TY  - JOUR
AU  - Oalđe Pavlović, Mariana
AU  - Lunić, Tanja
AU  - Graovac, Stefan
AU  - Mandić, Marija
AU  - Repac, Jelena
AU  - Gašić, Uroš
AU  - Božić Nedeljković, Biljana
AU  - Božić, Bojan
PY  - 2022
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0926669022006835
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5013
AB  - Diabetes mellitus has been recognized as one of the most challenging public health problems in the 21st century. The present study aimed to investigate the antidiabetic potential of 18 selected Lamiaceae representatives on yeast α-glucosidase and further translate experimental data into predicted human α-glucosidase inhibition. Since conventional antidiabetic drugs exhibit numerous unavoidable side effects, plant extracts are being increasingly explored as safer alternatives. Plant extracts were prepared by maceration, characterized by liquid chromatography-mass spectrometry (LC-MS), and subjected to in vitro determination of yeast α-glucosidase inhibitory potential, after which the kinetic study of one of the most potent extracts (Mentha × piperita) was performed. Docking simulations were done on both yeast and human α-glucosidase to explore binding modes and energies of the secondary metabolites identified in the examined extracts. Finally, the dynamical model has been constructed (adjR2 = 0.9885) and applied to explore extracts’ inhibition potential onto the human α-glucosidase. The constructed dynamical model revealed, for the first time, that different extracts of Satureja montana represent highly promising candidates for the prevention/treatment of diabetes mellitus. Therefore, the dynamical model described in this study represents a useful tool and a significant advance in the discovery of novel therapeutics with antidiabetic potential in humans.
PB  - Elsevier B.V.
T2  - Industrial Crops and Products
T1  - Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling
VL  - 186
DO  - 10.1016/j.indcrop.2022.115200
SP  - 115200
ER  - 

@article{
author = "Oalđe Pavlović, Mariana and Lunić, Tanja and Graovac, Stefan and Mandić, Marija and Repac, Jelena and Gašić, Uroš and Božić Nedeljković, Biljana and Božić, Bojan",
year = "2022",
abstract = "Diabetes mellitus has been recognized as one of the most challenging public health problems in the 21st century. The present study aimed to investigate the antidiabetic potential of 18 selected Lamiaceae representatives on yeast α-glucosidase and further translate experimental data into predicted human α-glucosidase inhibition. Since conventional antidiabetic drugs exhibit numerous unavoidable side effects, plant extracts are being increasingly explored as safer alternatives. Plant extracts were prepared by maceration, characterized by liquid chromatography-mass spectrometry (LC-MS), and subjected to in vitro determination of yeast α-glucosidase inhibitory potential, after which the kinetic study of one of the most potent extracts (Mentha × piperita) was performed. Docking simulations were done on both yeast and human α-glucosidase to explore binding modes and energies of the secondary metabolites identified in the examined extracts. Finally, the dynamical model has been constructed (adjR2 = 0.9885) and applied to explore extracts’ inhibition potential onto the human α-glucosidase. The constructed dynamical model revealed, for the first time, that different extracts of Satureja montana represent highly promising candidates for the prevention/treatment of diabetes mellitus. Therefore, the dynamical model described in this study represents a useful tool and a significant advance in the discovery of novel therapeutics with antidiabetic potential in humans.",
publisher = "Elsevier B.V.",
journal = "Industrial Crops and Products",
title = "Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling",
volume = "186",
doi = "10.1016/j.indcrop.2022.115200",
pages = "115200"
}

Oalđe Pavlović, M., Lunić, T., Graovac, S., Mandić, M., Repac, J., Gašić, U., Božić Nedeljković, B.,& Božić, B.. (2022). Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling. in Industrial Crops and Products
Elsevier B.V.., 186, 115200.
https://doi.org/10.1016/j.indcrop.2022.115200

Oalđe Pavlović M, Lunić T, Graovac S, Mandić M, Repac J, Gašić U, Božić Nedeljković B, Božić B. Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling. in Industrial Crops and Products. 2022;186:115200.
doi:10.1016/j.indcrop.2022.115200 .

Oalđe Pavlović, Mariana, Lunić, Tanja, Graovac, Stefan, Mandić, Marija, Repac, Jelena, Gašić, Uroš, Božić Nedeljković, Biljana, Božić, Bojan, "Extracts of selected Lamiaceae species as promising antidiabetics: Chemical profiling, in vitro and in silico approach combined with dynamical modeling" in Industrial Crops and Products, 186 (2022):115200,
https://doi.org/10.1016/j.indcrop.2022.115200 . .

TY  - JOUR
AU  - Ehmedah, Adil
AU  - Nedeljković, Predrag
AU  - Dacić, Sanja
AU  - Repac, Jelena
AU  - Drašković-Pavlović, Biljana
AU  - Vučević, Dragana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/22/5426
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4034
AB  - Peripheral nerve injury (PNI) triggers a complex multi-cellular response involving the injured neurons, Schwann cells (SCs), and immune cells, often resulting in poor functional recovery. The aim of this study was to investigate the effects of the treatment with vitamin B (B1, B2, B3, B5, B6, and B12) complex on the interaction between macrophages and SCs during the recovery period after PNI. Transection of the motor branch of the femoral nerve followed by reconstruction by termino-terminal anastomosis was used as an experimental model. Isolated nerves from the sham (S), operated (O), and operated groups treated with the B vitamins (OT group) were used for immunofluorescence analysis. The obtained data indicated that PNI modulates interactions between macrophages and SCs in a time-dependent manner. The treatment with B vitamins complex promoted the M1-to M2-macrophage polarization and accelerated the transition from the non-myelin to myelin-forming SCs, an indicative of SCs maturation. The effect of B vitamins complex on both cell types was accompanied with an increase in macrophage/SC interactions, all of which correlated with the regeneration of the injured nerve. Clearly, the capacity of B vitamins to modulate macrophages-SCs interaction may be promising for the treatment of PNI.
PB  - NLM (Medline)
T2  - Molecules
T1  - Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury
IS  - 22
VL  - 25
DO  - 10.3390/molecules25225426
SP  - 5426
ER  - 

@article{
author = "Ehmedah, Adil and Nedeljković, Predrag and Dacić, Sanja and Repac, Jelena and Drašković-Pavlović, Biljana and Vučević, Dragana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2020",
abstract = "Peripheral nerve injury (PNI) triggers a complex multi-cellular response involving the injured neurons, Schwann cells (SCs), and immune cells, often resulting in poor functional recovery. The aim of this study was to investigate the effects of the treatment with vitamin B (B1, B2, B3, B5, B6, and B12) complex on the interaction between macrophages and SCs during the recovery period after PNI. Transection of the motor branch of the femoral nerve followed by reconstruction by termino-terminal anastomosis was used as an experimental model. Isolated nerves from the sham (S), operated (O), and operated groups treated with the B vitamins (OT group) were used for immunofluorescence analysis. The obtained data indicated that PNI modulates interactions between macrophages and SCs in a time-dependent manner. The treatment with B vitamins complex promoted the M1-to M2-macrophage polarization and accelerated the transition from the non-myelin to myelin-forming SCs, an indicative of SCs maturation. The effect of B vitamins complex on both cell types was accompanied with an increase in macrophage/SC interactions, all of which correlated with the regeneration of the injured nerve. Clearly, the capacity of B vitamins to modulate macrophages-SCs interaction may be promising for the treatment of PNI.",
publisher = "NLM (Medline)",
journal = "Molecules",
title = "Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury",
number = "22",
volume = "25",
doi = "10.3390/molecules25225426",
pages = "5426"
}

Ehmedah, A., Nedeljković, P., Dacić, S., Repac, J., Drašković-Pavlović, B., Vučević, D., Peković, S.,& Božić Nedeljković, B.. (2020). Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury. in Molecules
NLM (Medline)., 25(22), 5426.
https://doi.org/10.3390/molecules25225426

Ehmedah A, Nedeljković P, Dacić S, Repac J, Drašković-Pavlović B, Vučević D, Peković S, Božić Nedeljković B. Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury. in Molecules. 2020;25(22):5426.
doi:10.3390/molecules25225426 .

Ehmedah, Adil, Nedeljković, Predrag, Dacić, Sanja, Repac, Jelena, Drašković-Pavlović, Biljana, Vučević, Dragana, Peković, Sanja, Božić Nedeljković, Biljana, "Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury" in Molecules, 25, no. 22 (2020):5426,
https://doi.org/10.3390/molecules25225426 . .

TY  - JOUR
AU  - Ehmedah, Adil
AU  - Nedeljković, Predrag
AU  - Dacic, Sanja
AU  - Repac, Jelena
AU  - Drašković Pavlović, Biljana
AU  - Vučević, Dragana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/24/4615
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3578
AB  - Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Molecules (Basel, Switzerland)
T1  - Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.
IS  - 24
VL  - 24
DO  - 10.3390/molecules24244615
SP  - 4615
ER  - 

@article{
author = "Ehmedah, Adil and Nedeljković, Predrag and Dacic, Sanja and Repac, Jelena and Drašković Pavlović, Biljana and Vučević, Dragana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2019",
abstract = "Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Molecules (Basel, Switzerland)",
title = "Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.",
number = "24",
volume = "24",
doi = "10.3390/molecules24244615",
pages = "4615"
}

Ehmedah, A., Nedeljković, P., Dacic, S., Repac, J., Drašković Pavlović, B., Vučević, D., Peković, S.,& Božić Nedeljković, B.. (2019). Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.. in Molecules (Basel, Switzerland)
Multidisciplinary Digital Publishing Institute., 24(24), 4615.
https://doi.org/10.3390/molecules24244615

Ehmedah A, Nedeljković P, Dacic S, Repac J, Drašković Pavlović B, Vučević D, Peković S, Božić Nedeljković B. Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.. in Molecules (Basel, Switzerland). 2019;24(24):4615.
doi:10.3390/molecules24244615 .

Ehmedah, Adil, Nedeljković, Predrag, Dacic, Sanja, Repac, Jelena, Drašković Pavlović, Biljana, Vučević, Dragana, Peković, Sanja, Božić Nedeljković, Biljana, "Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury." in Molecules (Basel, Switzerland), 24, no. 24 (2019):4615,
https://doi.org/10.3390/molecules24244615 . .