TY  - JOUR
AU  - Đurašević, Siniša
AU  - Pejić, Snežana
AU  - Grigorov, Ilijana
AU  - Nikolić, Gorana
AU  - Mitić-Ćulafić, Dragana
AU  - Dragićević, Milan
AU  - Đorđević, Jelena
AU  - Todorović Vukotić, Nevena
AU  - Đorđević, Neda
AU  - Todorović, Ana
AU  - Drakulić, Dunja
AU  - Veljković, Filip
AU  - Pajović, Snežana B.
AU  - Todorović, Zoran
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4289
AB  - Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.
PB  - MDPI
T2  - Antioxidants
T1  - Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes
IS  - 6
VL  - 10
DO  - 10.3390/antiox10060911
SP  - 911
ER  - 

@article{
author = "Đurašević, Siniša and Pejić, Snežana and Grigorov, Ilijana and Nikolić, Gorana and Mitić-Ćulafić, Dragana and Dragićević, Milan and Đorđević, Jelena and Todorović Vukotić, Nevena and Đorđević, Neda and Todorović, Ana and Drakulić, Dunja and Veljković, Filip and Pajović, Snežana B. and Todorović, Zoran",
year = "2021",
abstract = "Thioacetamide (TAA) is widely used to study liver toxicity accompanied by oxidative stress, inflammation, cell necrosis, fibrosis, cholestasis, and hepatocellular carcinoma. As an efficient free radical’s scavenger, C60 fullerene is considered a potential liver-protective agent in chemically-induced liver injury. In the present work, we examined the hepatoprotective effects of two C60 doses dissolved in virgin olive oil against TAA-induced hepatotoxicity in rats. We showed that TAA-induced increase in liver oxidative stress, judged by the changes in the activities of SOD, CAT, GPx, GR, GST, the content of GSH and 4-HNE, and expression of HO-1, MnSOD, and CuZnSOD, was more effectively ameliorated with a lower C60 dose. Improvement in liver antioxidative status caused by C60 was accompanied by a decrease in liver HMGB1 expression and an increase in nuclear Nrf2/NF-κB p65 ratio, suggesting a reduction in inflammation, necrosis and fibrosis. These results were in accordance with liver histology analysis, liver comet assay, and changes in serum levels of ALT, AST, and AP. The changes observed in gut microbiome support detrimental effects of TAA and hepatoprotective effects of low C60 dose. Less protective effects of a higher C60 dose could be a consequence of its enhanced aggregation and related pro-oxidant role.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes",
number = "6",
volume = "10",
doi = "10.3390/antiox10060911",
pages = "911"
}

Đurašević, S., Pejić, S., Grigorov, I., Nikolić, G., Mitić-Ćulafić, D., Dragićević, M., Đorđević, J., Todorović Vukotić, N., Đorđević, N., Todorović, A., Drakulić, D., Veljković, F., Pajović, S. B.,& Todorović, Z.. (2021). Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants
MDPI., 10(6), 911.
https://doi.org/10.3390/antiox10060911

Đurašević S, Pejić S, Grigorov I, Nikolić G, Mitić-Ćulafić D, Dragićević M, Đorđević J, Todorović Vukotić N, Đorđević N, Todorović A, Drakulić D, Veljković F, Pajović SB, Todorović Z. Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes. in Antioxidants. 2021;10(6):911.
doi:10.3390/antiox10060911 .

Đurašević, Siniša, Pejić, Snežana, Grigorov, Ilijana, Nikolić, Gorana, Mitić-Ćulafić, Dragana, Dragićević, Milan, Đorđević, Jelena, Todorović Vukotić, Nevena, Đorđević, Neda, Todorović, Ana, Drakulić, Dunja, Veljković, Filip, Pajović, Snežana B., Todorović, Zoran, "Effects of C60 Fullerene on Thioacetamide-Induced Rat Liver Toxicity and Gut Microbiome Changes" in Antioxidants, 10, no. 6 (2021):911,
https://doi.org/10.3390/antiox10060911 . .

TY  - JOUR
AU  - Đorđević, Ana
AU  - Veličković, Nataša
AU  - Drakulić, Dunja R.
AU  - Šećerov, Bojana Lj.
AU  - Grković, Ivana S.
AU  - Milošević, Maja S.
AU  - Horvat, Anica I.
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/492
AB  - Ionizing radiation is commonly used in the treatment of brain tumors but it can impair cognitive functions, such as learning and memory. Since cognitive dysfunctions are predominantly result of cell death by apoptosis in hippocampal cells, in this study we analyzed acute effects of cranial gamma-irradiation (10 Gy) on expression of proapoptotic molecules (p53, Bax) and antiapoptotic molecule Bcl-2, as well as caspase-3 activation and cytochrome c redistribution in the hippocampus of young rats. The selected regimen of irradiation resembles the established animal model for childhood prophylactic cranial radiotherapy. Our results demonstrated that p53 mRNA expression was unchanged after irradiation, while induction of p53 protein was rapid. In parallel, Bax mRNA and protein levels were also increased following irradiation, whereas Bcl-2 expression was not changed during the examined post-irradiation period. These changes were accompanied with early hallmarks of apoptosis, such as increased cytochrome c release and stimulated activation of caspase-3. Overall, this study demonstrates that cranial irradiation is associated with the augmented apoptotic pathway in the rat hippocampus, which could be related to the cognitive decline observed in patients after prophylactic cranial radiotherapy, but also opens perspective in finding radioprotectors that can mitigate radiation injury of normal brain tissue.
AB  - Jonizujuće zračenje se često koristi u tretmanu tumora mozga ali ono može da ošteti kognitivne funkcije, kao što su učenje i pamćenje. Pošto je smanjenje kognitivnih funkcija većinom rezultat ćelijske smrti putem apoptoze u hipokampalnim ćelijama u ovoj studiji su analizirani efekti kranijalnog gama-zračenja (10 Gy) na ekspresiju proapoptotskih (p53, Bax) i antiapoptotskih molekula (Bcl-2), kao i na aktivaciju kaspaze-3 i redistribuciju citohroma c u hipokampusu mladih pacova. Odabrani režim zračenja odgovara uspostavljenom životinjskom modelu za dečiju profilaktičku kranijalnu radioterapiju. Naši rezultati pokazuju da je ekspresija p53 i RNK nepromenjena nakon zračenja, dok je indukcija p53 proteina veoma brza. Istovremeno Bax iRNK i protein su povećani nakon zračenja, dok je ekspresija Bcl-2 nepromenjena tokom ispitivanog perioda. Ove promene su praćene ranim znacima apoptoze, kao što su povećano oslobađanje citohroma c i aktivacija kaspaze-3. Generalno, ova studija pokazuje da je kranijalno zračenje povezano sa stimulisanim apoptotskim putem u hipokampusu pacova, što se može povezati sa kognitivnim oštećenjima uočenih kod pacijenata nakon profilaktičke kranijalne radioterapije, ali takođe otvara perspektive u pronalaženju radioprotektora koji smanjuju oštećenje normalnog nervnog tkiva nakon zračenja.
T2  - Nuclear Technology and Radiation Protection
T1  - Delovanje zračenja na indukciju apoptotske ćelijske smrti u hipokampusu pacova
T1  - Radiation-mediated induction of apoptotic cell death in rat hippocampus
IS  - 2
VL  - 28
SP  - 212
EP  - 220
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_492
ER  - 

@article{
author = "Đorđević, Ana and Veličković, Nataša and Drakulić, Dunja R. and Šećerov, Bojana Lj. and Grković, Ivana S. and Milošević, Maja S. and Horvat, Anica I.",
year = "2013, 2013",
abstract = "Ionizing radiation is commonly used in the treatment of brain tumors but it can impair cognitive functions, such as learning and memory. Since cognitive dysfunctions are predominantly result of cell death by apoptosis in hippocampal cells, in this study we analyzed acute effects of cranial gamma-irradiation (10 Gy) on expression of proapoptotic molecules (p53, Bax) and antiapoptotic molecule Bcl-2, as well as caspase-3 activation and cytochrome c redistribution in the hippocampus of young rats. The selected regimen of irradiation resembles the established animal model for childhood prophylactic cranial radiotherapy. Our results demonstrated that p53 mRNA expression was unchanged after irradiation, while induction of p53 protein was rapid. In parallel, Bax mRNA and protein levels were also increased following irradiation, whereas Bcl-2 expression was not changed during the examined post-irradiation period. These changes were accompanied with early hallmarks of apoptosis, such as increased cytochrome c release and stimulated activation of caspase-3. Overall, this study demonstrates that cranial irradiation is associated with the augmented apoptotic pathway in the rat hippocampus, which could be related to the cognitive decline observed in patients after prophylactic cranial radiotherapy, but also opens perspective in finding radioprotectors that can mitigate radiation injury of normal brain tissue., Jonizujuće zračenje se često koristi u tretmanu tumora mozga ali ono može da ošteti kognitivne funkcije, kao što su učenje i pamćenje. Pošto je smanjenje kognitivnih funkcija većinom rezultat ćelijske smrti putem apoptoze u hipokampalnim ćelijama u ovoj studiji su analizirani efekti kranijalnog gama-zračenja (10 Gy) na ekspresiju proapoptotskih (p53, Bax) i antiapoptotskih molekula (Bcl-2), kao i na aktivaciju kaspaze-3 i redistribuciju citohroma c u hipokampusu mladih pacova. Odabrani režim zračenja odgovara uspostavljenom životinjskom modelu za dečiju profilaktičku kranijalnu radioterapiju. Naši rezultati pokazuju da je ekspresija p53 i RNK nepromenjena nakon zračenja, dok je indukcija p53 proteina veoma brza. Istovremeno Bax iRNK i protein su povećani nakon zračenja, dok je ekspresija Bcl-2 nepromenjena tokom ispitivanog perioda. Ove promene su praćene ranim znacima apoptoze, kao što su povećano oslobađanje citohroma c i aktivacija kaspaze-3. Generalno, ova studija pokazuje da je kranijalno zračenje povezano sa stimulisanim apoptotskim putem u hipokampusu pacova, što se može povezati sa kognitivnim oštećenjima uočenih kod pacijenata nakon profilaktičke kranijalne radioterapije, ali takođe otvara perspektive u pronalaženju radioprotektora koji smanjuju oštećenje normalnog nervnog tkiva nakon zračenja.",
journal = "Nuclear Technology and Radiation Protection",
title = "Delovanje zračenja na indukciju apoptotske ćelijske smrti u hipokampusu pacova, Radiation-mediated induction of apoptotic cell death in rat hippocampus",
number = "2",
volume = "28",
pages = "212-220",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_492"
}

Đorđević, A., Veličković, N., Drakulić, D. R., Šećerov, B. Lj., Grković, I. S., Milošević, M. S.,& Horvat, A. I.. (2013). Delovanje zračenja na indukciju apoptotske ćelijske smrti u hipokampusu pacova. in Nuclear Technology and Radiation Protection, 28(2), 212-220.
https://hdl.handle.net/21.15107/rcub_ibiss_492

Đorđević A, Veličković N, Drakulić DR, Šećerov BL, Grković IS, Milošević MS, Horvat AI. Delovanje zračenja na indukciju apoptotske ćelijske smrti u hipokampusu pacova. in Nuclear Technology and Radiation Protection. 2013;28(2):212-220.
https://hdl.handle.net/21.15107/rcub_ibiss_492 .

Đorđević, Ana, Veličković, Nataša, Drakulić, Dunja R., Šećerov, Bojana Lj., Grković, Ivana S., Milošević, Maja S., Horvat, Anica I., "Delovanje zračenja na indukciju apoptotske ćelijske smrti u hipokampusu pacova" in Nuclear Technology and Radiation Protection, 28, no. 2 (2013):212-220,
https://hdl.handle.net/21.15107/rcub_ibiss_492 .