TY  - JOUR
AU  - Smiljanić, Kosara
AU  - Todorović, Smilja
AU  - Mladenović, Aleksandra
AU  - Vanmierlo, Tim
AU  - Lütjohann, Dieter
AU  - Ivković, Sanja
AU  - Kanazir, Selma
PY  - 2018
UR  - http://link.springer.com/10.1007/s10522-018-9743-y
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29340834
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2960
AB  - Albeit aging is an inevitable process, the rate of aging is susceptible to modifications. Dietary restriction (DR) is a vigorous nongenetic and nonpharmacological intervention that is known to delay aging and increase healthspan in diverse species. This study aimed to compare the impact of different restricting feeding regimes such as limited daily feeding (LDF, 60% AL) and intermittent feeding (IF) on brain energy homeostasis during aging. The analysis was focused on the key molecules in glucose and cholesterol metabolism in the cortex and hippocampus of middle-aged (12-month-old) and aged (24-month-old) male Wistar rats. We measured the impact of different DRs on the expression levels of AMPK, glucose transporters (GLUT1, GLUT3, GLUT4), and the rate-limiting enzyme in the cholesterol synthesis pathway (HMGCR). Additionally, we assessed the changes in the amounts of cholesterol, its metabolite, and precursors following LDF and IF. IF decreased the levels of AMPK and pAMPK in the cortex while the increased levels were detected in the hippocampus. Glucose metabolism was more affected in the cortex, while cholesterol metabolism was more influenced in the hippocampus. Overall, the hippocampus was more resilient to the DRs, with fewer changes compared to the cortex. We showed that LDF and IF differently affected the brain energy homeostasis during aging and that specific brain regions exhibited distinct vulnerabilities towards DRs. Consequently, special attention should be paid to the DR application among elderly as different phases of aging do not respond equally to altered nutritional regimes.
T2  - Biogerontology
T1  - Limited daily feeding and intermittent feeding have different effects on regional brain energy homeostasis during aging.
IS  - 2
VL  - 19
DO  - 10.1007/s10522-018-9743-y
SP  - 121
EP  - 132
ER  - 

@article{
author = "Smiljanić, Kosara and Todorović, Smilja and Mladenović, Aleksandra and Vanmierlo, Tim and Lütjohann, Dieter and Ivković, Sanja and Kanazir, Selma",
year = "2018",
abstract = "Albeit aging is an inevitable process, the rate of aging is susceptible to modifications. Dietary restriction (DR) is a vigorous nongenetic and nonpharmacological intervention that is known to delay aging and increase healthspan in diverse species. This study aimed to compare the impact of different restricting feeding regimes such as limited daily feeding (LDF, 60% AL) and intermittent feeding (IF) on brain energy homeostasis during aging. The analysis was focused on the key molecules in glucose and cholesterol metabolism in the cortex and hippocampus of middle-aged (12-month-old) and aged (24-month-old) male Wistar rats. We measured the impact of different DRs on the expression levels of AMPK, glucose transporters (GLUT1, GLUT3, GLUT4), and the rate-limiting enzyme in the cholesterol synthesis pathway (HMGCR). Additionally, we assessed the changes in the amounts of cholesterol, its metabolite, and precursors following LDF and IF. IF decreased the levels of AMPK and pAMPK in the cortex while the increased levels were detected in the hippocampus. Glucose metabolism was more affected in the cortex, while cholesterol metabolism was more influenced in the hippocampus. Overall, the hippocampus was more resilient to the DRs, with fewer changes compared to the cortex. We showed that LDF and IF differently affected the brain energy homeostasis during aging and that specific brain regions exhibited distinct vulnerabilities towards DRs. Consequently, special attention should be paid to the DR application among elderly as different phases of aging do not respond equally to altered nutritional regimes.",
journal = "Biogerontology",
title = "Limited daily feeding and intermittent feeding have different effects on regional brain energy homeostasis during aging.",
number = "2",
volume = "19",
doi = "10.1007/s10522-018-9743-y",
pages = "121-132"
}

Smiljanić, K., Todorović, S., Mladenović, A., Vanmierlo, T., Lütjohann, D., Ivković, S.,& Kanazir, S.. (2018). Limited daily feeding and intermittent feeding have different effects on regional brain energy homeostasis during aging.. in Biogerontology, 19(2), 121-132.
https://doi.org/10.1007/s10522-018-9743-y

Smiljanić K, Todorović S, Mladenović A, Vanmierlo T, Lütjohann D, Ivković S, Kanazir S. Limited daily feeding and intermittent feeding have different effects on regional brain energy homeostasis during aging.. in Biogerontology. 2018;19(2):121-132.
doi:10.1007/s10522-018-9743-y .

Smiljanić, Kosara, Todorović, Smilja, Mladenović, Aleksandra, Vanmierlo, Tim, Lütjohann, Dieter, Ivković, Sanja, Kanazir, Selma, "Limited daily feeding and intermittent feeding have different effects on regional brain energy homeostasis during aging." in Biogerontology, 19, no. 2 (2018):121-132,
https://doi.org/10.1007/s10522-018-9743-y . .

TY  - JOUR
AU  - Smiljanić, Kosara
AU  - Vanmierlo, Tim
AU  - Mladenović, Aleksandra
AU  - Perović, Milka
AU  - Lončarević-Vasiljković, Nataša
AU  - Tesić, Vesna T
AU  - Rakić, Ljubisav
AU  - Ruždijić, Sabera
AU  - Lutjohann, Dieter
AU  - Kanazir, Selma
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/953
AB  - Disturbance of cholesterol homeostasis in the brain is coupled to age-related brain dysfunction. In the present work, we studied the relationship between aging and cholesterol metabolism in two brain regions, the cortex and hippocampus, as well as in the sera and liver of 6-, 12-, 18- and 24-month-old male Wistar rats. Using gas chromatography-mass spectrometry, we undertook a comparative analysis of the concentrations of cholesterol, its precursors and metabolites, as well as dietary-derived phytosterols. During aging, the concentrations of the three cholesterol precursors examined (lanosterol, lathosterol and desmosterol) were unchanged in the cortex, except for desmosterol which decreased (44 %) in 18-month-old rats. In the hippocampus, aging was associated with a significant reduction in lanosterol and lathosterol concentrations at 24 months (28 and 25 %, respectively), as well as by a significant decrease of desmosterol concentration at 18 and 24 months (36 and 51 %, respectively). In contrast, in the liver we detected age-induced increases in lanosterol and lathosterol concentrations, and no change in desmosterol concentration. The amounts of these sterols were lower than in the brain regions. In the cortex and hippocampus, desmosterol was the predominant cholesterol precursor. In the liver, lathosterol was the most abundant precursor. This ratio remained stable during aging. The most striking effect of aging observed in our study was a significant decrease in desmosterol concentration in the hippocampus which could reflect age-related reduced synaptic plasticity, thus representing one of the detrimental effects of advanced age.
T2  - Lipids
T1  - Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver
IS  - 11
VL  - 48
SP  - 5
EP  - 1077
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_953
ER  - 

@article{
author = "Smiljanić, Kosara and Vanmierlo, Tim and Mladenović, Aleksandra and Perović, Milka and Lončarević-Vasiljković, Nataša and Tesić, Vesna T and Rakić, Ljubisav and Ruždijić, Sabera and Lutjohann, Dieter and Kanazir, Selma",
year = "2013",
abstract = "Disturbance of cholesterol homeostasis in the brain is coupled to age-related brain dysfunction. In the present work, we studied the relationship between aging and cholesterol metabolism in two brain regions, the cortex and hippocampus, as well as in the sera and liver of 6-, 12-, 18- and 24-month-old male Wistar rats. Using gas chromatography-mass spectrometry, we undertook a comparative analysis of the concentrations of cholesterol, its precursors and metabolites, as well as dietary-derived phytosterols. During aging, the concentrations of the three cholesterol precursors examined (lanosterol, lathosterol and desmosterol) were unchanged in the cortex, except for desmosterol which decreased (44 %) in 18-month-old rats. In the hippocampus, aging was associated with a significant reduction in lanosterol and lathosterol concentrations at 24 months (28 and 25 %, respectively), as well as by a significant decrease of desmosterol concentration at 18 and 24 months (36 and 51 %, respectively). In contrast, in the liver we detected age-induced increases in lanosterol and lathosterol concentrations, and no change in desmosterol concentration. The amounts of these sterols were lower than in the brain regions. In the cortex and hippocampus, desmosterol was the predominant cholesterol precursor. In the liver, lathosterol was the most abundant precursor. This ratio remained stable during aging. The most striking effect of aging observed in our study was a significant decrease in desmosterol concentration in the hippocampus which could reflect age-related reduced synaptic plasticity, thus representing one of the detrimental effects of advanced age.",
journal = "Lipids",
title = "Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver",
number = "11",
volume = "48",
pages = "5-1077",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_953"
}

Smiljanić, K., Vanmierlo, T., Mladenović, A., Perović, M., Lončarević-Vasiljković, N., Tesić, V. T., Rakić, L., Ruždijić, S., Lutjohann, D.,& Kanazir, S.. (2013). Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver. in Lipids, 48(11), 5-1077.
https://hdl.handle.net/21.15107/rcub_ibiss_953

Smiljanić K, Vanmierlo T, Mladenović A, Perović M, Lončarević-Vasiljković N, Tesić VT, Rakić L, Ruždijić S, Lutjohann D, Kanazir S. Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver. in Lipids. 2013;48(11):5-1077.
https://hdl.handle.net/21.15107/rcub_ibiss_953 .

Smiljanić, Kosara, Vanmierlo, Tim, Mladenović, Aleksandra, Perović, Milka, Lončarević-Vasiljković, Nataša, Tesić, Vesna T, Rakić, Ljubisav, Ruždijić, Sabera, Lutjohann, Dieter, Kanazir, Selma, "Aging Induces Tissue-Specific Changes in Cholesterol Metabolism in Rat Brain and Liver" in Lipids, 48, no. 11 (2013):5-1077,
https://hdl.handle.net/21.15107/rcub_ibiss_953 .