TY  - CONF
AU  - Anđus, Pavle
AU  - Perić, Mina
AU  - Nikolić, Ljiljana
AU  - Bataveljić, Danijela
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5513
AB  - Non-neuronal cells of glial origin play an essential
role in ALS onset and progression. Amer gaining
knowledge on the role of astrocytes in the disease
with particular reference to the inwardly rectifying
potassium channel Kir4.1 we aimed to examine the
functional properties of microglia and oligodendrocytes in the spinal cord of the ALS SOD1G93A rat
focusing on the expression and functional signilcance of Kir4.1.
Microglia in the ALS rat spinal cords showed
remarkable clustering in ventral horns, already in
presymptomatic animals. Colocalization of Kir4.1
and microglial Iba1 staining was 2-3 times more
abundant in presymptomatic as well as in symptomatic animals compared to individual cells. It was
also shown that these clusters bare a higher accumulation and colocalization of Kir4.1 and Iba1 with
mutated SOD1 compared to individual cells.
se spinal cord microglial cells were cultured and
patch-clamped using an innovative movable microscope stage to facilitate the gigaseal formation.
sese measurements demonstrated a decrease of
Ba2+-sensitive Kir currents.
se expression of Kir4.1 was markedly diminished in the dysmorphic ALS oligodendrocytes of
the degenerative phenotype. se cells isolated and
cultured from the SOD1G93A spinal cord showed
no change in processes ramilcation, but expressed
a lower level of Kir4.1. Whole-cell patch-clamp
recordings revealed compromised membrane
biophysical properties and diminished inward currents in ALS oligodendrocytes, with a particularly
decreased Ba2+-sensitive Kir current.
Altogether, our lndings provide the evidence
of a modiled Kir4.1 expression and function in
SOD1G93A glia with this channel’s particular abundance in clusters resembling ALS-specilc plaques.
PB  - Zagreb: Department of Neurology, University Hospital Centre Zagreb
C3  - Abstracts: International Conference on Neurological Disorders and Neurorestoration; 2022 May 19-22; Dubrovnik, Croatia
T1  - Kir4.1 channel- A universal target in ALS glia
SP  - 13
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5513
ER  - 

@conference{
editor = "Mitrečić, Dinko, Petravić, Damir",
author = "Anđus, Pavle and Perić, Mina and Nikolić, Ljiljana and Bataveljić, Danijela",
year = "2022",
abstract = "Non-neuronal cells of glial origin play an essential
role in ALS onset and progression. Amer gaining
knowledge on the role of astrocytes in the disease
with particular reference to the inwardly rectifying
potassium channel Kir4.1 we aimed to examine the
functional properties of microglia and oligodendrocytes in the spinal cord of the ALS SOD1G93A rat
focusing on the expression and functional signilcance of Kir4.1.
Microglia in the ALS rat spinal cords showed
remarkable clustering in ventral horns, already in
presymptomatic animals. Colocalization of Kir4.1
and microglial Iba1 staining was 2-3 times more
abundant in presymptomatic as well as in symptomatic animals compared to individual cells. It was
also shown that these clusters bare a higher accumulation and colocalization of Kir4.1 and Iba1 with
mutated SOD1 compared to individual cells.
se spinal cord microglial cells were cultured and
patch-clamped using an innovative movable microscope stage to facilitate the gigaseal formation.
sese measurements demonstrated a decrease of
Ba2+-sensitive Kir currents.
se expression of Kir4.1 was markedly diminished in the dysmorphic ALS oligodendrocytes of
the degenerative phenotype. se cells isolated and
cultured from the SOD1G93A spinal cord showed
no change in processes ramilcation, but expressed
a lower level of Kir4.1. Whole-cell patch-clamp
recordings revealed compromised membrane
biophysical properties and diminished inward currents in ALS oligodendrocytes, with a particularly
decreased Ba2+-sensitive Kir current.
Altogether, our lndings provide the evidence
of a modiled Kir4.1 expression and function in
SOD1G93A glia with this channel’s particular abundance in clusters resembling ALS-specilc plaques.",
publisher = "Zagreb: Department of Neurology, University Hospital Centre Zagreb",
journal = "Abstracts: International Conference on Neurological Disorders and Neurorestoration; 2022 May 19-22; Dubrovnik, Croatia",
title = "Kir4.1 channel- A universal target in ALS glia",
pages = "13",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5513"
}

Mitrečić, D., Petravić, D., Anđus, P., Perić, M., Nikolić, L.,& Bataveljić, D.. (2022). Kir4.1 channel- A universal target in ALS glia. in Abstracts: International Conference on Neurological Disorders and Neurorestoration; 2022 May 19-22; Dubrovnik, Croatia
Zagreb: Department of Neurology, University Hospital Centre Zagreb., 13.
https://hdl.handle.net/21.15107/rcub_ibiss_5513

Mitrečić D, Petravić D, Anđus P, Perić M, Nikolić L, Bataveljić D. Kir4.1 channel- A universal target in ALS glia. in Abstracts: International Conference on Neurological Disorders and Neurorestoration; 2022 May 19-22; Dubrovnik, Croatia. 2022;:13.
https://hdl.handle.net/21.15107/rcub_ibiss_5513 .

Mitrečić, Dinko, Petravić, Damir, Anđus, Pavle, Perić, Mina, Nikolić, Ljiljana, Bataveljić, Danijela, "Kir4.1 channel- A universal target in ALS glia" in Abstracts: International Conference on Neurological Disorders and Neurorestoration; 2022 May 19-22; Dubrovnik, Croatia (2022):13,
https://hdl.handle.net/21.15107/rcub_ibiss_5513 .