TY  - JOUR
AU  - Savić, Danijela
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Bjelobaba, Ivana
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Jovanović, Saša
AU  - Nedeljković, Nadežda N.
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B.
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1534
AB  - Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS) and the helpful tool in preclinical testing of various substances considered for treatment of this human CNS disease. Ribavirin (R) and tiazofurin (T) are purine nucleoside analogues, with the broad spectrum of anti-viral, anti-tumoral and anti-inflammatory properties. We proposed that combined treatment with RT, administrated during the effector phase of EAE, would attenuate disease severity, both clinically and pathologically. Ribavirin was given daily at a dosage of 30 mg/kg and tiazofurin was given at a dosage of 10 mg/kg every other day for 15 days. We detected amelioration of clinical signs and faster recovery in the RT group compared to the control group. Immunohistochemical analyses revealed that RT treatment decrease the number of T cells, macrophages and microglia. In the controls, we detected reactive type of microglia, while in the RT group we noticed ramified/resting form. Demyelination areas and axonal damage were not recorded in the RT group, in contrast to the control group where multiple areas of demyelination zones and axonal loss were found. RT combination treatment suppresses ongoing EAE, prevents demyelination and axonal loss, and therefore may well be the potential therapy for the treatment of MS. (C) 2007 Elsevier B.V. All rights reserved.
PB  - New York, USA: Springer - Plenum publishers
T2  - Journal of the Neurological Sciences
T1  - Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation
IS  - 1-2
VL  - 267
DO  - 10.1016/j.jns.2007.10.010
SP  - 76
EP  - 85
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1534
ER  - 

@article{
author = "Savić, Danijela and Lavrnja, Irena and Peković, Sanja and Dacić, Sanja and Bjelobaba, Ivana and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Jovanović, Saša and Nedeljković, Nadežda N. and Rakić, Ljubisav and Stojiljković, Mirjana B.",
year = "2008",
abstract = "Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis (MS) and the helpful tool in preclinical testing of various substances considered for treatment of this human CNS disease. Ribavirin (R) and tiazofurin (T) are purine nucleoside analogues, with the broad spectrum of anti-viral, anti-tumoral and anti-inflammatory properties. We proposed that combined treatment with RT, administrated during the effector phase of EAE, would attenuate disease severity, both clinically and pathologically. Ribavirin was given daily at a dosage of 30 mg/kg and tiazofurin was given at a dosage of 10 mg/kg every other day for 15 days. We detected amelioration of clinical signs and faster recovery in the RT group compared to the control group. Immunohistochemical analyses revealed that RT treatment decrease the number of T cells, macrophages and microglia. In the controls, we detected reactive type of microglia, while in the RT group we noticed ramified/resting form. Demyelination areas and axonal damage were not recorded in the RT group, in contrast to the control group where multiple areas of demyelination zones and axonal loss were found. RT combination treatment suppresses ongoing EAE, prevents demyelination and axonal loss, and therefore may well be the potential therapy for the treatment of MS. (C) 2007 Elsevier B.V. All rights reserved.",
publisher = "New York, USA: Springer - Plenum publishers",
journal = "Journal of the Neurological Sciences",
title = "Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation",
number = "1-2",
volume = "267",
doi = "10.1016/j.jns.2007.10.010",
pages = "76-85",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1534"
}

Savić, D., Lavrnja, I., Peković, S., Dacić, S., Bjelobaba, I., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Jovanović, S., Nedeljković, N. N., Rakić, L.,& Stojiljković, M. B.. (2008). Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation. in Journal of the Neurological Sciences
New York, USA: Springer - Plenum publishers., 267(1-2), 76-85.
https://doi.org/10.1016/j.jns.2007.10.010
https://hdl.handle.net/21.15107/rcub_ibiss_1534

Savić D, Lavrnja I, Peković S, Dacić S, Bjelobaba I, Mostarica-Stojković MB, Stošić-Grujičić S, Jovanović S, Nedeljković NN, Rakić L, Stojiljković MB. Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation. in Journal of the Neurological Sciences. 2008;267(1-2):76-85.
doi:10.1016/j.jns.2007.10.010
https://hdl.handle.net/21.15107/rcub_ibiss_1534 .

Savić, Danijela, Lavrnja, Irena, Peković, Sanja, Dacić, Sanja, Bjelobaba, Ivana, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Jovanović, Saša, Nedeljković, Nadežda N., Rakić, Ljubisav, Stojiljković, Mirjana B., "Therapeutic effects of combined treatment with ribavirin and tiazofurin on experimental autoimmune encephalomyelitis development: Clinical and histopathological evaluation" in Journal of the Neurological Sciences, 267, no. 1-2 (2008):76-85,
https://doi.org/10.1016/j.jns.2007.10.010 .,
https://hdl.handle.net/21.15107/rcub_ibiss_1534 .

TY  - CONF
AU  - Savić, Danijela
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Šubašić, Sanja A
AU  - Jovanović, Sasa
AU  - Nikić, Ivana
AU  - Bjelobaba, Ivana
AU  - Mostarica-Stojković, Marija B
AU  - Stošić-Grujičić, Stanislava
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana B
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1636
AB  - Experimental autoimmune encephalomyelitis (EAE) is an animal model
of human disease multiple sclerosis (MS). Clinical signs of EAE are result of
an autoaggressive T-cell response against myelin. We have previously
shown that combined treatment with nucleoside analogues (ribavirin — R
+tiazofurin — T), inosine monophosphate dehydrogenase inhibitors,
ameliorates clinical signs and histological lesions of EAE in susceptible
rats, when they are given preventatively. The aim of this study was to
investigate the effect of combined treatment with R+T, given with the
appearance of first EAE clinical sign, on microglia and astrocytes response.
These cells of the target tissue also participate in an autoimmune process.
The disease was induced in Dark Agouti rats with rat spinal cord
homogenate and had acute monophasic course. Ribavirin and tiazofurin
were given at a dosage of 30 mg/kg/day and 10 mg/kg every other day, for
15 days, respectively. Control group was immunized and treated with saline.
Amelioration of clinical signs and faster recovery was shown in group
treated with combination of R and T in comparison to control group.
Immunohistochemical analysis of the spinal cord tissue isolated after
15 days of combined therapy revealed decrease in vimentin positive cells
and microglia compared to control group. Additionally, morphology of
GFAP positive (glial fibrillary acid protein) cells and microglia indicated to
reactive type of these cells in control group. Results of this study revealed that R and T modulate glial response and have EAE protective effects when
they are given from the onset of disease.
C3  - 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan
T1  - Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis
SP  - 167
EP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1636
ER  - 

@conference{
author = "Savić, Danijela and Lavrnja, Irena and Peković, Sanja and Šubašić, Sanja A and Jovanović, Sasa and Nikić, Ivana and Bjelobaba, Ivana and Mostarica-Stojković, Marija B and Stošić-Grujičić, Stanislava and Rakić, Ljubisav and Stojiljković, Mirjana B",
year = "2006",
abstract = "Experimental autoimmune encephalomyelitis (EAE) is an animal model
of human disease multiple sclerosis (MS). Clinical signs of EAE are result of
an autoaggressive T-cell response against myelin. We have previously
shown that combined treatment with nucleoside analogues (ribavirin — R
+tiazofurin — T), inosine monophosphate dehydrogenase inhibitors,
ameliorates clinical signs and histological lesions of EAE in susceptible
rats, when they are given preventatively. The aim of this study was to
investigate the effect of combined treatment with R+T, given with the
appearance of first EAE clinical sign, on microglia and astrocytes response.
These cells of the target tissue also participate in an autoimmune process.
The disease was induced in Dark Agouti rats with rat spinal cord
homogenate and had acute monophasic course. Ribavirin and tiazofurin
were given at a dosage of 30 mg/kg/day and 10 mg/kg every other day, for
15 days, respectively. Control group was immunized and treated with saline.
Amelioration of clinical signs and faster recovery was shown in group
treated with combination of R and T in comparison to control group.
Immunohistochemical analysis of the spinal cord tissue isolated after
15 days of combined therapy revealed decrease in vimentin positive cells
and microglia compared to control group. Additionally, morphology of
GFAP positive (glial fibrillary acid protein) cells and microglia indicated to
reactive type of these cells in control group. Results of this study revealed that R and T modulate glial response and have EAE protective effects when
they are given from the onset of disease.",
journal = "8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan",
title = "Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis",
pages = "167-168",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1636"
}

Savić, D., Lavrnja, I., Peković, S., Šubašić, S. A., Jovanović, S., Nikić, I., Bjelobaba, I., Mostarica-Stojković, M. B., Stošić-Grujičić, S., Rakić, L.,& Stojiljković, M. B.. (2006). Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis. in 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan, 167-168.
https://hdl.handle.net/21.15107/rcub_ibiss_1636

Savić D, Lavrnja I, Peković S, Šubašić SA, Jovanović S, Nikić I, Bjelobaba I, Mostarica-Stojković MB, Stošić-Grujičić S, Rakić L, Stojiljković MB. Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis. in 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan. 2006;:167-168.
https://hdl.handle.net/21.15107/rcub_ibiss_1636 .

Savić, Danijela, Lavrnja, Irena, Peković, Sanja, Šubašić, Sanja A, Jovanović, Sasa, Nikić, Ivana, Bjelobaba, Ivana, Mostarica-Stojković, Marija B, Stošić-Grujičić, Stanislava, Rakić, Ljubisav, Stojiljković, Mirjana B, "Nucleoside analogues effect on glial response in experimental autoimmune encephalomyelitis" in 8th ISNI Congress; 2006 Oct 15-19; Nagoya, Japan (2006):167-168,
https://hdl.handle.net/21.15107/rcub_ibiss_1636 .