TY  - JOUR
AU  - Radović, Julijana M
AU  - Maksimović-Ivanić, Danijela
AU  - Timotijević, Gordana S
AU  - Popadić, S
AU  - Ramić, Zorica D.
AU  - Trajković, Vladimir S
AU  - Miljković, Đorđe
AU  - Stošić-Grujičić, Stanislava
AU  - Mijatović, Sanja
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1135
AB  - Intrinsic characteristics of melanoma cells such as expression of inducible nitric oxide synthase (iNOS), redox status, and activity of signaling pathways involved in proliferation, differentiation and cell death define the response of the cells to the diverse treatments. In this context we compared the effectiveness of herbal antaquinone aloe emodin (AE) against mouse B16 melanoma and human A375, different in initial activity of ERK1/2, constitutive iNOS expression and basal level of reactive oxygen species (ROS). Both cell lines are sensitive to AE treatment. However, while the agent induces differentiation of B16 cells toward melanocytes, in A375 cells promoted massive apoptosis. Differentiation of B16 cells, characterized by enhanced melanin production and tyrosinase activity, was mediated by H2O2 production synchronized with rapid p53 accumulation and enhanced expression of cyclins D1 and D3. Caspase mediated apoptosis triggered in A375 cells was accompanied with Bcl-2 but not iNOS down-regulation. In addition, opposite regulation of Akt-ERK1/2 axis in AE treated B16 and A375 cells correlated with different outcome of the treatment. However, AE in a dose-dependent manner rescued both B16 and A375 cells from doxorubicin- or paclitaxel-induced killing. These data indicate that caution is warranted when AE is administrated to the patients with conventional chemotherapy. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Cell-type dependent response of melanoma cells to aloe emodin
IS  - 9
VL  - 50
SP  - 911
EP  - 3189
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1135
ER  - 

@article{
author = "Radović, Julijana M and Maksimović-Ivanić, Danijela and Timotijević, Gordana S and Popadić, S and Ramić, Zorica D. and Trajković, Vladimir S and Miljković, Đorđe and Stošić-Grujičić, Stanislava and Mijatović, Sanja",
year = "2012",
abstract = "Intrinsic characteristics of melanoma cells such as expression of inducible nitric oxide synthase (iNOS), redox status, and activity of signaling pathways involved in proliferation, differentiation and cell death define the response of the cells to the diverse treatments. In this context we compared the effectiveness of herbal antaquinone aloe emodin (AE) against mouse B16 melanoma and human A375, different in initial activity of ERK1/2, constitutive iNOS expression and basal level of reactive oxygen species (ROS). Both cell lines are sensitive to AE treatment. However, while the agent induces differentiation of B16 cells toward melanocytes, in A375 cells promoted massive apoptosis. Differentiation of B16 cells, characterized by enhanced melanin production and tyrosinase activity, was mediated by H2O2 production synchronized with rapid p53 accumulation and enhanced expression of cyclins D1 and D3. Caspase mediated apoptosis triggered in A375 cells was accompanied with Bcl-2 but not iNOS down-regulation. In addition, opposite regulation of Akt-ERK1/2 axis in AE treated B16 and A375 cells correlated with different outcome of the treatment. However, AE in a dose-dependent manner rescued both B16 and A375 cells from doxorubicin- or paclitaxel-induced killing. These data indicate that caution is warranted when AE is administrated to the patients with conventional chemotherapy. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Cell-type dependent response of melanoma cells to aloe emodin",
number = "9",
volume = "50",
pages = "911-3189",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1135"
}

Radović, J. M., Maksimović-Ivanić, D., Timotijević, G. S., Popadić, S., Ramić, Z. D., Trajković, V. S., Miljković, Đ., Stošić-Grujičić, S.,& Mijatović, S.. (2012). Cell-type dependent response of melanoma cells to aloe emodin. in Food and Chemical Toxicology, 50(9), 911-3189.
https://hdl.handle.net/21.15107/rcub_ibiss_1135

Radović JM, Maksimović-Ivanić D, Timotijević GS, Popadić S, Ramić ZD, Trajković VS, Miljković Đ, Stošić-Grujičić S, Mijatović S. Cell-type dependent response of melanoma cells to aloe emodin. in Food and Chemical Toxicology. 2012;50(9):911-3189.
https://hdl.handle.net/21.15107/rcub_ibiss_1135 .

Radović, Julijana M, Maksimović-Ivanić, Danijela, Timotijević, Gordana S, Popadić, S, Ramić, Zorica D., Trajković, Vladimir S, Miljković, Đorđe, Stošić-Grujičić, Stanislava, Mijatović, Sanja, "Cell-type dependent response of melanoma cells to aloe emodin" in Food and Chemical Toxicology, 50, no. 9 (2012):911-3189,
https://hdl.handle.net/21.15107/rcub_ibiss_1135 .

TY  - JOUR
AU  - Mijatović, Sanja
AU  - Timotijević, Gordana S
AU  - Miljković, Đorđe
AU  - Radović, Julijana M
AU  - Maksimović-Ivanić, Danijela
AU  - Dekanski, Dragana P.
AU  - Stošić-Grujičić, Stanislava
PY  - 2011
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1297
AB  - Various constituents of the olive tree (Olea europaea) have been traditionally used in the treatment of infection, inflammation, prevention of chronic diseases, cardiovascular disorders and cancer. The anticancer potential of dry olive leaf extract (DOLE) represents the net effect of multilevel interactions between different biologically active compounds from the extract, cancer cells and conventional therapy. In this context, it was of primary interest to evaluate the influence of DOLE on progression of the highly malignant, immuno-and chemoresistant type of skin cancer-melanoma. DOLE significantly inhibited proliferation and subsequently restricted clonogenicity of the B16 mouse melanoma cell line in vitro. Moreover, late phase tumor treatment with DOLE significantly reduced tumor volume in a syngeneic strain of mice. DOLE-treated B16 cells were blocked in the G(0)/G(1) phase of the cell cycle, underwent early apoptosis and died by late necrosis. At the molecular level, the dying process started as caspase dependent, but finalized as caspase independent. In concordance, overexpression of antiapoptotic members of the Bcl-2 family, Bcl-2 and Bcl-XL, and diminished expression of their natural antagonists, Bim and p53, were observed. Despite molecular suppression of the proapoptotic process, DOLE successfully promoted cell death mainly through disruption of cell membrane integrity and late caspase-independent fragmentation of genetic material. Taken together, the results of this study indicate that DOLE possesses strong antimelanoma potential. When DOLE was applied in combination with different chemotherapeutics, various outcomes, including synergy and antagonism, were observed. This requires caution in the use of the extract as a supplementary antitumor therapeutic.
T2  - International Journal of Cancer
T1  - Multiple antimelanoma potential of dry olive leaf extract
IS  - 8
VL  - 128
EP  - 1965
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1297
ER  - 

@article{
author = "Mijatović, Sanja and Timotijević, Gordana S and Miljković, Đorđe and Radović, Julijana M and Maksimović-Ivanić, Danijela and Dekanski, Dragana P. and Stošić-Grujičić, Stanislava",
year = "2011",
abstract = "Various constituents of the olive tree (Olea europaea) have been traditionally used in the treatment of infection, inflammation, prevention of chronic diseases, cardiovascular disorders and cancer. The anticancer potential of dry olive leaf extract (DOLE) represents the net effect of multilevel interactions between different biologically active compounds from the extract, cancer cells and conventional therapy. In this context, it was of primary interest to evaluate the influence of DOLE on progression of the highly malignant, immuno-and chemoresistant type of skin cancer-melanoma. DOLE significantly inhibited proliferation and subsequently restricted clonogenicity of the B16 mouse melanoma cell line in vitro. Moreover, late phase tumor treatment with DOLE significantly reduced tumor volume in a syngeneic strain of mice. DOLE-treated B16 cells were blocked in the G(0)/G(1) phase of the cell cycle, underwent early apoptosis and died by late necrosis. At the molecular level, the dying process started as caspase dependent, but finalized as caspase independent. In concordance, overexpression of antiapoptotic members of the Bcl-2 family, Bcl-2 and Bcl-XL, and diminished expression of their natural antagonists, Bim and p53, were observed. Despite molecular suppression of the proapoptotic process, DOLE successfully promoted cell death mainly through disruption of cell membrane integrity and late caspase-independent fragmentation of genetic material. Taken together, the results of this study indicate that DOLE possesses strong antimelanoma potential. When DOLE was applied in combination with different chemotherapeutics, various outcomes, including synergy and antagonism, were observed. This requires caution in the use of the extract as a supplementary antitumor therapeutic.",
journal = "International Journal of Cancer",
title = "Multiple antimelanoma potential of dry olive leaf extract",
number = "8",
volume = "128",
pages = "1965",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1297"
}

Mijatović, S., Timotijević, G. S., Miljković, Đ., Radović, J. M., Maksimović-Ivanić, D., Dekanski, D. P.,& Stošić-Grujičić, S.. (2011). Multiple antimelanoma potential of dry olive leaf extract. in International Journal of Cancer, 128(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1297

Mijatović S, Timotijević GS, Miljković Đ, Radović JM, Maksimović-Ivanić D, Dekanski DP, Stošić-Grujičić S. Multiple antimelanoma potential of dry olive leaf extract. in International Journal of Cancer. 2011;128(8):null-1965.
https://hdl.handle.net/21.15107/rcub_ibiss_1297 .

Mijatović, Sanja, Timotijević, Gordana S, Miljković, Đorđe, Radović, Julijana M, Maksimović-Ivanić, Danijela, Dekanski, Dragana P., Stošić-Grujičić, Stanislava, "Multiple antimelanoma potential of dry olive leaf extract" in International Journal of Cancer, 128, no. 8 (2011),
https://hdl.handle.net/21.15107/rcub_ibiss_1297 .

TY  - CONF
AU  - Mijatović, Sanja
AU  - Radović, Julijana M
AU  - Timotijević, Gordana S
AU  - Mojić, Marija
AU  - Miljković, Đorđe
AU  - Dekanski, Dragana P.
AU  - Stošić-Grujičić, Stanislava
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1443
C3  - Planta Medica
T1  - Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway
IS  - 9
VL  - 75
EP  - 903
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1443
ER  - 

@conference{
author = "Mijatović, Sanja and Radović, Julijana M and Timotijević, Gordana S and Mojić, Marija and Miljković, Đorđe and Dekanski, Dragana P. and Stošić-Grujičić, Stanislava",
year = "2009",
journal = "Planta Medica",
title = "Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway",
number = "9",
volume = "75",
pages = "903",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1443"
}

Mijatović, S., Radović, J. M., Timotijević, G. S., Mojić, M., Miljković, Đ., Dekanski, D. P.,& Stošić-Grujičić, S.. (2009). Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway. in Planta Medica, 75(9).
https://hdl.handle.net/21.15107/rcub_ibiss_1443

Mijatović S, Radović JM, Timotijević GS, Mojić M, Miljković Đ, Dekanski DP, Stošić-Grujičić S. Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway. in Planta Medica. 2009;75(9):null-903.
https://hdl.handle.net/21.15107/rcub_ibiss_1443 .

Mijatović, Sanja, Radović, Julijana M, Timotijević, Gordana S, Mojić, Marija, Miljković, Đorđe, Dekanski, Dragana P., Stošić-Grujičić, Stanislava, "Dry olive leaf extract promotes avant-garde apoptosis in melanoma cells; switch from caspase- dependent to caspase- independent pathway" in Planta Medica, 75, no. 9 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1443 .