TY  - JOUR
AU  - Sarac, Zorica
AU  - Aleksic, Jelena M.
AU  - Dodos, Tanja
AU  - Rajcevic, Nemanja
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2063
AB  - Due to the current lack of nuclear microsatellites (simple sequence
   repeats SSRs) specifically developed for Pinus nigra, an important
   European coniferous species, we cross-species amplified 12 EST-SSRs
   (expressed sequence tagged SSRs) developed for other Pinus species in P.
   nigra in order to delineate loci which can be used for assessing levels
   of genetic diversity and genetic structuring in this species. We
   amplified these loci in individuals from seven populations from the
   central Balkans representing four recognized infraspecific taxa of P.
   nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica).
   Contrary to expectations on high transferability of EST-SSRs into
   related species, only three out of 12 tested loci were successfully
   amplified in P. nigra, but they displayed lack/low levels of
   polymorphism or generated multilocus amplification products. Thus, our
   estimates on levels of genetic diversity (HE = 0.183) and genetic
   differentiation (F-ST = 0.007) were based on variability of a single
   locus harboring four alleles only and they should be taken with
   cautions. Our study highlights the need for the development of
   high-resolution molecular markers, such as co-dominant genic or genomic
   SSRs or predominantly biallelic SNPs, or utilization of anonymous
   dominant markers, such as AFLPs, for genotyping in P. nigra.
T2  - Genetika-Belgrade
T1  - CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra
IS  - 1
VL  - 47
DO  - 10.2298/GENSR1501205S
SP  - 205
EP  - 217
ER  - 

@article{
author = "Sarac, Zorica and Aleksic, Jelena M. and Dodos, Tanja and Rajcevic, Nemanja and Bojović, Srđan and Marin, Petar D.",
year = "2015",
abstract = "Due to the current lack of nuclear microsatellites (simple sequence
   repeats SSRs) specifically developed for Pinus nigra, an important
   European coniferous species, we cross-species amplified 12 EST-SSRs
   (expressed sequence tagged SSRs) developed for other Pinus species in P.
   nigra in order to delineate loci which can be used for assessing levels
   of genetic diversity and genetic structuring in this species. We
   amplified these loci in individuals from seven populations from the
   central Balkans representing four recognized infraspecific taxa of P.
   nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica).
   Contrary to expectations on high transferability of EST-SSRs into
   related species, only three out of 12 tested loci were successfully
   amplified in P. nigra, but they displayed lack/low levels of
   polymorphism or generated multilocus amplification products. Thus, our
   estimates on levels of genetic diversity (HE = 0.183) and genetic
   differentiation (F-ST = 0.007) were based on variability of a single
   locus harboring four alleles only and they should be taken with
   cautions. Our study highlights the need for the development of
   high-resolution molecular markers, such as co-dominant genic or genomic
   SSRs or predominantly biallelic SNPs, or utilization of anonymous
   dominant markers, such as AFLPs, for genotyping in P. nigra.",
journal = "Genetika-Belgrade",
title = "CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra",
number = "1",
volume = "47",
doi = "10.2298/GENSR1501205S",
pages = "205-217"
}

Sarac, Z., Aleksic, J. M., Dodos, T., Rajcevic, N., Bojović, S.,& Marin, P. D.. (2015). CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra. in Genetika-Belgrade, 47(1), 205-217.
https://doi.org/10.2298/GENSR1501205S

Sarac Z, Aleksic JM, Dodos T, Rajcevic N, Bojović S, Marin PD. CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra. in Genetika-Belgrade. 2015;47(1):205-217.
doi:10.2298/GENSR1501205S .

Sarac, Zorica, Aleksic, Jelena M., Dodos, Tanja, Rajcevic, Nemanja, Bojović, Srđan, Marin, Petar D., "CROSS-SPECIES AMPLIFICATION OF NUCLEAR EST-MICROSATELLITES DEVELOPED FOR
 OTHER Pinus SPECIES IN Pinus nigra" in Genetika-Belgrade, 47, no. 1 (2015):205-217,
https://doi.org/10.2298/GENSR1501205S . .

TY  - JOUR
AU  - Šarac, Zorica
AU  - Dodos, Tanja
AU  - Rajčević, Nemanja
AU  - Bojović, Srđan
AU  - Marin, Petar D.
AU  - Aleksić, Jelena M.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2040
AB  - Pinus nigra J.F. Arnold, European black pine, is a typical component of
   Mediterranean and sub-Mediterranean coniferous forests with highly
   fragmentary distribution. Western Mediterranean populations of this
   species have been studied genetically to date, while eastern populations
   from the central Balkans, which are larger and more abundant, are still
   genetically understudied. We analyzed seven populations of P. nigra
   representing all infraspecific taxa recognized within the central
   Balkans (subspecies nigra with varieties nigra and gocensis Dordevic;
   and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and
   banatica (Endl.) Georgescu et Ionescu), with three chloroplast
   microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus.
   Although our molecular data failed to support circumscription of studied
   infraspecific taxa, we found that genetic patterns at both genomes are
   in accordance with those found previously in westward populations of
   this species, that is - exceptionally high levels of genetic diversity
   (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the
   cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and
   genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on
   genealogical relations of mtDNA types currently present in Balkans' and
   Iberian/African populations, we inferred that the ancestral gene pool of
   P. nigra already harbored polymorphism at position 328 prior to the
   divergence to two lineages currently present in westward and eastward
   parts of the species range distribution. Subsequent occurrence of three
   mutations, which distinguish these two lineages, suggests their
   long-term isolation.
T2  - Silva Fennica
T1  - Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data
IS  - 1415
VL  - 49
DO  - 10.14214/sf.1415
ER  - 

@article{
author = "Šarac, Zorica and Dodos, Tanja and Rajčević, Nemanja and Bojović, Srđan and Marin, Petar D. and Aleksić, Jelena M.",
year = "2015",
abstract = "Pinus nigra J.F. Arnold, European black pine, is a typical component of
   Mediterranean and sub-Mediterranean coniferous forests with highly
   fragmentary distribution. Western Mediterranean populations of this
   species have been studied genetically to date, while eastern populations
   from the central Balkans, which are larger and more abundant, are still
   genetically understudied. We analyzed seven populations of P. nigra
   representing all infraspecific taxa recognized within the central
   Balkans (subspecies nigra with varieties nigra and gocensis Dordevic;
   and subspecies pallasiana (Lamb.) Holmboe with varieties pallasiana and
   banatica (Endl.) Georgescu et Ionescu), with three chloroplast
   microsatellites (cpDNA SSRs) and one mitochondrial (mtDNA) locus.
   Although our molecular data failed to support circumscription of studied
   infraspecific taxa, we found that genetic patterns at both genomes are
   in accordance with those found previously in westward populations of
   this species, that is - exceptionally high levels of genetic diversity
   (H-T = 0.949) and low genetic differentiation (G(ST) = 0.024) at the
   cpDNA level, and moderate levels of genetic diversity (H-T = 0.357) and
   genetic differentiation (G(ST) = 0.358) at the mtDNA level. Based on
   genealogical relations of mtDNA types currently present in Balkans' and
   Iberian/African populations, we inferred that the ancestral gene pool of
   P. nigra already harbored polymorphism at position 328 prior to the
   divergence to two lineages currently present in westward and eastward
   parts of the species range distribution. Subsequent occurrence of three
   mutations, which distinguish these two lineages, suggests their
   long-term isolation.",
journal = "Silva Fennica",
title = "Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data",
number = "1415",
volume = "49",
doi = "10.14214/sf.1415"
}

Šarac, Z., Dodos, T., Rajčević, N., Bojović, S., Marin, P. D.,& Aleksić, J. M.. (2015). Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data. in Silva Fennica, 49(1415).
https://doi.org/10.14214/sf.1415

Šarac Z, Dodos T, Rajčević N, Bojović S, Marin PD, Aleksić JM. Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data. in Silva Fennica. 2015;49(1415).
doi:10.14214/sf.1415 .

Šarac, Zorica, Dodos, Tanja, Rajčević, Nemanja, Bojović, Srđan, Marin, Petar D., Aleksić, Jelena M., "Genetic patterns in Pinus nigra from the central Balkans inferred from
 plastid and mitochondrial data" in Silva Fennica, 49, no. 1415 (2015),
https://doi.org/10.14214/sf.1415 . .

TY  - JOUR
AU  - Sarac, Zorica
AU  - Matejić, Jelena S
AU  - Stojanović-Radić, Zorica Z
AU  - Veselinović, Jovana B
AU  - Džamić, Ana M
AU  - Bojović, Srđan
AU  - Marin, Petar D
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/784
AB  - In the current work, in vitro antioxidant, antibacterial, and antifungal activites of the needle terpenes of three taxa of Pinus nigra from Serbia (ssp. nigra, ssp. pallasiana, and var. banatica) were analyzed. The black pine essential oils showed generally weak antioxidative properties tested by two methods (DPPH and ABTS scavenging assays), where the highest activity was identified in P. nigra var. banatica (IC50=25.08 mg/mL and VitC=0.67 mg (vitamin C)/g when tested with the DPPH and ABTS reagents, respectively). In the antimicrobial assays, one fungal (Aspergilus niger) and two bacterial strains (Staphylococcus aureus and Bacillus cereus) showed sensitivity against essential oils of all three P. nigra taxa. The tested oils have been shown to possess inhibitory action in the range from 20.00 to 0.62 mg/mL, where var. banatica exhibited the highest and ssp. nigra the lowest antimicrobial action. In order to determine potential compounds that are responsible for alternative mode of action, molecular docking simulations inside FtsZ (a prokaryotic homolog of tubulin) were performed. Tested compounds were the most abundant terpenoid (germacrene D-4-ol) and its structurally similar terpene (germacrene D), both present in all three essential oils. It was determined that the oxygenated form of the molecule creates stable bonds with investigated enzyme FtsZ, and that this compound, through this mechanism of action participates in the antimicrobial activity. (C) 2014 Elsevier Ltd. All rights reserved.
T2  - Computers in Biology and Medicine
T1  - Biological activity of Pinus nigra terpenes-Evaluation of FtsZ inhibition by selected compounds as contribution to their antimicrobial activity
IS  - null
VL  - 54
SP  - 265
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_784
ER  - 

@article{
author = "Sarac, Zorica and Matejić, Jelena S and Stojanović-Radić, Zorica Z and Veselinović, Jovana B and Džamić, Ana M and Bojović, Srđan and Marin, Petar D",
year = "2014",
abstract = "In the current work, in vitro antioxidant, antibacterial, and antifungal activites of the needle terpenes of three taxa of Pinus nigra from Serbia (ssp. nigra, ssp. pallasiana, and var. banatica) were analyzed. The black pine essential oils showed generally weak antioxidative properties tested by two methods (DPPH and ABTS scavenging assays), where the highest activity was identified in P. nigra var. banatica (IC50=25.08 mg/mL and VitC=0.67 mg (vitamin C)/g when tested with the DPPH and ABTS reagents, respectively). In the antimicrobial assays, one fungal (Aspergilus niger) and two bacterial strains (Staphylococcus aureus and Bacillus cereus) showed sensitivity against essential oils of all three P. nigra taxa. The tested oils have been shown to possess inhibitory action in the range from 20.00 to 0.62 mg/mL, where var. banatica exhibited the highest and ssp. nigra the lowest antimicrobial action. In order to determine potential compounds that are responsible for alternative mode of action, molecular docking simulations inside FtsZ (a prokaryotic homolog of tubulin) were performed. Tested compounds were the most abundant terpenoid (germacrene D-4-ol) and its structurally similar terpene (germacrene D), both present in all three essential oils. It was determined that the oxygenated form of the molecule creates stable bonds with investigated enzyme FtsZ, and that this compound, through this mechanism of action participates in the antimicrobial activity. (C) 2014 Elsevier Ltd. All rights reserved.",
journal = "Computers in Biology and Medicine",
title = "Biological activity of Pinus nigra terpenes-Evaluation of FtsZ inhibition by selected compounds as contribution to their antimicrobial activity",
number = "null",
volume = "54",
pages = "265-78",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_784"
}

Sarac, Z., Matejić, J. S., Stojanović-Radić, Z. Z., Veselinović, J. B., Džamić, A. M., Bojović, S.,& Marin, P. D.. (2014). Biological activity of Pinus nigra terpenes-Evaluation of FtsZ inhibition by selected compounds as contribution to their antimicrobial activity. in Computers in Biology and Medicine, 54(null), 265-78.
https://hdl.handle.net/21.15107/rcub_ibiss_784

Sarac Z, Matejić JS, Stojanović-Radić ZZ, Veselinović JB, Džamić AM, Bojović S, Marin PD. Biological activity of Pinus nigra terpenes-Evaluation of FtsZ inhibition by selected compounds as contribution to their antimicrobial activity. in Computers in Biology and Medicine. 2014;54(null):265-78.
https://hdl.handle.net/21.15107/rcub_ibiss_784 .

Sarac, Zorica, Matejić, Jelena S, Stojanović-Radić, Zorica Z, Veselinović, Jovana B, Džamić, Ana M, Bojović, Srđan, Marin, Petar D, "Biological activity of Pinus nigra terpenes-Evaluation of FtsZ inhibition by selected compounds as contribution to their antimicrobial activity" in Computers in Biology and Medicine, 54, no. null (2014):265-78,
https://hdl.handle.net/21.15107/rcub_ibiss_784 .

TY  - JOUR
AU  - Sarac, Zorica
AU  - Matejic, Jelena S.
AU  - Stojanovic-Radic, Zorica Z.
AU  - Veselinovic, Jovana B.
AU  - Džamić, Ana M.
AU  - Bojović, Srđan
AU  - Marin, Petar D.
PY  - 2014
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2127
AB  - In the current work, in vitro antioxidant, antibacterial, and antifungal
   activites of the needle terpenes of three taxa of Pinus nigra from
   Serbia (ssp. nigra, ssp. pallasiana, and var. banatica) were analyzed.
   The black pine essential oils showed generally weak antioxidative
   properties tested by two methods (DPPH and ABTS scavenging assays),
   where the highest activity was identified in P. nigra var. banatica
   (IC50=25.08 mg/mL and VitC=0.67 mg (vitamin C)/g when tested with the
   DPPH and ABTS reagents, respectively). In the antimicrobial assays, one
   fungal (Aspergilus niger) and two bacterial strains (Staphylococcus
   aureus and Bacillus cereus) showed sensitivity against essential oils of
   all three P. nigra taxa. The tested oils have been shown to possess
   inhibitory action in the range from 20.00 to 0.62 mg/mL, where var.
   banatica exhibited the highest and ssp. nigra the lowest antimicrobial
   action. In order to determine potential compounds that are responsible
   for alternative mode of action, molecular docking simulations inside
   FtsZ (a prokaryotic homolog of tubulin) were performed. Tested compounds
   were the most abundant terpenoid (germacrene D-4-ol) and its
   structurally similar terpene (germacrene D), both present in all three
   essential oils. It was determined that the oxygenated form of the
   molecule creates stable bonds with investigated enzyme FtsZ, and that
   this compound, through this mechanism of action participates in the
   antimicrobial activity. (C) 2014 Elsevier Ltd. All rights reserved.
T2  - Computers in Biology and Medicine
T1  - Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity
VL  - 54
DO  - 10.1016/j.compbiomed.2014.08.022
SP  - 72
EP  - 78
ER  - 

@article{
author = "Sarac, Zorica and Matejic, Jelena S. and Stojanovic-Radic, Zorica Z. and Veselinovic, Jovana B. and Džamić, Ana M. and Bojović, Srđan and Marin, Petar D.",
year = "2014",
abstract = "In the current work, in vitro antioxidant, antibacterial, and antifungal
   activites of the needle terpenes of three taxa of Pinus nigra from
   Serbia (ssp. nigra, ssp. pallasiana, and var. banatica) were analyzed.
   The black pine essential oils showed generally weak antioxidative
   properties tested by two methods (DPPH and ABTS scavenging assays),
   where the highest activity was identified in P. nigra var. banatica
   (IC50=25.08 mg/mL and VitC=0.67 mg (vitamin C)/g when tested with the
   DPPH and ABTS reagents, respectively). In the antimicrobial assays, one
   fungal (Aspergilus niger) and two bacterial strains (Staphylococcus
   aureus and Bacillus cereus) showed sensitivity against essential oils of
   all three P. nigra taxa. The tested oils have been shown to possess
   inhibitory action in the range from 20.00 to 0.62 mg/mL, where var.
   banatica exhibited the highest and ssp. nigra the lowest antimicrobial
   action. In order to determine potential compounds that are responsible
   for alternative mode of action, molecular docking simulations inside
   FtsZ (a prokaryotic homolog of tubulin) were performed. Tested compounds
   were the most abundant terpenoid (germacrene D-4-ol) and its
   structurally similar terpene (germacrene D), both present in all three
   essential oils. It was determined that the oxygenated form of the
   molecule creates stable bonds with investigated enzyme FtsZ, and that
   this compound, through this mechanism of action participates in the
   antimicrobial activity. (C) 2014 Elsevier Ltd. All rights reserved.",
journal = "Computers in Biology and Medicine",
title = "Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity",
volume = "54",
doi = "10.1016/j.compbiomed.2014.08.022",
pages = "72-78"
}

Sarac, Z., Matejic, J. S., Stojanovic-Radic, Z. Z., Veselinovic, J. B., Džamić, A. M., Bojović, S.,& Marin, P. D.. (2014). Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity. in Computers in Biology and Medicine, 54, 72-78.
https://doi.org/10.1016/j.compbiomed.2014.08.022

Sarac Z, Matejic JS, Stojanovic-Radic ZZ, Veselinovic JB, Džamić AM, Bojović S, Marin PD. Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity. in Computers in Biology and Medicine. 2014;54:72-78.
doi:10.1016/j.compbiomed.2014.08.022 .

Sarac, Zorica, Matejic, Jelena S., Stojanovic-Radic, Zorica Z., Veselinovic, Jovana B., Džamić, Ana M., Bojović, Srđan, Marin, Petar D., "Biological activity of Pinus nigra terpenes-Evaluation of FtsZ
 inhibition by selected compounds as contribution to their antimicrobial
 activity" in Computers in Biology and Medicine, 54 (2014):72-78,
https://doi.org/10.1016/j.compbiomed.2014.08.022 . .

TY  - JOUR
AU  - Sarac, Zorica
AU  - Bojović, Srđan
AU  - Nikolić, Biljana M
AU  - Tesević, Vele V
AU  - Đorđević, Iris Z
AU  - Marin, Petar D
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/980
AB  - The essential-oil variability in seven native populations belonging to different infraspecific taxa of Pinus nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica) growing wild in Serbia was analyzed. In the needles of 195 trees from seven populations, 58 essential-oil components were identified. The major components were -pinene (43.6%) and germacrene D (29.8%), comprising together 73.4% of the total oil composition. Based on the average chemical profile of the main terpene components (with contents >5%), the studied populations were found to be the most similar to populations from central Italy and Greece (ssp. nigra). Cluster analysis showed the division of the populations into three principal groups: the first group consisted of PopulationsI, II, III, IV, and V (considered as ssp. nigra group), the second of PopulationVI (ssp. pallasiana group), and the third of PopulationVII, which had the most distinct oil composition (ssp. banatica group). The taxonomic implications of the essential-oil profiles of the investigated taxa of this very complex species are discussed.
T2  - Chemistry & Biodiversity
T1  - Chemotaxonomic Significance of the Terpene Composition in Natural Populations of Pinus nigra JFArnold from Serbia
IS  - 8
VL  - 10
SP  - 731
EP  - 1520
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_980
ER  - 

@article{
author = "Sarac, Zorica and Bojović, Srđan and Nikolić, Biljana M and Tesević, Vele V and Đorđević, Iris Z and Marin, Petar D",
year = "2013",
abstract = "The essential-oil variability in seven native populations belonging to different infraspecific taxa of Pinus nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica) growing wild in Serbia was analyzed. In the needles of 195 trees from seven populations, 58 essential-oil components were identified. The major components were -pinene (43.6%) and germacrene D (29.8%), comprising together 73.4% of the total oil composition. Based on the average chemical profile of the main terpene components (with contents >5%), the studied populations were found to be the most similar to populations from central Italy and Greece (ssp. nigra). Cluster analysis showed the division of the populations into three principal groups: the first group consisted of PopulationsI, II, III, IV, and V (considered as ssp. nigra group), the second of PopulationVI (ssp. pallasiana group), and the third of PopulationVII, which had the most distinct oil composition (ssp. banatica group). The taxonomic implications of the essential-oil profiles of the investigated taxa of this very complex species are discussed.",
journal = "Chemistry & Biodiversity",
title = "Chemotaxonomic Significance of the Terpene Composition in Natural Populations of Pinus nigra JFArnold from Serbia",
number = "8",
volume = "10",
pages = "731-1520",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_980"
}

Sarac, Z., Bojović, S., Nikolić, B. M., Tesević, V. V., Đorđević, I. Z.,& Marin, P. D.. (2013). Chemotaxonomic Significance of the Terpene Composition in Natural Populations of Pinus nigra JFArnold from Serbia. in Chemistry & Biodiversity, 10(8), 731-1520.
https://hdl.handle.net/21.15107/rcub_ibiss_980

Sarac Z, Bojović S, Nikolić BM, Tesević VV, Đorđević IZ, Marin PD. Chemotaxonomic Significance of the Terpene Composition in Natural Populations of Pinus nigra JFArnold from Serbia. in Chemistry & Biodiversity. 2013;10(8):731-1520.
https://hdl.handle.net/21.15107/rcub_ibiss_980 .

Sarac, Zorica, Bojović, Srđan, Nikolić, Biljana M, Tesević, Vele V, Đorđević, Iris Z, Marin, Petar D, "Chemotaxonomic Significance of the Terpene Composition in Natural Populations of Pinus nigra JFArnold from Serbia" in Chemistry & Biodiversity, 10, no. 8 (2013):731-1520,
https://hdl.handle.net/21.15107/rcub_ibiss_980 .

TY  - JOUR
AU  - Bojović, Srđan
AU  - Sarac, Zorica
AU  - Nikolić, Biljana M
AU  - Tesević, Vele V
AU  - Todosijević, Marina
AU  - Veljić, Milan M
AU  - Marin, Petar D
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1075
AB  - This is the first report on the composition and variability of the needle-wax n-alkanes in natural populations of Pinus nigra in Serbia. Samples of 195 trees from seven populations belonging to several infraspecific taxa (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica) were analyzed. In general, the size of the n-alkanes ranged from C16 to C33, with the exception of ssp. nigra, for which it ranged from C18 to C33. The most abundant were C23-, C25-, C27-, and C29-alkanes. The needle waxes of Populations IIII and V were characterized by a higher content of C23-, C25-, and C27-alkanes and a lower content of C24-, C26-, C28-, and C30-alkanes, compared to the other populations, and the trees of these populations could be assigned to ssp. nigra. The samples of Population VI were characterized by higher amounts of C22-, C24-, C30-, and C32-alkanes and lower amounts of C25- and C27-alkanes, and the trees could be considered as ssp. pallasiana. The samples of Population VII, consisting of trees belonging to var. banatica, were richer in C29-, C31-, and C33-alkanes. The wax compositions of Populations IV and V, both composed of trees previously determined as P. nigra var. gocensis, showed a tendency of splitting. Indeed, the alkane composition of Population IV was closer to that of ssp. pallasiana pines, while that of Population V was more similar to that of ssp. nigra pines. From the results presented here, it is obvious that in the central part of the Balkan Peninsula, significant diversification and differentiation of the populations of black pine exists, and these populations could be defined as different intraspecific taxa. Our results also indicate the validity of n-alkanes as chemotaxonomic characters within this aggregate.
T2  - Chemistry & Biodiversity
T1  - Composition of n-Alkanes in Natural Populations of Pinus nigra from Serbia - Chemotaxonomic Implications
IS  - 12
VL  - 9
SP  - 109
EP  - 2774
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1075
ER  - 

@article{
author = "Bojović, Srđan and Sarac, Zorica and Nikolić, Biljana M and Tesević, Vele V and Todosijević, Marina and Veljić, Milan M and Marin, Petar D",
year = "2012",
abstract = "This is the first report on the composition and variability of the needle-wax n-alkanes in natural populations of Pinus nigra in Serbia. Samples of 195 trees from seven populations belonging to several infraspecific taxa (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica) were analyzed. In general, the size of the n-alkanes ranged from C16 to C33, with the exception of ssp. nigra, for which it ranged from C18 to C33. The most abundant were C23-, C25-, C27-, and C29-alkanes. The needle waxes of Populations IIII and V were characterized by a higher content of C23-, C25-, and C27-alkanes and a lower content of C24-, C26-, C28-, and C30-alkanes, compared to the other populations, and the trees of these populations could be assigned to ssp. nigra. The samples of Population VI were characterized by higher amounts of C22-, C24-, C30-, and C32-alkanes and lower amounts of C25- and C27-alkanes, and the trees could be considered as ssp. pallasiana. The samples of Population VII, consisting of trees belonging to var. banatica, were richer in C29-, C31-, and C33-alkanes. The wax compositions of Populations IV and V, both composed of trees previously determined as P. nigra var. gocensis, showed a tendency of splitting. Indeed, the alkane composition of Population IV was closer to that of ssp. pallasiana pines, while that of Population V was more similar to that of ssp. nigra pines. From the results presented here, it is obvious that in the central part of the Balkan Peninsula, significant diversification and differentiation of the populations of black pine exists, and these populations could be defined as different intraspecific taxa. Our results also indicate the validity of n-alkanes as chemotaxonomic characters within this aggregate.",
journal = "Chemistry & Biodiversity",
title = "Composition of n-Alkanes in Natural Populations of Pinus nigra from Serbia - Chemotaxonomic Implications",
number = "12",
volume = "9",
pages = "109-2774",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1075"
}

Bojović, S., Sarac, Z., Nikolić, B. M., Tesević, V. V., Todosijević, M., Veljić, M. M.,& Marin, P. D.. (2012). Composition of n-Alkanes in Natural Populations of Pinus nigra from Serbia - Chemotaxonomic Implications. in Chemistry & Biodiversity, 9(12), 109-2774.
https://hdl.handle.net/21.15107/rcub_ibiss_1075

Bojović S, Sarac Z, Nikolić BM, Tesević VV, Todosijević M, Veljić MM, Marin PD. Composition of n-Alkanes in Natural Populations of Pinus nigra from Serbia - Chemotaxonomic Implications. in Chemistry & Biodiversity. 2012;9(12):109-2774.
https://hdl.handle.net/21.15107/rcub_ibiss_1075 .

Bojović, Srđan, Sarac, Zorica, Nikolić, Biljana M, Tesević, Vele V, Todosijević, Marina, Veljić, Milan M, Marin, Petar D, "Composition of n-Alkanes in Natural Populations of Pinus nigra from Serbia - Chemotaxonomic Implications" in Chemistry & Biodiversity, 9, no. 12 (2012):109-2774,
https://hdl.handle.net/21.15107/rcub_ibiss_1075 .