org/10.1038/s41598-020-60412-9

Raspor M, Motyka V, Ninković S, Dobrev PI, Malbeck J, Ćosić T, Cingel A, Savić J, Tadić V, Dragićević I. Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics.. in Scientific Reports. 2020;10(1):3437.
doi:10.1038/s41598-020-60412-9 .

Raspor, Martin, Motyka, Václav, Ninković, Slavica, Dobrev, Petre I., Malbeck, Jiří, Ćosić, Tatjana, Cingel, Aleksandar, Savić, Jelena, Tadić, Vojin, Dragićević, Ivana, "Endogenous levels of cytokinins, indole-3-acetic acid and abscisic acid in in vitro grown potato: A contribution to potato hormonomics." in Scientific Reports, 10, no. 1 (2020):3437,
https://doi.org/10.1038/s41598-020-60412-9 . .

TY  - JOUR
AU  - Uzelac, Branka
AU  - Janošević, Dušica
AU  - Simonović, Ana
AU  - Motyka, Václav
AU  - Dobrev, Petre I
AU  - Budimir, Snežana
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2989
UR  - https://link.springer.com/article/10.1007%2Fs00709-015-0802-9#aboutcontent
AB  - Leaf senescence is a highly regulated final phase of leaf development preceding massive cell death. It results in the coordinated degradation of macromolecules and the subsequent nutrient relocation to other plant parts. Very little is still known about early stages of leaf senescence during normal leaf ontogeny that is not triggered by stress factors. This paper comprises an integrated study of natural leaf senescence in tobacco plants grown in vitro, using molecular, structural, and physiological information. We determined the time sequence of ultrastructural changes in mesophyll cells during leaf senescence, showing that the degradation of chloroplast ultrastructure fully correlated with changes in chlorophyll content. The earliest degenerative changes in chloroplast ultrastructure coinciding with early chromatin condensation were observed already in mature green leaves. A continuum of degradative changes in chloroplast ultrastructure, chromatin condensation and aggregation, along with progressive decrease in cytoplasm organization and electron density were observed in the course of mesophyll cells ageing. Although the total amounts of endogenous cytokinins gradually increased during leaf ontogenesis, the proportion of bioactive cytokinin forms, as well as their phosphate precursors, in total cytokinin content rapidly declined with ageing. Endogenous indole-3-acetic acid (IAA) levels were strongly reduced in senescent leaves, and a decreasing tendency was also observed for abscisic acid (ABA) levels. Senescence-associated tobacco cysteine proteases (CP, E.C. 3.4.22) CP1 and CP23 genes were induced in the initial phase of senescence. Genes encoding glutamate dehydrogenase (GDH, E.C. 1.4.1.2) and one isoform of cytosolic glutamine synthetase (GS1, E.C. 6.3.1.2) were induced in the late stage of senescence, while chloroplastic GS (GS2) gene showed a continuous decrease with leaf ageing.
PB  - Springer-Verlag Wien
T2  - Protoplasma
T1  - Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.
IS  - 2
VL  - 253
DO  - 10.1007/s00709-015-0802-9
SP  - 259
EP  - 75
ER  - 

@article{
author = "Uzelac, Branka and Janošević, Dušica and Simonović, Ana and Motyka, Václav and Dobrev, Petre I and Budimir, Snežana",
year = "2016",
abstract = "Leaf senescence is a highly regulated final phase of leaf development preceding massive cell death. It results in the coordinated degradation of macromolecules and the subsequent nutrient relocation to other plant parts. Very little is still known about early stages of leaf senescence during normal leaf ontogeny that is not triggered by stress factors. This paper comprises an integrated study of natural leaf senescence in tobacco plants grown in vitro, using molecular, structural, and physiological information. We determined the time sequence of ultrastructural changes in mesophyll cells during leaf senescence, showing that the degradation of chloroplast ultrastructure fully correlated with changes in chlorophyll content. The earliest degenerative changes in chloroplast ultrastructure coinciding with early chromatin condensation were observed already in mature green leaves. A continuum of degradative changes in chloroplast ultrastructure, chromatin condensation and aggregation, along with progressive decrease in cytoplasm organization and electron density were observed in the course of mesophyll cells ageing. Although the total amounts of endogenous cytokinins gradually increased during leaf ontogenesis, the proportion of bioactive cytokinin forms, as well as their phosphate precursors, in total cytokinin content rapidly declined with ageing. Endogenous indole-3-acetic acid (IAA) levels were strongly reduced in senescent leaves, and a decreasing tendency was also observed for abscisic acid (ABA) levels. Senescence-associated tobacco cysteine proteases (CP, E.C. 3.4.22) CP1 and CP23 genes were induced in the initial phase of senescence. Genes encoding glutamate dehydrogenase (GDH, E.C. 1.4.1.2) and one isoform of cytosolic glutamine synthetase (GS1, E.C. 6.3.1.2) were induced in the late stage of senescence, while chloroplastic GS (GS2) gene showed a continuous decrease with leaf ageing.",
publisher = "Springer-Verlag Wien",
journal = "Protoplasma",
title = "Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.",
number = "2",
volume = "253",
doi = "10.1007/s00709-015-0802-9",
pages = "259-75"
}

Uzelac, B., Janošević, D., Simonović, A., Motyka, V., Dobrev, P. I.,& Budimir, S.. (2016). Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.. in Protoplasma
Springer-Verlag Wien., 253(2), 259-75.
https://doi.org/10.1007/s00709-015-0802-9

Uzelac B, Janošević D, Simonović A, Motyka V, Dobrev PI, Budimir S. Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro.. in Protoplasma. 2016;253(2):259-75.
doi:10.1007/s00709-015-0802-9 .

Uzelac, Branka, Janošević, Dušica, Simonović, Ana, Motyka, Václav, Dobrev, Petre I, Budimir, Snežana, "Characterization of natural leaf senescence in tobacco (Nicotiana tabacum) plants grown in vitro." in Protoplasma, 253, no. 2 (2016):259-75,
https://doi.org/10.1007/s00709-015-0802-9 . .

TY  - JOUR
AU  - Trifunović-Momčilov, Milana
AU  - Motyka, Václav
AU  - Dragićević, Ivana C.
AU  - Petrić, Marija
AU  - Jevremović, Slađana
AU  - Malbeck, Jiří
AU  - Holik, Josef
AU  - Dobrev, Petre I.
AU  - Subotić, Angelina
PY  - 2016
UR  - https://link.springer.com/article/10.1007%2Fs00344-015-9558-x
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3381
AB  - Phytohormones are important regulators of
numerous developmental and physiological processes in
plants. Spontaneous morphogenesis of the common centaury
(Centaurium erythraea Rafn.) is possible on nutrition
medium without addition of any plant growth
regulator depending solely on endogenous phytohormone
levels. Thus, this plant species represents a very good
model system for the investigation of numerous physiological
processes under phytohormonal control in vitro.
We analysed the total amount of endogenous cytokinins
(CKs) including the contents of their individual groups in
shoots and roots of C. erythraea plants grown in vitro. The
total amount of endogenous CKs was 1.4 times higher in
shoots than in roots. Inactive or weakly active N-glucosides
found to predominate in both organs of centaury
plants, whereas free bases and O-glucosides represented
only a small portion of the total CK pool. Consequently,
centaury roots showed higher IAA content as well as IAA/
free CK base ratios compared to shoots. Centaury tissues
also showed increased levels of ‘‘stress hormones’’. In
contrast to SA, considerably higher levels of ABA were
found in centaury shoots than in roots. Our results could
serve as a basis for understanding and elucidating spontaneous
de novo shoot organogenesis and further plant
regeneration of C. erythraea in vitro.
T2  - Journal of Plant Growth Regulation
T1  - Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro
IS  - 2
VL  - 35
DO  - 10.1007/s00344-015-9558-x
SP  - 543
EP  - 552
ER  - 

@article{
author = "Trifunović-Momčilov, Milana and Motyka, Václav and Dragićević, Ivana C. and Petrić, Marija and Jevremović, Slađana and Malbeck, Jiří and Holik, Josef and Dobrev, Petre I. and Subotić, Angelina",
year = "2016",
abstract = "Phytohormones are important regulators of
numerous developmental and physiological processes in
plants. Spontaneous morphogenesis of the common centaury
(Centaurium erythraea Rafn.) is possible on nutrition
medium without addition of any plant growth
regulator depending solely on endogenous phytohormone
levels. Thus, this plant species represents a very good
model system for the investigation of numerous physiological
processes under phytohormonal control in vitro.
We analysed the total amount of endogenous cytokinins
(CKs) including the contents of their individual groups in
shoots and roots of C. erythraea plants grown in vitro. The
total amount of endogenous CKs was 1.4 times higher in
shoots than in roots. Inactive or weakly active N-glucosides
found to predominate in both organs of centaury
plants, whereas free bases and O-glucosides represented
only a small portion of the total CK pool. Consequently,
centaury roots showed higher IAA content as well as IAA/
free CK base ratios compared to shoots. Centaury tissues
also showed increased levels of ‘‘stress hormones’’. In
contrast to SA, considerably higher levels of ABA were
found in centaury shoots than in roots. Our results could
serve as a basis for understanding and elucidating spontaneous
de novo shoot organogenesis and further plant
regeneration of C. erythraea in vitro.",
journal = "Journal of Plant Growth Regulation",
title = "Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro",
number = "2",
volume = "35",
doi = "10.1007/s00344-015-9558-x",
pages = "543-552"
}

Trifunović-Momčilov, M., Motyka, V., Dragićević, I. C., Petrić, M., Jevremović, S., Malbeck, J., Holik, J., Dobrev, P. I.,& Subotić, A.. (2016). Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro. in Journal of Plant Growth Regulation, 35(2), 543-552.
https://doi.org/10.1007/s00344-015-9558-x

Trifunović-Momčilov M, Motyka V, Dragićević IC, Petrić M, Jevremović S, Malbeck J, Holik J, Dobrev PI, Subotić A. Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro. in Journal of Plant Growth Regulation. 2016;35(2):543-552.
doi:10.1007/s00344-015-9558-x .

Trifunović-Momčilov, Milana, Motyka, Václav, Dragićević, Ivana C., Petrić, Marija, Jevremović, Slađana, Malbeck, Jiří, Holik, Josef, Dobrev, Petre I., Subotić, Angelina, "Endogenous Phytohormones in Spontaneously Regenerated Centaurium erythraea Rafn. Plants Grown In Vitro" in Journal of Plant Growth Regulation, 35, no. 2 (2016):543-552,
https://doi.org/10.1007/s00344-015-9558-x . .