TY  - JOUR
AU  - Nikolić, Biljana
AU  - Kovačević, Dragan
AU  - Mladenović-Drinić, Snežana
AU  - Nikolić, Ana
AU  - Mitić, Zorica
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0534-00121801069N
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3088
AB  - Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo.
T2  - Genetika
T1  - Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites
IS  - 1
VL  - 50
DO  - 10.2298/GENSR1801069N
SP  - 69
EP  - 84
ER  - 

@article{
author = "Nikolić, Biljana and Kovačević, Dragan and Mladenović-Drinić, Snežana and Nikolić, Ana and Mitić, Zorica and Bojović, Srđan and Marin, Petar D.",
year = "2018",
abstract = "Genetic relationships among 12 taxa from subgenera Pinus and Strobus were studied through fourteen microsatellite markers, previously developed for Pinus taeda. To our knowledge, this is the first comparative study of pines using nuclear EST-microsatellites (EST-SSRs). The total number of detected alleles in all investigated taxa was 72 (5.14 in average). The numbers of alleles per locus and PIC values for estimated markers ranged from 3 to 7, and from 0.43 to 0.81, respectively. Presented results are in accordance with majority of previous genetic investigations and infrageneric classification of genus Pinus up to the sectional level, while subsectional position of some species has still not dismissed, especially regarding relict ones. According to nuclear EST-SSRs, Pinus heldreichii is in early-diverging position within subsection Pinaster and shows the greatest closeness with P. halepensis, while Pinus peuce doesn't have basal position within subsection Strobus being more close to P. strobus than to P. wallichiana. Furthermore, the closest connections in subsection Pinus were found between two Pinus nigra subspecies (dalmatica and nigra) as well as between P. sylvestris and P. mugo.",
journal = "Genetika",
title = "Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites",
number = "1",
volume = "50",
doi = "10.2298/GENSR1801069N",
pages = "69-84"
}

Nikolić, B., Kovačević, D., Mladenović-Drinić, S., Nikolić, A., Mitić, Z., Bojović, S.,& Marin, P. D.. (2018). Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites. in Genetika, 50(1), 69-84.
https://doi.org/10.2298/GENSR1801069N

Nikolić B, Kovačević D, Mladenović-Drinić S, Nikolić A, Mitić Z, Bojović S, Marin PD. Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites. in Genetika. 2018;50(1):69-84.
doi:10.2298/GENSR1801069N .

Nikolić, Biljana, Kovačević, Dragan, Mladenović-Drinić, Snežana, Nikolić, Ana, Mitić, Zorica, Bojović, Srđan, Marin, Petar D., "Relationships among some pines from subgenera Pinus and Strobus revealed by nuclear EST-microsatellites" in Genetika, 50, no. 1 (2018):69-84,
https://doi.org/10.2298/GENSR1801069N . .

TY  - JOUR
AU  - Kovačević, Dragan
AU  - Nikolić, Biljana
AU  - Mladenović-Drinić, Snežana
AU  - Bojović, Srđan
AU  - Dodoš, Tanja
AU  - Rajčević, Nemanja
AU  - Marin, Petar D.
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/449
AB  - Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD) markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%). The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana) and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor) than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus.
AB  - Prikazana su proučavanja sa ciljem utvrđivanja genetičke veze 10 različitih vrsta familije Pinaceae putem nasumično umnoženih polimorfnih DNA (RAPD) markera. Osamnaest proizvoljnih RAPD prajmera produkovalo je 123 fragmenata od kojih 107 polimorfnih (87%). Vrednosti koeficijenta sličnosti su varirale od 0.34 do 0.67. Najviši koeficijent sličnosti je detektovan između Pinus wallichiana i P. strobus, kao i između Picea abies i P. orientalis,a najniži između tri vrste roda Pinus (P. heldreichii,P. peuce i P. wallichiana)i Picea omorika. Analiza RAPD markera potvrdila je genetičku vezu između vrsta. Rod Picea se jasno razdvojio od roda Pinus i bliži je rodu Abies (A. concolor) nego rodu Pinus, što potvrđuje dosadašnje brojne uporedno-morfološke, anatomske, hemotaksonomske i molekularne rezultate ovih blisko srodnih rodova. Nadalje, na osnovu naših rezultata, jasno se razdvajaju analizirane vrste borova iz različitih podrodova-Pinus i Strobus, što je, takođe, u saglasnosti sa savremenom intrageneričkom klasifikacijom roda Pinus.
T2  - Genetika
T1  - Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera
T1  - Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers
IS  - 2
VL  - 45
SP  - 493
EP  - 502
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_449
ER  - 

@article{
author = "Kovačević, Dragan and Nikolić, Biljana and Mladenović-Drinić, Snežana and Bojović, Srđan and Dodoš, Tanja and Rajčević, Nemanja and Marin, Petar D.",
year = "2013, 2013",
abstract = "Studies were undertaken to identify genetic relationships among ten different species of the family Pinaceae through randomly amplified polymorphic DNA (RAPD) markers. Eighteen arbitrary RAPD primers produced 123 fragments of which 107 were polymorphic (87%). The similarity coefficient values varied from 0.34 to 0.67. The highest similarity coefficient was detected between Pinus wallichiana and P. strobus as well as between Picea abies and P. orientalis, and the lowest was detected between threePinus species (P. heldreichii, P. peuce and P. wallichiana) and Picea omorika. The analysis of RAPD markers confirmed the genetic relationships among species. GenusPicea is clearly separated from genus Pinus and is closer to genus Abies (A. concolor) than to genus Pinus, what confirms up-to-date numerous comparative-morphological, anatomical, chemotaxonomic and molecular results of these closely related genera. Furthermore, on the basis of our results, pine species from different subgenera -Pinus and Strobus are clearly separated. This statement is in agreement with contemporary intrageneric classification of the genus Pinus., Prikazana su proučavanja sa ciljem utvrđivanja genetičke veze 10 različitih vrsta familije Pinaceae putem nasumično umnoženih polimorfnih DNA (RAPD) markera. Osamnaest proizvoljnih RAPD prajmera produkovalo je 123 fragmenata od kojih 107 polimorfnih (87%). Vrednosti koeficijenta sličnosti su varirale od 0.34 do 0.67. Najviši koeficijent sličnosti je detektovan između Pinus wallichiana i P. strobus, kao i između Picea abies i P. orientalis,a najniži između tri vrste roda Pinus (P. heldreichii,P. peuce i P. wallichiana)i Picea omorika. Analiza RAPD markera potvrdila je genetičku vezu između vrsta. Rod Picea se jasno razdvojio od roda Pinus i bliži je rodu Abies (A. concolor) nego rodu Pinus, što potvrđuje dosadašnje brojne uporedno-morfološke, anatomske, hemotaksonomske i molekularne rezultate ovih blisko srodnih rodova. Nadalje, na osnovu naših rezultata, jasno se razdvajaju analizirane vrste borova iz različitih podrodova-Pinus i Strobus, što je, takođe, u saglasnosti sa savremenom intrageneričkom klasifikacijom roda Pinus.",
journal = "Genetika",
title = "Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera, Genetic relationships among some Pinus, Picea and Abies species revealed by RAPD markers",
number = "2",
volume = "45",
pages = "493-502",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_449"
}

Kovačević, D., Nikolić, B., Mladenović-Drinić, S., Bojović, S., Dodoš, T., Rajčević, N.,& Marin, P. D.. (2013). Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera. in Genetika, 45(2), 493-502.
https://hdl.handle.net/21.15107/rcub_ibiss_449

Kovačević D, Nikolić B, Mladenović-Drinić S, Bojović S, Dodoš T, Rajčević N, Marin PD. Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera. in Genetika. 2013;45(2):493-502.
https://hdl.handle.net/21.15107/rcub_ibiss_449 .

Kovačević, Dragan, Nikolić, Biljana, Mladenović-Drinić, Snežana, Bojović, Srđan, Dodoš, Tanja, Rajčević, Nemanja, Marin, Petar D., "Genetički odnosi između nekih Pinus, Picea i Abies vrsta utvrđeni pomoću RAPD markera" in Genetika, 45, no. 2 (2013):493-502,
https://hdl.handle.net/21.15107/rcub_ibiss_449 .

TY  - JOUR
AU  - Bojović, Srđan
AU  - Heizmann, Philippe
AU  - Dražić, Dragana
AU  - Kovačević, Dragan
AU  - Marin, Petar
AU  - Matić, Rada
AU  - Popović, Zorica
AU  - Jurc, Maja
PY  - 2013
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/447
AB  - PCR-RAPD markers revealed individual variation in F. ornus. A total of 122 fragments were amplified using 7 primers and of these 97 fragments were polymorphic. The percentage of polymorphic loci was between 53.3% and 74.6% with an average of 63.1%. The mean gene diversity for all populations was 0.30 and the mean Shannon's index was 0.44. Of the total genetic variation 87% was intra-population whilst 13% was inter-population. The Mantel test revealed significant correlation between genetic and geographical distance matrice. Results herein represent the first use of molecular genetic (DNA) markers to characterize genetic variation in F. ornus populations. The partition of total genetic variance indicates a relatively restricted population differentiation as expected in outcrossing species. Present and future information on genetic structure and variability in F. ornus needs to be incorporated into strategies for the preservation of genetic resources of tree species.
AB  - U radu su korišćeni PCR-RAPD markeri radi procene individualnih varijacija kod vrste F. ornus. Ukupno 122 fragmenta je amplificirano korišćenjem 7 prajmera, i među njima je bilo 97 polimorfnih fragmenata. Procenat polimorfnih lokusa se kretao između 53.3% i 74.6% sa prosečnom vrednošću od 63.1%. Srednji diverzitet gena za sve ispitivane populacije je iznosio 0.30, dok je srednji Shannon's index imao vrednost 0.44. Od totalne genetičke varijabilnosti 87% pripada intra-populacionoj varijabilnost, a 13% inter-populacionoj. Mantel test je pokazao značajne korelacije između matrica genetičke i geografske distance. Rezultati ovog rada predstavljaju prvu upotrebu molekularno genetičkih (DNA) markera u cilju određivanja genetičke varijabilnosti populacija F. ornus. Odnosi unutar ukupne genetičke varijabilnosti ukazuju na relativno ograničenu populacionu diferencijacije u odnosu na vrednosti koje su očekivane kod stranooplodne vrste. Na osnovu ovih kao i budućih informacija koje se odnose na genetičku strukturu i varijabilnost vrste F. ornus potrebno je kreirati strategije za očuvanje genetičkih resursa drvenastih vrsta.
T2  - Genetika
T1  - Diverzitet vrste Fraxinus ornus iz Srbije i Crne Gore procenjena RAPD metodom
T1  - Diversity of Fraxinus ornus from Serbia and Montenegro as revealed by RAPDs
IS  - 1
VL  - 45
SP  - 51
EP  - 62
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_447
ER  - 

@article{
author = "Bojović, Srđan and Heizmann, Philippe and Dražić, Dragana and Kovačević, Dragan and Marin, Petar and Matić, Rada and Popović, Zorica and Jurc, Maja",
year = "2013, 2013",
abstract = "PCR-RAPD markers revealed individual variation in F. ornus. A total of 122 fragments were amplified using 7 primers and of these 97 fragments were polymorphic. The percentage of polymorphic loci was between 53.3% and 74.6% with an average of 63.1%. The mean gene diversity for all populations was 0.30 and the mean Shannon's index was 0.44. Of the total genetic variation 87% was intra-population whilst 13% was inter-population. The Mantel test revealed significant correlation between genetic and geographical distance matrice. Results herein represent the first use of molecular genetic (DNA) markers to characterize genetic variation in F. ornus populations. The partition of total genetic variance indicates a relatively restricted population differentiation as expected in outcrossing species. Present and future information on genetic structure and variability in F. ornus needs to be incorporated into strategies for the preservation of genetic resources of tree species., U radu su korišćeni PCR-RAPD markeri radi procene individualnih varijacija kod vrste F. ornus. Ukupno 122 fragmenta je amplificirano korišćenjem 7 prajmera, i među njima je bilo 97 polimorfnih fragmenata. Procenat polimorfnih lokusa se kretao između 53.3% i 74.6% sa prosečnom vrednošću od 63.1%. Srednji diverzitet gena za sve ispitivane populacije je iznosio 0.30, dok je srednji Shannon's index imao vrednost 0.44. Od totalne genetičke varijabilnosti 87% pripada intra-populacionoj varijabilnost, a 13% inter-populacionoj. Mantel test je pokazao značajne korelacije između matrica genetičke i geografske distance. Rezultati ovog rada predstavljaju prvu upotrebu molekularno genetičkih (DNA) markera u cilju određivanja genetičke varijabilnosti populacija F. ornus. Odnosi unutar ukupne genetičke varijabilnosti ukazuju na relativno ograničenu populacionu diferencijacije u odnosu na vrednosti koje su očekivane kod stranooplodne vrste. Na osnovu ovih kao i budućih informacija koje se odnose na genetičku strukturu i varijabilnost vrste F. ornus potrebno je kreirati strategije za očuvanje genetičkih resursa drvenastih vrsta.",
journal = "Genetika",
title = "Diverzitet vrste Fraxinus ornus iz Srbije i Crne Gore procenjena RAPD metodom, Diversity of Fraxinus ornus from Serbia and Montenegro as revealed by RAPDs",
number = "1",
volume = "45",
pages = "51-62",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_447"
}

Bojović, S., Heizmann, P., Dražić, D., Kovačević, D., Marin, P., Matić, R., Popović, Z.,& Jurc, M.. (2013). Diverzitet vrste Fraxinus ornus iz Srbije i Crne Gore procenjena RAPD metodom. in Genetika, 45(1), 51-62.
https://hdl.handle.net/21.15107/rcub_ibiss_447

Bojović S, Heizmann P, Dražić D, Kovačević D, Marin P, Matić R, Popović Z, Jurc M. Diverzitet vrste Fraxinus ornus iz Srbije i Crne Gore procenjena RAPD metodom. in Genetika. 2013;45(1):51-62.
https://hdl.handle.net/21.15107/rcub_ibiss_447 .

Bojović, Srđan, Heizmann, Philippe, Dražić, Dragana, Kovačević, Dragan, Marin, Petar, Matić, Rada, Popović, Zorica, Jurc, Maja, "Diverzitet vrste Fraxinus ornus iz Srbije i Crne Gore procenjena RAPD metodom" in Genetika, 45, no. 1 (2013):51-62,
https://hdl.handle.net/21.15107/rcub_ibiss_447 .