TY  - JOUR
AU  - Pantović, Aleksandar C
AU  - Krstić, Aleksandra D
AU  - Janjetović, Kristina
AU  - Kocić, Jelena S
AU  - Harhaji-Trajković, Ljubica
AU  - Bugarski, Diana S
AU  - Trajković, Vladimir S
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1064
AB  - We investigated the role of AMP-activated protein kinase (AMPK), Akt, mammalian target of rapamycin (mTOR), autophagy and their interplay in osteogenic differentiation of human dental pulp mesenchymal stem cells. The activation of various members of AMPK, Akt and mTOR signaling pathways and autophagy was analyzed by immunoblotting, while osteogenic differentiation was assessed by alkaline phosphatase staining and real-time RT-PCR/immunoblot quantification of osteocalcin, Runt-related transcription factor 2 and bone morphogenetic protein 2 mRNA and/or protein levels. Osteogenic differentiation of mesenchymal stem cells was associated with early (day 1) activation of AMPK and its target Raptor, coinciding with the inhibition of mTOR and its substrate p70S6 kinase. The early induction of autophagy was demonstrated by accumulation of autophagosome-bound LC3-11, upregulation of proautophagic,beclin-1 and a decrease in the selective autophagic target p62. This was followed by the late activation of Akt/mTOR at days 3-7 of differentiation. The RNA interference-mediated silencing of AMPK, mTOR or autophagy-essential LC3 beta, as well as the pharmacological inhibitors of AMPK (compound C), Akt (10-DEBC hydrochloride), mTOR (rapamycin) and autophagy (bafilomycin A1, chloroquine and ammonium chloride), each suppressed mesenchymal stem cell differentiation to osteoblasts. AMPK knockdown prevented early mTOR inhibition and autophagy induction, as well as late activation of Akt/mTOR signaling, while Ala inhibition suppressed mTOR activation without affecting AMPK phosphorylation. Our data indicate that AMPK controls osteogenic differentiation of human mesenchymal stem cells through both early mTOR inhibition-mediated autophagy and late activation of Akt/mTOR signaling axis. (C) 2012 Elsevier Inc. All rights reserved.
T2  - Bone
T1  - Coordinated time-dependent modulation of AMPK/Akt/mTOR signaling and autophagy controls osteogenic differentiation of human mesenchymal stem cells
IS  - 1
VL  - 52
DO  - 10.1016/j.bone.2012.10.024
SP  - 537
EP  - 531
ER  - 

@article{
author = "Pantović, Aleksandar C and Krstić, Aleksandra D and Janjetović, Kristina and Kocić, Jelena S and Harhaji-Trajković, Ljubica and Bugarski, Diana S and Trajković, Vladimir S",
year = "2013",
abstract = "We investigated the role of AMP-activated protein kinase (AMPK), Akt, mammalian target of rapamycin (mTOR), autophagy and their interplay in osteogenic differentiation of human dental pulp mesenchymal stem cells. The activation of various members of AMPK, Akt and mTOR signaling pathways and autophagy was analyzed by immunoblotting, while osteogenic differentiation was assessed by alkaline phosphatase staining and real-time RT-PCR/immunoblot quantification of osteocalcin, Runt-related transcription factor 2 and bone morphogenetic protein 2 mRNA and/or protein levels. Osteogenic differentiation of mesenchymal stem cells was associated with early (day 1) activation of AMPK and its target Raptor, coinciding with the inhibition of mTOR and its substrate p70S6 kinase. The early induction of autophagy was demonstrated by accumulation of autophagosome-bound LC3-11, upregulation of proautophagic,beclin-1 and a decrease in the selective autophagic target p62. This was followed by the late activation of Akt/mTOR at days 3-7 of differentiation. The RNA interference-mediated silencing of AMPK, mTOR or autophagy-essential LC3 beta, as well as the pharmacological inhibitors of AMPK (compound C), Akt (10-DEBC hydrochloride), mTOR (rapamycin) and autophagy (bafilomycin A1, chloroquine and ammonium chloride), each suppressed mesenchymal stem cell differentiation to osteoblasts. AMPK knockdown prevented early mTOR inhibition and autophagy induction, as well as late activation of Akt/mTOR signaling, while Ala inhibition suppressed mTOR activation without affecting AMPK phosphorylation. Our data indicate that AMPK controls osteogenic differentiation of human mesenchymal stem cells through both early mTOR inhibition-mediated autophagy and late activation of Akt/mTOR signaling axis. (C) 2012 Elsevier Inc. All rights reserved.",
journal = "Bone",
title = "Coordinated time-dependent modulation of AMPK/Akt/mTOR signaling and autophagy controls osteogenic differentiation of human mesenchymal stem cells",
number = "1",
volume = "52",
doi = "10.1016/j.bone.2012.10.024",
pages = "537-531"
}

Pantović, A. C., Krstić, A. D., Janjetović, K., Kocić, J. S., Harhaji-Trajković, L., Bugarski, D. S.,& Trajković, V. S.. (2013). Coordinated time-dependent modulation of AMPK/Akt/mTOR signaling and autophagy controls osteogenic differentiation of human mesenchymal stem cells. in Bone, 52(1), 537-531.
https://doi.org/10.1016/j.bone.2012.10.024

Pantović AC, Krstić AD, Janjetović K, Kocić JS, Harhaji-Trajković L, Bugarski DS, Trajković VS. Coordinated time-dependent modulation of AMPK/Akt/mTOR signaling and autophagy controls osteogenic differentiation of human mesenchymal stem cells. in Bone. 2013;52(1):537-531.
doi:10.1016/j.bone.2012.10.024 .

Pantović, Aleksandar C, Krstić, Aleksandra D, Janjetović, Kristina, Kocić, Jelena S, Harhaji-Trajković, Ljubica, Bugarski, Diana S, Trajković, Vladimir S, "Coordinated time-dependent modulation of AMPK/Akt/mTOR signaling and autophagy controls osteogenic differentiation of human mesenchymal stem cells" in Bone, 52, no. 1 (2013):537-531,
https://doi.org/10.1016/j.bone.2012.10.024 . .

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Harhaji-Trajković, Ljubica
AU  - Todorović-Marković, Biljana M.
AU  - Kepić, Dejan P.
AU  - Arsikin, Katarina M.
AU  - Jovanović, Svetlana P.
AU  - Pantović, Aleksandar C.
AU  - Dramićanin, Miroslav D.
AU  - Trajković, Vladimir S.
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3590
AB  - The present study compared the photothermal anticancer activity of near-infrared (NIR)-excited graphene nanoparticles and carbon nanotubes (CNT). Despite lower NIR-absorbing capacity, suspension of polyvinylpyrrolidone-coated graphene sheets exposed to NIR radiation (808nm, 2W/cm2) generated more heat than DNA or sodium dodecylbenzenesulfonate-solubilized single-wall CNT under the same conditions. Accordingly, graphene nanoparticles performed significantly better than CNT in inducing photothermal death of U251 human glioma cells in vitro. The superior photothermal sensitivity of graphene sheets could be largely explained by their better dispersivity, which has been supported by a simple calculation taking into account thermodynamic, optical and geometrical properties of the two type of carbon nanoparticles. The mechanisms of graphene-mediated photothermal killing of cancer cells apparently involved oxidative stress and mitochondrial membrane depolarization resulting in mixed apoptotic and necrotic cell death characterized by caspase activation/DNA fragmentation and cell membrane damage, respectively
PB  - Elsevier BV
T2  - Biomaterials
T1  - In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes
IS  - 4
VL  - 32
DO  - 10.1016/j.biomaterials.2010.10.030
SP  - 1121
EP  - 1129
ER  - 

@article{
author = "Marković, Zoran M. and Harhaji-Trajković, Ljubica and Todorović-Marković, Biljana M. and Kepić, Dejan P. and Arsikin, Katarina M. and Jovanović, Svetlana P. and Pantović, Aleksandar C. and Dramićanin, Miroslav D. and Trajković, Vladimir S.",
year = "2011",
abstract = "The present study compared the photothermal anticancer activity of near-infrared (NIR)-excited graphene nanoparticles and carbon nanotubes (CNT). Despite lower NIR-absorbing capacity, suspension of polyvinylpyrrolidone-coated graphene sheets exposed to NIR radiation (808nm, 2W/cm2) generated more heat than DNA or sodium dodecylbenzenesulfonate-solubilized single-wall CNT under the same conditions. Accordingly, graphene nanoparticles performed significantly better than CNT in inducing photothermal death of U251 human glioma cells in vitro. The superior photothermal sensitivity of graphene sheets could be largely explained by their better dispersivity, which has been supported by a simple calculation taking into account thermodynamic, optical and geometrical properties of the two type of carbon nanoparticles. The mechanisms of graphene-mediated photothermal killing of cancer cells apparently involved oxidative stress and mitochondrial membrane depolarization resulting in mixed apoptotic and necrotic cell death characterized by caspase activation/DNA fragmentation and cell membrane damage, respectively",
publisher = "Elsevier BV",
journal = "Biomaterials",
title = "In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes",
number = "4",
volume = "32",
doi = "10.1016/j.biomaterials.2010.10.030",
pages = "1121-1129"
}

Marković, Z. M., Harhaji-Trajković, L., Todorović-Marković, B. M., Kepić, D. P., Arsikin, K. M., Jovanović, S. P., Pantović, A. C., Dramićanin, M. D.,& Trajković, V. S.. (2011). In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes. in Biomaterials
Elsevier BV., 32(4), 1121-1129.
https://doi.org/10.1016/j.biomaterials.2010.10.030

Marković ZM, Harhaji-Trajković L, Todorović-Marković BM, Kepić DP, Arsikin KM, Jovanović SP, Pantović AC, Dramićanin MD, Trajković VS. In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes. in Biomaterials. 2011;32(4):1121-1129.
doi:10.1016/j.biomaterials.2010.10.030 .

Marković, Zoran M., Harhaji-Trajković, Ljubica, Todorović-Marković, Biljana M., Kepić, Dejan P., Arsikin, Katarina M., Jovanović, Svetlana P., Pantović, Aleksandar C., Dramićanin, Miroslav D., Trajković, Vladimir S., "In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes" in Biomaterials, 32, no. 4 (2011):1121-1129,
https://doi.org/10.1016/j.biomaterials.2010.10.030 . .

TY  - JOUR
AU  - Trpković, Andreja
AU  - Todorović-Marković, Biljana M
AU  - Kleut, Duska N
AU  - Misirkić Marjanović, Maja
AU  - Janjetović, Kristina
AU  - Vučićević, Ljubica
AU  - Pantović, Aleksandar C
AU  - Jovanović, Svetlana P
AU  - Dramicanin, Miroslav D
AU  - Marković, Zoran J
AU  - Trajković, Vladimir S
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1140
AB  - The present study investigated the hemolytic properties of fullerene (C-60) nanoparticles prepared by solvent exchange using tetrahydrofuran (nC(60)THF), or by mechanochemically assisted complexation with macrocyclic oligosaccharide gamma-cyclodextrin (nC(60)CDX) or the copolymer ethylene vinyl acetate-ethylene vinyl versatate (nC(60)EVA-EVV). The spectrophotometrical analysis of hemoglobin release revealed that only nC(60)THF, but not nC(60)CDX or nC(60)EVA-EVV, was able to cause lysis of human erythrocytes in a dose-and time-dependent manner. Atomic force microscopy revealed that nC(60)THF-mediated hemolysis was preceded by erythrocyte shrinkage and increase in cell surface roughness. A flow cytometric analysis confirmed a decrease in erythrocyte size and demonstrated a significant increase in reactive oxygen species production in red blood cells exposed to nC(60)THF. The nC(60)THF-triggered hemolytic activity was efficiently reduced by the antioxidants N-acetylcysteine and butylated hydroxyanisole, as well as by serum albumin, the most abundant protein in human blood plasma. These data indicate that nC(60)THF can cause serum albumin-preventable hemolysis through oxidative stress-mediated damage of the erythrocyte membrane.
T2  - Nanotechnology
T1  - Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles
IS  - 37
VL  - 21
SP  - 601
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1140
ER  - 

@article{
author = "Trpković, Andreja and Todorović-Marković, Biljana M and Kleut, Duska N and Misirkić Marjanović, Maja and Janjetović, Kristina and Vučićević, Ljubica and Pantović, Aleksandar C and Jovanović, Svetlana P and Dramicanin, Miroslav D and Marković, Zoran J and Trajković, Vladimir S",
year = "2010",
abstract = "The present study investigated the hemolytic properties of fullerene (C-60) nanoparticles prepared by solvent exchange using tetrahydrofuran (nC(60)THF), or by mechanochemically assisted complexation with macrocyclic oligosaccharide gamma-cyclodextrin (nC(60)CDX) or the copolymer ethylene vinyl acetate-ethylene vinyl versatate (nC(60)EVA-EVV). The spectrophotometrical analysis of hemoglobin release revealed that only nC(60)THF, but not nC(60)CDX or nC(60)EVA-EVV, was able to cause lysis of human erythrocytes in a dose-and time-dependent manner. Atomic force microscopy revealed that nC(60)THF-mediated hemolysis was preceded by erythrocyte shrinkage and increase in cell surface roughness. A flow cytometric analysis confirmed a decrease in erythrocyte size and demonstrated a significant increase in reactive oxygen species production in red blood cells exposed to nC(60)THF. The nC(60)THF-triggered hemolytic activity was efficiently reduced by the antioxidants N-acetylcysteine and butylated hydroxyanisole, as well as by serum albumin, the most abundant protein in human blood plasma. These data indicate that nC(60)THF can cause serum albumin-preventable hemolysis through oxidative stress-mediated damage of the erythrocyte membrane.",
journal = "Nanotechnology",
title = "Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles",
number = "37",
volume = "21",
pages = "601-na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1140"
}

Trpković, A., Todorović-Marković, B. M., Kleut, D. N., Misirkić Marjanović, M., Janjetović, K., Vučićević, L., Pantović, A. C., Jovanović, S. P., Dramicanin, M. D., Marković, Z. J.,& Trajković, V. S.. (2010). Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles. in Nanotechnology, 21(37), 601-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1140

Trpković A, Todorović-Marković BM, Kleut DN, Misirkić Marjanović M, Janjetović K, Vučićević L, Pantović AC, Jovanović SP, Dramicanin MD, Marković ZJ, Trajković VS. Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles. in Nanotechnology. 2010;21(37):601-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1140 .

Trpković, Andreja, Todorović-Marković, Biljana M, Kleut, Duska N, Misirkić Marjanović, Maja, Janjetović, Kristina, Vučićević, Ljubica, Pantović, Aleksandar C, Jovanović, Svetlana P, Dramicanin, Miroslav D, Marković, Zoran J, Trajković, Vladimir S, "Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles" in Nanotechnology, 21, no. 37 (2010):601-na,
https://hdl.handle.net/21.15107/rcub_ibiss_1140 .