TY  - JOUR
AU  - Marković, Zoran M.
AU  - Ristić, Biljana Z.
AU  - Arsikin, Katarina M.
AU  - Klisić, Đorđe G.
AU  - Harhaji-Trajković, Ljubica
AU  - Todorović-Marković, Biljana M.
AU  - Kepić, Dejan P.
AU  - Kravić-Stevović, Tamara K.
AU  - Jovanović, Svetlana P.
AU  - Milenković, Marina M.
AU  - Milivojević, Dusan D.
AU  - Bumbaširević, Vladimir Z.
AU  - Dramićanin, Miroslav D.
AU  - Trajković, Vladimir S.
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3587
AB  - The excellent photoluminescent properties of graphene quantum dots (GQD) makes them suitable candidates for biomedical applications, but their cytotoxicity has not been extensively studied. Here we show that electrochemically produced GQD irradiated with blue light (470. nm, 1. W) generate reactive oxygen species, including singlet oxygen, and kill U251 human glioma cells by causing oxidative stress. The cell death induced by photoexcited GQD displayed morphological and/or biochemical characteristics of both apoptosis (phosphatidylserine externalization, caspase activation, DNA fragmentation) and autophagy (formation of autophagic vesicles, LC3-I/LC3-II conversion, degradation of autophagic target p62). Moreover, a genetic inactivation of autophagy-essential LC3B protein partly abrogated the photodynamic cytotoxicity of GQD. These data indicate potential usefulness of GQD in photodynamic therapy, but also raise concerns about their possible toxicity.
PB  - Elsevier BV
T2  - Biomaterials
T1  - Graphene quantum dots as autophagy-inducing photodynamic agents
IS  - 29
VL  - 33
DO  - 10.1016/j.biomaterials.2012.06.060
SP  - 7084
EP  - 7092
ER  - 

@article{
author = "Marković, Zoran M. and Ristić, Biljana Z. and Arsikin, Katarina M. and Klisić, Đorđe G. and Harhaji-Trajković, Ljubica and Todorović-Marković, Biljana M. and Kepić, Dejan P. and Kravić-Stevović, Tamara K. and Jovanović, Svetlana P. and Milenković, Marina M. and Milivojević, Dusan D. and Bumbaširević, Vladimir Z. and Dramićanin, Miroslav D. and Trajković, Vladimir S.",
year = "2012",
abstract = "The excellent photoluminescent properties of graphene quantum dots (GQD) makes them suitable candidates for biomedical applications, but their cytotoxicity has not been extensively studied. Here we show that electrochemically produced GQD irradiated with blue light (470. nm, 1. W) generate reactive oxygen species, including singlet oxygen, and kill U251 human glioma cells by causing oxidative stress. The cell death induced by photoexcited GQD displayed morphological and/or biochemical characteristics of both apoptosis (phosphatidylserine externalization, caspase activation, DNA fragmentation) and autophagy (formation of autophagic vesicles, LC3-I/LC3-II conversion, degradation of autophagic target p62). Moreover, a genetic inactivation of autophagy-essential LC3B protein partly abrogated the photodynamic cytotoxicity of GQD. These data indicate potential usefulness of GQD in photodynamic therapy, but also raise concerns about their possible toxicity.",
publisher = "Elsevier BV",
journal = "Biomaterials",
title = "Graphene quantum dots as autophagy-inducing photodynamic agents",
number = "29",
volume = "33",
doi = "10.1016/j.biomaterials.2012.06.060",
pages = "7084-7092"
}

Marković, Z. M., Ristić, B. Z., Arsikin, K. M., Klisić, Đ. G., Harhaji-Trajković, L., Todorović-Marković, B. M., Kepić, D. P., Kravić-Stevović, T. K., Jovanović, S. P., Milenković, M. M., Milivojević, D. D., Bumbaširević, V. Z., Dramićanin, M. D.,& Trajković, V. S.. (2012). Graphene quantum dots as autophagy-inducing photodynamic agents. in Biomaterials
Elsevier BV., 33(29), 7084-7092.
https://doi.org/10.1016/j.biomaterials.2012.06.060

Marković ZM, Ristić BZ, Arsikin KM, Klisić ĐG, Harhaji-Trajković L, Todorović-Marković BM, Kepić DP, Kravić-Stevović TK, Jovanović SP, Milenković MM, Milivojević DD, Bumbaširević VZ, Dramićanin MD, Trajković VS. Graphene quantum dots as autophagy-inducing photodynamic agents. in Biomaterials. 2012;33(29):7084-7092.
doi:10.1016/j.biomaterials.2012.06.060 .

Marković, Zoran M., Ristić, Biljana Z., Arsikin, Katarina M., Klisić, Đorđe G., Harhaji-Trajković, Ljubica, Todorović-Marković, Biljana M., Kepić, Dejan P., Kravić-Stevović, Tamara K., Jovanović, Svetlana P., Milenković, Marina M., Milivojević, Dusan D., Bumbaširević, Vladimir Z., Dramićanin, Miroslav D., Trajković, Vladimir S., "Graphene quantum dots as autophagy-inducing photodynamic agents" in Biomaterials, 33, no. 29 (2012):7084-7092,
https://doi.org/10.1016/j.biomaterials.2012.06.060 . .

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Harhaji-Trajković, Ljubica
AU  - Todorović-Marković, Biljana M.
AU  - Kepić, Dejan P.
AU  - Arsikin, Katarina M.
AU  - Jovanović, Svetlana P.
AU  - Pantović, Aleksandar C.
AU  - Dramićanin, Miroslav D.
AU  - Trajković, Vladimir S.
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3590
AB  - The present study compared the photothermal anticancer activity of near-infrared (NIR)-excited graphene nanoparticles and carbon nanotubes (CNT). Despite lower NIR-absorbing capacity, suspension of polyvinylpyrrolidone-coated graphene sheets exposed to NIR radiation (808nm, 2W/cm2) generated more heat than DNA or sodium dodecylbenzenesulfonate-solubilized single-wall CNT under the same conditions. Accordingly, graphene nanoparticles performed significantly better than CNT in inducing photothermal death of U251 human glioma cells in vitro. The superior photothermal sensitivity of graphene sheets could be largely explained by their better dispersivity, which has been supported by a simple calculation taking into account thermodynamic, optical and geometrical properties of the two type of carbon nanoparticles. The mechanisms of graphene-mediated photothermal killing of cancer cells apparently involved oxidative stress and mitochondrial membrane depolarization resulting in mixed apoptotic and necrotic cell death characterized by caspase activation/DNA fragmentation and cell membrane damage, respectively
PB  - Elsevier BV
T2  - Biomaterials
T1  - In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes
IS  - 4
VL  - 32
DO  - 10.1016/j.biomaterials.2010.10.030
SP  - 1121
EP  - 1129
ER  - 

@article{
author = "Marković, Zoran M. and Harhaji-Trajković, Ljubica and Todorović-Marković, Biljana M. and Kepić, Dejan P. and Arsikin, Katarina M. and Jovanović, Svetlana P. and Pantović, Aleksandar C. and Dramićanin, Miroslav D. and Trajković, Vladimir S.",
year = "2011",
abstract = "The present study compared the photothermal anticancer activity of near-infrared (NIR)-excited graphene nanoparticles and carbon nanotubes (CNT). Despite lower NIR-absorbing capacity, suspension of polyvinylpyrrolidone-coated graphene sheets exposed to NIR radiation (808nm, 2W/cm2) generated more heat than DNA or sodium dodecylbenzenesulfonate-solubilized single-wall CNT under the same conditions. Accordingly, graphene nanoparticles performed significantly better than CNT in inducing photothermal death of U251 human glioma cells in vitro. The superior photothermal sensitivity of graphene sheets could be largely explained by their better dispersivity, which has been supported by a simple calculation taking into account thermodynamic, optical and geometrical properties of the two type of carbon nanoparticles. The mechanisms of graphene-mediated photothermal killing of cancer cells apparently involved oxidative stress and mitochondrial membrane depolarization resulting in mixed apoptotic and necrotic cell death characterized by caspase activation/DNA fragmentation and cell membrane damage, respectively",
publisher = "Elsevier BV",
journal = "Biomaterials",
title = "In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes",
number = "4",
volume = "32",
doi = "10.1016/j.biomaterials.2010.10.030",
pages = "1121-1129"
}

Marković, Z. M., Harhaji-Trajković, L., Todorović-Marković, B. M., Kepić, D. P., Arsikin, K. M., Jovanović, S. P., Pantović, A. C., Dramićanin, M. D.,& Trajković, V. S.. (2011). In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes. in Biomaterials
Elsevier BV., 32(4), 1121-1129.
https://doi.org/10.1016/j.biomaterials.2010.10.030

Marković ZM, Harhaji-Trajković L, Todorović-Marković BM, Kepić DP, Arsikin KM, Jovanović SP, Pantović AC, Dramićanin MD, Trajković VS. In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes. in Biomaterials. 2011;32(4):1121-1129.
doi:10.1016/j.biomaterials.2010.10.030 .

Marković, Zoran M., Harhaji-Trajković, Ljubica, Todorović-Marković, Biljana M., Kepić, Dejan P., Arsikin, Katarina M., Jovanović, Svetlana P., Pantović, Aleksandar C., Dramićanin, Miroslav D., Trajković, Vladimir S., "In vitro comparison of the photothermal anticancer activity of graphene nanoparticles and carbon nanotubes" in Biomaterials, 32, no. 4 (2011):1121-1129,
https://doi.org/10.1016/j.biomaterials.2010.10.030 . .

TY  - JOUR
AU  - Trpković, Andreja
AU  - Todorović-Marković, Biljana M
AU  - Kleut, Duska N
AU  - Misirkić Marjanović, Maja
AU  - Janjetović, Kristina
AU  - Vučićević, Ljubica
AU  - Pantović, Aleksandar C
AU  - Jovanović, Svetlana P
AU  - Dramicanin, Miroslav D
AU  - Marković, Zoran J
AU  - Trajković, Vladimir S
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1140
AB  - The present study investigated the hemolytic properties of fullerene (C-60) nanoparticles prepared by solvent exchange using tetrahydrofuran (nC(60)THF), or by mechanochemically assisted complexation with macrocyclic oligosaccharide gamma-cyclodextrin (nC(60)CDX) or the copolymer ethylene vinyl acetate-ethylene vinyl versatate (nC(60)EVA-EVV). The spectrophotometrical analysis of hemoglobin release revealed that only nC(60)THF, but not nC(60)CDX or nC(60)EVA-EVV, was able to cause lysis of human erythrocytes in a dose-and time-dependent manner. Atomic force microscopy revealed that nC(60)THF-mediated hemolysis was preceded by erythrocyte shrinkage and increase in cell surface roughness. A flow cytometric analysis confirmed a decrease in erythrocyte size and demonstrated a significant increase in reactive oxygen species production in red blood cells exposed to nC(60)THF. The nC(60)THF-triggered hemolytic activity was efficiently reduced by the antioxidants N-acetylcysteine and butylated hydroxyanisole, as well as by serum albumin, the most abundant protein in human blood plasma. These data indicate that nC(60)THF can cause serum albumin-preventable hemolysis through oxidative stress-mediated damage of the erythrocyte membrane.
T2  - Nanotechnology
T1  - Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles
IS  - 37
VL  - 21
SP  - 601
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1140
ER  - 

@article{
author = "Trpković, Andreja and Todorović-Marković, Biljana M and Kleut, Duska N and Misirkić Marjanović, Maja and Janjetović, Kristina and Vučićević, Ljubica and Pantović, Aleksandar C and Jovanović, Svetlana P and Dramicanin, Miroslav D and Marković, Zoran J and Trajković, Vladimir S",
year = "2010",
abstract = "The present study investigated the hemolytic properties of fullerene (C-60) nanoparticles prepared by solvent exchange using tetrahydrofuran (nC(60)THF), or by mechanochemically assisted complexation with macrocyclic oligosaccharide gamma-cyclodextrin (nC(60)CDX) or the copolymer ethylene vinyl acetate-ethylene vinyl versatate (nC(60)EVA-EVV). The spectrophotometrical analysis of hemoglobin release revealed that only nC(60)THF, but not nC(60)CDX or nC(60)EVA-EVV, was able to cause lysis of human erythrocytes in a dose-and time-dependent manner. Atomic force microscopy revealed that nC(60)THF-mediated hemolysis was preceded by erythrocyte shrinkage and increase in cell surface roughness. A flow cytometric analysis confirmed a decrease in erythrocyte size and demonstrated a significant increase in reactive oxygen species production in red blood cells exposed to nC(60)THF. The nC(60)THF-triggered hemolytic activity was efficiently reduced by the antioxidants N-acetylcysteine and butylated hydroxyanisole, as well as by serum albumin, the most abundant protein in human blood plasma. These data indicate that nC(60)THF can cause serum albumin-preventable hemolysis through oxidative stress-mediated damage of the erythrocyte membrane.",
journal = "Nanotechnology",
title = "Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles",
number = "37",
volume = "21",
pages = "601-na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1140"
}

Trpković, A., Todorović-Marković, B. M., Kleut, D. N., Misirkić Marjanović, M., Janjetović, K., Vučićević, L., Pantović, A. C., Jovanović, S. P., Dramicanin, M. D., Marković, Z. J.,& Trajković, V. S.. (2010). Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles. in Nanotechnology, 21(37), 601-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1140

Trpković A, Todorović-Marković BM, Kleut DN, Misirkić Marjanović M, Janjetović K, Vučićević L, Pantović AC, Jovanović SP, Dramicanin MD, Marković ZJ, Trajković VS. Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles. in Nanotechnology. 2010;21(37):601-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1140 .

Trpković, Andreja, Todorović-Marković, Biljana M, Kleut, Duska N, Misirkić Marjanović, Maja, Janjetović, Kristina, Vučićević, Ljubica, Pantović, Aleksandar C, Jovanović, Svetlana P, Dramicanin, Miroslav D, Marković, Zoran J, Trajković, Vladimir S, "Oxidative stress-mediated hemolytic activity of solvent exchange-prepared fullerene (C-60) nanoparticles" in Nanotechnology, 21, no. 37 (2010):601-na,
https://hdl.handle.net/21.15107/rcub_ibiss_1140 .