TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5818
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
DO  - 10.3389/fevo.2023.1148309
SP  - 1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
doi = "10.3389/fevo.2023.1148309",
pages = "1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Ljaljević-Grbić, Milica
AU  - Stupar, Miloš
AU  - Vukojević, Jelena
AU  - Ćirić, Ana
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Vesović, Nikola
AU  - Živković, Nemanja
AU  - Ćurčić, Srećko
PY  - 2017
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4296
AB  - The antifungal potential of the pygidial gland secretion of the troglophilic ground beetle Laemostenus
punctatus from a cave in Southeastern Serbia against cavedwelling micromycetes, isolated from the same habitat, has
been investigated. Eleven collected samples were analyzed
and 32 isolates of cave-dwelling fungi were documented. A
total of 14 fungal species were identified as members of the
genera Aspergillus, Penicillium, Alternaria, Cladosporium,
Rhizopus, Trichoderma, Arthrinium, Aureobasidium,
Epicoccum, Talaromyces, and Fusarium. Five isolates were
selected for testing the antifungal activity of the pygidial gland
se cr etio n: Talaromyces d uclauxi, Aspergillus
brunneouniseriatus, Penicillium sp., Rhizopus stolonifer, and
Trichoderma viride. The microdilution method has been applied to detect minimal inhibitory concentrations (MICs) and
minimal fungicidal concentrations (MFCs). The most sensitive isolate was Penicillium sp., while the other isolates demonstrated a high level of resistance to the tested agent.
L. punctatus has developed a special mechanism of producing
specific compounds that act synergistically within the
secretion mixture, which are responsible for the antifungal
action against pathogens from the cave. The results open opportunities for further research in the field of ground beetle
defense against pathogens, which could have an important
application in human medicine, in addition to the environmental impact, primarily.
PB  - Berlin, Heidelberg: Springer-Verlag
T2  - The Science of Nature
T1  - Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes
IS  - 5-6
VL  - 104
DO  - 10.1007/s00114-017-1474-4
SP  - 52
ER  - 

@article{
author = "Nenadić, Marija and Ljaljević-Grbić, Milica and Stupar, Miloš and Vukojević, Jelena and Ćirić, Ana and Tešević, Vele and Vujisić, Ljubodrag and Todosijević, Marina and Vesović, Nikola and Živković, Nemanja and Ćurčić, Srećko",
year = "2017",
abstract = "The antifungal potential of the pygidial gland secretion of the troglophilic ground beetle Laemostenus
punctatus from a cave in Southeastern Serbia against cavedwelling micromycetes, isolated from the same habitat, has
been investigated. Eleven collected samples were analyzed
and 32 isolates of cave-dwelling fungi were documented. A
total of 14 fungal species were identified as members of the
genera Aspergillus, Penicillium, Alternaria, Cladosporium,
Rhizopus, Trichoderma, Arthrinium, Aureobasidium,
Epicoccum, Talaromyces, and Fusarium. Five isolates were
selected for testing the antifungal activity of the pygidial gland
se cr etio n: Talaromyces d uclauxi, Aspergillus
brunneouniseriatus, Penicillium sp., Rhizopus stolonifer, and
Trichoderma viride. The microdilution method has been applied to detect minimal inhibitory concentrations (MICs) and
minimal fungicidal concentrations (MFCs). The most sensitive isolate was Penicillium sp., while the other isolates demonstrated a high level of resistance to the tested agent.
L. punctatus has developed a special mechanism of producing
specific compounds that act synergistically within the
secretion mixture, which are responsible for the antifungal
action against pathogens from the cave. The results open opportunities for further research in the field of ground beetle
defense against pathogens, which could have an important
application in human medicine, in addition to the environmental impact, primarily.",
publisher = "Berlin, Heidelberg: Springer-Verlag",
journal = "The Science of Nature",
title = "Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes",
number = "5-6",
volume = "104",
doi = "10.1007/s00114-017-1474-4",
pages = "52"
}

Nenadić, M., Ljaljević-Grbić, M., Stupar, M., Vukojević, J., Ćirić, A., Tešević, V., Vujisić, L., Todosijević, M., Vesović, N., Živković, N.,& Ćurčić, S.. (2017). Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes. in The Science of Nature
Berlin, Heidelberg: Springer-Verlag., 104(5-6), 52.
https://doi.org/10.1007/s00114-017-1474-4

Nenadić M, Ljaljević-Grbić M, Stupar M, Vukojević J, Ćirić A, Tešević V, Vujisić L, Todosijević M, Vesović N, Živković N, Ćurčić S. Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes. in The Science of Nature. 2017;104(5-6):52.
doi:10.1007/s00114-017-1474-4 .

Nenadić, Marija, Ljaljević-Grbić, Milica, Stupar, Miloš, Vukojević, Jelena, Ćirić, Ana, Tešević, Vele, Vujisić, Ljubodrag, Todosijević, Marina, Vesović, Nikola, Živković, Nemanja, Ćurčić, Srećko, "Antifungal activity of the pygidial gland secretion of Laemostenus punctatus (Coleoptera: Carabidae) against cave-dwelling micromycetes" in The Science of Nature, 104, no. 5-6 (2017):52,
https://doi.org/10.1007/s00114-017-1474-4 . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Perić Mataruga, Vesna
AU  - Ilijin, Larisa
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2016
UR  - https://link.springer.com/article/10.1007/s00114-016-1358-z
UR  - https://pubmed.ncbi.nlm.nih.gov/27000261/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3981
AB  - The antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, Carabus ullrichii, C. coriaceus, and Abax parallelepipedus, have been tested. Microdilution method was applied for detection of minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), and minimal fungicidal concentrations (MFCs). Additionally, morpho-histology of the pygidial glands is investigated. We have tested 16 laboratory and clinical strains of human pathogens-eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples of C. ullrichii have showed the strongest antimicrobial effect against all strains of treated bacteria and fungi. Staphylococcus aureus, Lysteria monocytogenes, and Salmonella typhimurium proved to be the most sensitive bacterial strains. Penicillium funiculosum proved to be the most sensitive micromycete, while P. ochrochloron and P. verrucosum var. cyclopium the most resistant micromycetes. The pygidial secretion of C. coriaceus has showed antibacterial potential solely against Pseudomonas aeruginosa and antifungal activity against Aspergillus fumigatus, A. versicolor, A. ochraceus, and P. ochrochloron. Antibacterial properties of pygidial gland secretion of A. parallelepipedus were achieved against P. aeruginosa, while antifungal activity was detected against five of the eight tested micromycetes (A. fumigatus, A. versicolor, A. ochraceus, Trichoderma viride, and P. verrucosum var. cyclopium). Commercial antibiotics Streptomycin and Ampicillin and mycotics Ketoconazole and Bifonazole, applied as the positive controls, showed higher antibacterial/antifungal properties for all bacterial and fungal strains. The results of this observation might have a significant impact on the environmental aspects and possible medical purpose in the future.
PB  - Springer Nature
T2  - The Science of Nature
T1  - Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)
IS  - 3-4
VL  - 103
DO  - 10.1007/s00114-016-1358-z
SP  - 34
ER  - 

@article{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Perić Mataruga, Vesna and Ilijin, Larisa and Tešević, Vele and Vujisić, Ljubodrag and Todosijević, Marina and Vesović, Nikola and Ćurčić, Srećko",
year = "2016",
abstract = "The antimicrobial properties of the pygidial gland secretions released by the adults of the three ground beetle species, Carabus ullrichii, C. coriaceus, and Abax parallelepipedus, have been tested. Microdilution method was applied for detection of minimal inhibitory concentrations (MICs), minimal bactericidal concentrations (MBCs), and minimal fungicidal concentrations (MFCs). Additionally, morpho-histology of the pygidial glands is investigated. We have tested 16 laboratory and clinical strains of human pathogens-eight bacterial both gram-positive and gram-negative species and eight fungal species. The pygidial secretion samples of C. ullrichii have showed the strongest antimicrobial effect against all strains of treated bacteria and fungi. Staphylococcus aureus, Lysteria monocytogenes, and Salmonella typhimurium proved to be the most sensitive bacterial strains. Penicillium funiculosum proved to be the most sensitive micromycete, while P. ochrochloron and P. verrucosum var. cyclopium the most resistant micromycetes. The pygidial secretion of C. coriaceus has showed antibacterial potential solely against Pseudomonas aeruginosa and antifungal activity against Aspergillus fumigatus, A. versicolor, A. ochraceus, and P. ochrochloron. Antibacterial properties of pygidial gland secretion of A. parallelepipedus were achieved against P. aeruginosa, while antifungal activity was detected against five of the eight tested micromycetes (A. fumigatus, A. versicolor, A. ochraceus, Trichoderma viride, and P. verrucosum var. cyclopium). Commercial antibiotics Streptomycin and Ampicillin and mycotics Ketoconazole and Bifonazole, applied as the positive controls, showed higher antibacterial/antifungal properties for all bacterial and fungal strains. The results of this observation might have a significant impact on the environmental aspects and possible medical purpose in the future.",
publisher = "Springer Nature",
journal = "The Science of Nature",
title = "Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)",
number = "3-4",
volume = "103",
doi = "10.1007/s00114-016-1358-z",
pages = "34"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Perić Mataruga, V., Ilijin, L., Tešević, V., Vujisić, L., Todosijević, M., Vesović, N.,& Ćurčić, S.. (2016). Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae). in The Science of Nature
Springer Nature., 103(3-4), 34.
https://doi.org/10.1007/s00114-016-1358-z

Nenadić M, Soković M, Glamočlija J, Ćirić A, Perić Mataruga V, Ilijin L, Tešević V, Vujisić L, Todosijević M, Vesović N, Ćurčić S. Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae). in The Science of Nature. 2016;103(3-4):34.
doi:10.1007/s00114-016-1358-z .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Perić Mataruga, Vesna, Ilijin, Larisa, Tešević, Vele, Vujisić, Ljubodrag, Todosijević, Marina, Vesović, Nikola, Ćurčić, Srećko, "Antimicrobial activity of the pygidial gland secretion of three ground beetle species (Insecta: Coleoptera: Carabidae)" in The Science of Nature, 103, no. 3-4 (2016):34,
https://doi.org/10.1007/s00114-016-1358-z . .

TY  - JOUR
AU  - Nenadić, Marija
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ćirić, Ana
AU  - Perić Mataruga, Vesna
AU  - Tešević, Vele
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Vesović, Nikola
AU  - Ćurčić, Srećko
PY  - 2016
UR  - https://www.cambridge.org/core/journals/bulletin-of-entomological-research/article/antimicrobial-activity-of-the-pygidial-gland-secretion-of-the-troglophilic-ground-beetle-laemostenus-pristonychus-punctatus-dejean-1828-insecta-coleoptera-carabidae/8DF8DE1725619065F0C0C1631B5CF359
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3970
AB  - The antimicrobial activity of the pygidial gland secretion released by adult individuals
of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean,
1828), applying microdilution method with the aim to detect minimal inhibitory concentration,
minimal bactericidal concentration and minimal fungicidal concentration,
has been investigated. In addition, morphology of the pygidial glands is observed.
Wehave tested 16 laboratory and clinical strains of human pathogens – eight bacterial
both gram-positive and gram-negative species and eight fungal species. The pygidial
secretion samples have showed antimicrobial properties against all strains of treated
bacteria and fungi. Micrococcus flavus proved to be more resistant compared with
other bacterial strains. More significant antimicrobial properties of the secretion are
observed against Escherichia coli, which proved to be the most sensitive bacteria.
Aspergillus fumigatus proved to be the most resistant, while Penicillium ochrochloron and
Penicillium verrucosum var. cyclopium themost sensitive micromycetes. Commercial antibiotics
Streptomycin and Ampicillin and antimycotics Ketoconazole and Bifonazole, applied
as positive controls, showed higher antibacterial properties for all bacterial and
fungal strains, except for P. ochrochloron, which proved to be more resistant on
Ketoconazole compared with the pygidial gland secretion of L. (P.) punctatus. Apart
from the role in ecological aspects, the antimicrobial properties of the tested secretion
possibly might have medical significance in the future.
PB  - Cambridge University Press
T2  - Bulletin of Entomological Research
T1  - Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)
IS  - 4
VL  - 106
DO  - 10.1017/S0007485316000109
SP  - 474
EP  - 480
ER  - 

@article{
author = "Nenadić, Marija and Soković, Marina and Glamočlija, Jasmina and Ćirić, Ana and Perić Mataruga, Vesna and Tešević, Vele and Vujisić, Ljubodrag and Todosijević, Marina and Vesović, Nikola and Ćurčić, Srećko",
year = "2016",
abstract = "The antimicrobial activity of the pygidial gland secretion released by adult individuals
of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean,
1828), applying microdilution method with the aim to detect minimal inhibitory concentration,
minimal bactericidal concentration and minimal fungicidal concentration,
has been investigated. In addition, morphology of the pygidial glands is observed.
Wehave tested 16 laboratory and clinical strains of human pathogens – eight bacterial
both gram-positive and gram-negative species and eight fungal species. The pygidial
secretion samples have showed antimicrobial properties against all strains of treated
bacteria and fungi. Micrococcus flavus proved to be more resistant compared with
other bacterial strains. More significant antimicrobial properties of the secretion are
observed against Escherichia coli, which proved to be the most sensitive bacteria.
Aspergillus fumigatus proved to be the most resistant, while Penicillium ochrochloron and
Penicillium verrucosum var. cyclopium themost sensitive micromycetes. Commercial antibiotics
Streptomycin and Ampicillin and antimycotics Ketoconazole and Bifonazole, applied
as positive controls, showed higher antibacterial properties for all bacterial and
fungal strains, except for P. ochrochloron, which proved to be more resistant on
Ketoconazole compared with the pygidial gland secretion of L. (P.) punctatus. Apart
from the role in ecological aspects, the antimicrobial properties of the tested secretion
possibly might have medical significance in the future.",
publisher = "Cambridge University Press",
journal = "Bulletin of Entomological Research",
title = "Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)",
number = "4",
volume = "106",
doi = "10.1017/S0007485316000109",
pages = "474-480"
}

Nenadić, M., Soković, M., Glamočlija, J., Ćirić, A., Perić Mataruga, V., Tešević, V., Vujisić, L., Todosijević, M., Vesović, N.,& Ćurčić, S.. (2016). Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae). in Bulletin of Entomological Research
Cambridge University Press., 106(4), 474-480.
https://doi.org/10.1017/S0007485316000109

Nenadić M, Soković M, Glamočlija J, Ćirić A, Perić Mataruga V, Tešević V, Vujisić L, Todosijević M, Vesović N, Ćurčić S. Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae). in Bulletin of Entomological Research. 2016;106(4):474-480.
doi:10.1017/S0007485316000109 .

Nenadić, Marija, Soković, Marina, Glamočlija, Jasmina, Ćirić, Ana, Perić Mataruga, Vesna, Tešević, Vele, Vujisić, Ljubodrag, Todosijević, Marina, Vesović, Nikola, Ćurčić, Srećko, "Antimicrobial activity of the pygidial gland secretion of the troglophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) (Insecta: Coleoptera: Carabidae)" in Bulletin of Entomological Research, 106, no. 4 (2016):474-480,
https://doi.org/10.1017/S0007485316000109 . .

TY  - JOUR
AU  - Pavlović, Danica
AU  - Petković, Branka
AU  - Ćurčić, Srećko
AU  - Todorović, Dajana
AU  - Vesović, Nikola
AU  - Pantelić, Dejan
AU  - Perić Mataruga, Vesna
PY  - 2016
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1111/eea.12470
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3779
AB  - The aim of this study was to investigate the effects of a static magnetic field on six behavioral parameters (travel distance, average speed while in motion, travel distance of the head, body rotations, time in movement, and immobility time) of the ground beetle Laemostenus (Pristonychus) punctatus (Dejean) (Coleoptera: Carabidae). Adults of this troglophilic and guanophilic beetle were randomly divided into two groups, the first exposed to a static magnetic field of 110 mT for 5 h, and the second a control group. Beetle behavior after these 5 h was monitored in an open-field test for 12 min and analyzed using ANY-maze software. Exposure to a static magnetic field of 110 mT increased motor activity (travel distance and average speed while in motion) in the first 4 min. After that there were no significant differences. We conclude that the applied static magnetic field affects motor activity of adult specimens of L. (P.) punctatus, and we discuss the mechanism, possibly through acting on the control centers responsible for orientation and movement.
PB  - Blackwell Publishing Ltd.
T2  - Entomologia Experimentalis et Applicata
T1  - Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT
IS  - 2
VL  - 160
DO  - 10.1111/eea.12470
SP  - 188
EP  - 194
ER  - 

@article{
author = "Pavlović, Danica and Petković, Branka and Ćurčić, Srećko and Todorović, Dajana and Vesović, Nikola and Pantelić, Dejan and Perić Mataruga, Vesna",
year = "2016",
abstract = "The aim of this study was to investigate the effects of a static magnetic field on six behavioral parameters (travel distance, average speed while in motion, travel distance of the head, body rotations, time in movement, and immobility time) of the ground beetle Laemostenus (Pristonychus) punctatus (Dejean) (Coleoptera: Carabidae). Adults of this troglophilic and guanophilic beetle were randomly divided into two groups, the first exposed to a static magnetic field of 110 mT for 5 h, and the second a control group. Beetle behavior after these 5 h was monitored in an open-field test for 12 min and analyzed using ANY-maze software. Exposure to a static magnetic field of 110 mT increased motor activity (travel distance and average speed while in motion) in the first 4 min. After that there were no significant differences. We conclude that the applied static magnetic field affects motor activity of adult specimens of L. (P.) punctatus, and we discuss the mechanism, possibly through acting on the control centers responsible for orientation and movement.",
publisher = "Blackwell Publishing Ltd.",
journal = "Entomologia Experimentalis et Applicata",
title = "Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT",
number = "2",
volume = "160",
doi = "10.1111/eea.12470",
pages = "188-194"
}

Pavlović, D., Petković, B., Ćurčić, S., Todorović, D., Vesović, N., Pantelić, D.,& Perić Mataruga, V.. (2016). Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT. in Entomologia Experimentalis et Applicata
Blackwell Publishing Ltd.., 160(2), 188-194.
https://doi.org/10.1111/eea.12470

Pavlović D, Petković B, Ćurčić S, Todorović D, Vesović N, Pantelić D, Perić Mataruga V. Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT. in Entomologia Experimentalis et Applicata. 2016;160(2):188-194.
doi:10.1111/eea.12470 .

Pavlović, Danica, Petković, Branka, Ćurčić, Srećko, Todorović, Dajana, Vesović, Nikola, Pantelić, Dejan, Perić Mataruga, Vesna, "Increased motor activity of the beetle Laemostenus punctatus caused by a static magnetic field of 110 mT" in Entomologia Experimentalis et Applicata, 160, no. 2 (2016):188-194,
https://doi.org/10.1111/eea.12470 . .

TY  - CONF
AU  - Pavlović, Danica
AU  - Petković, Branka
AU  - Ćurčić, Srećko
AU  - Todorović, Dajana
AU  - Vesović, Nikola
AU  - Pantelić, Dejan
AU  - Perić Mataruga, Vesna
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4025
AB  - Uticaj magnetnog polja na žive sisteme od velikog je značaja i predmet je intenzivnog ispitivanja, naročito poslednjih godina u eri sve većeg elektromagnetnog zagađenja. Laemostenus punctatus (Dejean, 1828) je vrsta troglofilnog tvrdokrilca iz porodice trčuljaka (fam. Carabidae). Cilj ove studije je bio da se ispita efekat statičkog magnetnog polja na različite parametre ponašanja (pređeni put, prosečna brzina, pokreti glave, rotacije tela, vreme kretanja i vreme mirovanja) jedinki L. punctatus. U eksperimentu su korišćene adultne jedinke (n = 17) ove vrste izlovljene u martu 2015. godine u Ogoreličkoj pećini, selo Sićevo, u blizini Niša, jugoistočna Srbija. Jedinke su bile podeljene u dve grupe: kontrolna (n = 9) i eksperimentalna (n = 8). Eksperimentalna grupa jedinki izlagana je statičkom magnetnom polju (110 mT) u trajanju od 5 sati. Ponašanje kontrolnih i tretiranih jedinki, neposredno nakon izlaganja, je praćeno u „open field“ testu u trajanju od 12 minuta i automatski analizirano korišćenjem Any-maze programa. Rezultati su grafički prikazani u intervalima od po 4 minuta. Pokazano je da izlaganje statičkom magnetnom polju povećava motornu aktivnost (pređeni put, prosečna brzina, pokreti glave, rotacije tela i vreme kretanja) i stoga smanjuje vreme mirovanja jedinki L. punctatus. Najznačajnije promene za sve navedene parametre uočene su u prvih 4 minuta, pri čemu je statistička značajnost utvrđena za sledeća dva parametra: pređeni put i prosečna brzina (p<0,05; Mann-Whitney U test). Na osnovu dobijenih rezultata možemo zaključiti da statičko magnetno polje utiče na ponašanje adultnih jedinki vrste L. punctatus, odnosno da deluje na centre odgovorne za kontrolu motorne aktivnosti.
PB  - Belgrade: Entomological Society of Serbia
C3  - Simpozijum entomologa Srbije 2015
T1  - Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem
SP  - 17
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4025
ER  - 

@conference{
author = "Pavlović, Danica and Petković, Branka and Ćurčić, Srećko and Todorović, Dajana and Vesović, Nikola and Pantelić, Dejan and Perić Mataruga, Vesna",
year = "2015",
abstract = "Uticaj magnetnog polja na žive sisteme od velikog je značaja i predmet je intenzivnog ispitivanja, naročito poslednjih godina u eri sve većeg elektromagnetnog zagađenja. Laemostenus punctatus (Dejean, 1828) je vrsta troglofilnog tvrdokrilca iz porodice trčuljaka (fam. Carabidae). Cilj ove studije je bio da se ispita efekat statičkog magnetnog polja na različite parametre ponašanja (pređeni put, prosečna brzina, pokreti glave, rotacije tela, vreme kretanja i vreme mirovanja) jedinki L. punctatus. U eksperimentu su korišćene adultne jedinke (n = 17) ove vrste izlovljene u martu 2015. godine u Ogoreličkoj pećini, selo Sićevo, u blizini Niša, jugoistočna Srbija. Jedinke su bile podeljene u dve grupe: kontrolna (n = 9) i eksperimentalna (n = 8). Eksperimentalna grupa jedinki izlagana je statičkom magnetnom polju (110 mT) u trajanju od 5 sati. Ponašanje kontrolnih i tretiranih jedinki, neposredno nakon izlaganja, je praćeno u „open field“ testu u trajanju od 12 minuta i automatski analizirano korišćenjem Any-maze programa. Rezultati su grafički prikazani u intervalima od po 4 minuta. Pokazano je da izlaganje statičkom magnetnom polju povećava motornu aktivnost (pređeni put, prosečna brzina, pokreti glave, rotacije tela i vreme kretanja) i stoga smanjuje vreme mirovanja jedinki L. punctatus. Najznačajnije promene za sve navedene parametre uočene su u prvih 4 minuta, pri čemu je statistička značajnost utvrđena za sledeća dva parametra: pređeni put i prosečna brzina (p<0,05; Mann-Whitney U test). Na osnovu dobijenih rezultata možemo zaključiti da statičko magnetno polje utiče na ponašanje adultnih jedinki vrste L. punctatus, odnosno da deluje na centre odgovorne za kontrolu motorne aktivnosti.",
publisher = "Belgrade: Entomological Society of Serbia",
journal = "Simpozijum entomologa Srbije 2015",
title = "Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem",
pages = "17",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4025"
}

Pavlović, D., Petković, B., Ćurčić, S., Todorović, D., Vesović, N., Pantelić, D.,& Perić Mataruga, V.. (2015). Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem. in Simpozijum entomologa Srbije 2015
Belgrade: Entomological Society of Serbia., 17.
https://hdl.handle.net/21.15107/rcub_ibiss_4025

Pavlović D, Petković B, Ćurčić S, Todorović D, Vesović N, Pantelić D, Perić Mataruga V. Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem. in Simpozijum entomologa Srbije 2015. 2015;:17.
https://hdl.handle.net/21.15107/rcub_ibiss_4025 .

Pavlović, Danica, Petković, Branka, Ćurčić, Srećko, Todorović, Dajana, Vesović, Nikola, Pantelić, Dejan, Perić Mataruga, Vesna, "Hipermotorno ponašanje vrste Laemostenus punctatus (Dejean, 1828) (Coleoptera: Carabidae) izazvano statičkim magnetnim poljem" in Simpozijum entomologa Srbije 2015 (2015):17,
https://hdl.handle.net/21.15107/rcub_ibiss_4025 .