TY  - JOUR
AU  - Andrejević, Marko
AU  - Keckarević Marković, Milica
AU  - Bursać, Biljana
AU  - Mihajlović, Milica
AU  - Tanasić, Vanja
AU  - Kecmanović, Miljana
AU  - Keckarevic, Dušan
PY  - 2019
UR  - http://link.springer.com/10.1007/s12024-019-00096-4
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3289
AB  - Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification. In this study, we present the results of the analysis of a 127 bp long fragment of MT-CYB, amplified using universal primers, variable enough to be used for species identification and discrimination, even in highly degraded animal samples. The total number of analyzed species in this study was 30, including 17 mammalian and 13 bird species. Using a newly created primer pair, we successfully amplified and sequenced the target sequence in almost all tested species. The amplification was incomplete in just two species, and as a result, partial, but still variable sequences, were obtained. Using the target fragment we successfully identified all tested samples. Initial results suggested that the intraspecies genetic diversity of the target region, in all tested species, was low - from 0 to 4.72%. The interspecies genetic diversity of the target region, crucial for successful discrimination, showed relatively high diversity, ranging from 8.36% to 42.52%. Given its short length, the target region should be used for species determination, particularly in samples that are degraded or are low in DNA quantity.
T2  - Forensic Science, Medicine, and Pathology
T1  - Identification of a broad spectrum of mammalian and avian species using the short fragment of the mitochondrially encoded cytochrome b gene.
DO  - 10.1007/s12024-019-00096-4
ER  - 

@article{
author = "Andrejević, Marko and Keckarević Marković, Milica and Bursać, Biljana and Mihajlović, Milica and Tanasić, Vanja and Kecmanović, Miljana and Keckarevic, Dušan",
year = "2019",
abstract = "Mitochondrial DNA (mtDNA), especially the gene for cytochrome b (MT-CYB), has been found to be highly informative for species identification. In this study, we present the results of the analysis of a 127 bp long fragment of MT-CYB, amplified using universal primers, variable enough to be used for species identification and discrimination, even in highly degraded animal samples. The total number of analyzed species in this study was 30, including 17 mammalian and 13 bird species. Using a newly created primer pair, we successfully amplified and sequenced the target sequence in almost all tested species. The amplification was incomplete in just two species, and as a result, partial, but still variable sequences, were obtained. Using the target fragment we successfully identified all tested samples. Initial results suggested that the intraspecies genetic diversity of the target region, in all tested species, was low - from 0 to 4.72%. The interspecies genetic diversity of the target region, crucial for successful discrimination, showed relatively high diversity, ranging from 8.36% to 42.52%. Given its short length, the target region should be used for species determination, particularly in samples that are degraded or are low in DNA quantity.",
journal = "Forensic Science, Medicine, and Pathology",
title = "Identification of a broad spectrum of mammalian and avian species using the short fragment of the mitochondrially encoded cytochrome b gene.",
doi = "10.1007/s12024-019-00096-4"
}

Andrejević, M., Keckarević Marković, M., Bursać, B., Mihajlović, M., Tanasić, V., Kecmanović, M.,& Keckarevic, D.. (2019). Identification of a broad spectrum of mammalian and avian species using the short fragment of the mitochondrially encoded cytochrome b gene.. in Forensic Science, Medicine, and Pathology.
https://doi.org/10.1007/s12024-019-00096-4

Andrejević M, Keckarević Marković M, Bursać B, Mihajlović M, Tanasić V, Kecmanović M, Keckarevic D. Identification of a broad spectrum of mammalian and avian species using the short fragment of the mitochondrially encoded cytochrome b gene.. in Forensic Science, Medicine, and Pathology. 2019;.
doi:10.1007/s12024-019-00096-4 .

Andrejević, Marko, Keckarević Marković, Milica, Bursać, Biljana, Mihajlović, Milica, Tanasić, Vanja, Kecmanović, Miljana, Keckarevic, Dušan, "Identification of a broad spectrum of mammalian and avian species using the short fragment of the mitochondrially encoded cytochrome b gene." in Forensic Science, Medicine, and Pathology (2019),
https://doi.org/10.1007/s12024-019-00096-4 . .