TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Tucović, Dina
AU  - Demenesku, Jelena
AU  - Subota, Vesna
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2018
UR  - https://www.sciencedirect.com/science/article/pii/S027869151830019X?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2999
AB  - Warfarin is the world's most widely used anticoagulant drug. Its anticoagulant activity is based on the inhibition of the vitamin K-dependent (VKD) step in the complete synthesis of a number of blood coagulation factors that are required for normal blood coagulation. Warfarin also affects synthesis of VKD proteins not related to haemostasis including those involved in bone growth and vascular calcification. Antithrombotic activity of warfarin is considered responsible for some aspects of its anti-tumour activity of warfarin. Some aspects of activities against tumours seem not to be related to haemostasis and included effects of warfarin on non-haemostatic VKD proteins as well as those not related to VKD proteins. Inflammatory/immunomodulatory effects of warfarin indicate much broader potential of action of this drug both in physiological and pathological processes. This review provides an overview of the published data dealing with the effects of warfarin on biological processes other than haemostasis.
PB  - Pergamon
T2  - Food and Chemical Toxicology
T1  - Effects of warfarin on biological processes other than haemostasis: A review.
VL  - 113
DO  - 10.1016/j.fct.2018.01.019
SP  - 19
EP  - 32
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Mirkov, Ivana and Ninkov, Marina and Tucović, Dina and Demenesku, Jelena and Subota, Vesna and Kataranovski, Dragan and Kataranovski, Milena",
year = "2018",
abstract = "Warfarin is the world's most widely used anticoagulant drug. Its anticoagulant activity is based on the inhibition of the vitamin K-dependent (VKD) step in the complete synthesis of a number of blood coagulation factors that are required for normal blood coagulation. Warfarin also affects synthesis of VKD proteins not related to haemostasis including those involved in bone growth and vascular calcification. Antithrombotic activity of warfarin is considered responsible for some aspects of its anti-tumour activity of warfarin. Some aspects of activities against tumours seem not to be related to haemostasis and included effects of warfarin on non-haemostatic VKD proteins as well as those not related to VKD proteins. Inflammatory/immunomodulatory effects of warfarin indicate much broader potential of action of this drug both in physiological and pathological processes. This review provides an overview of the published data dealing with the effects of warfarin on biological processes other than haemostasis.",
publisher = "Pergamon",
journal = "Food and Chemical Toxicology",
title = "Effects of warfarin on biological processes other than haemostasis: A review.",
volume = "113",
doi = "10.1016/j.fct.2018.01.019",
pages = "19-32"
}

Popov Aleksandrov, A., Mirkov, I., Ninkov, M., Tucović, D., Demenesku, J., Subota, V., Kataranovski, D.,& Kataranovski, M.. (2018). Effects of warfarin on biological processes other than haemostasis: A review.. in Food and Chemical Toxicology
Pergamon., 113, 19-32.
https://doi.org/10.1016/j.fct.2018.01.019

Popov Aleksandrov A, Mirkov I, Ninkov M, Tucović D, Demenesku J, Subota V, Kataranovski D, Kataranovski M. Effects of warfarin on biological processes other than haemostasis: A review.. in Food and Chemical Toxicology. 2018;113:19-32.
doi:10.1016/j.fct.2018.01.019 .

Popov Aleksandrov, Aleksandra, Mirkov, Ivana, Ninkov, Marina, Tucović, Dina, Demenesku, Jelena, Subota, Vesna, Kataranovski, Dragan, Kataranovski, Milena, "Effects of warfarin on biological processes other than haemostasis: A review." in Food and Chemical Toxicology, 113 (2018):19-32,
https://doi.org/10.1016/j.fct.2018.01.019 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Subota, Vesna
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2018
UR  - http://doi.wiley.com/10.1111/1749-4877.12296
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29168613
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3021
AB  - Studies of wild animals' immunity often use comparison with laboratory-raised individuals. Using such an approach, various data were obtained concerning wild Norway rat's immunity. Lower or higher potential of immune system cells to respond to activation stimuli were shown, because of analysis of disparate parameters and/ or small number of analyzed individuals. Inconsistent differences between laboratory and wild rats were shown too, owing to great response variability in wild rats. We hypothesized that wild rats will express more intense immune activity compared to their laboratory counterparts which live in a less demanding environment. To test this, we analyzed the circulating levels of inflammatory cytokine interleukin-6 (IL-6), a mediator which has a central role in host immune defense. In addition, we examined the activity of the central immune organ, the spleen, including cell proliferation and production of pro-inflammatory cytokines interferon-γ (IFN-γ) and interleukin-17 (IL-17), which are major effectors of cellular adaptive immune response. In order to obtain reasonable insight into the immunity of wild Norway rats, analysis was conducted on a much larger number of individuals compared to other studies. Higher levels of plasma IL-6, higher spleen mass, cellularity and basal IFN-γ production concomitantly with lower basal production of anti-inflammatory cytokine interleukin-10 (IL-10) revealed more intense immune activity in the wild compared to laboratory rats. However, lower responsiveness of their spleen cells' proinflammatory cytokine production to concanavalin A (ConA) stimulation, along with preserved capacity of IL-10 response, might be perceived as an indication of wild rats' reduced capability to cope with incoming environmental stimuli, but also as a means to limit tissue damage.
T2  - Integrative Zoology
T1  - Immune defense of wild-caught Norway rats is characterized by increased levels of basal activity but reduced capability to respond to further immune stimulation.
IS  - 2
VL  - 13
DO  - 10.1111/1749-4877.12296
SP  - 180
EP  - 193
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Subota, Vesna and Kataranovski, Dragan and Kataranovski, Milena",
year = "2018",
abstract = "Studies of wild animals' immunity often use comparison with laboratory-raised individuals. Using such an approach, various data were obtained concerning wild Norway rat's immunity. Lower or higher potential of immune system cells to respond to activation stimuli were shown, because of analysis of disparate parameters and/ or small number of analyzed individuals. Inconsistent differences between laboratory and wild rats were shown too, owing to great response variability in wild rats. We hypothesized that wild rats will express more intense immune activity compared to their laboratory counterparts which live in a less demanding environment. To test this, we analyzed the circulating levels of inflammatory cytokine interleukin-6 (IL-6), a mediator which has a central role in host immune defense. In addition, we examined the activity of the central immune organ, the spleen, including cell proliferation and production of pro-inflammatory cytokines interferon-γ (IFN-γ) and interleukin-17 (IL-17), which are major effectors of cellular adaptive immune response. In order to obtain reasonable insight into the immunity of wild Norway rats, analysis was conducted on a much larger number of individuals compared to other studies. Higher levels of plasma IL-6, higher spleen mass, cellularity and basal IFN-γ production concomitantly with lower basal production of anti-inflammatory cytokine interleukin-10 (IL-10) revealed more intense immune activity in the wild compared to laboratory rats. However, lower responsiveness of their spleen cells' proinflammatory cytokine production to concanavalin A (ConA) stimulation, along with preserved capacity of IL-10 response, might be perceived as an indication of wild rats' reduced capability to cope with incoming environmental stimuli, but also as a means to limit tissue damage.",
journal = "Integrative Zoology",
title = "Immune defense of wild-caught Norway rats is characterized by increased levels of basal activity but reduced capability to respond to further immune stimulation.",
number = "2",
volume = "13",
doi = "10.1111/1749-4877.12296",
pages = "180-193"
}

Mirkov, I., Popov Aleksandrov, A., Subota, V., Kataranovski, D.,& Kataranovski, M.. (2018). Immune defense of wild-caught Norway rats is characterized by increased levels of basal activity but reduced capability to respond to further immune stimulation.. in Integrative Zoology, 13(2), 180-193.
https://doi.org/10.1111/1749-4877.12296

Mirkov I, Popov Aleksandrov A, Subota V, Kataranovski D, Kataranovski M. Immune defense of wild-caught Norway rats is characterized by increased levels of basal activity but reduced capability to respond to further immune stimulation.. in Integrative Zoology. 2018;13(2):180-193.
doi:10.1111/1749-4877.12296 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Subota, Vesna, Kataranovski, Dragan, Kataranovski, Milena, "Immune defense of wild-caught Norway rats is characterized by increased levels of basal activity but reduced capability to respond to further immune stimulation." in Integrative Zoology, 13, no. 2 (2018):180-193,
https://doi.org/10.1111/1749-4877.12296 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Demenesku, Jelena
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Stefik, Debora
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4812
AB  - Intestinal hemorrhage characterizes effectiveness of warfarin (WF) as rodenticide and is among
adverse effects of therapy in humans. Having in mind genetic variations in the effectiveness of
WF in wild rats and in the doses required for therapeutic effect, strain differences in the intestinal
toxicity of oral warfarin in rats were examined in this study. High WF dose (3.5 mg/l) led to
mortality in Albino Oxford (AO) rats, with no lethality in Dark Agouti (DA) rats. Higher values
of prothrombin time were noted at low WF dose (0.35 mg/l) in the former strain. Leukocyte
infiltration in intestine noted at this dose in both strains was associated with oxidative injury and
more pronounced anti-oxidative response in AO rats. Suppression of mesenteric lymph node cell
proliferation and IFN-γ and IL-10 production in AO rats and lack of these effects in DA rats,
represent different strategies to protect vulnerable intestine from harmful immune responses.
PB  - Amsterdam, Holland:Elsevier B.V.
T2  - Environmental Toxicology and Pharmacology
T1  - Strain differences in intestinal toxicity of warfarin in rats
VL  - 48
DO  - 10.1016/j.etap.2016.10.019
SP  - 175
EP  - 182
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Ninkov, Marina and Mileusnić, Dina and Demenesku, Jelena and Zolotarevski, Lidija and Subota, Vesna and Stefik, Debora and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Intestinal hemorrhage characterizes effectiveness of warfarin (WF) as rodenticide and is among
adverse effects of therapy in humans. Having in mind genetic variations in the effectiveness of
WF in wild rats and in the doses required for therapeutic effect, strain differences in the intestinal
toxicity of oral warfarin in rats were examined in this study. High WF dose (3.5 mg/l) led to
mortality in Albino Oxford (AO) rats, with no lethality in Dark Agouti (DA) rats. Higher values
of prothrombin time were noted at low WF dose (0.35 mg/l) in the former strain. Leukocyte
infiltration in intestine noted at this dose in both strains was associated with oxidative injury and
more pronounced anti-oxidative response in AO rats. Suppression of mesenteric lymph node cell
proliferation and IFN-γ and IL-10 production in AO rats and lack of these effects in DA rats,
represent different strategies to protect vulnerable intestine from harmful immune responses.",
publisher = "Amsterdam, Holland:Elsevier B.V.",
journal = "Environmental Toxicology and Pharmacology",
title = "Strain differences in intestinal toxicity of warfarin in rats",
volume = "48",
doi = "10.1016/j.etap.2016.10.019",
pages = "175-182"
}

Mirkov, I., Popov Aleksandrov, A., Ninkov, M., Mileusnić, D., Demenesku, J., Zolotarevski, L., Subota, V., Stefik, D., Kataranovski, D.,& Kataranovski, M.. (2016). Strain differences in intestinal toxicity of warfarin in rats. in Environmental Toxicology and Pharmacology
Amsterdam, Holland:Elsevier B.V.., 48, 175-182.
https://doi.org/10.1016/j.etap.2016.10.019

Mirkov I, Popov Aleksandrov A, Ninkov M, Mileusnić D, Demenesku J, Zolotarevski L, Subota V, Stefik D, Kataranovski D, Kataranovski M. Strain differences in intestinal toxicity of warfarin in rats. in Environmental Toxicology and Pharmacology. 2016;48:175-182.
doi:10.1016/j.etap.2016.10.019 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Ninkov, Marina, Mileusnić, Dina, Demenesku, Jelena, Zolotarevski, Lidija, Subota, Vesna, Stefik, Debora, Kataranovski, Dragan, Kataranovski, Milena, "Strain differences in intestinal toxicity of warfarin in rats" in Environmental Toxicology and Pharmacology, 48 (2016):175-182,
https://doi.org/10.1016/j.etap.2016.10.019 . .

TY  - JOUR
AU  - Mirkov, Ivana
AU  - Popov Aleksandrov, Aleksandra
AU  - Demenesku, Jelena
AU  - Ninkov, Marina
AU  - Mileusnić, Dina
AU  - Zolotarevski, Lidija
AU  - Subota, Vesna
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4815
AB  - Though warfarin is extensively used in the prevention and treatment of thromboembolic
processes in humans, adverse effects of warfarin therapy have been recognized. Intestinal
hemorrhage is one of the hazards of anticoagulant therapy, but the mechanisms of warfarin
toxicity are virtually unknown. In this work, the effects of 30 days oral warfarin (0.35 mg/l and
3.5 mg/l) intake on rat’s gut were examined. Both doses resulted in prolongation of prothrombin
time. Systemic effects of higher warfarin dose (increases in plasma AST, proteinuria, hematuria,
changes in peripheral blood hematological parameters) were seen. Warfarin intake resulted in
histologically evident tissue damage, leukocyte infiltration and intestinal inflammation [increases
in myeloperoxidase activity, malondialdehyde content, superoxide dismutase and catalase
activity, proinflammatory cytokine (IFN-γ, IL-17) concentrations in intestinal homogenates]. In
contrast, suppression of gut-draining mesenteric lymph node (MLN) cell activity [proliferation
responsiveness, production of IFN-γ and IL-17 to T lymphocyte mitogen Concanavalin A
stimulation] was noted. Inhibition of regulatory cytokine IL-10 production by MLN cells,
suggests commitment of MLN to the suppression of all inflammatory activities and creation of
the microenvironment which is non-permissive for induction of potentially harmful immune
response. These novel findings indicate the need of staying alert for (adverse) effects of warfarin
therapy.
PB  - Amsterdam : Elsevier
T2  - Food and Chemical Toxicology
T1  - Intestinal toxicity of oral warfarin intake in rats
VL  - 94
DO  - 10.1016/j.fct.2016.05.007
SP  - 11
EP  - 18
ER  - 

@article{
author = "Mirkov, Ivana and Popov Aleksandrov, Aleksandra and Demenesku, Jelena and Ninkov, Marina and Mileusnić, Dina and Zolotarevski, Lidija and Subota, Vesna and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "Though warfarin is extensively used in the prevention and treatment of thromboembolic
processes in humans, adverse effects of warfarin therapy have been recognized. Intestinal
hemorrhage is one of the hazards of anticoagulant therapy, but the mechanisms of warfarin
toxicity are virtually unknown. In this work, the effects of 30 days oral warfarin (0.35 mg/l and
3.5 mg/l) intake on rat’s gut were examined. Both doses resulted in prolongation of prothrombin
time. Systemic effects of higher warfarin dose (increases in plasma AST, proteinuria, hematuria,
changes in peripheral blood hematological parameters) were seen. Warfarin intake resulted in
histologically evident tissue damage, leukocyte infiltration and intestinal inflammation [increases
in myeloperoxidase activity, malondialdehyde content, superoxide dismutase and catalase
activity, proinflammatory cytokine (IFN-γ, IL-17) concentrations in intestinal homogenates]. In
contrast, suppression of gut-draining mesenteric lymph node (MLN) cell activity [proliferation
responsiveness, production of IFN-γ and IL-17 to T lymphocyte mitogen Concanavalin A
stimulation] was noted. Inhibition of regulatory cytokine IL-10 production by MLN cells,
suggests commitment of MLN to the suppression of all inflammatory activities and creation of
the microenvironment which is non-permissive for induction of potentially harmful immune
response. These novel findings indicate the need of staying alert for (adverse) effects of warfarin
therapy.",
publisher = "Amsterdam : Elsevier",
journal = "Food and Chemical Toxicology",
title = "Intestinal toxicity of oral warfarin intake in rats",
volume = "94",
doi = "10.1016/j.fct.2016.05.007",
pages = "11-18"
}

Mirkov, I., Popov Aleksandrov, A., Demenesku, J., Ninkov, M., Mileusnić, D., Zolotarevski, L., Subota, V., Kataranovski, D.,& Kataranovski, M.. (2016). Intestinal toxicity of oral warfarin intake in rats. in Food and Chemical Toxicology
Amsterdam : Elsevier., 94, 11-18.
https://doi.org/10.1016/j.fct.2016.05.007

Mirkov I, Popov Aleksandrov A, Demenesku J, Ninkov M, Mileusnić D, Zolotarevski L, Subota V, Kataranovski D, Kataranovski M. Intestinal toxicity of oral warfarin intake in rats. in Food and Chemical Toxicology. 2016;94:11-18.
doi:10.1016/j.fct.2016.05.007 .

Mirkov, Ivana, Popov Aleksandrov, Aleksandra, Demenesku, Jelena, Ninkov, Marina, Mileusnić, Dina, Zolotarevski, Lidija, Subota, Vesna, Kataranovski, Dragan, Kataranovski, Milena, "Intestinal toxicity of oral warfarin intake in rats" in Food and Chemical Toxicology, 94 (2016):11-18,
https://doi.org/10.1016/j.fct.2016.05.007 . .

TY  - JOUR
AU  - Popov Aleksandrov, Aleksandra
AU  - Belij, Sandra
AU  - Subota, Vesna S
AU  - Zolotarevski, Lidija D
AU  - Mirkov, Ivana
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1061
AB  - Warfarin is a Vitamin K (VK) antagonist that affects Vitamin K-dependent (VKD) processes, including blood coagulation, as well as processes unrelated to hemostasis such as bone growth, calcification, and growth of some cell types. In addition, warfarin exerts influence on some non-VKD-related activities, including anti-tumor and immunomodulating activity. With respect to the latter, both immune stimulating and suppressive effects have been noted in different experimental systems. To explore the in vivo immunomodulatory potential of warfarin on one type of activity (i.e., cytokine production) in two different immune cell populations (i.e., mononuclear or polymorphonuclear cells), effects of subchronic oral warfarin intake in rats on pro-inflammatory cytokine (i.e., TNF alpha, IL-6) production by peripheral blood mononuclear and polymorphonuclear cells (granulocytes) was examined. Differential effects of warfarin intake on TNF alpha and IL-6 were noted, depending on the type of peripheral blood leukocytes and on the cytokine examined. Specifically, a lack of effect on TNF alpha and a priming of IL-6 production by mononuclear cells along with a decrease in TNF alpha and a lack of effect on IL-6 in polymorphonuclear cells were seen in warfarin-exposed hosts. The cell-and cytokine-dependent effects from subchronic oral warfarin intake on peripheral blood leukocytes demonstrated in this study could, possibly, differentially affect reactions mediated by these cells. Ultimately, the observed effects in rats might have implications for those humans who are on long-term/prolonged warfarin therapy.
T2  - Journal of Immunotoxicology
T1  - Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats
IS  - 1
VL  - 10
SP  - 375
EP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1061
ER  - 

@article{
author = "Popov Aleksandrov, Aleksandra and Belij, Sandra and Subota, Vesna S and Zolotarevski, Lidija D and Mirkov, Ivana and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2013",
abstract = "Warfarin is a Vitamin K (VK) antagonist that affects Vitamin K-dependent (VKD) processes, including blood coagulation, as well as processes unrelated to hemostasis such as bone growth, calcification, and growth of some cell types. In addition, warfarin exerts influence on some non-VKD-related activities, including anti-tumor and immunomodulating activity. With respect to the latter, both immune stimulating and suppressive effects have been noted in different experimental systems. To explore the in vivo immunomodulatory potential of warfarin on one type of activity (i.e., cytokine production) in two different immune cell populations (i.e., mononuclear or polymorphonuclear cells), effects of subchronic oral warfarin intake in rats on pro-inflammatory cytokine (i.e., TNF alpha, IL-6) production by peripheral blood mononuclear and polymorphonuclear cells (granulocytes) was examined. Differential effects of warfarin intake on TNF alpha and IL-6 were noted, depending on the type of peripheral blood leukocytes and on the cytokine examined. Specifically, a lack of effect on TNF alpha and a priming of IL-6 production by mononuclear cells along with a decrease in TNF alpha and a lack of effect on IL-6 in polymorphonuclear cells were seen in warfarin-exposed hosts. The cell-and cytokine-dependent effects from subchronic oral warfarin intake on peripheral blood leukocytes demonstrated in this study could, possibly, differentially affect reactions mediated by these cells. Ultimately, the observed effects in rats might have implications for those humans who are on long-term/prolonged warfarin therapy.",
journal = "Journal of Immunotoxicology",
title = "Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats",
number = "1",
volume = "10",
pages = "375-24",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1061"
}

Popov Aleksandrov, A., Belij, S., Subota, V. S., Zolotarevski, L. D., Mirkov, I., Kataranovski, D. S.,& Kataranovski, M.. (2013). Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats. in Journal of Immunotoxicology, 10(1), 375-24.
https://hdl.handle.net/21.15107/rcub_ibiss_1061

Popov Aleksandrov A, Belij S, Subota VS, Zolotarevski LD, Mirkov I, Kataranovski DS, Kataranovski M. Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats. in Journal of Immunotoxicology. 2013;10(1):375-24.
https://hdl.handle.net/21.15107/rcub_ibiss_1061 .

Popov Aleksandrov, Aleksandra, Belij, Sandra, Subota, Vesna S, Zolotarevski, Lidija D, Mirkov, Ivana, Kataranovski, Dragan S., Kataranovski, Milena, "Oral warfarin affects peripheral blood leukocyte IL-6 and TNF alpha production in rats" in Journal of Immunotoxicology, 10, no. 1 (2013):375-24,
https://hdl.handle.net/21.15107/rcub_ibiss_1061 .

TY  - JOUR
AU  - Belij, Sandra
AU  - Miljković, Đorđe
AU  - Popov Aleksandrov, Aleksandra
AU  - Subota, Vesna S
AU  - Timotijević, Gordana S
AU  - Slavić, Marija
AU  - Mirkov, Ivana
AU  - Kataranovski, Dragan S.
AU  - Kataranovski, Milena
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1185
AB  - Warfarin affects mainly vitamin K dependent (VKD) processes, but the effects on some non-VKD-related activities such as tumor growth inhibition and mononuclear cell-mediated immune reactions were shown as well. In this study, the effect of subchronic (30 days) oral warfarin (0.35 mg/l and 3.5 mg/l) intake on peripheral blood granulocytes in rats was investigated. Increase in prothrombin and partial thromboplastin time at high warfarin dose reflected its basic activity. Priming effect for respiratory burst was noted at both warfarin doses, while only high warfarin dose resulted in priming for adhesion, the rise in intracellular myeloperoxidase content/release and stimulation of nitric oxide production. Differential effects of high warfarin dose were noted on granulocyte cytokines IL-6 (lack of the effect), TNF-alpha (decreased release and mRNA expression) and IL-12 (increase in mRNA for IL-12 subunits p35 and p40). Changes in granulocytes seems not to rely on mitogen activated kinases p38 and ERK. Warfarin intake was associated with an increase in circulating IL-6, fibrinogen and haptoglobin and with changes in the activity of erythrocyte antioxidant enzymes superoxide dismutase and catalase. The effects of oral warfarin intake on peripheral blood granulocytes demonstrated in this study might be relevant for oral anticoagulant therapy strategies in humans. (C) 2012 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats
IS  - 5
VL  - 50
EP  - 1507
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1185
ER  - 

@article{
author = "Belij, Sandra and Miljković, Đorđe and Popov Aleksandrov, Aleksandra and Subota, Vesna S and Timotijević, Gordana S and Slavić, Marija and Mirkov, Ivana and Kataranovski, Dragan S. and Kataranovski, Milena",
year = "2012",
abstract = "Warfarin affects mainly vitamin K dependent (VKD) processes, but the effects on some non-VKD-related activities such as tumor growth inhibition and mononuclear cell-mediated immune reactions were shown as well. In this study, the effect of subchronic (30 days) oral warfarin (0.35 mg/l and 3.5 mg/l) intake on peripheral blood granulocytes in rats was investigated. Increase in prothrombin and partial thromboplastin time at high warfarin dose reflected its basic activity. Priming effect for respiratory burst was noted at both warfarin doses, while only high warfarin dose resulted in priming for adhesion, the rise in intracellular myeloperoxidase content/release and stimulation of nitric oxide production. Differential effects of high warfarin dose were noted on granulocyte cytokines IL-6 (lack of the effect), TNF-alpha (decreased release and mRNA expression) and IL-12 (increase in mRNA for IL-12 subunits p35 and p40). Changes in granulocytes seems not to rely on mitogen activated kinases p38 and ERK. Warfarin intake was associated with an increase in circulating IL-6, fibrinogen and haptoglobin and with changes in the activity of erythrocyte antioxidant enzymes superoxide dismutase and catalase. The effects of oral warfarin intake on peripheral blood granulocytes demonstrated in this study might be relevant for oral anticoagulant therapy strategies in humans. (C) 2012 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats",
number = "5",
volume = "50",
pages = "1507",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1185"
}

Belij, S., Miljković, Đ., Popov Aleksandrov, A., Subota, V. S., Timotijević, G. S., Slavić, M., Mirkov, I., Kataranovski, D. S.,& Kataranovski, M.. (2012). Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats. in Food and Chemical Toxicology, 50(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1185

Belij S, Miljković Đ, Popov Aleksandrov A, Subota VS, Timotijević GS, Slavić M, Mirkov I, Kataranovski DS, Kataranovski M. Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats. in Food and Chemical Toxicology. 2012;50(5):null-1507.
https://hdl.handle.net/21.15107/rcub_ibiss_1185 .

Belij, Sandra, Miljković, Đorđe, Popov Aleksandrov, Aleksandra, Subota, Vesna S, Timotijević, Gordana S, Slavić, Marija, Mirkov, Ivana, Kataranovski, Dragan S., Kataranovski, Milena, "Effects of subacute oral warfarin administration on peripheral blood granulocytes in rats" in Food and Chemical Toxicology, 50, no. 5 (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1185 .