TY  - JOUR
AU  - Božić, Iva
AU  - Savić, Danijela
AU  - Jovanović, Marija
AU  - Bjelobaba, Ivana
AU  - Laketa, Danijela
AU  - Nedeljković, Nadežda
AU  - Stojiljković, Mirjana
AU  - Peković, Sanja
AU  - Lavrnja, Irena
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2047
AB  - Microglia play a key role in defending central nervous system from
   various internal and external threats. However, their excessive and/or
   chronic activation is associated with deleterious effects in a variety
   of neurodegenerative diseases. Previously, we have shown that ribavirin
   when applied in clinically relevant dosage (10 mu M) modulates activated
   microglia in complex fashion inducing both anti-and proinflammatory
   effects, simultaneously causing cytotoxicity. Here, we examined
   potential of low-dose ribavirin (0.1 and 1 mu M) to modulate activated
   BV-2 microglia. Morphological and functional activation of BV-2 cells
   was achieved with lipopolysaccharide (LPS) stimulation. Our results
   demonstrated that low-dose ribavirin did not induce cell death, while 10
   mu M ribavirin promoted LPS induced apoptosis. We determined that 1 mu M
   ribavirin was equally efficient in deactivation of LPS induced
   morphological changes as 10 mu M ribavirin treatment. Ribavirin showed
   halfway success in reducing markers of functional activation of
   microglia. Namely, none of the doses had effect on LPS triggered
   production of proinflammatory cytokine tumor necrosis factor alpha. On
   the other hand, low-dose ribavirin proved its effectiveness in reduction
   of another inflammatory mediator, nitric oxide, by inhibiting inducible
   form of nitric oxide synthase. Our results imply that low-dose ribavirin
   may alleviate nitrosative stress during neuroinflammation.
T2  - Analytical Cellular Pathology
T1  - Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of
 BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase
IS  - 923614
DO  - 10.1155/2015/923614
ER  - 

@article{
author = "Božić, Iva and Savić, Danijela and Jovanović, Marija and Bjelobaba, Ivana and Laketa, Danijela and Nedeljković, Nadežda and Stojiljković, Mirjana and Peković, Sanja and Lavrnja, Irena",
year = "2015",
abstract = "Microglia play a key role in defending central nervous system from
   various internal and external threats. However, their excessive and/or
   chronic activation is associated with deleterious effects in a variety
   of neurodegenerative diseases. Previously, we have shown that ribavirin
   when applied in clinically relevant dosage (10 mu M) modulates activated
   microglia in complex fashion inducing both anti-and proinflammatory
   effects, simultaneously causing cytotoxicity. Here, we examined
   potential of low-dose ribavirin (0.1 and 1 mu M) to modulate activated
   BV-2 microglia. Morphological and functional activation of BV-2 cells
   was achieved with lipopolysaccharide (LPS) stimulation. Our results
   demonstrated that low-dose ribavirin did not induce cell death, while 10
   mu M ribavirin promoted LPS induced apoptosis. We determined that 1 mu M
   ribavirin was equally efficient in deactivation of LPS induced
   morphological changes as 10 mu M ribavirin treatment. Ribavirin showed
   halfway success in reducing markers of functional activation of
   microglia. Namely, none of the doses had effect on LPS triggered
   production of proinflammatory cytokine tumor necrosis factor alpha. On
   the other hand, low-dose ribavirin proved its effectiveness in reduction
   of another inflammatory mediator, nitric oxide, by inhibiting inducible
   form of nitric oxide synthase. Our results imply that low-dose ribavirin
   may alleviate nitrosative stress during neuroinflammation.",
journal = "Analytical Cellular Pathology",
title = "Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of
 BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase",
number = "923614",
doi = "10.1155/2015/923614"
}

Božić, I., Savić, D., Jovanović, M., Bjelobaba, I., Laketa, D., Nedeljković, N., Stojiljković, M., Peković, S.,& Lavrnja, I.. (2015). Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of
 BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase. in Analytical Cellular Pathology(923614).
https://doi.org/10.1155/2015/923614

Božić I, Savić D, Jovanović M, Bjelobaba I, Laketa D, Nedeljković N, Stojiljković M, Peković S, Lavrnja I. Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of
 BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase. in Analytical Cellular Pathology. 2015;(923614).
doi:10.1155/2015/923614 .

Božić, Iva, Savić, Danijela, Jovanović, Marija, Bjelobaba, Ivana, Laketa, Danijela, Nedeljković, Nadežda, Stojiljković, Mirjana, Peković, Sanja, Lavrnja, Irena, "Low-Dose Ribavirin Treatments Attenuate Neuroinflammatory Activation of
 BV-2 Cells by Interfering with Inducible Nitric Oxide Synthase" in Analytical Cellular Pathology, no. 923614 (2015),
https://doi.org/10.1155/2015/923614 . .