TY  - JOUR
AU  - Nenadić, Marija
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Dimkić, Ivica
AU  - Janakiev, Tamara
AU  - Vesović, Nikola
AU  - Vujisić, Ljubodrag
AU  - Todosijević, Marina
AU  - Stanković, Saša S
AU  - Ćurčić, Nina B.
AU  - Milinčić, Uroš
AU  - Petrović, Dragan
AU  - Milinčić, Miroljub
AU  - Ćurčić, Srećko
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5818
AB  - In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).
PB  - Frontiers Media S.A.
T2  - Frontiers in Ecology and Evolution
T1  - The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.
VL  - 11
DO  - 10.3389/fevo.2023.1148309
SP  - 1148309
ER  - 

@article{
author = "Nenadić, Marija and Stojković, Dejan and Soković, Marina and Ćirić, Ana and Dimkić, Ivica and Janakiev, Tamara and Vesović, Nikola and Vujisić, Ljubodrag and Todosijević, Marina and Stanković, Saša S and Ćurčić, Nina B. and Milinčić, Uroš and Petrović, Dragan and Milinčić, Miroljub and Ćurčić, Srećko",
year = "2023",
abstract = "In the present study, we investigated in vitro the antimicrobial activity of the pygidial gland secretion of the guanophilic ground beetle Laemostenus (Pristonychus) punctatus (Dejean, 1828) and some of its chemicals against resistant and non-resistant bacteria and Candida species, the synergistic and additive potential of combinations of selected chemicals and antimicrobial drugs against resistant bacterial and fungal strains, anti-adhesive and anti-invasive potential of the secretion and formic acid alone and in selected combinations with antimicrobial drugs against methicillin-resistant Staphylococcus aureus (MRSA) toward spontaneously immortalized human keratinocyte cell line (HaCaT cells). In addition, we examined the antiproliferative activity of the secretion and formic acid in vitro. The tested secretion and the standards of formic and oleic acids possessed a significant level of antimicrobial potential against all tested strains (P < 0.05). The isolate from guano Pseudomonas monteilii showed the highest resistance to the secretion and formic acid, while MRSA achieved a significantly high level of susceptibility to all agents tested, particularly to the combinations of formic acid and antibiotics, but at the same time showed a certain level of resistance to the antibiotics tested individually. Candida albicans and C. tropicalis were found to be the most sensitive fungal strains to the secretion. Formic acid (MIC 0.0005 mg/mL) and gentamicin (MIC 0.0010 mg/mL) in the mixture achieved synergistic antibacterial activity against MRSA (FICI = 0.5, P < 0.05). The combination of formic acid, gentamicin and ampicillin accomplished an additive effect against this resistant bacterial strain (FICI = 1.5, P < 0.05). The secretion achieved a better inhibitory effect on the adhesion ability of MRSA toward HaCaT cells compared to formic acid alone, while formic acid showed better results regarding the invasion (P < 0.001). The combinations of gentamicin and ampicillin, as well as of formic acid and gentamicin and ampicillin achieved similar anti-adhesive and anti-invasive effects, with a slight advantage of formic acid and antibiotics in combination (P < 0.001). The secretion and formic acid were found to be non-toxic to HaCaT cells in vitro (IC50 ≥ 401 μg/mL).",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Ecology and Evolution",
title = "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.",
volume = "11",
doi = "10.3389/fevo.2023.1148309",
pages = "1148309"
}

Nenadić, M., Stojković, D., Soković, M., Ćirić, A., Dimkić, I., Janakiev, T., Vesović, N., Vujisić, L., Todosijević, M., Stanković, S. S., Ćurčić, N. B., Milinčić, U., Petrović, D., Milinčić, M.,& Ćurčić, S.. (2023). The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution
Frontiers Media S.A.., 11, 1148309.
https://doi.org/10.3389/fevo.2023.1148309

Nenadić M, Stojković D, Soković M, Ćirić A, Dimkić I, Janakiev T, Vesović N, Vujisić L, Todosijević M, Stanković SS, Ćurčić NB, Milinčić U, Petrović D, Milinčić M, Ćurčić S. The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities.. in Frontiers in Ecology and Evolution. 2023;11:1148309.
doi:10.3389/fevo.2023.1148309 .

Nenadić, Marija, Stojković, Dejan, Soković, Marina, Ćirić, Ana, Dimkić, Ivica, Janakiev, Tamara, Vesović, Nikola, Vujisić, Ljubodrag, Todosijević, Marina, Stanković, Saša S, Ćurčić, Nina B., Milinčić, Uroš, Petrović, Dragan, Milinčić, Miroljub, Ćurčić, Srećko, "The pygidial gland secretion of Laemostenus punctatus (Coleoptera, Carabidae): a source of natural agents with antimicrobial, anti-adhesive, and anti-invasive activities." in Frontiers in Ecology and Evolution, 11 (2023):1148309,
https://doi.org/10.3389/fevo.2023.1148309 . .

TY  - JOUR
AU  - Urošević, Aleksandar
AU  - Maričić, Marko
AU  - Vučić, Tijana
AU  - Žikić, Vladimir
AU  - Stanković, Saša S
AU  - Šević, Mirko
AU  - Anđelković, Marko
PY  - 2021
UR  - https://journal.pmf.ni.ac.rs/bionys/index.php/bionys/article/view/428
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4935
AB  - Kotschy's gecko (Mediodactylus kotschyi) is native to the eastern Mediterranean basin but well known as a successful colonizer. The most probable way of its spreading is cargo and passenger traffic. In Serbia, M. kotschyi is assumed native only in Prizren. Anthropogenic introductions were confirmed in Belgrade, Novi Sad, Pančevo and Smederevo. This study aimed to summarize data of M. kotschyi findings with reference to recently confirmed population in Niš and newly recorded population in Sremska Mitrovica. These two populations are well-established with multiple pieces of evidence of reproduction. Pholidosis data were collected for several individuals to confirm their specific status. New and previously recorded introduced populations of Kotchy's gecko in Serbia belong to M. kotschyi bibroni based on morphology. The introduction was most likely related to railways and further spread in urban zones is expected. It seems that introduced populations do not threaten native ecosystems as these populations are appeared to be highly localized in urban habitats. Apstrakt: Novi nalazi Kočijevog gekona, Mediodactylus kotschyi (Steindachner, 1870) u Srbiji, sa posebnim fokusom na nedavno zabeležene populacije u Nišu i Sremskoj Mitrovici Kočijev gekon (Mediodactylus kotschyi) se autohtono javlja u basenu istočnog Sredozemlja ali je dobro poznat i kao uspešan kolonizator. Najverovatniji načini njegovog širenja su teretni i putnički saobraćaj. U Srbiji, Kočijev gekon se smatra autohtonim samo na području Prizrena. Antropogene introdukcije potvrđene su u Beogradu, Novom Sadu, Pančevu i Smederevu. Cilj ove studije je da sumira podatke o nalazima M. kotschyi sa posebnom pažnjom na nedavno potvrđeno prisustvo populacije u Nišu i
T2  - Biologica Nyssana
T1  - New findings of Kotschy’s gecko, Mediodactylus kotschyi (Steindachner, 1870) in Serbia, with a particular focus on recently detected populations in Niš and Sremska Mitrovica
IS  - 2
VL  - 12
DO  - 10.5281/zenodo.5763846
SP  - 151
EP  - 157
ER  - 

@article{
author = "Urošević, Aleksandar and Maričić, Marko and Vučić, Tijana and Žikić, Vladimir and Stanković, Saša S and Šević, Mirko and Anđelković, Marko",
year = "2021",
abstract = "Kotschy's gecko (Mediodactylus kotschyi) is native to the eastern Mediterranean basin but well known as a successful colonizer. The most probable way of its spreading is cargo and passenger traffic. In Serbia, M. kotschyi is assumed native only in Prizren. Anthropogenic introductions were confirmed in Belgrade, Novi Sad, Pančevo and Smederevo. This study aimed to summarize data of M. kotschyi findings with reference to recently confirmed population in Niš and newly recorded population in Sremska Mitrovica. These two populations are well-established with multiple pieces of evidence of reproduction. Pholidosis data were collected for several individuals to confirm their specific status. New and previously recorded introduced populations of Kotchy's gecko in Serbia belong to M. kotschyi bibroni based on morphology. The introduction was most likely related to railways and further spread in urban zones is expected. It seems that introduced populations do not threaten native ecosystems as these populations are appeared to be highly localized in urban habitats. Apstrakt: Novi nalazi Kočijevog gekona, Mediodactylus kotschyi (Steindachner, 1870) u Srbiji, sa posebnim fokusom na nedavno zabeležene populacije u Nišu i Sremskoj Mitrovici Kočijev gekon (Mediodactylus kotschyi) se autohtono javlja u basenu istočnog Sredozemlja ali je dobro poznat i kao uspešan kolonizator. Najverovatniji načini njegovog širenja su teretni i putnički saobraćaj. U Srbiji, Kočijev gekon se smatra autohtonim samo na području Prizrena. Antropogene introdukcije potvrđene su u Beogradu, Novom Sadu, Pančevu i Smederevu. Cilj ove studije je da sumira podatke o nalazima M. kotschyi sa posebnom pažnjom na nedavno potvrđeno prisustvo populacije u Nišu i",
journal = "Biologica Nyssana",
title = "New findings of Kotschy’s gecko, Mediodactylus kotschyi (Steindachner, 1870) in Serbia, with a particular focus on recently detected populations in Niš and Sremska Mitrovica",
number = "2",
volume = "12",
doi = "10.5281/zenodo.5763846",
pages = "151-157"
}

Urošević, A., Maričić, M., Vučić, T., Žikić, V., Stanković, S. S., Šević, M.,& Anđelković, M.. (2021). New findings of Kotschy’s gecko, Mediodactylus kotschyi (Steindachner, 1870) in Serbia, with a particular focus on recently detected populations in Niš and Sremska Mitrovica. in Biologica Nyssana, 12(2), 151-157.
https://doi.org/10.5281/zenodo.5763846

Urošević A, Maričić M, Vučić T, Žikić V, Stanković SS, Šević M, Anđelković M. New findings of Kotschy’s gecko, Mediodactylus kotschyi (Steindachner, 1870) in Serbia, with a particular focus on recently detected populations in Niš and Sremska Mitrovica. in Biologica Nyssana. 2021;12(2):151-157.
doi:10.5281/zenodo.5763846 .

Urošević, Aleksandar, Maričić, Marko, Vučić, Tijana, Žikić, Vladimir, Stanković, Saša S, Šević, Mirko, Anđelković, Marko, "New findings of Kotschy’s gecko, Mediodactylus kotschyi (Steindachner, 1870) in Serbia, with a particular focus on recently detected populations in Niš and Sremska Mitrovica" in Biologica Nyssana, 12, no. 2 (2021):151-157,
https://doi.org/10.5281/zenodo.5763846 . .