TY  - JOUR
AU  - Garcia-Oliveira, Paula
AU  - Carreira-Casais, Anxo
AU  - Pereira, Eliana
AU  - Dias, Maria Inês
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Simal-Gandara, Jesus
AU  - Prieto, Miguel A
AU  - Caleja, Cristina
AU  - Barros, Lillian
PY  - 2022
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9571014
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5170
AB  - Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.
PB  - Basel: MDPI
T2  - Molecules
T1  - From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants
IS  - 19
VL  - 27
DO  - 10.3390/molecules27196495
ER  - 

@article{
author = "Garcia-Oliveira, Paula and Carreira-Casais, Anxo and Pereira, Eliana and Dias, Maria Inês and Pereira, Carla and Calhelha, Ricardo C and Stojković, Dejan and Soković, Marina and Simal-Gandara, Jesus and Prieto, Miguel A and Caleja, Cristina and Barros, Lillian",
year = "2022",
abstract = "Several scientific studies have been proving the bioactive effects of many aromatic and medicinal plants associated with the presence of a high number of bioactive compounds, namely phenolic compounds. The antioxidant, anti-inflammatory, and antimicrobial capacities of these molecules have aroused high interest in some industrial sectors, including food, pharmaceuticals, and cosmetics. This work aimed to determine the phenolic profiles of the infusions and hydroethanolic extracts of five plants (Carpobrotus edulis, Genista tridentata, Verbascum sinuatum, Cytisus multiflorus, and Calluna vulgaris) that have been employed in many traditional preparations. In addition, the antioxidant, antimicrobial, anti-inflammatory, and anti-tumoral activity of each different preparation was evaluated using in vitro assays. The HPLC-DAD-ESI/MS profile revealed the presence of eighty phenolic compounds, belonging to seven different families of compounds. Regarding antioxidant properties, the hydroethanolic extract of C. edulis showed a potent effect in the TBARS assay (IC50 = 1.20 µg/mL), while G. tridentata hydroethanolic extract achieved better results in the OxHLIA test (IC50 = 76 µg/mL). For cytotoxic and anti-inflammatory results, V. sinuatum infusions stood out significantly, with GI50 = 59.1-92.1 µg/mL and IC50 = 121.1 µg/mL, respectively. Finally, C. edulis hydroethanolic extract displayed the most relevant antibacterial activity, showing MBC values of 0.25-1 mg/mL, while G. tridentata hydroethanolic extract exerted the greatest antifungal effects (MFC of 0.5-1 mg/mL). The results of this study deepen the knowledge of the phenolic profiles and also provide evidence on the bioactive properties of the species selected, which could be considered highly valuable options for research and application in several sectors, namely food, cosmetics, and pharmaceuticals.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants",
number = "19",
volume = "27",
doi = "10.3390/molecules27196495"
}

Garcia-Oliveira, P., Carreira-Casais, A., Pereira, E., Dias, M. I., Pereira, C., Calhelha, R. C., Stojković, D., Soković, M., Simal-Gandara, J., Prieto, M. A., Caleja, C.,& Barros, L.. (2022). From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules
Basel: MDPI., 27(19).
https://doi.org/10.3390/molecules27196495

Garcia-Oliveira P, Carreira-Casais A, Pereira E, Dias MI, Pereira C, Calhelha RC, Stojković D, Soković M, Simal-Gandara J, Prieto MA, Caleja C, Barros L. From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants. in Molecules. 2022;27(19).
doi:10.3390/molecules27196495 .

Garcia-Oliveira, Paula, Carreira-Casais, Anxo, Pereira, Eliana, Dias, Maria Inês, Pereira, Carla, Calhelha, Ricardo C, Stojković, Dejan, Soković, Marina, Simal-Gandara, Jesus, Prieto, Miguel A, Caleja, Cristina, Barros, Lillian, "From Tradition to Health: Chemical and Bioactive Characterization of Five Traditional Plants" in Molecules, 27, no. 19 (2022),
https://doi.org/10.3390/molecules27196495 . .

TY  - JOUR
AU  - Mascoloti Spréa, Rafael
AU  - Caleja, Cristina
AU  - Pinela, José
AU  - Tiane C., Finimundya
AU  - Calhelha, Ricardo C.
AU  - Kostić, Marina
AU  - Soković, Marina
AU  - Prieto, Miguel A.
AU  - Pereira, Eliana
AU  - Amarala, Joana S.
AU  - Barros, Lillian
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5147
AB  - Medicinal and aromatic plants (MAP) have been described as a source of phenolic compounds with potential as antioxidant, antiproliferative and antimicrobial agents. MAP from the Lamiaceae family (Origanum vulgare L., Thymus vulgaris L., Ocimum basilicum L., Salvia officinalis L., Melissa officinalis L., and Matricaria chamomilla L.) were selected to perform a phytochemical and biological screening for their further exploitation as natural bioactive ingredients. The total content of phenolic compounds varied from 184.02 mg/g extract in M. officinalis to 17.97 mg/g extract in M. chamomilla. Caffeic and rosmarinic acids were the main phenolic acids found in the respective hydroalcoholic extracts. The extracts showed a promising antioxidant activity in vitro, being related the phenolic compositions of the extracts, furthermore, all extracts being able to combat lipid peroxidation in TBARS assays with an IC50 under 26 μg/mL, moreover all the plant extract has prevented the oxidative haemolysis in OxHLIA assays at concentrations below 67 μg/mL in a Δt 60 min and under 118 μg/mL for a Δt 120 min. Regarding to the bactericidal and fungicidal action the plant extracts were able to inhibit growth against bacteria associated with food hazards, such as Salmonella typhimurium (MIC < 1) and Listeria monocytogenes (MIC < 1), regarding to fungicidal activity it can be highlighted the MIC values under to 0.25 for Aspergillus versicolor and Trichoderma viride. Overall, the selected Lamiaceae plants stood out as a source of active phytochemicals that can be used by different industries, such as food and cosmetics.
PB  - Amsterdam : Elsevier
T2  - Food Research International
T1  - Comparative study on the phenolic composition and in vitro bioactivity of medicinal and aromatic plants from the Lamiaceae family
VL  - 161
DO  - 10.1016/j.foodres.2022.111875
SP  - 111875
ER  - 

@article{
author = "Mascoloti Spréa, Rafael and Caleja, Cristina and Pinela, José and Tiane C., Finimundya and Calhelha, Ricardo C. and Kostić, Marina and Soković, Marina and Prieto, Miguel A. and Pereira, Eliana and Amarala, Joana S. and Barros, Lillian",
year = "2022",
abstract = "Medicinal and aromatic plants (MAP) have been described as a source of phenolic compounds with potential as antioxidant, antiproliferative and antimicrobial agents. MAP from the Lamiaceae family (Origanum vulgare L., Thymus vulgaris L., Ocimum basilicum L., Salvia officinalis L., Melissa officinalis L., and Matricaria chamomilla L.) were selected to perform a phytochemical and biological screening for their further exploitation as natural bioactive ingredients. The total content of phenolic compounds varied from 184.02 mg/g extract in M. officinalis to 17.97 mg/g extract in M. chamomilla. Caffeic and rosmarinic acids were the main phenolic acids found in the respective hydroalcoholic extracts. The extracts showed a promising antioxidant activity in vitro, being related the phenolic compositions of the extracts, furthermore, all extracts being able to combat lipid peroxidation in TBARS assays with an IC50 under 26 μg/mL, moreover all the plant extract has prevented the oxidative haemolysis in OxHLIA assays at concentrations below 67 μg/mL in a Δt 60 min and under 118 μg/mL for a Δt 120 min. Regarding to the bactericidal and fungicidal action the plant extracts were able to inhibit growth against bacteria associated with food hazards, such as Salmonella typhimurium (MIC < 1) and Listeria monocytogenes (MIC < 1), regarding to fungicidal activity it can be highlighted the MIC values under to 0.25 for Aspergillus versicolor and Trichoderma viride. Overall, the selected Lamiaceae plants stood out as a source of active phytochemicals that can be used by different industries, such as food and cosmetics.",
publisher = "Amsterdam : Elsevier",
journal = "Food Research International",
title = "Comparative study on the phenolic composition and in vitro bioactivity of medicinal and aromatic plants from the Lamiaceae family",
volume = "161",
doi = "10.1016/j.foodres.2022.111875",
pages = "111875"
}

Mascoloti Spréa, R., Caleja, C., Pinela, J., Tiane C., F., Calhelha, R. C., Kostić, M., Soković, M., Prieto, M. A., Pereira, E., Amarala, J. S.,& Barros, L.. (2022). Comparative study on the phenolic composition and in vitro bioactivity of medicinal and aromatic plants from the Lamiaceae family. in Food Research International
Amsterdam : Elsevier., 161, 111875.
https://doi.org/10.1016/j.foodres.2022.111875

Mascoloti Spréa R, Caleja C, Pinela J, Tiane C. F, Calhelha RC, Kostić M, Soković M, Prieto MA, Pereira E, Amarala JS, Barros L. Comparative study on the phenolic composition and in vitro bioactivity of medicinal and aromatic plants from the Lamiaceae family. in Food Research International. 2022;161:111875.
doi:10.1016/j.foodres.2022.111875 .

Mascoloti Spréa, Rafael, Caleja, Cristina, Pinela, José, Tiane C., Finimundya, Calhelha, Ricardo C., Kostić, Marina, Soković, Marina, Prieto, Miguel A., Pereira, Eliana, Amarala, Joana S., Barros, Lillian, "Comparative study on the phenolic composition and in vitro bioactivity of medicinal and aromatic plants from the Lamiaceae family" in Food Research International, 161 (2022):111875,
https://doi.org/10.1016/j.foodres.2022.111875 . .

TY  - JOUR
AU  - Alaya, Ikbel ben
AU  - Pereira, Eliana
AU  - Dias, Inês Dias
AU  - Pinela, José
AU  - Calhelha, Ricardo C.
AU  - Soković, Marina
AU  - Kostić, Marina
AU  - Prieto, Miguel A.
AU  - Essid, Faten
AU  - Caleja, Cristina
AU  - Ferreira, Isabel C.F.R.
AU  - Barros, Lillian
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4311
AB  - This study was carried out with the aim of optimizing the ultrasound-assisted extraction (UAE) of phenolic compounds from male chestnut flowers (C. sativa Mill) to develop a bioactive extract with potential to be used as a natural antioxidant preservative ingredient in the food industry. Time (t, 1–39 min), solvent concentration (S, 0–100%), and ultrasonic power (P, 5–500 W) were used as the independent variables for a 5-level experimental circumscribed central composite design (CCCD) coupled with response surface methodology (RSM) to optimize the extraction of phenolic compounds by UAE. Regarding the variables, the three showed a significant effect on the extraction of phenolic compounds. The content of phenolic compounds (including flavonoids and tannins) and the extraction yield (extract weight gravimetrically assessed) were the response criteria for the optimization. Based on the statistically validated predictive polynomial models, it was possible to reach a maximum content of phenolic compounds at the global optimal conditions of 24 ± 3 min, 259 ± 16 W, and 51 ± 7% ethanol. Additionally, pentagalloyl-glucoside and trigalloyl-hexahydroxydiphenoyl-glucoside were the major phenolic compounds identified. The optimized extract was then analyzed for their biological properties. The bioactive potential of the chestnut flower extract obtained under these optimized conditions was evaluated using in vitro assays for antioxidant, anti-inflammatory, and antimicrobial activity, as well as cytotoxicity and hepatotoxicity tests. The results revealed that the enriched extract has antioxidant, antitumoral, and anti-inflammatory activities without toxicity issues. Overall, this study allowed to define the optimal conditions for the extraction of phenolic compounds from chestnuts male flowers by UAE, to obtain an enriched extract with biological properties that could be further used as a natural antioxidant ingredient with applications on functional foods
PB  - Basel: MDPI
T2  - Chemosensors
T1  - Development of a Natural Preservative from Chestnut Flowers: Ultrasound-Assisted Extraction Optimization and Functionality Assessment
IS  - 6
VL  - 9
DO  - 10.3390/chemosensors9060141
SP  - 141
ER  - 

@article{
author = "Alaya, Ikbel ben and Pereira, Eliana and Dias, Inês Dias and Pinela, José and Calhelha, Ricardo C. and Soković, Marina and Kostić, Marina and Prieto, Miguel A. and Essid, Faten and Caleja, Cristina and Ferreira, Isabel C.F.R. and Barros, Lillian",
year = "2021",
abstract = "This study was carried out with the aim of optimizing the ultrasound-assisted extraction (UAE) of phenolic compounds from male chestnut flowers (C. sativa Mill) to develop a bioactive extract with potential to be used as a natural antioxidant preservative ingredient in the food industry. Time (t, 1–39 min), solvent concentration (S, 0–100%), and ultrasonic power (P, 5–500 W) were used as the independent variables for a 5-level experimental circumscribed central composite design (CCCD) coupled with response surface methodology (RSM) to optimize the extraction of phenolic compounds by UAE. Regarding the variables, the three showed a significant effect on the extraction of phenolic compounds. The content of phenolic compounds (including flavonoids and tannins) and the extraction yield (extract weight gravimetrically assessed) were the response criteria for the optimization. Based on the statistically validated predictive polynomial models, it was possible to reach a maximum content of phenolic compounds at the global optimal conditions of 24 ± 3 min, 259 ± 16 W, and 51 ± 7% ethanol. Additionally, pentagalloyl-glucoside and trigalloyl-hexahydroxydiphenoyl-glucoside were the major phenolic compounds identified. The optimized extract was then analyzed for their biological properties. The bioactive potential of the chestnut flower extract obtained under these optimized conditions was evaluated using in vitro assays for antioxidant, anti-inflammatory, and antimicrobial activity, as well as cytotoxicity and hepatotoxicity tests. The results revealed that the enriched extract has antioxidant, antitumoral, and anti-inflammatory activities without toxicity issues. Overall, this study allowed to define the optimal conditions for the extraction of phenolic compounds from chestnuts male flowers by UAE, to obtain an enriched extract with biological properties that could be further used as a natural antioxidant ingredient with applications on functional foods",
publisher = "Basel: MDPI",
journal = "Chemosensors",
title = "Development of a Natural Preservative from Chestnut Flowers: Ultrasound-Assisted Extraction Optimization and Functionality Assessment",
number = "6",
volume = "9",
doi = "10.3390/chemosensors9060141",
pages = "141"
}

Alaya, I. b., Pereira, E., Dias, I. D., Pinela, J., Calhelha, R. C., Soković, M., Kostić, M., Prieto, M. A., Essid, F., Caleja, C., Ferreira, I. C.F.R.,& Barros, L.. (2021). Development of a Natural Preservative from Chestnut Flowers: Ultrasound-Assisted Extraction Optimization and Functionality Assessment. in Chemosensors
Basel: MDPI., 9(6), 141.
https://doi.org/10.3390/chemosensors9060141

Alaya IB, Pereira E, Dias ID, Pinela J, Calhelha RC, Soković M, Kostić M, Prieto MA, Essid F, Caleja C, Ferreira IC, Barros L. Development of a Natural Preservative from Chestnut Flowers: Ultrasound-Assisted Extraction Optimization and Functionality Assessment. in Chemosensors. 2021;9(6):141.
doi:10.3390/chemosensors9060141 .

Alaya, Ikbel ben, Pereira, Eliana, Dias, Inês Dias, Pinela, José, Calhelha, Ricardo C., Soković, Marina, Kostić, Marina, Prieto, Miguel A., Essid, Faten, Caleja, Cristina, Ferreira, Isabel C.F.R., Barros, Lillian, "Development of a Natural Preservative from Chestnut Flowers: Ultrasound-Assisted Extraction Optimization and Functionality Assessment" in Chemosensors, 9, no. 6 (2021):141,
https://doi.org/10.3390/chemosensors9060141 . .

TY  - JOUR
AU  - Carpena, Marija
AU  - Caleja, Cristina
AU  - Pereira, Eliana
AU  - Pereira, Carla
AU  - Ćirić, Ana
AU  - Soković, Marina
AU  - Soria-Lopez, Anton
AU  - Fraga-Corral, Maria
AU  - Simal-Gandara, Jesus
AU  - Ferreira, Isabel C. F. R.
AU  - Barros, Lillian
AU  - Prieto, Miguel A.
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4359
AB  - The present work aimed to determine the nutritional composition (ash, protein, fat, car bohydrate content and energy value), phenolic compounds, pigments and organic acids content
of three typical red algae from the Northwest of Spain: Chondrus crispus, Mastocarpus stellatus, and
Gigartina pistillata; as well as their antioxidant and antimicrobial activities. Furthermore, the present
work compared two extraction techniques: conventional heat assisted extraction (HAE) and high
pressure assisted extraction (HPAE) to maximize the yield and the concentration of target compounds.
Different independent variables were considered for the response study. Time (t) and percentage
of ethanol of the solvent (S) were chosen for both techniques and temperature (T) and pressure (P)
were used for HAE and HPAE, respectively. The experiments were designed following a response
surface methodology (RSM) approach. The obtained results showed a similar nutritional composition
between algae samples: low-fat content and high content of proteins, carbohydrates and energy. All
tested algae showed good antioxidant and antimicrobial properties. Finally, HEA demonstrated to
be the most efficient extraction technique. This study confirms the potential of red algae to be part
of the human diet as a source of non-animal protein, due to its nutritional content, phenolic profile,
pigments concentration and bioactive properties, which proves that HAE is the optimum technique
for the extraction maximization.
PB  - Basel: MDPI
T2  - Chemosensors
T1  - Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction
IS  - 6
VL  - 9
DO  - 10.3390/chemosensors9060132
SP  - 132
ER  - 

@article{
author = "Carpena, Marija and Caleja, Cristina and Pereira, Eliana and Pereira, Carla and Ćirić, Ana and Soković, Marina and Soria-Lopez, Anton and Fraga-Corral, Maria and Simal-Gandara, Jesus and Ferreira, Isabel C. F. R. and Barros, Lillian and Prieto, Miguel A.",
year = "2021",
abstract = "The present work aimed to determine the nutritional composition (ash, protein, fat, car bohydrate content and energy value), phenolic compounds, pigments and organic acids content
of three typical red algae from the Northwest of Spain: Chondrus crispus, Mastocarpus stellatus, and
Gigartina pistillata; as well as their antioxidant and antimicrobial activities. Furthermore, the present
work compared two extraction techniques: conventional heat assisted extraction (HAE) and high
pressure assisted extraction (HPAE) to maximize the yield and the concentration of target compounds.
Different independent variables were considered for the response study. Time (t) and percentage
of ethanol of the solvent (S) were chosen for both techniques and temperature (T) and pressure (P)
were used for HAE and HPAE, respectively. The experiments were designed following a response
surface methodology (RSM) approach. The obtained results showed a similar nutritional composition
between algae samples: low-fat content and high content of proteins, carbohydrates and energy. All
tested algae showed good antioxidant and antimicrobial properties. Finally, HEA demonstrated to
be the most efficient extraction technique. This study confirms the potential of red algae to be part
of the human diet as a source of non-animal protein, due to its nutritional content, phenolic profile,
pigments concentration and bioactive properties, which proves that HAE is the optimum technique
for the extraction maximization.",
publisher = "Basel: MDPI",
journal = "Chemosensors",
title = "Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction",
number = "6",
volume = "9",
doi = "10.3390/chemosensors9060132",
pages = "132"
}

Carpena, M., Caleja, C., Pereira, E., Pereira, C., Ćirić, A., Soković, M., Soria-Lopez, A., Fraga-Corral, M., Simal-Gandara, J., Ferreira, I. C. F. R., Barros, L.,& Prieto, M. A.. (2021). Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction. in Chemosensors
Basel: MDPI., 9(6), 132.
https://doi.org/10.3390/chemosensors9060132

Carpena M, Caleja C, Pereira E, Pereira C, Ćirić A, Soković M, Soria-Lopez A, Fraga-Corral M, Simal-Gandara J, Ferreira ICFR, Barros L, Prieto MA. Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction. in Chemosensors. 2021;9(6):132.
doi:10.3390/chemosensors9060132 .

Carpena, Marija, Caleja, Cristina, Pereira, Eliana, Pereira, Carla, Ćirić, Ana, Soković, Marina, Soria-Lopez, Anton, Fraga-Corral, Maria, Simal-Gandara, Jesus, Ferreira, Isabel C. F. R., Barros, Lillian, Prieto, Miguel A., "Red Seaweeds as a Source of Nutrients and Bioactive Compounds: Optimization of the Extraction" in Chemosensors, 9, no. 6 (2021):132,
https://doi.org/10.3390/chemosensors9060132 . .

TY  - JOUR
AU  - Ghada, Bejaoui
AU  - Pereira, Eliana
AU  - Pinela, José
AU  - Prieto, Miguel A.
AU  - Pereira, Carla
AU  - Calhelha, Ricardo C.
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Zaghdoudi, Khalil
AU  - Barros, Lillian
AU  - Ferreira, Isabel C. F. R.
PY  - 2020
UR  - https://www.mdpi.com/1420-3049/25/14/3203
UR  - http://www.ncbi.nlm.nih.gov/pubmed/32674320
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3830
AB  - The potential of passion fruit (Passiflora edulis Sims) epicarp to produce anthocyanin-based colorants with bioactive properties was evaluated. First, a five-level three-factor factorial design coupled with response surface methodology was implemented to optimize the extraction of anthocyanins from dark purple epicarps. The extraction yield and cyanidin-3-O-glucoside content were used as response criteria. The constructed models were fitted to the experimental data and used to calculate the optimal processing conditions (t = 38 min, T = 20 °C, S = 0% ethanol/water (v/v) acidified with citric acid to pH 3, and RS/L = 50 g/L) that lead to maximum responses (3.4 mg/g dried epicarp and 9 mg/g extract). Then, the antioxidant, antimicrobial, and cytotoxic activities of anthocyanin extracts obtained using the optimized method and a conventional extraction method were evaluated in vitro. The extract obtained by the optimized method revealed a higher bioactivity, in agreement with the higher cyanidin-3-O-glucoside content. This study highlighted the coloring and bioactive potential of a bio-based ingredient recycled from a bio-waste, which promotes a sustainable bioeconomy in the agri-food sector.
PB  - NLM (Medline)
T2  - Molecules (Basel, Switzerland)
T1  - Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.
IS  - 14
VL  - 25
DO  - 10.3390/molecules25143203
SP  - 3203
ER  - 

@article{
author = "Ghada, Bejaoui and Pereira, Eliana and Pinela, José and Prieto, Miguel A. and Pereira, Carla and Calhelha, Ricardo C. and Stojković, Dejan and Soković, Marina and Zaghdoudi, Khalil and Barros, Lillian and Ferreira, Isabel C. F. R.",
year = "2020",
abstract = "The potential of passion fruit (Passiflora edulis Sims) epicarp to produce anthocyanin-based colorants with bioactive properties was evaluated. First, a five-level three-factor factorial design coupled with response surface methodology was implemented to optimize the extraction of anthocyanins from dark purple epicarps. The extraction yield and cyanidin-3-O-glucoside content were used as response criteria. The constructed models were fitted to the experimental data and used to calculate the optimal processing conditions (t = 38 min, T = 20 °C, S = 0% ethanol/water (v/v) acidified with citric acid to pH 3, and RS/L = 50 g/L) that lead to maximum responses (3.4 mg/g dried epicarp and 9 mg/g extract). Then, the antioxidant, antimicrobial, and cytotoxic activities of anthocyanin extracts obtained using the optimized method and a conventional extraction method were evaluated in vitro. The extract obtained by the optimized method revealed a higher bioactivity, in agreement with the higher cyanidin-3-O-glucoside content. This study highlighted the coloring and bioactive potential of a bio-based ingredient recycled from a bio-waste, which promotes a sustainable bioeconomy in the agri-food sector.",
publisher = "NLM (Medline)",
journal = "Molecules (Basel, Switzerland)",
title = "Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.",
number = "14",
volume = "25",
doi = "10.3390/molecules25143203",
pages = "3203"
}

Ghada, B., Pereira, E., Pinela, J., Prieto, M. A., Pereira, C., Calhelha, R. C., Stojković, D., Soković, M., Zaghdoudi, K., Barros, L.,& Ferreira, I. C. F. R.. (2020). Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.. in Molecules (Basel, Switzerland)
NLM (Medline)., 25(14), 3203.
https://doi.org/10.3390/molecules25143203

Ghada B, Pereira E, Pinela J, Prieto MA, Pereira C, Calhelha RC, Stojković D, Soković M, Zaghdoudi K, Barros L, Ferreira ICFR. Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties.. in Molecules (Basel, Switzerland). 2020;25(14):3203.
doi:10.3390/molecules25143203 .

Ghada, Bejaoui, Pereira, Eliana, Pinela, José, Prieto, Miguel A., Pereira, Carla, Calhelha, Ricardo C., Stojković, Dejan, Soković, Marina, Zaghdoudi, Khalil, Barros, Lillian, Ferreira, Isabel C. F. R., "Recovery of Anthocyanins from Passion Fruit Epicarp for Food Colorants: Extraction Process Optimization and Evaluation of Bioactive Properties." in Molecules (Basel, Switzerland), 25, no. 14 (2020):3203,
https://doi.org/10.3390/molecules25143203 . .