TY  - JOUR
AU  - Oalđe Pavlović, Mariana
AU  - Kolarević, Stoimir
AU  - Đorđević, Jelena
AU  - Jovanović Marić, Jovana
AU  - Lunić, Tanja
AU  - Mandić, Marija
AU  - Kračun-Kolarević, Margareta
AU  - Živković, Jelena
AU  - Alimpić Aradski, Ana
AU  - Marin, Petar D.
AU  - Šavikin, Katarina
AU  - Vuković-Gačić, Branka
AU  - Božić Nedeljković, Biljana
AU  - Duletić-Laušević, Sonja
PY  - 2021
UR  - https://www.mdpi.com/2223-7747/10/11/2306
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4654
AB  - This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.
PB  - Basel: MDPI
T2  - Plants
T1  - A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species
IS  - 11
VL  - 10
DO  - 10.3390/plants10112306
SP  - 2306
ER  - 

@article{
author = "Oalđe Pavlović, Mariana and Kolarević, Stoimir and Đorđević, Jelena and Jovanović Marić, Jovana and Lunić, Tanja and Mandić, Marija and Kračun-Kolarević, Margareta and Živković, Jelena and Alimpić Aradski, Ana and Marin, Petar D. and Šavikin, Katarina and Vuković-Gačić, Branka and Božić Nedeljković, Biljana and Duletić-Laušević, Sonja",
year = "2021",
abstract = "This study was designed to evaluate the genoprotective, antigenotoxic, as well as antitumor potential of methanolic, ethanolic, and aqueous extracts of Melissa officinalis, Mentha × piperita, Ocimum basilicum, Rosmarinus officinalis, Salvia officinalis, and Satureja montana (Lamiaceae), in different model systems. The polyphenols in these extracts were quantified both spectrophotometrically and using HPLC-DAD technique, while DPPH assay was used to assess the antioxidant activity. The genoprotective potential was tested on pUC19 Escherichia coli XL1-blue, and the antigenotoxicity on Salmonella typhimurium TA1535/pSK1002 and human lung fibroblasts, while the antitumor activity was assessed on colorectal cancer cells. Rosmarinic acid, quercetin, rutin, and luteolin-7-O-glucoside were among the identified compounds. Methanolic extracts had the best DPPH-scavenging and SOS-inducing activities, while ethanolic extracts exhibited the highest antigenotoxicity. Additionally, all extracts exhibited genoprotective potential on plasmid DNA. The antitumor effect was mediated by modulation of reactive oxygen species (ROS), nitric oxide (NO) production, and exhibition of genotoxic effects on tumor cells, especially with O. basilicum ethanolic extract. Generally, the investigated extracts were able to provide antioxidant protection for the acellular, prokaryotic, and normal human DNA, while also modulating the production of ROS and NO in tumor cells, leading to genotoxicity toward these cells and their decrease in proliferation.",
publisher = "Basel: MDPI",
journal = "Plants",
title = "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species",
number = "11",
volume = "10",
doi = "10.3390/plants10112306",
pages = "2306"
}

Oalđe Pavlović, M., Kolarević, S., Đorđević, J., Jovanović Marić, J., Lunić, T., Mandić, M., Kračun-Kolarević, M., Živković, J., Alimpić Aradski, A., Marin, P. D., Šavikin, K., Vuković-Gačić, B., Božić Nedeljković, B.,& Duletić-Laušević, S.. (2021). A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants
Basel: MDPI., 10(11), 2306.
https://doi.org/10.3390/plants10112306

Oalđe Pavlović M, Kolarević S, Đorđević J, Jovanović Marić J, Lunić T, Mandić M, Kračun-Kolarević M, Živković J, Alimpić Aradski A, Marin PD, Šavikin K, Vuković-Gačić B, Božić Nedeljković B, Duletić-Laušević S. A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species. in Plants. 2021;10(11):2306.
doi:10.3390/plants10112306 .

Oalđe Pavlović, Mariana, Kolarević, Stoimir, Đorđević, Jelena, Jovanović Marić, Jovana, Lunić, Tanja, Mandić, Marija, Kračun-Kolarević, Margareta, Živković, Jelena, Alimpić Aradski, Ana, Marin, Petar D., Šavikin, Katarina, Vuković-Gačić, Branka, Božić Nedeljković, Biljana, Duletić-Laušević, Sonja, "A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species" in Plants, 10, no. 11 (2021):2306,
https://doi.org/10.3390/plants10112306 . .

TY  - JOUR
AU  - Ehmedah, Adil
AU  - Nedeljković, Predrag
AU  - Dacić, Sanja
AU  - Repac, Jelena
AU  - Drašković-Pavlović, Biljana
AU  - Vučević, Dragana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/22/5426
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4034
AB  - Peripheral nerve injury (PNI) triggers a complex multi-cellular response involving the injured neurons, Schwann cells (SCs), and immune cells, often resulting in poor functional recovery. The aim of this study was to investigate the effects of the treatment with vitamin B (B1, B2, B3, B5, B6, and B12) complex on the interaction between macrophages and SCs during the recovery period after PNI. Transection of the motor branch of the femoral nerve followed by reconstruction by termino-terminal anastomosis was used as an experimental model. Isolated nerves from the sham (S), operated (O), and operated groups treated with the B vitamins (OT group) were used for immunofluorescence analysis. The obtained data indicated that PNI modulates interactions between macrophages and SCs in a time-dependent manner. The treatment with B vitamins complex promoted the M1-to M2-macrophage polarization and accelerated the transition from the non-myelin to myelin-forming SCs, an indicative of SCs maturation. The effect of B vitamins complex on both cell types was accompanied with an increase in macrophage/SC interactions, all of which correlated with the regeneration of the injured nerve. Clearly, the capacity of B vitamins to modulate macrophages-SCs interaction may be promising for the treatment of PNI.
PB  - NLM (Medline)
T2  - Molecules
T1  - Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury
IS  - 22
VL  - 25
DO  - 10.3390/molecules25225426
SP  - 5426
ER  - 

@article{
author = "Ehmedah, Adil and Nedeljković, Predrag and Dacić, Sanja and Repac, Jelena and Drašković-Pavlović, Biljana and Vučević, Dragana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2020",
abstract = "Peripheral nerve injury (PNI) triggers a complex multi-cellular response involving the injured neurons, Schwann cells (SCs), and immune cells, often resulting in poor functional recovery. The aim of this study was to investigate the effects of the treatment with vitamin B (B1, B2, B3, B5, B6, and B12) complex on the interaction between macrophages and SCs during the recovery period after PNI. Transection of the motor branch of the femoral nerve followed by reconstruction by termino-terminal anastomosis was used as an experimental model. Isolated nerves from the sham (S), operated (O), and operated groups treated with the B vitamins (OT group) were used for immunofluorescence analysis. The obtained data indicated that PNI modulates interactions between macrophages and SCs in a time-dependent manner. The treatment with B vitamins complex promoted the M1-to M2-macrophage polarization and accelerated the transition from the non-myelin to myelin-forming SCs, an indicative of SCs maturation. The effect of B vitamins complex on both cell types was accompanied with an increase in macrophage/SC interactions, all of which correlated with the regeneration of the injured nerve. Clearly, the capacity of B vitamins to modulate macrophages-SCs interaction may be promising for the treatment of PNI.",
publisher = "NLM (Medline)",
journal = "Molecules",
title = "Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury",
number = "22",
volume = "25",
doi = "10.3390/molecules25225426",
pages = "5426"
}

Ehmedah, A., Nedeljković, P., Dacić, S., Repac, J., Drašković-Pavlović, B., Vučević, D., Peković, S.,& Božić Nedeljković, B.. (2020). Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury. in Molecules
NLM (Medline)., 25(22), 5426.
https://doi.org/10.3390/molecules25225426

Ehmedah A, Nedeljković P, Dacić S, Repac J, Drašković-Pavlović B, Vučević D, Peković S, Božić Nedeljković B. Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury. in Molecules. 2020;25(22):5426.
doi:10.3390/molecules25225426 .

Ehmedah, Adil, Nedeljković, Predrag, Dacić, Sanja, Repac, Jelena, Drašković-Pavlović, Biljana, Vučević, Dragana, Peković, Sanja, Božić Nedeljković, Biljana, "Effect of Vitamin B Complex Treatment on Macrophages to Schwann Cells Association during Neuroinflammation after Peripheral Nerve Injury" in Molecules, 25, no. 22 (2020):5426,
https://doi.org/10.3390/molecules25225426 . .

TY  - CONF
AU  - Lunić, Tanja
AU  - Oalđe, Mariana
AU  - Mandić, Marija
AU  - Sabovljević, Aneta
AU  - Sabovljević, Marko
AU  - Gašić, Uroš
AU  - Duletić-Laušević, Sonja
AU  - Božić, Bojan
AU  - Božić Nedeljković, Biljana
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4106
AB  - Mosses have recently been recognized as promising sources of novel biologically active compounds applicable in the prevention of numerous pathological conditions or as potent adjuvant therapeutics. This study aimed to examine the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory, antineurodegenerative, and antitumor activities) of the moss H. cupressiforme extracts. The corresponding extracts were obtained using solvents of different polarities and their mixtures with Soxhlet extractor and further characterized by spectrophotometric assays and liquid chromatography coupled to mass spectrometry (LC-MS). The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), total reduction power, and β-carotene bleaching assays. Further on, the inhibitory activity on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase was tested. Additionally, biocompatibility, antitumor, and anti-inflammatory potential were tested on MRC-5, HCT-116, MDA-MB-231, and BV2 cells, respectively.

Chemical analysis of the moss extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. The major compounds identified by LC-MS in H. cupressiforme extracts were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. Biochemical assays revealed the significant immunomodulatory potential of examined extracts. Moreover, significant antiproliferative potential against human breast cancer cells – MDA-MB-231, with the proliferation inhibition rate up to 50%, and acceptable biocompatibility was observed. Also, a significant decrease in NO production, observed in lipopolysaccharide-stimulated BV2 cells, implies potential anti-neuroinflammatory application. Overall, obtained results qualify the moss H. cupressiforme as a highly promising candidate for more detailed examination and also putative therapeutical application.
PB  - Basel: MDPI
C3  - 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.
T1  - Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts
DO  - 10.3390/ECMC2020-07282
ER  - 

@conference{
author = "Lunić, Tanja and Oalđe, Mariana and Mandić, Marija and Sabovljević, Aneta and Sabovljević, Marko and Gašić, Uroš and Duletić-Laušević, Sonja and Božić, Bojan and Božić Nedeljković, Biljana",
year = "2020",
abstract = "Mosses have recently been recognized as promising sources of novel biologically active compounds applicable in the prevention of numerous pathological conditions or as potent adjuvant therapeutics. This study aimed to examine the chemical composition and immunomodulatory potential (antioxidant, antidiabetic, anti-neuroinflammatory, antineurodegenerative, and antitumor activities) of the moss H. cupressiforme extracts. The corresponding extracts were obtained using solvents of different polarities and their mixtures with Soxhlet extractor and further characterized by spectrophotometric assays and liquid chromatography coupled to mass spectrometry (LC-MS). The antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH), total reduction power, and β-carotene bleaching assays. Further on, the inhibitory activity on α-glucosidase, α-amylase, acetylcholinesterase, and tyrosinase was tested. Additionally, biocompatibility, antitumor, and anti-inflammatory potential were tested on MRC-5, HCT-116, MDA-MB-231, and BV2 cells, respectively.

Chemical analysis of the moss extracts revealed the presence of flavonoids, phenolic acids, and triterpenoids. The major compounds identified by LC-MS in H. cupressiforme extracts were kaempferol and five phenolic acids: p-hydroxybenzoic, protocatechuic, p-coumaric, gallic, and caffeic acid. Biochemical assays revealed the significant immunomodulatory potential of examined extracts. Moreover, significant antiproliferative potential against human breast cancer cells – MDA-MB-231, with the proliferation inhibition rate up to 50%, and acceptable biocompatibility was observed. Also, a significant decrease in NO production, observed in lipopolysaccharide-stimulated BV2 cells, implies potential anti-neuroinflammatory application. Overall, obtained results qualify the moss H. cupressiforme as a highly promising candidate for more detailed examination and also putative therapeutical application.",
publisher = "Basel: MDPI",
journal = "6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.",
title = "Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts",
doi = "10.3390/ECMC2020-07282"
}

Lunić, T., Oalđe, M., Mandić, M., Sabovljević, A., Sabovljević, M., Gašić, U., Duletić-Laušević, S., Božić, B.,& Božić Nedeljković, B.. (2020). Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.
Basel: MDPI..
https://doi.org/10.3390/ECMC2020-07282

Lunić T, Oalđe M, Mandić M, Sabovljević A, Sabovljević M, Gašić U, Duletić-Laušević S, Božić B, Božić Nedeljković B. Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts. in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online.. 2020;.
doi:10.3390/ECMC2020-07282 .

Lunić, Tanja, Oalđe, Mariana, Mandić, Marija, Sabovljević, Aneta, Sabovljević, Marko, Gašić, Uroš, Duletić-Laušević, Sonja, Božić, Bojan, Božić Nedeljković, Biljana, "Chemical characterization and immunomodulatory potential of the moss HypnumcupressiformeHedw. extracts" in 6th International Electronic Conference on Medicinal Chemistry: session Round Table on Natural Products; 2020 Nov 1-30; Online. (2020),
https://doi.org/10.3390/ECMC2020-07282 . .

TY  - CONF
AU  - Oalđe, Mariana
AU  - Mandić, Marija
AU  - Gašić, Uroš
AU  - Lunić, Tanja
AU  - Kolarević, Stoimir
AU  - Vuković-Gačić, Branka
AU  - Alimpić Aradski, Ana
AU  - Marin, Petar
AU  - Božić Nedeljković, Biljana
AU  - Duletić-Laušević, Sonja
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4104
AB  - The physiological imbalance between the production and accumulation of reactive oxygen species (ROS), known as oxidative stress, is responsible for developing and progression of a plethora of diseases – cancer, among others. Since plants are recognized as potential antioxidant agents, the aim of this study was to chemically characterize ethanolic extracts of 18 species from Lamiaceae family using liquid chromatography-mass spectrometry (LC-MS), followed by testing the antioxidant activity using ABTS assay. Biocompatibility of the investigated extracts was assessed by MTT assay on normal human lung fibroblasts (MRC-5). Finally, antiproliferative activity was evaluated using MTT assay on human colorectal cancer (HCT-116) cell line, while the effects of extracts on superoxide anion radical (O2•-) production were tested using NBT assay. Melissa officinalis, Mentha piperita, Origanum majorana, O. vulgare, and Thymus vulgaris showed the highest antioxidant activity against ABTS radicals. LC-MS analysis indicated that these extracts mostly contain caffeic, protocatechuic and p-coumaric acids. The extracts were biocompatible with MRC-5 cell line. Interestingly, Lavandula angustifolia and Ocimum basilicum extracts, which were less active against ABTS radicals, showed a significant antiproliferative effect against HCT-116. Moreover, L. angustifolia and T. vulgaris extracts significantly stimulated the production of O2•- in HCT-116, indicating potential antitumor activity. The extracts that expressed antiproliferative effect and stimulated ROS production contain higher amounts of rosmarinic, caffeic and chlorogenic acids, naringin, luteolin, apigenin and cirsimaritin, indicating that some of them, or more probably their synergistic effects, are responsible for the extracts’ ability to moderate the production of ROS in HCT-116 cells.
PB  - Basel: MDPI
C3  - 6th International Electronic Conference on Medicinal Chemistry: Online; 2020 Nov 1-30
T1  - Chemical characterization of Lamiaceae plant extracts – the quest for novel immunomodulators
DO  - 10.3390/ECMC2020-07280
ER  - 

@conference{
author = "Oalđe, Mariana and Mandić, Marija and Gašić, Uroš and Lunić, Tanja and Kolarević, Stoimir and Vuković-Gačić, Branka and Alimpić Aradski, Ana and Marin, Petar and Božić Nedeljković, Biljana and Duletić-Laušević, Sonja",
year = "2020",
abstract = "The physiological imbalance between the production and accumulation of reactive oxygen species (ROS), known as oxidative stress, is responsible for developing and progression of a plethora of diseases – cancer, among others. Since plants are recognized as potential antioxidant agents, the aim of this study was to chemically characterize ethanolic extracts of 18 species from Lamiaceae family using liquid chromatography-mass spectrometry (LC-MS), followed by testing the antioxidant activity using ABTS assay. Biocompatibility of the investigated extracts was assessed by MTT assay on normal human lung fibroblasts (MRC-5). Finally, antiproliferative activity was evaluated using MTT assay on human colorectal cancer (HCT-116) cell line, while the effects of extracts on superoxide anion radical (O2•-) production were tested using NBT assay. Melissa officinalis, Mentha piperita, Origanum majorana, O. vulgare, and Thymus vulgaris showed the highest antioxidant activity against ABTS radicals. LC-MS analysis indicated that these extracts mostly contain caffeic, protocatechuic and p-coumaric acids. The extracts were biocompatible with MRC-5 cell line. Interestingly, Lavandula angustifolia and Ocimum basilicum extracts, which were less active against ABTS radicals, showed a significant antiproliferative effect against HCT-116. Moreover, L. angustifolia and T. vulgaris extracts significantly stimulated the production of O2•- in HCT-116, indicating potential antitumor activity. The extracts that expressed antiproliferative effect and stimulated ROS production contain higher amounts of rosmarinic, caffeic and chlorogenic acids, naringin, luteolin, apigenin and cirsimaritin, indicating that some of them, or more probably their synergistic effects, are responsible for the extracts’ ability to moderate the production of ROS in HCT-116 cells.",
publisher = "Basel: MDPI",
journal = "6th International Electronic Conference on Medicinal Chemistry: Online; 2020 Nov 1-30",
title = "Chemical characterization of Lamiaceae plant extracts – the quest for novel immunomodulators",
doi = "10.3390/ECMC2020-07280"
}

Oalđe, M., Mandić, M., Gašić, U., Lunić, T., Kolarević, S., Vuković-Gačić, B., Alimpić Aradski, A., Marin, P., Božić Nedeljković, B.,& Duletić-Laušević, S.. (2020). Chemical characterization of Lamiaceae plant extracts – the quest for novel immunomodulators. in 6th International Electronic Conference on Medicinal Chemistry: Online; 2020 Nov 1-30
Basel: MDPI..
https://doi.org/10.3390/ECMC2020-07280

Oalđe M, Mandić M, Gašić U, Lunić T, Kolarević S, Vuković-Gačić B, Alimpić Aradski A, Marin P, Božić Nedeljković B, Duletić-Laušević S. Chemical characterization of Lamiaceae plant extracts – the quest for novel immunomodulators. in 6th International Electronic Conference on Medicinal Chemistry: Online; 2020 Nov 1-30. 2020;.
doi:10.3390/ECMC2020-07280 .

Oalđe, Mariana, Mandić, Marija, Gašić, Uroš, Lunić, Tanja, Kolarević, Stoimir, Vuković-Gačić, Branka, Alimpić Aradski, Ana, Marin, Petar, Božić Nedeljković, Biljana, Duletić-Laušević, Sonja, "Chemical characterization of Lamiaceae plant extracts – the quest for novel immunomodulators" in 6th International Electronic Conference on Medicinal Chemistry: Online; 2020 Nov 1-30 (2020),
https://doi.org/10.3390/ECMC2020-07280 . .

TY  - JOUR
AU  - Ehmedah, Adil
AU  - Nedeljković, Predrag
AU  - Dacic, Sanja
AU  - Repac, Jelena
AU  - Drašković Pavlović, Biljana
AU  - Vučević, Dragana
AU  - Peković, Sanja
AU  - Božić Nedeljković, Biljana
PY  - 2019
UR  - https://www.mdpi.com/1420-3049/24/24/4615
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3578
AB  - Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans.
PB  - Multidisciplinary Digital Publishing Institute
T2  - Molecules (Basel, Switzerland)
T1  - Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.
IS  - 24
VL  - 24
DO  - 10.3390/molecules24244615
SP  - 4615
ER  - 

@article{
author = "Ehmedah, Adil and Nedeljković, Predrag and Dacic, Sanja and Repac, Jelena and Drašković Pavlović, Biljana and Vučević, Dragana and Peković, Sanja and Božić Nedeljković, Biljana",
year = "2019",
abstract = "Peripheral nerve injury (PNI) leads to a series of cellular and molecular events necessary for axon regeneration and reinnervation of target tissues, among which inflammation is crucial for the orchestration of all these processes. Macrophage activation underlies the pathogenesis of PNI and is characterized by morphological/phenotype transformation from proinflammatory (M1) to an anti-inflammatory (M2) type with different functions in the inflammatory and reparative process. The aim of this study was to evaluate influence of the vitamin B (B1, B2, B3, B5, B6, and B12) complex on the process of neuroinflammation that is in part regulated by l-type CaV1.2 calcium channels. A controlled transection of the motor branch of the femoral peripheral nerve was used as an experimental model. Animals were sacrificed after 1, 3, 7, and 14 injections of vitamin B complex. Isolated nerves were used for immunofluorescence analysis. Treatment with vitamin B complex decreased expression of proinflammatory and increased expression of anti-inflammatory cytokines, thus contributing to the resolution of neuroinflammation. In parallel, B vitamins decreased the number of M1 macrophages that expressed the CaV1.2 channel, and increased the number of M2 macrophages that expressed this channel, suggesting their role in M1/M2 transition after PNI. In conclusion, B vitamins had the potential for treatment of neuroinflammation and neuroregeneration and thereby might be an effective therapy for PNI in humans.",
publisher = "Multidisciplinary Digital Publishing Institute",
journal = "Molecules (Basel, Switzerland)",
title = "Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.",
number = "24",
volume = "24",
doi = "10.3390/molecules24244615",
pages = "4615"
}

Ehmedah, A., Nedeljković, P., Dacic, S., Repac, J., Drašković Pavlović, B., Vučević, D., Peković, S.,& Božić Nedeljković, B.. (2019). Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.. in Molecules (Basel, Switzerland)
Multidisciplinary Digital Publishing Institute., 24(24), 4615.
https://doi.org/10.3390/molecules24244615

Ehmedah A, Nedeljković P, Dacic S, Repac J, Drašković Pavlović B, Vučević D, Peković S, Božić Nedeljković B. Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury.. in Molecules (Basel, Switzerland). 2019;24(24):4615.
doi:10.3390/molecules24244615 .

Ehmedah, Adil, Nedeljković, Predrag, Dacic, Sanja, Repac, Jelena, Drašković Pavlović, Biljana, Vučević, Dragana, Peković, Sanja, Božić Nedeljković, Biljana, "Vitamin B Complex Treatment Attenuates Local Inflammation after Peripheral Nerve Injury." in Molecules (Basel, Switzerland), 24, no. 24 (2019):4615,
https://doi.org/10.3390/molecules24244615 . .