Lazić, Gospava

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First detection of Coronavirus in bats in Vojvodina province, Serbia

Lupulović, Diana; Paunović, Milan; Budinski, Ivana; Petrović, Tamaš; Karapandža, Branko; Bajić, Branka; Lazić, Sava; Samojlović, Milena; Lazić, Gospava

(Belgrade: Serbian Veterinary Society, Section for Zoonoses, 2019)

TY  - CONF
AU  - Lupulović, Diana
AU  - Paunović, Milan
AU  - Budinski, Ivana
AU  - Petrović, Tamaš
AU  - Karapandža, Branko
AU  - Bajić, Branka
AU  - Lazić, Sava
AU  - Samojlović, Milena
AU  - Lazić, Gospava
PY  - 2019
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4271
AB  - Different bat species are recognized as a reservoir for several emerging viruses,
including Coronaviruses (CoVs) that were responsible for serious human disease
outbreaks during the 21st century, such as Severe Acute Respiratory Syndrome
Coronavirus (SARS-CoV) and Middle East Respiratory Syndrome Coronavirus
(MERS-CoV).
This study describes molecular analysis of coronaviruses in bats in Vojvodina
Province, in the northern part of Serbia. In total, 64 fecal samples were collected from
animals during 2016 and 2017 from 4 different locations within municipalities of Novi
Sad, Kovin and Vršac (one location in Novi Sad and Kovin and two locations in
Vršac). Among 14 analysed bat species, CoV was identified in 3 species (Myotis
nattereri, Rhinolophus ferrumequinum and Miniopterus schreibersii). CoV was
detected by RT-PCR in 29.68% (19/64) tested fecal samples. The highest number of
virus positive samples was identified in the municipality of Kovin (13/23, 56.52%),
followed by one location in Vršac (4/13, 30.76%) and Novi Sad (2/10, 20%), while
no positive bat feces was detected in the second location in Vršac. All tested animals
were apparently healthy and in good condition.
The obtained results, as to our knowledge, represent the first detection of coronavirus
in bats in Vojvodina Province and give a contribution to better understanding of
biodiversity and ecology of these mammals. Although is known that bats can serve as
a potential source of emerging viral pathogens, for the bat species that has been
identified in Serbia so far are not known to be associated to any occurrence of human
diseases in Serbia. Further studies should be conducted to improve the knowledge
about phylogenetic characterization of coronaviruses, as well as other viruses in bat
population in Serbia.
AB  - Различите врсте слепих мишева препознате су као резервоар неколико нових емергентних вируса, укључујући коронавирусе (CoVs) који су одговорни за настанак озбиљних епидемија код људи током 21. века, као што су Тешки акутни респираторни коронавирус синдром (SARS-CoV) и Респираторни коронавирус синдром Средњег истока (MERS-CoV).
Ово истраживање описује молекуларну детекцију коронавируса код слепих мишева у Војводини, у северном делу Србије. Током 2016. и 2017. године прикупљено је 64 узорка фецеса животиња са 4 различита локалитета на подручју општина Нови Сад, Ковин и Вршац (једна локација у Новом Саду и Ковину и две локације у Вршцу). Међу 14 анализираних врста слепих мишева, CoV је идентификован код 3 врсте (Myotis nattereri, Rhinolophus ferrumequinum и Miniopterus schreibersii). Коронавирус је детектован RT-PCR методом у 29,68% (19/64) тестираних узорака фецеса. Највећи број позитивних узорака идентификован је у општини Ковин (13/23, 56,52%), а затим на једној локацији у Вршцу (4/13, 30,76%) и у Новом Саду (2/10, 20%), док на другој локацији у Вршцу није детектован ниједан позитиван узорак фецеса. Све тестиране животиње биле су очигледно здраве и у добром стању.
Добијени резултати представљају прву нама познату детекцију коронавируса код слепих мишева у Војводини и дају допринос бољем разумевању биодиверзитета и екологије ових сисара. Иако је познато да слепи мишеви могу да служе као потенцијални извор вирусних патогена, ниједна врста слепих мишева која је до сада идентификована у Србији није до сада доведена у везу са настанком болести код људи у Србији. Даља истраживања би требала да унапреде сазнање о филогенетској карактеризацији коронавируса, као и других вируса код популације слепих мишева у Србији.
PB  - Belgrade: Serbian Veterinary Society, Section for Zoonoses
C3  - XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani); 2019 Apr 8-10; Novi Sad, Serbia
T1  - First detection of Coronavirus in bats in Vojvodina province, Serbia
T1  - Прва детекција коронавируса код слепих мишева у Војводини, Србија
SP  - 78
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4271
ER  - 
@conference{
author = "Lupulović, Diana and Paunović, Milan and Budinski, Ivana and Petrović, Tamaš and Karapandža, Branko and Bajić, Branka and Lazić, Sava and Samojlović, Milena and Lazić, Gospava",
year = "2019",
abstract = "Different bat species are recognized as a reservoir for several emerging viruses,
including Coronaviruses (CoVs) that were responsible for serious human disease
outbreaks during the 21st century, such as Severe Acute Respiratory Syndrome
Coronavirus (SARS-CoV) and Middle East Respiratory Syndrome Coronavirus
(MERS-CoV).
This study describes molecular analysis of coronaviruses in bats in Vojvodina
Province, in the northern part of Serbia. In total, 64 fecal samples were collected from
animals during 2016 and 2017 from 4 different locations within municipalities of Novi
Sad, Kovin and Vršac (one location in Novi Sad and Kovin and two locations in
Vršac). Among 14 analysed bat species, CoV was identified in 3 species (Myotis
nattereri, Rhinolophus ferrumequinum and Miniopterus schreibersii). CoV was
detected by RT-PCR in 29.68% (19/64) tested fecal samples. The highest number of
virus positive samples was identified in the municipality of Kovin (13/23, 56.52%),
followed by one location in Vršac (4/13, 30.76%) and Novi Sad (2/10, 20%), while
no positive bat feces was detected in the second location in Vršac. All tested animals
were apparently healthy and in good condition.
The obtained results, as to our knowledge, represent the first detection of coronavirus
in bats in Vojvodina Province and give a contribution to better understanding of
biodiversity and ecology of these mammals. Although is known that bats can serve as
a potential source of emerging viral pathogens, for the bat species that has been
identified in Serbia so far are not known to be associated to any occurrence of human
diseases in Serbia. Further studies should be conducted to improve the knowledge
about phylogenetic characterization of coronaviruses, as well as other viruses in bat
population in Serbia., Различите врсте слепих мишева препознате су као резервоар неколико нових емергентних вируса, укључујући коронавирусе (CoVs) који су одговорни за настанак озбиљних епидемија код људи током 21. века, као што су Тешки акутни респираторни коронавирус синдром (SARS-CoV) и Респираторни коронавирус синдром Средњег истока (MERS-CoV).
Ово истраживање описује молекуларну детекцију коронавируса код слепих мишева у Војводини, у северном делу Србије. Током 2016. и 2017. године прикупљено је 64 узорка фецеса животиња са 4 различита локалитета на подручју општина Нови Сад, Ковин и Вршац (једна локација у Новом Саду и Ковину и две локације у Вршцу). Међу 14 анализираних врста слепих мишева, CoV је идентификован код 3 врсте (Myotis nattereri, Rhinolophus ferrumequinum и Miniopterus schreibersii). Коронавирус је детектован RT-PCR методом у 29,68% (19/64) тестираних узорака фецеса. Највећи број позитивних узорака идентификован је у општини Ковин (13/23, 56,52%), а затим на једној локацији у Вршцу (4/13, 30,76%) и у Новом Саду (2/10, 20%), док на другој локацији у Вршцу није детектован ниједан позитиван узорак фецеса. Све тестиране животиње биле су очигледно здраве и у добром стању.
Добијени резултати представљају прву нама познату детекцију коронавируса код слепих мишева у Војводини и дају допринос бољем разумевању биодиверзитета и екологије ових сисара. Иако је познато да слепи мишеви могу да служе као потенцијални извор вирусних патогена, ниједна врста слепих мишева која је до сада идентификована у Србији није до сада доведена у везу са настанком болести код људи у Србији. Даља истраживања би требала да унапреде сазнање о филогенетској карактеризацији коронавируса, као и других вируса код популације слепих мишева у Србији.",
publisher = "Belgrade: Serbian Veterinary Society, Section for Zoonoses",
journal = "XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani); 2019 Apr 8-10; Novi Sad, Serbia",
title = "First detection of Coronavirus in bats in Vojvodina province, Serbia, Прва детекција коронавируса код слепих мишева у Војводини, Србија",
pages = "78-79",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4271"
}
Lupulović, D., Paunović, M., Budinski, I., Petrović, T., Karapandža, B., Bajić, B., Lazić, S., Samojlović, M.,& Lazić, G.. (2019). First detection of Coronavirus in bats in Vojvodina province, Serbia. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani); 2019 Apr 8-10; Novi Sad, Serbia
Belgrade: Serbian Veterinary Society, Section for Zoonoses., 78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_4271
Lupulović D, Paunović M, Budinski I, Petrović T, Karapandža B, Bajić B, Lazić S, Samojlović M, Lazić G. First detection of Coronavirus in bats in Vojvodina province, Serbia. in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani); 2019 Apr 8-10; Novi Sad, Serbia. 2019;:78-79.
https://hdl.handle.net/21.15107/rcub_ibiss_4271 .
Lupulović, Diana, Paunović, Milan, Budinski, Ivana, Petrović, Tamaš, Karapandža, Branko, Bajić, Branka, Lazić, Sava, Samojlović, Milena, Lazić, Gospava, "First detection of Coronavirus in bats in Vojvodina province, Serbia" in XXI Simpozijum epizootiologa i epidemiologa (XXI Epizootiološki dani); 2019 Apr 8-10; Novi Sad, Serbia (2019):78-79,
https://hdl.handle.net/21.15107/rcub_ibiss_4271 .

Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?

Tokodi, Nada; Drobac, Damjana; Meriluoto, Jussi; Lujić, Jelena; Marinović, Zoran; Važić, Tamara; Nybom, Sonja; Simeunović, Jelica; Dulić, Tamara; Lazić, Gospava; Petrović, Tamaš; Vuković-Gačić, Branka; Sunjog, Karolina; Kolarević, Stoimir; Kračun-Kolarević, Margareta; Subakov-Simić, Gordana; Miljanović, Branko; Codd, Geoffrey A.; Svirčev, Zorica

(Elsevier B.V., 2018)

TY  - JOUR
AU  - Tokodi, Nada
AU  - Drobac, Damjana
AU  - Meriluoto, Jussi
AU  - Lujić, Jelena
AU  - Marinović, Zoran
AU  - Važić, Tamara
AU  - Nybom, Sonja
AU  - Simeunović, Jelica
AU  - Dulić, Tamara
AU  - Lazić, Gospava
AU  - Petrović, Tamaš
AU  - Vuković-Gačić, Branka
AU  - Sunjog, Karolina
AU  - Kolarević, Stoimir
AU  - Kračun-Kolarević, Margareta
AU  - Subakov-Simić, Gordana
AU  - Miljanović, Branko
AU  - Codd, Geoffrey A.
AU  - Svirčev, Zorica
PY  - 2018
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0048969718313512
UR  - http://www.ncbi.nlm.nih.gov/pubmed/29710560
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3046
AB  - Cyanobacteria are present in many aquatic ecosystems in Serbia. Lake Ludoš, a wetland area of international significance and an important habitat for waterbirds, has become the subject of intense research interest because of practically continuous blooming of cyanobacteria. Analyses of water samples indicated a deterioration of ecological condition and water quality, and the presence of toxin-producing cyanobacteria (the most abundant Limnothrix redekei, Pseudanabaena limnetica, Planktothrix agardhii and Microcystis spp.). Furthermore, microcystins were detected in plants and animals from the lake: in macrophyte rhizomes (Phragmites communis, Typha latifolia and Nymphaea elegans), and in the muscle, intestines, kidneys, gonads and gills of fish (Carassius gibelio). Moreover, histopathological deleterious effects (liver, kidney, gills and intestines) and DNA damage (liver and gills) were observed in fish. A potential treatment for the reduction of cyanobacterial populations employing hydrogen peroxide was tested during this study. The treatment was not effective in laboratory tests although further in-lake trials are needed to make final conclusions about the applicability of the method. Based on our observations of the cyanobacterial populations and cyanotoxins in the water, as well as other aquatic organisms and, a survey of historical data on Lake Ludoš, it can be concluded that the lake is continuously in a poor ecological state. Conservation of the lake in order to protect the waterbirds (without urgent control of eutrophication) actually endangers them and the rest of the biota in this wetland habitat, and possibly other ecosystems. Thus, urgent measures for restoration are required, so that the preservation of this Ramsar site would be meaningful.
PB  - Elsevier B.V.
T2  - Science of the Total Environment
T1  - Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?
VL  - 635
DO  - 10.1016/j.scitotenv.2018.04.177
SP  - 1047
EP  - 1062
ER  - 
@article{
author = "Tokodi, Nada and Drobac, Damjana and Meriluoto, Jussi and Lujić, Jelena and Marinović, Zoran and Važić, Tamara and Nybom, Sonja and Simeunović, Jelica and Dulić, Tamara and Lazić, Gospava and Petrović, Tamaš and Vuković-Gačić, Branka and Sunjog, Karolina and Kolarević, Stoimir and Kračun-Kolarević, Margareta and Subakov-Simić, Gordana and Miljanović, Branko and Codd, Geoffrey A. and Svirčev, Zorica",
year = "2018",
abstract = "Cyanobacteria are present in many aquatic ecosystems in Serbia. Lake Ludoš, a wetland area of international significance and an important habitat for waterbirds, has become the subject of intense research interest because of practically continuous blooming of cyanobacteria. Analyses of water samples indicated a deterioration of ecological condition and water quality, and the presence of toxin-producing cyanobacteria (the most abundant Limnothrix redekei, Pseudanabaena limnetica, Planktothrix agardhii and Microcystis spp.). Furthermore, microcystins were detected in plants and animals from the lake: in macrophyte rhizomes (Phragmites communis, Typha latifolia and Nymphaea elegans), and in the muscle, intestines, kidneys, gonads and gills of fish (Carassius gibelio). Moreover, histopathological deleterious effects (liver, kidney, gills and intestines) and DNA damage (liver and gills) were observed in fish. A potential treatment for the reduction of cyanobacterial populations employing hydrogen peroxide was tested during this study. The treatment was not effective in laboratory tests although further in-lake trials are needed to make final conclusions about the applicability of the method. Based on our observations of the cyanobacterial populations and cyanotoxins in the water, as well as other aquatic organisms and, a survey of historical data on Lake Ludoš, it can be concluded that the lake is continuously in a poor ecological state. Conservation of the lake in order to protect the waterbirds (without urgent control of eutrophication) actually endangers them and the rest of the biota in this wetland habitat, and possibly other ecosystems. Thus, urgent measures for restoration are required, so that the preservation of this Ramsar site would be meaningful.",
publisher = "Elsevier B.V.",
journal = "Science of the Total Environment",
title = "Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?",
volume = "635",
doi = "10.1016/j.scitotenv.2018.04.177",
pages = "1047-1062"
}
Tokodi, N., Drobac, D., Meriluoto, J., Lujić, J., Marinović, Z., Važić, T., Nybom, S., Simeunović, J., Dulić, T., Lazić, G., Petrović, T., Vuković-Gačić, B., Sunjog, K., Kolarević, S., Kračun-Kolarević, M., Subakov-Simić, G., Miljanović, B., Codd, G. A.,& Svirčev, Z.. (2018). Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?. in Science of the Total Environment
Elsevier B.V.., 635, 1047-1062.
https://doi.org/10.1016/j.scitotenv.2018.04.177
Tokodi N, Drobac D, Meriluoto J, Lujić J, Marinović Z, Važić T, Nybom S, Simeunović J, Dulić T, Lazić G, Petrović T, Vuković-Gačić B, Sunjog K, Kolarević S, Kračun-Kolarević M, Subakov-Simić G, Miljanović B, Codd GA, Svirčev Z. Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?. in Science of the Total Environment. 2018;635:1047-1062.
doi:10.1016/j.scitotenv.2018.04.177 .
Tokodi, Nada, Drobac, Damjana, Meriluoto, Jussi, Lujić, Jelena, Marinović, Zoran, Važić, Tamara, Nybom, Sonja, Simeunović, Jelica, Dulić, Tamara, Lazić, Gospava, Petrović, Tamaš, Vuković-Gačić, Branka, Sunjog, Karolina, Kolarević, Stoimir, Kračun-Kolarević, Margareta, Subakov-Simić, Gordana, Miljanović, Branko, Codd, Geoffrey A., Svirčev, Zorica, "Cyanobacterial effects in Lake Ludoš, Serbia - Is preservation of a degraded aquatic ecosystem justified?" in Science of the Total Environment, 635 (2018):1047-1062,
https://doi.org/10.1016/j.scitotenv.2018.04.177 . .
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