TY  - JOUR
AU  - Đorđevski, Nikoleta
AU  - Uba, Abdullahi Ibrahim
AU  - Zengin, Gokhan
AU  - Božunović, Jelena
AU  - Gašić, Uroš
AU  - Ristanović, Elizabeta
AU  - Ćirić, Ana
AU  - Nikolić, Biljana
AU  - Stojković, Dejan
PY  - 2023
UR  - https://www.mdpi.com/1424-8247/16/1/21
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9865742
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5433
AB  - This study was designed to investigate the impact of different extraction solvent systems on the chemical composition and biological activities of Allium scorodoprasum L. (Amaryllidaceae)-the medicinal plant that was traditionally used as a remedy in the medieval period in the Balkans. Targeted chemical analysis of nine different extracts was performed by UHPLC(-)HESI-QqQ-MS/MS. Antimicrobial and antibiofilm activities of the extracts were investigated on sixteen clinical isolates of bacteria, yeasts and dermatomycetes, all isolated from infected human skin and corneal formations. Cytotoxicity and wound-healing properties were tested on human immortalized keratinocytes (HaCaT cell line). Antioxidant activity was assessed by six different assays, while beneficial potential against certain neurodegenerative diseases and type 2 diabetes was determined in selected enzyme inhibition assays coupled with molecular modeling. The results showed that the obtained extracts were rich in phenolic compounds, especially flavonoid glycosides such as rutin and kaempferol 3-O-glucoside. All of the extracts showed antimicrobial, wound-healing, antioxidant and anti-enzymatic properties. This study is the first of its kind, linking the medieval medicinal use of wild-growing flowers of A. scorodoprasum with contemporary in vitro scientific approaches.
PB  - Basel: MDPI
T2  - Pharmaceuticals (Basel, Switzerland)
T1  - Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts
IS  - 1
VL  - 16
DO  - 10.3390/ph16010021
SP  - 21
ER  - 

@article{
author = "Đorđevski, Nikoleta and Uba, Abdullahi Ibrahim and Zengin, Gokhan and Božunović, Jelena and Gašić, Uroš and Ristanović, Elizabeta and Ćirić, Ana and Nikolić, Biljana and Stojković, Dejan",
year = "2023",
abstract = "This study was designed to investigate the impact of different extraction solvent systems on the chemical composition and biological activities of Allium scorodoprasum L. (Amaryllidaceae)-the medicinal plant that was traditionally used as a remedy in the medieval period in the Balkans. Targeted chemical analysis of nine different extracts was performed by UHPLC(-)HESI-QqQ-MS/MS. Antimicrobial and antibiofilm activities of the extracts were investigated on sixteen clinical isolates of bacteria, yeasts and dermatomycetes, all isolated from infected human skin and corneal formations. Cytotoxicity and wound-healing properties were tested on human immortalized keratinocytes (HaCaT cell line). Antioxidant activity was assessed by six different assays, while beneficial potential against certain neurodegenerative diseases and type 2 diabetes was determined in selected enzyme inhibition assays coupled with molecular modeling. The results showed that the obtained extracts were rich in phenolic compounds, especially flavonoid glycosides such as rutin and kaempferol 3-O-glucoside. All of the extracts showed antimicrobial, wound-healing, antioxidant and anti-enzymatic properties. This study is the first of its kind, linking the medieval medicinal use of wild-growing flowers of A. scorodoprasum with contemporary in vitro scientific approaches.",
publisher = "Basel: MDPI",
journal = "Pharmaceuticals (Basel, Switzerland)",
title = "Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts",
number = "1",
volume = "16",
doi = "10.3390/ph16010021",
pages = "21"
}

Đorđevski, N., Uba, A. I., Zengin, G., Božunović, J., Gašić, U., Ristanović, E., Ćirić, A., Nikolić, B.,& Stojković, D.. (2023). Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts. in Pharmaceuticals (Basel, Switzerland)
Basel: MDPI., 16(1), 21.
https://doi.org/10.3390/ph16010021

Đorđevski N, Uba AI, Zengin G, Božunović J, Gašić U, Ristanović E, Ćirić A, Nikolić B, Stojković D. Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts. in Pharmaceuticals (Basel, Switzerland). 2023;16(1):21.
doi:10.3390/ph16010021 .

Đorđevski, Nikoleta, Uba, Abdullahi Ibrahim, Zengin, Gokhan, Božunović, Jelena, Gašić, Uroš, Ristanović, Elizabeta, Ćirić, Ana, Nikolić, Biljana, Stojković, Dejan, "Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts" in Pharmaceuticals (Basel, Switzerland), 16, no. 1 (2023):21,
https://doi.org/10.3390/ph16010021 . .

TY  - JOUR
AU  - Đorđevski, Nikoleta
AU  - Stojković, Dejan
AU  - Živković, Jelena
AU  - Pljevljakušić, Dejan
AU  - Ristanović, Elizabeta
AU  - Nikolić, Biljana
AU  - Ćirić, Ana
PY  - 2022
UR  - https://onlinelibrary.wiley.com/doi/10.1002/fsn3.2777
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4864
AB  - This study was designed to explore functional food properties of edible seed oil obtained from Tamjanika seeds—autochthonous grape variety of Balkan Peninsula. In order to accomplish our goals, seed oil was isolated by Soxhlet apparatus and chemically characterized regarding fatty acids, carotenoids, tocopherols, and tocotrienols. Antimicrobial activity of the isolated oil was tested by microdilution method. For that purposes, six bacterial species were used, belonging to human infectious agents and food contaminants. Furthermore, the activity of the oil was investigated against clinical isolates of dermatomycetes. Our study has shown that oil of Vitis vinifera L. Tamjanika variety was an abundant source of polyunsaturated fatty acids (81.43%) with predominant linoleic acid. HPLC analysis revealed the presence of carotenoid lutein (0.15 mg/100 g). The seed oil was rich in tocotrienols (85.04 mg/100 g) predominating over tocopherols (8.37 mg/100 g). The oil possessed microbicidal activity against all the tested microbes. Bacteria were more sensitive to the effect of the oil (minimum inhibitory concentration [MIC] 7.7–15.4) when compared with oil effect on tested dermatomycetes (MIC 20–40). Our investigation has shown for the first time that grape oil could be active against wide spectrum of bacteria and clinically isolated dermatomycetes. The significance of this study lies in the fact that it pointed out the functional food properties of grape seed oil that was fully chemically characterized.
PB  - John Wiley and Sons Inc
T2  - Food Science & Nutrition
T1  - Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil
IS  - 4
VL  - 10
DO  - 10.1002/fsn3.2777
SP  - 1312
EP  - 1319
ER  - 

@article{
author = "Đorđevski, Nikoleta and Stojković, Dejan and Živković, Jelena and Pljevljakušić, Dejan and Ristanović, Elizabeta and Nikolić, Biljana and Ćirić, Ana",
year = "2022",
abstract = "This study was designed to explore functional food properties of edible seed oil obtained from Tamjanika seeds—autochthonous grape variety of Balkan Peninsula. In order to accomplish our goals, seed oil was isolated by Soxhlet apparatus and chemically characterized regarding fatty acids, carotenoids, tocopherols, and tocotrienols. Antimicrobial activity of the isolated oil was tested by microdilution method. For that purposes, six bacterial species were used, belonging to human infectious agents and food contaminants. Furthermore, the activity of the oil was investigated against clinical isolates of dermatomycetes. Our study has shown that oil of Vitis vinifera L. Tamjanika variety was an abundant source of polyunsaturated fatty acids (81.43%) with predominant linoleic acid. HPLC analysis revealed the presence of carotenoid lutein (0.15 mg/100 g). The seed oil was rich in tocotrienols (85.04 mg/100 g) predominating over tocopherols (8.37 mg/100 g). The oil possessed microbicidal activity against all the tested microbes. Bacteria were more sensitive to the effect of the oil (minimum inhibitory concentration [MIC] 7.7–15.4) when compared with oil effect on tested dermatomycetes (MIC 20–40). Our investigation has shown for the first time that grape oil could be active against wide spectrum of bacteria and clinically isolated dermatomycetes. The significance of this study lies in the fact that it pointed out the functional food properties of grape seed oil that was fully chemically characterized.",
publisher = "John Wiley and Sons Inc",
journal = "Food Science & Nutrition",
title = "Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil",
number = "4",
volume = "10",
doi = "10.1002/fsn3.2777",
pages = "1312-1319"
}

Đorđevski, N., Stojković, D., Živković, J., Pljevljakušić, D., Ristanović, E., Nikolić, B.,& Ćirić, A.. (2022). Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil. in Food Science & Nutrition
John Wiley and Sons Inc., 10(4), 1312-1319.
https://doi.org/10.1002/fsn3.2777

Đorđevski N, Stojković D, Živković J, Pljevljakušić D, Ristanović E, Nikolić B, Ćirić A. Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil. in Food Science & Nutrition. 2022;10(4):1312-1319.
doi:10.1002/fsn3.2777 .

Đorđevski, Nikoleta, Stojković, Dejan, Živković, Jelena, Pljevljakušić, Dejan, Ristanović, Elizabeta, Nikolić, Biljana, Ćirić, Ana, "Tamjanika, a Balkan native variety of Vitis vinifera L.: Chemical characterization, antibacterial, and anti‐dermatomycosis potential of seed oil" in Food Science & Nutrition, 10, no. 4 (2022):1312-1319,
https://doi.org/10.1002/fsn3.2777 . .

TY  - CONF
AU  - Đorđevski, Nikoleta
AU  - Ćirić, Ana
AU  - Nikolić, Biljana
AU  - Stojković, Dejan
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5580
AB  - Ова студија дизајнирана је како би се испитао биолошки потенцијал ектраката
цветова Allium scorodoprasum на одређене изолате дерматомицета. Направљено је
девет различитих екстраката кoји су хемијски окарактерисани. Антимикробна
активност испитиваних екстраката тестирана је микродилуционом методом.
Коришћено је десет клиничких изолата дерматомицета изолованих са коже
пацијената (Trichophyton tonsurans, Trichophyton megnini, Trichophyton
mentagrophytes два изолата, Trichophyton rubrum, Microsporum canis два изолата,
Microsporum audouinii два изолата, Epidermophyton floccosum). Екстракти су били
ефикасни против свих тестираних гљива. Најбољу активност према свим
тестираним дерматомицетама показао је екстракт етанол:вода (50:50, v/v). Врсте
рода Microsporum су биле генерално најосетљивије на дејство екстраката. Како
постоји повећана отпорност хуманих патогена на постојеће комерцијалне лекове и
како конвенционални третмани могу имати многа нежељена дејства, у једном
тренутку овакав приступ могао би постати недовољан за лечење. Због овакве
ситуације развој ефикаснијих и мање токсичних антимикробних агенаса,
укључујући природне производе, од круцијалног је значаја. С обзиром да су
екстракти цветова Allium scorodoprasum in vitro показали значајан антифунгални
потенцијал, препоручљиво је њихово даље истраживање у in vivo студијама, а
свакако су потребне и даље клиничке студије.
AB  - Ova studija dizajnirana je kako bi se ispitao biološki potencijal ektrakata cvetova Allium scorodoprasum na određene izolate dermatomiceta. Napravljeno je devet različitih ekstrakata koji su hemijski okarakterisani. Antimikrobna aktivnost ispitivanih ekstrakata testirana je mikrodilucionom metodom. Korišćeno je deset kliničkih izolata dermatomiceta izolovanih sa kože pacijenata (Trichophyton tonsurans, Trichophyton megnini, Trichophyton mentagrophytes dva izolata, Trichophyton rubrum, Microsporum canis dva izolata, Microsporum audouinii dva izolata, Epidermophyton floccosum). Ekstrakti su bili efikasni protiv svih testiranih gljiva. Najbolju aktivnost prema svim testiranim dermatomicetama pokazao je ekstrakt etanol:voda (50:50, v/v). Vrste roda Microsporum su bile generalno najosetljivije na dejstvo ekstrakata. Kako postoji povećana otpornost humanih patogena na postojeće komercijalne lekove i kako konvencionalni tretmani mogu imati mnoga neželjena dejstva, u jednom trenutku ovakav pristup mogao bi postati nedovoljan za lečenje. Zbog ovakve situacije razvoj efikasnijih i manje toksičnih antimikrobnih agenasa, uključujući prirodne proizvode, od krucijalnog je značaja. S obzirom da su ekstrakti cvetova Allium scorodoprasum in vitro pokazali značajan antifungalni potencijal, preporučljivo je njihovo dalje istraživanje u in vivo studijama, a svakako su potrebne i dalje kliničke studije.
PB  - Belgrade: Serbian Biological Society
C3  - Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
T1  - Антифунгални потенцијал екстраката цветова Allium scorodoprasum на хумане изолате дерматомицета
T1  - Antifungalni potencijal ekstrakata cvetova Allium scorodoprasum na humane izolate dermatomiceta
SP  - 270
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5580
ER  - 

@conference{
author = "Đorđevski, Nikoleta and Ćirić, Ana and Nikolić, Biljana and Stojković, Dejan",
year = "2022",
abstract = "Ова студија дизајнирана је како би се испитао биолошки потенцијал ектраката
цветова Allium scorodoprasum на одређене изолате дерматомицета. Направљено је
девет различитих екстраката кoји су хемијски окарактерисани. Антимикробна
активност испитиваних екстраката тестирана је микродилуционом методом.
Коришћено је десет клиничких изолата дерматомицета изолованих са коже
пацијената (Trichophyton tonsurans, Trichophyton megnini, Trichophyton
mentagrophytes два изолата, Trichophyton rubrum, Microsporum canis два изолата,
Microsporum audouinii два изолата, Epidermophyton floccosum). Екстракти су били
ефикасни против свих тестираних гљива. Најбољу активност према свим
тестираним дерматомицетама показао је екстракт етанол:вода (50:50, v/v). Врсте
рода Microsporum су биле генерално најосетљивије на дејство екстраката. Како
постоји повећана отпорност хуманих патогена на постојеће комерцијалне лекове и
како конвенционални третмани могу имати многа нежељена дејства, у једном
тренутку овакав приступ могао би постати недовољан за лечење. Због овакве
ситуације развој ефикаснијих и мање токсичних антимикробних агенаса,
укључујући природне производе, од круцијалног је значаја. С обзиром да су
екстракти цветова Allium scorodoprasum in vitro показали значајан антифунгални
потенцијал, препоручљиво је њихово даље истраживање у in vivo студијама, а
свакако су потребне и даље клиничке студије., Ova studija dizajnirana je kako bi se ispitao biološki potencijal ektrakata cvetova Allium scorodoprasum na određene izolate dermatomiceta. Napravljeno je devet različitih ekstrakata koji su hemijski okarakterisani. Antimikrobna aktivnost ispitivanih ekstrakata testirana je mikrodilucionom metodom. Korišćeno je deset kliničkih izolata dermatomiceta izolovanih sa kože pacijenata (Trichophyton tonsurans, Trichophyton megnini, Trichophyton mentagrophytes dva izolata, Trichophyton rubrum, Microsporum canis dva izolata, Microsporum audouinii dva izolata, Epidermophyton floccosum). Ekstrakti su bili efikasni protiv svih testiranih gljiva. Najbolju aktivnost prema svim testiranim dermatomicetama pokazao je ekstrakt etanol:voda (50:50, v/v). Vrste roda Microsporum su bile generalno najosetljivije na dejstvo ekstrakata. Kako postoji povećana otpornost humanih patogena na postojeće komercijalne lekove i kako konvencionalni tretmani mogu imati mnoga neželjena dejstva, u jednom trenutku ovakav pristup mogao bi postati nedovoljan za lečenje. Zbog ovakve situacije razvoj efikasnijih i manje toksičnih antimikrobnih agenasa, uključujući prirodne proizvode, od krucijalnog je značaja. S obzirom da su ekstrakti cvetova Allium scorodoprasum in vitro pokazali značajan antifungalni potencijal, preporučljivo je njihovo dalje istraživanje u in vivo studijama, a svakako su potrebne i dalje kliničke studije.",
publisher = "Belgrade: Serbian Biological Society",
journal = "Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia",
title = "Антифунгални потенцијал екстраката цветова Allium scorodoprasum на хумане изолате дерматомицета, Antifungalni potencijal ekstrakata cvetova Allium scorodoprasum na humane izolate dermatomiceta",
pages = "270",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5580"
}

Đorđevski, N., Ćirić, A., Nikolić, B.,& Stojković, D.. (2022). Антифунгални потенцијал екстраката цветова Allium scorodoprasum на хумане изолате дерматомицета. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia
Belgrade: Serbian Biological Society., 270.
https://hdl.handle.net/21.15107/rcub_ibiss_5580

Đorđevski N, Ćirić A, Nikolić B, Stojković D. Антифунгални потенцијал екстраката цветова Allium scorodoprasum на хумане изолате дерматомицета. in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia. 2022;:270.
https://hdl.handle.net/21.15107/rcub_ibiss_5580 .

Đorđevski, Nikoleta, Ćirić, Ana, Nikolić, Biljana, Stojković, Dejan, "Антифунгални потенцијал екстраката цветова Allium scorodoprasum на хумане изолате дерматомицета" in Knjiga sažetaka: Treći Kongres biologa Srbije: Osnovna i primenjena istraživanja: Metodika nastave; 2022 Sep 21-25; Zlatibor, Serbia (2022):270,
https://hdl.handle.net/21.15107/rcub_ibiss_5580 .