TY  - JOUR
AU  - Isaković, Aleksandra J
AU  - Janković, Teodora
AU  - Harhaji-Trajković, Ljubica
AU  - Kostić-Rajačić, Slađana V.
AU  - Nikolić, Zoran M
AU  - Vajs, Vlatka E
AU  - Trajković, Vladimir S
PY  - 2008
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1530
AB  - The present study identifies xanthones gentiakochianin and gentiacaulein as the active principles responsible for the in vitro antiglioma action of ether and methanolic extracts of the plant Gentiana kochiana. Gentiakochianin and gentiacaulein induced cell cycle arrest in G(2)/M and G(0)/G(1) phases, respectively, in both C6 rat glioma and U251 human glioma cell lines. The more efficient antiproliferative action of gentiakochianin was associated with its ability to induce microtubule stabilization in a cell-free assay. Both the xanthones reduced mitochondrial membrane potential and increased the production of reactive oxygen species in glioma cells, but only the effects of gentiakochianin were pronounced enough to cause caspase activation and subsequent apoptotic cell death. The assessment of structure-activity relationship in a series of structurally related xanthones from G. kochiana and Gentianella austriaca revealed dihydroxylation at positions 7, 8 of the xanthonic nucleus as the key structural feature responsible for the ability of gentiakochianin to induce microtubule-associated G(2)/M cell block and apoptotic cell death in glioma cells. (C) 2008 Elsevier Ltd. All rights reserved.
T2  - Bioorganic & Medicinal Chemistry
T1  - Antiglioma action of xanthones from Gentiana kochiana: Mechanistic and structure-activity requirements
IS  - 10
VL  - 16
EP  - 5694
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1530
ER  - 

@article{
author = "Isaković, Aleksandra J and Janković, Teodora and Harhaji-Trajković, Ljubica and Kostić-Rajačić, Slađana V. and Nikolić, Zoran M and Vajs, Vlatka E and Trajković, Vladimir S",
year = "2008",
abstract = "The present study identifies xanthones gentiakochianin and gentiacaulein as the active principles responsible for the in vitro antiglioma action of ether and methanolic extracts of the plant Gentiana kochiana. Gentiakochianin and gentiacaulein induced cell cycle arrest in G(2)/M and G(0)/G(1) phases, respectively, in both C6 rat glioma and U251 human glioma cell lines. The more efficient antiproliferative action of gentiakochianin was associated with its ability to induce microtubule stabilization in a cell-free assay. Both the xanthones reduced mitochondrial membrane potential and increased the production of reactive oxygen species in glioma cells, but only the effects of gentiakochianin were pronounced enough to cause caspase activation and subsequent apoptotic cell death. The assessment of structure-activity relationship in a series of structurally related xanthones from G. kochiana and Gentianella austriaca revealed dihydroxylation at positions 7, 8 of the xanthonic nucleus as the key structural feature responsible for the ability of gentiakochianin to induce microtubule-associated G(2)/M cell block and apoptotic cell death in glioma cells. (C) 2008 Elsevier Ltd. All rights reserved.",
journal = "Bioorganic & Medicinal Chemistry",
title = "Antiglioma action of xanthones from Gentiana kochiana: Mechanistic and structure-activity requirements",
number = "10",
volume = "16",
pages = "5694",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1530"
}

Isaković, A. J., Janković, T., Harhaji-Trajković, L., Kostić-Rajačić, S. V., Nikolić, Z. M., Vajs, V. E.,& Trajković, V. S.. (2008). Antiglioma action of xanthones from Gentiana kochiana: Mechanistic and structure-activity requirements. in Bioorganic & Medicinal Chemistry, 16(10).
https://hdl.handle.net/21.15107/rcub_ibiss_1530

Isaković AJ, Janković T, Harhaji-Trajković L, Kostić-Rajačić SV, Nikolić ZM, Vajs VE, Trajković VS. Antiglioma action of xanthones from Gentiana kochiana: Mechanistic and structure-activity requirements. in Bioorganic & Medicinal Chemistry. 2008;16(10):null-5694.
https://hdl.handle.net/21.15107/rcub_ibiss_1530 .

Isaković, Aleksandra J, Janković, Teodora, Harhaji-Trajković, Ljubica, Kostić-Rajačić, Slađana V., Nikolić, Zoran M, Vajs, Vlatka E, Trajković, Vladimir S, "Antiglioma action of xanthones from Gentiana kochiana: Mechanistic and structure-activity requirements" in Bioorganic & Medicinal Chemistry, 16, no. 10 (2008),
https://hdl.handle.net/21.15107/rcub_ibiss_1530 .

TY  - JOUR
AU  - Stojanović, Ivana D.
AU  - Miljković, Đorđe
AU  - Vucković, Olivera
AU  - Harhaji-Trajković, Ljubica
AU  - Nikolić, Zoran M
AU  - Trajković, Vladimir S
AU  - Mostarica-Stojković, Marija B
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1735
AB  - Taxol is a microtubule-stabilizing agent that has recently been shown effective in the treatment of experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. As astrocytes could modulate central nervous system (CNS) autoimmunity through inducible nitric oxide synthase (iNOS)-mediated production of immunoregulatory free radical nitric oxide (NO), we investigated the effect of taxol on NO synthesis in rat astrocytes. Taxol, either alone or in combination with interferon-gamma, induced NO generation in primary astrocytes and astrocytoma C6 cells in a dose- and time-dependent manner. Accordingly, the drug markedly up-regulated the expression of both iNOS mRNA and protein in astrocytes. The observed effect of taxol was mediated through induction of iNOS transcription factors NF-kappaB and IRF-1, and required the activation of p38 MAP kinase and JNK. Finally, NO release by taxol-stimulated astrocytes was blocked with the microtubule-depolymerizing agent colchicine, suggesting the involvement of a microtubule-stabilizing activity of taxol in the observed effect.
T2  - Cellular and Molecular Life Sciences
T1  - Taxol activates inducible nitric oxide synthase in rat astrocytes: the role of MAP kinases and NF-kappa B
IS  - 10
VL  - 61
EP  - 1175
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1735
ER  - 

@article{
author = "Stojanović, Ivana D. and Miljković, Đorđe and Vucković, Olivera and Harhaji-Trajković, Ljubica and Nikolić, Zoran M and Trajković, Vladimir S and Mostarica-Stojković, Marija B",
year = "2004",
abstract = "Taxol is a microtubule-stabilizing agent that has recently been shown effective in the treatment of experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis. As astrocytes could modulate central nervous system (CNS) autoimmunity through inducible nitric oxide synthase (iNOS)-mediated production of immunoregulatory free radical nitric oxide (NO), we investigated the effect of taxol on NO synthesis in rat astrocytes. Taxol, either alone or in combination with interferon-gamma, induced NO generation in primary astrocytes and astrocytoma C6 cells in a dose- and time-dependent manner. Accordingly, the drug markedly up-regulated the expression of both iNOS mRNA and protein in astrocytes. The observed effect of taxol was mediated through induction of iNOS transcription factors NF-kappaB and IRF-1, and required the activation of p38 MAP kinase and JNK. Finally, NO release by taxol-stimulated astrocytes was blocked with the microtubule-depolymerizing agent colchicine, suggesting the involvement of a microtubule-stabilizing activity of taxol in the observed effect.",
journal = "Cellular and Molecular Life Sciences",
title = "Taxol activates inducible nitric oxide synthase in rat astrocytes: the role of MAP kinases and NF-kappa B",
number = "10",
volume = "61",
pages = "1175",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1735"
}

Stojanović, I. D., Miljković, Đ., Vucković, O., Harhaji-Trajković, L., Nikolić, Z. M., Trajković, V. S.,& Mostarica-Stojković, M. B.. (2004). Taxol activates inducible nitric oxide synthase in rat astrocytes: the role of MAP kinases and NF-kappa B. in Cellular and Molecular Life Sciences, 61(10).
https://hdl.handle.net/21.15107/rcub_ibiss_1735

Stojanović ID, Miljković Đ, Vucković O, Harhaji-Trajković L, Nikolić ZM, Trajković VS, Mostarica-Stojković MB. Taxol activates inducible nitric oxide synthase in rat astrocytes: the role of MAP kinases and NF-kappa B. in Cellular and Molecular Life Sciences. 2004;61(10):null-1175.
https://hdl.handle.net/21.15107/rcub_ibiss_1735 .

Stojanović, Ivana D., Miljković, Đorđe, Vucković, Olivera, Harhaji-Trajković, Ljubica, Nikolić, Zoran M, Trajković, Vladimir S, Mostarica-Stojković, Marija B, "Taxol activates inducible nitric oxide synthase in rat astrocytes: the role of MAP kinases and NF-kappa B" in Cellular and Molecular Life Sciences, 61, no. 10 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1735 .