TY  - JOUR
AU  - Starcević, Vladan
AU  - Milošević, Verica
AU  - Brkić, B
AU  - Severs, Walter B
PY  - 2002
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1797
AB  - The somatostatin peptides (SRIH-14, SRIH-28) and their multiple receptors are generally associated with anti-proliferative and anti-secretory actions. This study compared, using standard morphometric measurements and terminal serum LH concentrations, effects of intracerebroventricular (icv) SRIH-14 and SFIH-28 in nanomolar amounts on immunohistochemically identified LH cells in pituitary glands of male rats. Rats received I mug/5 mul of SRIH-14 or SRIH-28 icv on days 1,3, and 5, whereas control rats received only icv saline. Animals were killed 5 days later for serum LH assays. Pituitarys were harvested for PAP immunohistochemistry and morphometry. Morphometric measurements were made by an observer blinded to the treatment group. Histochemically identified LH cells from both SRIH groups appeared smaller, often pycnotic and darkly stained compared to those from saline-treated rats. Both SRIH treatments reduced (p < 0.05) the quantitative morphometric measurements for cell volume, nuclear volume, and relative volume density. Both SRIH treatments also reduced serum LH concentration (p < 0.05), supporting the hypothesis that systemic physiology was altered. Collectively, the data support the opinion that nanomolar amounts of either SRIH peptide, acting on receptors reached from cerebrospinal fluid, exert an anti-secretory effect on LH cells of male rats. Modifications of central SRIH receptors may provide an approach for treatment of male sexual dysfunction and/or be of pathophysiologic significance in these disturbances. (C) 2002 Elsevier Science Inc. All fights reserved.
T2  - Life Sciences
T1  - Somatostatin affects morphology and secretion of pituitary luteinizing hormone (LH) cells in male rats
IS  - 25
VL  - 70
EP  - 3027
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1797
ER  - 

@article{
author = "Starcević, Vladan and Milošević, Verica and Brkić, B and Severs, Walter B",
year = "2002",
abstract = "The somatostatin peptides (SRIH-14, SRIH-28) and their multiple receptors are generally associated with anti-proliferative and anti-secretory actions. This study compared, using standard morphometric measurements and terminal serum LH concentrations, effects of intracerebroventricular (icv) SRIH-14 and SFIH-28 in nanomolar amounts on immunohistochemically identified LH cells in pituitary glands of male rats. Rats received I mug/5 mul of SRIH-14 or SRIH-28 icv on days 1,3, and 5, whereas control rats received only icv saline. Animals were killed 5 days later for serum LH assays. Pituitarys were harvested for PAP immunohistochemistry and morphometry. Morphometric measurements were made by an observer blinded to the treatment group. Histochemically identified LH cells from both SRIH groups appeared smaller, often pycnotic and darkly stained compared to those from saline-treated rats. Both SRIH treatments reduced (p < 0.05) the quantitative morphometric measurements for cell volume, nuclear volume, and relative volume density. Both SRIH treatments also reduced serum LH concentration (p < 0.05), supporting the hypothesis that systemic physiology was altered. Collectively, the data support the opinion that nanomolar amounts of either SRIH peptide, acting on receptors reached from cerebrospinal fluid, exert an anti-secretory effect on LH cells of male rats. Modifications of central SRIH receptors may provide an approach for treatment of male sexual dysfunction and/or be of pathophysiologic significance in these disturbances. (C) 2002 Elsevier Science Inc. All fights reserved.",
journal = "Life Sciences",
title = "Somatostatin affects morphology and secretion of pituitary luteinizing hormone (LH) cells in male rats",
number = "25",
volume = "70",
pages = "3027",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1797"
}

Starcević, V., Milošević, V., Brkić, B.,& Severs, W. B.. (2002). Somatostatin affects morphology and secretion of pituitary luteinizing hormone (LH) cells in male rats. in Life Sciences, 70(25).
https://hdl.handle.net/21.15107/rcub_ibiss_1797

Starcević V, Milošević V, Brkić B, Severs WB. Somatostatin affects morphology and secretion of pituitary luteinizing hormone (LH) cells in male rats. in Life Sciences. 2002;70(25):null-3027.
https://hdl.handle.net/21.15107/rcub_ibiss_1797 .

Starcević, Vladan, Milošević, Verica, Brkić, B, Severs, Walter B, "Somatostatin affects morphology and secretion of pituitary luteinizing hormone (LH) cells in male rats" in Life Sciences, 70, no. 25 (2002),
https://hdl.handle.net/21.15107/rcub_ibiss_1797 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Brkić, B
AU  - Starcević, Vladan
PY  - 2001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1818
AB  - The effects of intracerebraventricular (i.c.v.) administration of somatostatins (SRIH-28 or SRIH-14) on zona glomerulosa (ZG) and zona fasciculata (ZF) of the adrenal cortex and blood concentration of adrenocorticotropic hormone (ACTH), aldosterone, cortisol, Na+ and Kf ions in female rats were studied. Each experimental animal was given three 1.0 mug doses of SRIH-28 or SRIH-14 dissolved in 5.0 muL saline by i.c.v. route every second day. Controls were treated in the same way with an equal volume of saline. The rats were sacrificed by decapitation 5 days after the last SRIH injection. The left adrenal gland of each animal was dissected out and prepared for morphometric analyses. Blood samples were collected for hormone level determinations. SRIH-28 but not SRIH-14, led to a significant reduction of absolute and relative volume of the ZG in comparison with the controls (p < 0.05). Total number and volume of the ZG cells and their nuclei was also significantly reduced in SRIH-28-treated rats (p < 0.05) as compared to the controls. No significant changes in morphometric parameters of ZF were recorded. At the same time, a significant decrease of aldosterone, ACTH and Na+ levels were observed (p < 0.05) only in animals treated with SRIH-28, while cortisol concentration was almost unaltered and that of K+ ion significantly increased in comparison with control values. These results indicate that centrally applied SRIH-28 but not SRIH-14, acts inhibiting both the secretory activity and growth of adrenal ZG cells, while insignificantly affecting ZF cells.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - Morphofunctional characteristics of adrenal cortex in female rats after centrally applied somatostatins
IS  - 2
VL  - 20
EP  - 97
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1818
ER  - 

@article{
author = "Milošević, Verica and Brkić, B and Starcević, Vladan",
year = "2001",
abstract = "The effects of intracerebraventricular (i.c.v.) administration of somatostatins (SRIH-28 or SRIH-14) on zona glomerulosa (ZG) and zona fasciculata (ZF) of the adrenal cortex and blood concentration of adrenocorticotropic hormone (ACTH), aldosterone, cortisol, Na+ and Kf ions in female rats were studied. Each experimental animal was given three 1.0 mug doses of SRIH-28 or SRIH-14 dissolved in 5.0 muL saline by i.c.v. route every second day. Controls were treated in the same way with an equal volume of saline. The rats were sacrificed by decapitation 5 days after the last SRIH injection. The left adrenal gland of each animal was dissected out and prepared for morphometric analyses. Blood samples were collected for hormone level determinations. SRIH-28 but not SRIH-14, led to a significant reduction of absolute and relative volume of the ZG in comparison with the controls (p < 0.05). Total number and volume of the ZG cells and their nuclei was also significantly reduced in SRIH-28-treated rats (p < 0.05) as compared to the controls. No significant changes in morphometric parameters of ZF were recorded. At the same time, a significant decrease of aldosterone, ACTH and Na+ levels were observed (p < 0.05) only in animals treated with SRIH-28, while cortisol concentration was almost unaltered and that of K+ ion significantly increased in comparison with control values. These results indicate that centrally applied SRIH-28 but not SRIH-14, acts inhibiting both the secretory activity and growth of adrenal ZG cells, while insignificantly affecting ZF cells.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "Morphofunctional characteristics of adrenal cortex in female rats after centrally applied somatostatins",
number = "2",
volume = "20",
pages = "97",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1818"
}

Milošević, V., Brkić, B.,& Starcević, V.. (2001). Morphofunctional characteristics of adrenal cortex in female rats after centrally applied somatostatins. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 20(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1818

Milošević V, Brkić B, Starcević V. Morphofunctional characteristics of adrenal cortex in female rats after centrally applied somatostatins. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 2001;20(2):null-97.
https://hdl.handle.net/21.15107/rcub_ibiss_1818 .

Milošević, Verica, Brkić, B, Starcević, Vladan, "Morphofunctional characteristics of adrenal cortex in female rats after centrally applied somatostatins" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 20, no. 2 (2001),
https://hdl.handle.net/21.15107/rcub_ibiss_1818 .

TY  - JOUR
AU  - Starcević, Vladan
AU  - Milošević, Verica
AU  - Brkić, B
AU  - Severs, Walter B
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1839
AB  - Effects of intracerebroventricular (i.c.v.) application of somatostatin (SRIH-14 or SRIH-28) on growth and function of pituitary adrenocorticotropes (ACTH cells) were examined in adult female Wistar rats. Animals were subjected to i.c.v. administration of three 1-mu g doses of SRIH-14 or SRIH-28 dissolved in 5 mu l saline every second day, Controls were treated in the same way with the same volume of saline only, ACTH-producing cells were studied using the peroxidase-anti peroxidase (PAP) immunohistochemical procedure; blood samples were collected for hormone analyses 5 days after the last injection, SRIH-28 treatment decreased (p < 0.05) all morphometric parameters compared to control rats. Volume of ACTH cells decreased by 10%, nuclei by 36% and volume density by 13%, No significant changes (p > 0.05) in these para meters occurred after SRIH-14 treatment. Plasma concentration of ACTH in SRIH-28-treated rats was significantly lower (p < 0.05) than in control rats by 35%. In SRIH-14-treated rats, plasma concentration of ACTH was slightly, but not significantly (p > 0.05) increased by 13% compared to saline treatment. These observations suggest that centrally administered somatostatin-28, but not somatostatin-14, is specifically involved in the control of growth and secretory activity of ACTH cells in female rats, Thus, selective pharmacological manipulation of SRIH-28 receptors reached from CSF may affect ACTH activity without altering actions usually attributed to receptors sensitive to SRIF-14. Copyright (C) 2000 S. Karger AG, Basel.
T2  - Pharmacology
T1  - Effects of centrally applied somatostatin on pituitary adrenocorticotropes in female rats
IS  - 4
VL  - 60
EP  - 207
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1839
ER  - 

@article{
author = "Starcević, Vladan and Milošević, Verica and Brkić, B and Severs, Walter B",
year = "2000",
abstract = "Effects of intracerebroventricular (i.c.v.) application of somatostatin (SRIH-14 or SRIH-28) on growth and function of pituitary adrenocorticotropes (ACTH cells) were examined in adult female Wistar rats. Animals were subjected to i.c.v. administration of three 1-mu g doses of SRIH-14 or SRIH-28 dissolved in 5 mu l saline every second day, Controls were treated in the same way with the same volume of saline only, ACTH-producing cells were studied using the peroxidase-anti peroxidase (PAP) immunohistochemical procedure; blood samples were collected for hormone analyses 5 days after the last injection, SRIH-28 treatment decreased (p < 0.05) all morphometric parameters compared to control rats. Volume of ACTH cells decreased by 10%, nuclei by 36% and volume density by 13%, No significant changes (p > 0.05) in these para meters occurred after SRIH-14 treatment. Plasma concentration of ACTH in SRIH-28-treated rats was significantly lower (p < 0.05) than in control rats by 35%. In SRIH-14-treated rats, plasma concentration of ACTH was slightly, but not significantly (p > 0.05) increased by 13% compared to saline treatment. These observations suggest that centrally administered somatostatin-28, but not somatostatin-14, is specifically involved in the control of growth and secretory activity of ACTH cells in female rats, Thus, selective pharmacological manipulation of SRIH-28 receptors reached from CSF may affect ACTH activity without altering actions usually attributed to receptors sensitive to SRIF-14. Copyright (C) 2000 S. Karger AG, Basel.",
journal = "Pharmacology",
title = "Effects of centrally applied somatostatin on pituitary adrenocorticotropes in female rats",
number = "4",
volume = "60",
pages = "207",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1839"
}

Starcević, V., Milošević, V., Brkić, B.,& Severs, W. B.. (2000). Effects of centrally applied somatostatin on pituitary adrenocorticotropes in female rats. in Pharmacology, 60(4).
https://hdl.handle.net/21.15107/rcub_ibiss_1839

Starcević V, Milošević V, Brkić B, Severs WB. Effects of centrally applied somatostatin on pituitary adrenocorticotropes in female rats. in Pharmacology. 2000;60(4):null-207.
https://hdl.handle.net/21.15107/rcub_ibiss_1839 .

Starcević, Vladan, Milošević, Verica, Brkić, B, Severs, Walter B, "Effects of centrally applied somatostatin on pituitary adrenocorticotropes in female rats" in Pharmacology, 60, no. 4 (2000),
https://hdl.handle.net/21.15107/rcub_ibiss_1839 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Brkić, B
AU  - Velkovski, SD
AU  - Sekulić, Milka I.
AU  - Lovren, Mirjana B.
AU  - Starcević, V
AU  - Severs, Walter B
PY  - 1998
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1859
AB  - This study examined effects of intracerebroventricularly (i.c.v.) administered somatostatin (SRIH-14 and SRIH-28) on growth and function of pituitary somatotropes (GH cells) and lactotropes (PRL cells), Male rats received three i.c.v. injections (1 mu g/5 mu l) of SRIH-14 or SRIH-28 every second day, Blood samples were collected for hormone assays and pituitaries were removed for histological and morphometric evaluation 5 days after the last i.c.v. treatment, Compared to control animals, SRIH treatment decreased (p < 0.05) pituitary weight and all morphometric measurements obtained in GH and PRL cells, Concentrations of serum growth hormone (GH) and prolactin (PRL) in both SRIH-treated groups were also lower (p < 0.05) than in control rats, These findings suggest that centrally administered somatostatin is specifically involved in the control of growth and secretory activity of GH and PRL cells, Thus, pharmacological manipulation of SRIH receptors reached from cerebrospinal fluid may alter systemic effects of GH and PRL.
T2  - Pharmacology
T1  - Morphometric and functional changes of rat pituitary somatotropes and lactotropes after central administration of somatostatin
IS  - 1
VL  - 57
EP  - 34
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1859
ER  - 

@article{
author = "Milošević, Verica and Brkić, B and Velkovski, SD and Sekulić, Milka I. and Lovren, Mirjana B. and Starcević, V and Severs, Walter B",
year = "1998",
abstract = "This study examined effects of intracerebroventricularly (i.c.v.) administered somatostatin (SRIH-14 and SRIH-28) on growth and function of pituitary somatotropes (GH cells) and lactotropes (PRL cells), Male rats received three i.c.v. injections (1 mu g/5 mu l) of SRIH-14 or SRIH-28 every second day, Blood samples were collected for hormone assays and pituitaries were removed for histological and morphometric evaluation 5 days after the last i.c.v. treatment, Compared to control animals, SRIH treatment decreased (p < 0.05) pituitary weight and all morphometric measurements obtained in GH and PRL cells, Concentrations of serum growth hormone (GH) and prolactin (PRL) in both SRIH-treated groups were also lower (p < 0.05) than in control rats, These findings suggest that centrally administered somatostatin is specifically involved in the control of growth and secretory activity of GH and PRL cells, Thus, pharmacological manipulation of SRIH receptors reached from cerebrospinal fluid may alter systemic effects of GH and PRL.",
journal = "Pharmacology",
title = "Morphometric and functional changes of rat pituitary somatotropes and lactotropes after central administration of somatostatin",
number = "1",
volume = "57",
pages = "34",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1859"
}

Milošević, V., Brkić, B., Velkovski, S., Sekulić, M. I., Lovren, M. B., Starcević, V.,& Severs, W. B.. (1998). Morphometric and functional changes of rat pituitary somatotropes and lactotropes after central administration of somatostatin. in Pharmacology, 57(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1859

Milošević V, Brkić B, Velkovski S, Sekulić MI, Lovren MB, Starcević V, Severs WB. Morphometric and functional changes of rat pituitary somatotropes and lactotropes after central administration of somatostatin. in Pharmacology. 1998;57(1):null-34.
https://hdl.handle.net/21.15107/rcub_ibiss_1859 .

Milošević, Verica, Brkić, B, Velkovski, SD, Sekulić, Milka I., Lovren, Mirjana B., Starcević, V, Severs, Walter B, "Morphometric and functional changes of rat pituitary somatotropes and lactotropes after central administration of somatostatin" in Pharmacology, 57, no. 1 (1998),
https://hdl.handle.net/21.15107/rcub_ibiss_1859 .

TY  - JOUR
AU  - Milošević, Verica
AU  - Brkić, B
AU  - Velkovski, S
AU  - Lovren, Mirjana B.
AU  - Sekulić, Milka I.
AU  - Starcević, V
PY  - 1997
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1878
AB  - The effects of the intracerebroventricular (i.c.v.) administration of somatostatin (SRIF-14) on zona glomerulosa (ZG), zona fasciculata (ZF) and zona reticularis (ZR) of the adrenal cortex and an the blood concentrations of adrenocorticotropic hormone (ACTH), prolactin (PRL), growth hormone (GH), aldosterone, cortisol, progesterone and estradiol in male rats were studied. Each experimental animal was given three 1 mu g doses of SRIF-14 dissolved in 5 mu L saline by i.c.v. route every second day. Controls were treated in the same way with the same volume of saline. The rats were sacrificed by decapitation five days after the last SRIF-14 dose. The left adrenal gland of each animal was dissected and prepared for histological and morphometric analyses. Blood samples were collected for hormone level determinations. SRIF-14 led to a significant reduction (p < 0.05) of the absolute volume of the adrenal gland and of the volume of the cortex and ZG in comparison with the controls. Total number and volume of ZG cells and their nuclei was also significantly reduced (p < 0.05) in SRIF-14-treated rats as compared to the controls. There were no significant changes in morphometric parameters of ZF and ZR. At the same time, a significant decrease (p < 0.05) of aldosterone, GH, and PRL levels were observed, while the concentration of ACTH, cortisol, progesterone, and estradiol were almost unaltered in comparison with control values. It can be concluded that centrally applied SRIF-14 inhibits both the secretory activity and growth of adrenal ZG cells only, while insignificantly affecting ZF and ZR cells.
T2  - Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry
T1  - The effects of centrally applied somatostatin on the adrenal cortex of male rats
IS  - 2
VL  - 16
EP  - 94
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1878
ER  - 

@article{
author = "Milošević, Verica and Brkić, B and Velkovski, S and Lovren, Mirjana B. and Sekulić, Milka I. and Starcević, V",
year = "1997",
abstract = "The effects of the intracerebroventricular (i.c.v.) administration of somatostatin (SRIF-14) on zona glomerulosa (ZG), zona fasciculata (ZF) and zona reticularis (ZR) of the adrenal cortex and an the blood concentrations of adrenocorticotropic hormone (ACTH), prolactin (PRL), growth hormone (GH), aldosterone, cortisol, progesterone and estradiol in male rats were studied. Each experimental animal was given three 1 mu g doses of SRIF-14 dissolved in 5 mu L saline by i.c.v. route every second day. Controls were treated in the same way with the same volume of saline. The rats were sacrificed by decapitation five days after the last SRIF-14 dose. The left adrenal gland of each animal was dissected and prepared for histological and morphometric analyses. Blood samples were collected for hormone level determinations. SRIF-14 led to a significant reduction (p < 0.05) of the absolute volume of the adrenal gland and of the volume of the cortex and ZG in comparison with the controls. Total number and volume of ZG cells and their nuclei was also significantly reduced (p < 0.05) in SRIF-14-treated rats as compared to the controls. There were no significant changes in morphometric parameters of ZF and ZR. At the same time, a significant decrease (p < 0.05) of aldosterone, GH, and PRL levels were observed, while the concentration of ACTH, cortisol, progesterone, and estradiol were almost unaltered in comparison with control values. It can be concluded that centrally applied SRIF-14 inhibits both the secretory activity and growth of adrenal ZG cells only, while insignificantly affecting ZF and ZR cells.",
journal = "Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry",
title = "The effects of centrally applied somatostatin on the adrenal cortex of male rats",
number = "2",
volume = "16",
pages = "94",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1878"
}

Milošević, V., Brkić, B., Velkovski, S., Lovren, M. B., Sekulić, M. I.,& Starcević, V.. (1997). The effects of centrally applied somatostatin on the adrenal cortex of male rats. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 16(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1878

Milošević V, Brkić B, Velkovski S, Lovren MB, Sekulić MI, Starcević V. The effects of centrally applied somatostatin on the adrenal cortex of male rats. in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry. 1997;16(2):null-94.
https://hdl.handle.net/21.15107/rcub_ibiss_1878 .

Milošević, Verica, Brkić, B, Velkovski, S, Lovren, Mirjana B., Sekulić, Milka I., Starcević, V, "The effects of centrally applied somatostatin on the adrenal cortex of male rats" in Jugoslovenska Medicinska Biohemija-Yugoslav Medical Biochemistry, 16, no. 2 (1997),
https://hdl.handle.net/21.15107/rcub_ibiss_1878 .