TY  - JOUR
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Bjelobaba, Ivana
AU  - Dačić, Sanja
AU  - Božić, Iva D.
AU  - Parabučki, Ana B.
AU  - Nedeljković, Nadezda N
AU  - Peković, Sanja
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1167
AB  - Experimental autoimmune encephalomyelitis (EAE) is an animal model of CNS inflammatory and demyelinating disease multiple sclerosis. Microglia and astrocytes represent two related cell types involved in the brain pathology in EAE. Accumulations of hypertrophic reactive astrocytes, intensely stained with glial fibrillary acidic protein (GFAP), which also expressed vimentin, are prominent features of EAE lesions. Recent studies from our laboratory reported that ribavirin attenuated the disease process in EAE by reducing clinical and histological manifestations. EAE was induced in genetically susceptible Dark Agouti rats with syngeneic spinal cord homogenate in complete Freund's adjuvant. Real time PCR and immunohistochemistry were used for determination of GFAP and vimentin gene and tissue expression. We have observed the increased gene and tissue expression of GFAP and vimentin in EAE rats. Ribavirin treatment significantly decreased the number of reactive astrocytes at the peak of disease. At the end of the disease, we have observed reactive GFAP(+) and vimentin(+) astrocytes in both immunized and ribavirin-treated groups, accompanied by increased level of GFAP mRNA. The present study indicates that ribavirin may have the ability to attenuate astrocyte proliferation and glial scaring at the peak of the disease and modulate the astroglial response to EAE during the time-course of the disease.
T2  - Journal of Pharmacological Sciences
T1  - The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis
IS  - 3
VL  - 119
EP  - 232
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1167
ER  - 

@article{
author = "Lavrnja, Irena and Savić, Danijela and Bjelobaba, Ivana and Dačić, Sanja and Božić, Iva D. and Parabučki, Ana B. and Nedeljković, Nadezda N and Peković, Sanja and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2012",
abstract = "Experimental autoimmune encephalomyelitis (EAE) is an animal model of CNS inflammatory and demyelinating disease multiple sclerosis. Microglia and astrocytes represent two related cell types involved in the brain pathology in EAE. Accumulations of hypertrophic reactive astrocytes, intensely stained with glial fibrillary acidic protein (GFAP), which also expressed vimentin, are prominent features of EAE lesions. Recent studies from our laboratory reported that ribavirin attenuated the disease process in EAE by reducing clinical and histological manifestations. EAE was induced in genetically susceptible Dark Agouti rats with syngeneic spinal cord homogenate in complete Freund's adjuvant. Real time PCR and immunohistochemistry were used for determination of GFAP and vimentin gene and tissue expression. We have observed the increased gene and tissue expression of GFAP and vimentin in EAE rats. Ribavirin treatment significantly decreased the number of reactive astrocytes at the peak of disease. At the end of the disease, we have observed reactive GFAP(+) and vimentin(+) astrocytes in both immunized and ribavirin-treated groups, accompanied by increased level of GFAP mRNA. The present study indicates that ribavirin may have the ability to attenuate astrocyte proliferation and glial scaring at the peak of the disease and modulate the astroglial response to EAE during the time-course of the disease.",
journal = "Journal of Pharmacological Sciences",
title = "The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis",
number = "3",
volume = "119",
pages = "232",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1167"
}

Lavrnja, I., Savić, D., Bjelobaba, I., Dačić, S., Božić, I. D., Parabučki, A. B., Nedeljković, N. N., Peković, S., Rakić, L.,& Stojiljković, M. B.. (2012). The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis. in Journal of Pharmacological Sciences, 119(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1167

Lavrnja I, Savić D, Bjelobaba I, Dačić S, Božić ID, Parabučki AB, Nedeljković NN, Peković S, Rakić L, Stojiljković MB. The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis. in Journal of Pharmacological Sciences. 2012;119(3):null-232.
https://hdl.handle.net/21.15107/rcub_ibiss_1167 .

Lavrnja, Irena, Savić, Danijela, Bjelobaba, Ivana, Dačić, Sanja, Božić, Iva D., Parabučki, Ana B., Nedeljković, Nadezda N, Peković, Sanja, Rakić, Ljubisav, Stojiljković, Mirjana B, "The Effect of Ribavirin on Reactive Astrogliosis in Experimental Autoimmune Encephalomyelitis" in Journal of Pharmacological Sciences, 119, no. 3 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1167 .

TY  - JOUR
AU  - Parabučki, Ana B.
AU  - Božić, Iva D.
AU  - Bjelobaba, Ivana
AU  - Lavrnja, Irena
AU  - Brkić, Predrag D
AU  - Jovanović, Tomislav S
AU  - Savić, Danijela
AU  - Stojiljković, Mirjana B
AU  - Peković, Sanja
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1072
AB  - Aim To evaluate the effect of hyperbaric oxygen therapy (HBOT) on superoxide dismutase 2 (SOD2) expression pattern after the cortical stab injury (CSI). Methods CSI was performed on 88 male Wistar rats, divided into control, sham, lesioned, and HBO groups. HBOT protocol was the following: pressure applied was 2.5 absolute atmospheres, for 60 minutes, once a day for consecutive 3 or 10 days. The pattern of SOD2 expression and cellular localization was analyzed using real-time polymerase chain reaction, Western blot, and double-label fluorescence immunohistochemistry. Neurons undergoing degeneration were visualized with Fluoro-Jade (R) B. Results CSI induced significant transient increase in SOD2 protein levels at day 3 post injury, which was followed by a reduction toward control levels at post-injury day 10. At the same time points, mRNA levels for SOD2 in the injured cortex were down-regulated. Exposure to HBO for 3 days considerably down-regulated SOD2 protein levels in the injured cortex, while after 10 days of HBOT an up-regulation of SOD2 was observed. HBOT significantly increased mRNA levels for SOD2 at both time points compared to the corresponding L group, but they were still lower than in controls. Double immunofluorescence staining revealed that 3 days after CSI, up-regulation of SOD2 was mostly due to an increased expression in reactive astrocytes surrounding the lesion site. HBOT attenuated SOD2 expression both in neuronal and astroglial cells. Fluoro-Jade (R) B labeling showed that HBOT significantly decreased the number of degenerating neurons in the injured cortex. Conclusion HBOT alters SOD2 protein and mRNA levels after brain injury in a time-dependent manner.
T2  - Croatian Medical Journal
T1  - Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats
IS  - 6
VL  - 53
SP  - 217
EP  - 597
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1072
ER  - 

@article{
author = "Parabučki, Ana B. and Božić, Iva D. and Bjelobaba, Ivana and Lavrnja, Irena and Brkić, Predrag D and Jovanović, Tomislav S and Savić, Danijela and Stojiljković, Mirjana B and Peković, Sanja",
year = "2012",
abstract = "Aim To evaluate the effect of hyperbaric oxygen therapy (HBOT) on superoxide dismutase 2 (SOD2) expression pattern after the cortical stab injury (CSI). Methods CSI was performed on 88 male Wistar rats, divided into control, sham, lesioned, and HBO groups. HBOT protocol was the following: pressure applied was 2.5 absolute atmospheres, for 60 minutes, once a day for consecutive 3 or 10 days. The pattern of SOD2 expression and cellular localization was analyzed using real-time polymerase chain reaction, Western blot, and double-label fluorescence immunohistochemistry. Neurons undergoing degeneration were visualized with Fluoro-Jade (R) B. Results CSI induced significant transient increase in SOD2 protein levels at day 3 post injury, which was followed by a reduction toward control levels at post-injury day 10. At the same time points, mRNA levels for SOD2 in the injured cortex were down-regulated. Exposure to HBO for 3 days considerably down-regulated SOD2 protein levels in the injured cortex, while after 10 days of HBOT an up-regulation of SOD2 was observed. HBOT significantly increased mRNA levels for SOD2 at both time points compared to the corresponding L group, but they were still lower than in controls. Double immunofluorescence staining revealed that 3 days after CSI, up-regulation of SOD2 was mostly due to an increased expression in reactive astrocytes surrounding the lesion site. HBOT attenuated SOD2 expression both in neuronal and astroglial cells. Fluoro-Jade (R) B labeling showed that HBOT significantly decreased the number of degenerating neurons in the injured cortex. Conclusion HBOT alters SOD2 protein and mRNA levels after brain injury in a time-dependent manner.",
journal = "Croatian Medical Journal",
title = "Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats",
number = "6",
volume = "53",
pages = "217-597",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1072"
}

Parabučki, A. B., Božić, I. D., Bjelobaba, I., Lavrnja, I., Brkić, P. D., Jovanović, T. S., Savić, D., Stojiljković, M. B.,& Peković, S.. (2012). Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats. in Croatian Medical Journal, 53(6), 217-597.
https://hdl.handle.net/21.15107/rcub_ibiss_1072

Parabučki AB, Božić ID, Bjelobaba I, Lavrnja I, Brkić PD, Jovanović TS, Savić D, Stojiljković MB, Peković S. Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats. in Croatian Medical Journal. 2012;53(6):217-597.
https://hdl.handle.net/21.15107/rcub_ibiss_1072 .

Parabučki, Ana B., Božić, Iva D., Bjelobaba, Ivana, Lavrnja, Irena, Brkić, Predrag D, Jovanović, Tomislav S, Savić, Danijela, Stojiljković, Mirjana B, Peković, Sanja, "Hyperbaric oxygenation alters temporal expression pattern of superoxide dismutase 2 after cortical stab injury in rats" in Croatian Medical Journal, 53, no. 6 (2012):217-597,
https://hdl.handle.net/21.15107/rcub_ibiss_1072 .

TY  - CONF
AU  - Dačić, Sanja
AU  - Stojiljković, Mirjana B
AU  - Lavrnja, Irena
AU  - Savić, Danijela
AU  - Parabučki, Ana B.
AU  - Božić, Iva D.
AU  - Bjelobaba, Ivana
AU  - Laketa, Danijela
AU  - Nedeljković, Nadezda N
AU  - Rakić, Ljubisa M
AU  - Peković, Sanja
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1262
C3  - Glia
T1  - Treatment With Combination of B Vitamins Reduces Neurocan Expression After Cortical Ablation
IS  - null
VL  - 59
EP  - S52
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1262
ER  - 

@conference{
author = "Dačić, Sanja and Stojiljković, Mirjana B and Lavrnja, Irena and Savić, Danijela and Parabučki, Ana B. and Božić, Iva D. and Bjelobaba, Ivana and Laketa, Danijela and Nedeljković, Nadezda N and Rakić, Ljubisa M and Peković, Sanja",
year = "2011",
journal = "Glia",
title = "Treatment With Combination of B Vitamins Reduces Neurocan Expression After Cortical Ablation",
number = "null",
volume = "59",
pages = "S52",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1262"
}

Dačić, S., Stojiljković, M. B., Lavrnja, I., Savić, D., Parabučki, A. B., Božić, I. D., Bjelobaba, I., Laketa, D., Nedeljković, N. N., Rakić, L. M.,& Peković, S.. (2011). Treatment With Combination of B Vitamins Reduces Neurocan Expression After Cortical Ablation. in Glia, 59(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1262

Dačić S, Stojiljković MB, Lavrnja I, Savić D, Parabučki AB, Božić ID, Bjelobaba I, Laketa D, Nedeljković NN, Rakić LM, Peković S. Treatment With Combination of B Vitamins Reduces Neurocan Expression After Cortical Ablation. in Glia. 2011;59(null):null-S52.
https://hdl.handle.net/21.15107/rcub_ibiss_1262 .

Dačić, Sanja, Stojiljković, Mirjana B, Lavrnja, Irena, Savić, Danijela, Parabučki, Ana B., Božić, Iva D., Bjelobaba, Ivana, Laketa, Danijela, Nedeljković, Nadezda N, Rakić, Ljubisa M, Peković, Sanja, "Treatment With Combination of B Vitamins Reduces Neurocan Expression After Cortical Ablation" in Glia, 59, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1262 .

TY  - CONF
AU  - Božić, Iva D.
AU  - Gađanski, Ivana
AU  - Lavrnja, Irena
AU  - Parabučki, Ana B.
AU  - Dačić, Sanja
AU  - Bjelobaba, Ivana
AU  - Savić, Danijela
AU  - Rakić, Ljubisa M
AU  - Stojiljković, Mirjana B
AU  - Peković, Sanja
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1261
C3  - Glia
T1  - Temporal and Cellular Expression Pattern of Lc and N Type of Voltage-Dependent Calcium Channels After Cortical Injury
IS  - null
VL  - 59
EP  - S101
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1261
ER  - 

@conference{
author = "Božić, Iva D. and Gađanski, Ivana and Lavrnja, Irena and Parabučki, Ana B. and Dačić, Sanja and Bjelobaba, Ivana and Savić, Danijela and Rakić, Ljubisa M and Stojiljković, Mirjana B and Peković, Sanja",
year = "2011",
journal = "Glia",
title = "Temporal and Cellular Expression Pattern of Lc and N Type of Voltage-Dependent Calcium Channels After Cortical Injury",
number = "null",
volume = "59",
pages = "S101",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1261"
}

Božić, I. D., Gađanski, I., Lavrnja, I., Parabučki, A. B., Dačić, S., Bjelobaba, I., Savić, D., Rakić, L. M., Stojiljković, M. B.,& Peković, S.. (2011). Temporal and Cellular Expression Pattern of Lc and N Type of Voltage-Dependent Calcium Channels After Cortical Injury. in Glia, 59(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1261

Božić ID, Gađanski I, Lavrnja I, Parabučki AB, Dačić S, Bjelobaba I, Savić D, Rakić LM, Stojiljković MB, Peković S. Temporal and Cellular Expression Pattern of Lc and N Type of Voltage-Dependent Calcium Channels After Cortical Injury. in Glia. 2011;59(null):null-S101.
https://hdl.handle.net/21.15107/rcub_ibiss_1261 .

Božić, Iva D., Gađanski, Ivana, Lavrnja, Irena, Parabučki, Ana B., Dačić, Sanja, Bjelobaba, Ivana, Savić, Danijela, Rakić, Ljubisa M, Stojiljković, Mirjana B, Peković, Sanja, "Temporal and Cellular Expression Pattern of Lc and N Type of Voltage-Dependent Calcium Channels After Cortical Injury" in Glia, 59, no. null (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1261 .