TY  - JOUR
AU  - Markelić, Milica
AU  - Mojić, Marija
AU  - Bovan, Dijana
AU  - Jelača, Sanja
AU  - Jović, Zorana
AU  - Purić, Milica
AU  - Koruga, Djuro
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5470
AB  - In our recent study, we showed that in vitro treatment of melanoma cells with hyperpolarized light (HPL) as well as with the second derivative of fullerene, hyper-harmonized hydroxylated fullerene water complex (3HFWC) reduced viability of cells by decreasing their proliferative capacity and inducing senescence and reprogramming towards a normal, melanocytic phenotype. Therefore, we wanted to determine whether these effects persisted in vivo in the syngeneic mouse melanoma model with a combined treatment of HPL irradiation and 3HFWC per os. Our results demonstrated the potent antitumor effects of 3HFWC nanosubstance assisted by HPL irradiation. These effects were primarily driven by the stimulation of melanoma cell growth arrest, the establishment of a senescent phenotype, and melanocytic differentiation on the one hand, and the awakening of the antitumor immune response on the other. In addition, the combined treatment reduced the protumorigenic activity of immune cells by depleting T regulatory cells, myeloid-derived suppressors, and M2 macrophages. The support of the 3HFWC substance by HPL irradiation may be the axis of the new approach design based on tumor cell reprogramming synchronized with the mobilization of the host’s protective immune response.
PB  - Basel: MDPI
T2  - Nanomaterials
T1  - Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo
IS  - 3
VL  - 13
DO  - 10.3390/nano13030372
SP  - 372
ER  - 

@article{
author = "Markelić, Milica and Mojić, Marija and Bovan, Dijana and Jelača, Sanja and Jović, Zorana and Purić, Milica and Koruga, Djuro and Mijatović, Sanja and Maksimović-Ivanić, Danijela",
year = "2023",
abstract = "In our recent study, we showed that in vitro treatment of melanoma cells with hyperpolarized light (HPL) as well as with the second derivative of fullerene, hyper-harmonized hydroxylated fullerene water complex (3HFWC) reduced viability of cells by decreasing their proliferative capacity and inducing senescence and reprogramming towards a normal, melanocytic phenotype. Therefore, we wanted to determine whether these effects persisted in vivo in the syngeneic mouse melanoma model with a combined treatment of HPL irradiation and 3HFWC per os. Our results demonstrated the potent antitumor effects of 3HFWC nanosubstance assisted by HPL irradiation. These effects were primarily driven by the stimulation of melanoma cell growth arrest, the establishment of a senescent phenotype, and melanocytic differentiation on the one hand, and the awakening of the antitumor immune response on the other. In addition, the combined treatment reduced the protumorigenic activity of immune cells by depleting T regulatory cells, myeloid-derived suppressors, and M2 macrophages. The support of the 3HFWC substance by HPL irradiation may be the axis of the new approach design based on tumor cell reprogramming synchronized with the mobilization of the host’s protective immune response.",
publisher = "Basel: MDPI",
journal = "Nanomaterials",
title = "Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo",
number = "3",
volume = "13",
doi = "10.3390/nano13030372",
pages = "372"
}

Markelić, M., Mojić, M., Bovan, D., Jelača, S., Jović, Z., Purić, M., Koruga, D., Mijatović, S.,& Maksimović-Ivanić, D.. (2023). Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo. in Nanomaterials
Basel: MDPI., 13(3), 372.
https://doi.org/10.3390/nano13030372

Markelić M, Mojić M, Bovan D, Jelača S, Jović Z, Purić M, Koruga D, Mijatović S, Maksimović-Ivanić D. Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo. in Nanomaterials. 2023;13(3):372.
doi:10.3390/nano13030372 .

Markelić, Milica, Mojić, Marija, Bovan, Dijana, Jelača, Sanja, Jović, Zorana, Purić, Milica, Koruga, Djuro, Mijatović, Sanja, Maksimović-Ivanić, Danijela, "Melanoma Cell Reprogramming and Awakening of Antitumor Immunity as a Fingerprint of Hyper-Harmonized Hydroxylated Fullerene Water Complex (3HFWC) and Hyperpolarized Light Application In Vivo" in Nanomaterials, 13, no. 3 (2023):372,
https://doi.org/10.3390/nano13030372 . .

TY  - JOUR
AU  - Markelić, Milica
AU  - Drača, Dijana
AU  - Krajnović, Tamara
AU  - Jović, Zorana
AU  - Vuksanović, Milica
AU  - Koruga, Djuro
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4953
AB  - (1) Background: Their unique structure and electron deficiency have brought fullerenes
into the focus of research in many fields, including medicine. The hyper-harmonized hydroxylated
fullerene water complex (3HFWC) formulation has solved the limitations of the poor solubility
and bioavailability of fullerenes. To achieve better antitumor activity, 3HFWC was combined with
short-term irradiation of cells with hyperpolarized light (HPL) generated by the application of a
nanophotonic fullerene filter in a Bioptron® device. The benefits of HPL were confirmed in the
microcirculation, wound healing and immunological function. (2) Methods: B16, B16-F10 and A375
melanoma cells were exposed to a wide spectrum of 3HFWC doses and to a single short-term HPL
irradiation. (3) Results: Apart from the differences in the redox status and level of invasiveness, the
effects of the treatments were quite similar. Decreased viability, morphological alteration, signs of
melanocytic differentiation and cellular senescence were observed upon the successful internalization
of the nanoquantum substance. (4) Conclusions: Overall, 3HFWC/HPL promoted melanoma cell
reprogramming toward a normal phenotype.
PB  - Basel: MDPI
T2  - Nanomaterials (Basel)
T1  - Combined Action of Hyper-Harmonized Hydroxylated Fullerene Water Complex and Hyperpolarized Light Leads to Melanoma Cell Reprogramming In Vitro
IS  - 8
VL  - 12
DO  - 10.3390/nano12081331
SP  - 1331
ER  - 

@article{
author = "Markelić, Milica and Drača, Dijana and Krajnović, Tamara and Jović, Zorana and Vuksanović, Milica and Koruga, Djuro and Mijatović, Sanja and Maksimović-Ivanić, Danijela",
year = "2022",
abstract = "(1) Background: Their unique structure and electron deficiency have brought fullerenes
into the focus of research in many fields, including medicine. The hyper-harmonized hydroxylated
fullerene water complex (3HFWC) formulation has solved the limitations of the poor solubility
and bioavailability of fullerenes. To achieve better antitumor activity, 3HFWC was combined with
short-term irradiation of cells with hyperpolarized light (HPL) generated by the application of a
nanophotonic fullerene filter in a Bioptron® device. The benefits of HPL were confirmed in the
microcirculation, wound healing and immunological function. (2) Methods: B16, B16-F10 and A375
melanoma cells were exposed to a wide spectrum of 3HFWC doses and to a single short-term HPL
irradiation. (3) Results: Apart from the differences in the redox status and level of invasiveness, the
effects of the treatments were quite similar. Decreased viability, morphological alteration, signs of
melanocytic differentiation and cellular senescence were observed upon the successful internalization
of the nanoquantum substance. (4) Conclusions: Overall, 3HFWC/HPL promoted melanoma cell
reprogramming toward a normal phenotype.",
publisher = "Basel: MDPI",
journal = "Nanomaterials (Basel)",
title = "Combined Action of Hyper-Harmonized Hydroxylated Fullerene Water Complex and Hyperpolarized Light Leads to Melanoma Cell Reprogramming In Vitro",
number = "8",
volume = "12",
doi = "10.3390/nano12081331",
pages = "1331"
}

Markelić, M., Drača, D., Krajnović, T., Jović, Z., Vuksanović, M., Koruga, D., Mijatović, S.,& Maksimović-Ivanić, D.. (2022). Combined Action of Hyper-Harmonized Hydroxylated Fullerene Water Complex and Hyperpolarized Light Leads to Melanoma Cell Reprogramming In Vitro. in Nanomaterials (Basel)
Basel: MDPI., 12(8), 1331.
https://doi.org/10.3390/nano12081331

Markelić M, Drača D, Krajnović T, Jović Z, Vuksanović M, Koruga D, Mijatović S, Maksimović-Ivanić D. Combined Action of Hyper-Harmonized Hydroxylated Fullerene Water Complex and Hyperpolarized Light Leads to Melanoma Cell Reprogramming In Vitro. in Nanomaterials (Basel). 2022;12(8):1331.
doi:10.3390/nano12081331 .

Markelić, Milica, Drača, Dijana, Krajnović, Tamara, Jović, Zorana, Vuksanović, Milica, Koruga, Djuro, Mijatović, Sanja, Maksimović-Ivanić, Danijela, "Combined Action of Hyper-Harmonized Hydroxylated Fullerene Water Complex and Hyperpolarized Light Leads to Melanoma Cell Reprogramming In Vitro" in Nanomaterials (Basel), 12, no. 8 (2022):1331,
https://doi.org/10.3390/nano12081331 . .

TY  - CONF
AU  - Drača, Dijana
AU  - Markelić, Milica
AU  - Mojić, Marija
AU  - Jelača, Sanja
AU  - Mijatović, Sanja
AU  - Jović, Zorana
AU  - Dragičević, Aleksandra
AU  - Koruga, Đuro
AU  - Maksimović-Ivanić, Danijela
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4433
UR  - https://onlinelibrary.wiley.com/toc/15214141/2021/51/S1
AB  - Although paucigranulocytic asthma (PGA) is the most common phenotype of stable asthma, its features are not adequately studied. In this study, we aim to display the characteristics 
of PGA. 116 non‐smoker adult asthma patients (80% female, mean age 39± 12.9) admitted to three tertiary centres were included. Their demographic and clinical features, allergy 
status, biochemical results, Asthma Control Test (ACT) scores, spirometry and exhaled nitric oxide (FeNO) measurements were obtained. Induced sputum cytometry was performed. 
According to induced sputum cell counts, four phenotypes were detected: eosinophilic (EA) (22.4%), mixed (MGA) (6.9%), neutrophilic (NA) (7.8%) and paucigranulocytic (62.9%). In 
sputum, macrophages were higher in the PGA group compared to other groups (PGA vs NA and PGA vs MGA: p<0.001, PGA vs EA: p=0.03). The atopy rate between phenotypes was 
the same. Although forced expiratory flow 1st second (FEV1) was similar in four groups, FEV1/FVC was higher (p=0.013) and FEV1 reversibility was lower in PGA patients than the 
corresponding values in other phenotypes (p=0.015). Low reversibility was comparable in both inhaled corticosteroid naive PGA patients and in those on ICS treatment. Although 
insignificant, FeNO and blood eosinophil counts were higher in MGA and EA groups while these were the lowest in the PGA group. Uncontrolled asthma ratio was low in PGA (16%) 
while it was 11% for NA, 25% for MG and 23% in EA. Macrophages are predominant in sputum of PGA patients. Besides lower uncontrolled asthma ratio, lower FEV1 reversibility is 
a prominent characteristic of this phenotype.
PB  - Wiley‐VCH GmbH
C3  - 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
T1  - Anti‐melanoma effects of Hyper‐harmonized hydroxylated fullerene water complex and hyperpolarized light in vivo
IS  - Suppl 1
VL  - 51
DO  - 10.1002/eji.202170200
SP  - 353
ER  - 

@conference{
author = "Drača, Dijana and Markelić, Milica and Mojić, Marija and Jelača, Sanja and Mijatović, Sanja and Jović, Zorana and Dragičević, Aleksandra and Koruga, Đuro and Maksimović-Ivanić, Danijela",
year = "2021",
abstract = "Although paucigranulocytic asthma (PGA) is the most common phenotype of stable asthma, its features are not adequately studied. In this study, we aim to display the characteristics 
of PGA. 116 non‐smoker adult asthma patients (80% female, mean age 39± 12.9) admitted to three tertiary centres were included. Their demographic and clinical features, allergy 
status, biochemical results, Asthma Control Test (ACT) scores, spirometry and exhaled nitric oxide (FeNO) measurements were obtained. Induced sputum cytometry was performed. 
According to induced sputum cell counts, four phenotypes were detected: eosinophilic (EA) (22.4%), mixed (MGA) (6.9%), neutrophilic (NA) (7.8%) and paucigranulocytic (62.9%). In 
sputum, macrophages were higher in the PGA group compared to other groups (PGA vs NA and PGA vs MGA: p<0.001, PGA vs EA: p=0.03). The atopy rate between phenotypes was 
the same. Although forced expiratory flow 1st second (FEV1) was similar in four groups, FEV1/FVC was higher (p=0.013) and FEV1 reversibility was lower in PGA patients than the 
corresponding values in other phenotypes (p=0.015). Low reversibility was comparable in both inhaled corticosteroid naive PGA patients and in those on ICS treatment. Although 
insignificant, FeNO and blood eosinophil counts were higher in MGA and EA groups while these were the lowest in the PGA group. Uncontrolled asthma ratio was low in PGA (16%) 
while it was 11% for NA, 25% for MG and 23% in EA. Macrophages are predominant in sputum of PGA patients. Besides lower uncontrolled asthma ratio, lower FEV1 reversibility is 
a prominent characteristic of this phenotype.",
publisher = "Wiley‐VCH GmbH",
journal = "6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting",
title = "Anti‐melanoma effects of Hyper‐harmonized hydroxylated fullerene water complex and hyperpolarized light in vivo",
number = "Suppl 1",
volume = "51",
doi = "10.1002/eji.202170200",
pages = "353"
}

Drača, D., Markelić, M., Mojić, M., Jelača, S., Mijatović, S., Jović, Z., Dragičević, A., Koruga, Đ.,& Maksimović-Ivanić, D.. (2021). Anti‐melanoma effects of Hyper‐harmonized hydroxylated fullerene water complex and hyperpolarized light in vivo. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting
Wiley‐VCH GmbH., 51(Suppl 1), 353.
https://doi.org/10.1002/eji.202170200

Drača D, Markelić M, Mojić M, Jelača S, Mijatović S, Jović Z, Dragičević A, Koruga Đ, Maksimović-Ivanić D. Anti‐melanoma effects of Hyper‐harmonized hydroxylated fullerene water complex and hyperpolarized light in vivo. in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting. 2021;51(Suppl 1):353.
doi:10.1002/eji.202170200 .

Drača, Dijana, Markelić, Milica, Mojić, Marija, Jelača, Sanja, Mijatović, Sanja, Jović, Zorana, Dragičević, Aleksandra, Koruga, Đuro, Maksimović-Ivanić, Danijela, "Anti‐melanoma effects of Hyper‐harmonized hydroxylated fullerene water complex and hyperpolarized light in vivo" in 6th European Congress of Immunology; 2021 Sep 1-4; Virtual Meeting, 51, no. Suppl 1 (2021):353,
https://doi.org/10.1002/eji.202170200 . .