TY  - CONF
AU  - Sarić, Ana
AU  - Rajić, Jovana
AU  - Tolić, Anja
AU  - Đorđević, Marija
AU  - Grdović, Nevena
AU  - Uskoković, Aleksandra
AU  - Dinić, Svetlana
AU  - Arambašić Jovanović, Jelena
AU  - Mihailović, Mirjana
AU  - Vidaković, Melita
AU  - Dučić, Tanja
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6304
AB  - Introduction: DNA methylation is a major regulator of transcriptional activity and alongside other epigenetic modifications it introduces specific level of chromatin complexity. Fourier transform infrared (FTIR) spectroscopy is a rapid, non-destructive, and label-free technique for identifying subtle changes in all bio-macromolecules, and it has been used as a method of choice for studying DNA conformation. The present study was designed to explore the use of synchrotron-based FTIR spectroscopy to monitor the subtle changes on molecular level regarding the DNA methylation status of cytosine in the whole genome.
Methods: For FTIR-based DNA methylation analysis in situ, DNA-HALO samples were prepared using slightly modified methodology for nuclear HALO preparations where DNA-HALOs are liberated of any protein residues but preserve higher order chromatin structure.
Results: Using FTIR spectroscopy we analysed and compared methylation profiles of isolated genomic DNA and DNA-HALO samples. DNA-HALO structure shows more distinct peaks in fingerprint region of spectra. DNA-HALO structure is more accurate for detecting bonds in unmemthylated cytosine as specific infrared peaks are defined as vibrations of bonds in unmethylated cytosine at 1151 cm-1 and 1357 cm-1. The ratio of integrated area under the peak 1151 cm-1 over integrated area under the peak that represents PO2-backbone vibrations can be used to assess level of unmethylated cytosine and thus methylation rate in the DNA-HALO samples.
Conclusion: This study demonstrates potential of FTIR spectroscopy to detect DNA methylation in DNA-HALO samples more precisely compared to classical DNA extraction procedure that yield unstructured whole genomic DNA.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
C3  - Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
T1  - Methylation profile analysis of Of DNA-HALO structure by synchrotron-based FTIR spectroscopy
SP  - 68
EP  - 68
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6304
ER  - 

@conference{
author = "Sarić, Ana and Rajić, Jovana and Tolić, Anja and Đorđević, Marija and Grdović, Nevena and Uskoković, Aleksandra and Dinić, Svetlana and Arambašić Jovanović, Jelena and Mihailović, Mirjana and Vidaković, Melita and Dučić, Tanja",
year = "2023",
abstract = "Introduction: DNA methylation is a major regulator of transcriptional activity and alongside other epigenetic modifications it introduces specific level of chromatin complexity. Fourier transform infrared (FTIR) spectroscopy is a rapid, non-destructive, and label-free technique for identifying subtle changes in all bio-macromolecules, and it has been used as a method of choice for studying DNA conformation. The present study was designed to explore the use of synchrotron-based FTIR spectroscopy to monitor the subtle changes on molecular level regarding the DNA methylation status of cytosine in the whole genome.
Methods: For FTIR-based DNA methylation analysis in situ, DNA-HALO samples were prepared using slightly modified methodology for nuclear HALO preparations where DNA-HALOs are liberated of any protein residues but preserve higher order chromatin structure.
Results: Using FTIR spectroscopy we analysed and compared methylation profiles of isolated genomic DNA and DNA-HALO samples. DNA-HALO structure shows more distinct peaks in fingerprint region of spectra. DNA-HALO structure is more accurate for detecting bonds in unmemthylated cytosine as specific infrared peaks are defined as vibrations of bonds in unmethylated cytosine at 1151 cm-1 and 1357 cm-1. The ratio of integrated area under the peak 1151 cm-1 over integrated area under the peak that represents PO2-backbone vibrations can be used to assess level of unmethylated cytosine and thus methylation rate in the DNA-HALO samples.
Conclusion: This study demonstrates potential of FTIR spectroscopy to detect DNA methylation in DNA-HALO samples more precisely compared to classical DNA extraction procedure that yield unstructured whole genomic DNA.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia",
title = "Methylation profile analysis of Of DNA-HALO structure by synchrotron-based FTIR spectroscopy",
pages = "68-68",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6304"
}

Sarić, A., Rajić, J., Tolić, A., Đorđević, M., Grdović, N., Uskoković, A., Dinić, S., Arambašić Jovanović, J., Mihailović, M., Vidaković, M.,& Dučić, T.. (2023). Methylation profile analysis of Of DNA-HALO structure by synchrotron-based FTIR spectroscopy. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade., 68-68.
https://hdl.handle.net/21.15107/rcub_ibiss_6304

Sarić A, Rajić J, Tolić A, Đorđević M, Grdović N, Uskoković A, Dinić S, Arambašić Jovanović J, Mihailović M, Vidaković M, Dučić T. Methylation profile analysis of Of DNA-HALO structure by synchrotron-based FTIR spectroscopy. in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia. 2023;:68-68.
https://hdl.handle.net/21.15107/rcub_ibiss_6304 .

Sarić, Ana, Rajić, Jovana, Tolić, Anja, Đorđević, Marija, Grdović, Nevena, Uskoković, Aleksandra, Dinić, Svetlana, Arambašić Jovanović, Jelena, Mihailović, Mirjana, Vidaković, Melita, Dučić, Tanja, "Methylation profile analysis of Of DNA-HALO structure by synchrotron-based FTIR spectroscopy" in Abstract Book: CoMBoS2 - the Second Congress of Molecular Biologists of Serbia; 2023 Oct 6-8; Belgrade, Serbia (2023):68-68,
https://hdl.handle.net/21.15107/rcub_ibiss_6304 .

TY  - JOUR
AU  - Sarić, Ana
AU  - Rajić, Jovana
AU  - Tolić, Anja
AU  - Dučić, Tanja
AU  - Vidaković, Melita
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5948
AB  - Fourier transform infrared (FTIR) spectroscopy is a rapid, non-destructive and label-free technique for identifying subtle changes in all bio-macromolecules, and has been used as a method of choice for studying DNA conformation, secondary DNA structure transition and DNA damage. In addition, the specific level of chromatin complexity is introduced via epigenetic modifications forcing the technological upgrade in the analysis of such an intricacy. As the most studied epigenetic mechanism, DNA methylation is a major regulator of transcriptional activity, involved in the suppression of a broad spectrum of genes and its deregulation is involved in all non-communicable diseases. The present study was designed to explore the use of synchrotron-based FTIR analysis to monitor the subtle changes in molecule bases regarding the DNA methylation status of cytosine in the whole genome. In order to reveal the conformation-related best sample for FTIR-based DNA methylation analysis in situ, we used methodology for nuclear HALO preparations and slightly modified it to isolated DNA in HALO formations. Nuclear DNA-HALOs represent samples with preserved higher-order chromatin structure liberated of any protein residues that are closer to native DNA conformation than genomic DNA (gDNA) isolated by the standard batch procedure. Using FTIR spectroscopy we analyzed the DNA methylation profile of isolated gDNA and compared it with the DNA-HALOs. This study demonstrated the potential of FTIR microspectroscopy to detect DNA methylation marks in analyzed DNA-HALO specimens more precisely in comparison with classical DNA extraction procedures that yield unstructured whole genomic DNA. In addition, we used different cell types to assess their global DNA methylation profile, as well as defined specific infrared peaks that can be used for screening DNA methylation.
PB  - Elsevier
T2  - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
T1  - Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure
IS  - 123090
VL  - 302
DO  - 10.1016/j.saa.2023.123090
ER  - 

@article{
author = "Sarić, Ana and Rajić, Jovana and Tolić, Anja and Dučić, Tanja and Vidaković, Melita",
year = "2023",
abstract = "Fourier transform infrared (FTIR) spectroscopy is a rapid, non-destructive and label-free technique for identifying subtle changes in all bio-macromolecules, and has been used as a method of choice for studying DNA conformation, secondary DNA structure transition and DNA damage. In addition, the specific level of chromatin complexity is introduced via epigenetic modifications forcing the technological upgrade in the analysis of such an intricacy. As the most studied epigenetic mechanism, DNA methylation is a major regulator of transcriptional activity, involved in the suppression of a broad spectrum of genes and its deregulation is involved in all non-communicable diseases. The present study was designed to explore the use of synchrotron-based FTIR analysis to monitor the subtle changes in molecule bases regarding the DNA methylation status of cytosine in the whole genome. In order to reveal the conformation-related best sample for FTIR-based DNA methylation analysis in situ, we used methodology for nuclear HALO preparations and slightly modified it to isolated DNA in HALO formations. Nuclear DNA-HALOs represent samples with preserved higher-order chromatin structure liberated of any protein residues that are closer to native DNA conformation than genomic DNA (gDNA) isolated by the standard batch procedure. Using FTIR spectroscopy we analyzed the DNA methylation profile of isolated gDNA and compared it with the DNA-HALOs. This study demonstrated the potential of FTIR microspectroscopy to detect DNA methylation marks in analyzed DNA-HALO specimens more precisely in comparison with classical DNA extraction procedures that yield unstructured whole genomic DNA. In addition, we used different cell types to assess their global DNA methylation profile, as well as defined specific infrared peaks that can be used for screening DNA methylation.",
publisher = "Elsevier",
journal = "Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy",
title = "Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure",
number = "123090",
volume = "302",
doi = "10.1016/j.saa.2023.123090"
}

Sarić, A., Rajić, J., Tolić, A., Dučić, T.,& Vidaković, M.. (2023). Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure. in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Elsevier., 302(123090).
https://doi.org/10.1016/j.saa.2023.123090

Sarić A, Rajić J, Tolić A, Dučić T, Vidaković M. Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure. in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2023;302(123090).
doi:10.1016/j.saa.2023.123090 .

Sarić, Ana, Rajić, Jovana, Tolić, Anja, Dučić, Tanja, Vidaković, Melita, "Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure" in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 302, no. 123090 (2023),
https://doi.org/10.1016/j.saa.2023.123090 . .

TY  - JOUR
AU  - Sarić, Ana
AU  - Rajić, Jovana
AU  - Tolić, Anja
AU  - Dučić, Tanja
AU  - Vidaković, Melita
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5947
AB  - Fourier transform infrared (FTIR) spectroscopy is a rapid, non-destructive and label-free technique for identifying subtle changes in all bio-macromolecules, and has been used as a method of choice for studying DNA conformation, secondary DNA structure transition and DNA damage. In addition, the specific level of chromatin complexity is introduced via epigenetic modifications forcing the technological upgrade in the analysis of such an intricacy. As the most studied epigenetic mechanism, DNA methylation is a major regulator of transcriptional activity, involved in the suppression of a broad spectrum of genes and its deregulation is involved in all non-communicable diseases. The present study was designed to explore the use of synchrotron-based FTIR analysis to monitor the subtle changes in molecule bases regarding the DNA methylation status of cytosine in the whole genome. In order to reveal the conformation-related best sample for FTIR-based DNA methylation analysis in situ, we used methodology for nuclear HALO preparations and slightly modified it to isolated DNA in HALO formations. Nuclear DNA-HALOs represent samples with preserved higher-order chromatin structure liberated of any protein residues that are closer to native DNA conformation than genomic DNA (gDNA) isolated by the standard batch procedure. Using FTIR spectroscopy we analyzed the DNA methylation profile of isolated gDNA and compared it with the DNA-HALOs. This study demonstrated the potential of FTIR microspectroscopy to detect DNA methylation marks in analyzed DNA-HALO specimens more precisely in comparison with classical DNA extraction procedures that yield unstructured whole genomic DNA. In addition, we used different cell types to assess their global DNA methylation profile, as well as defined specific infrared peaks that can be used for screening DNA methylation.
PB  - Elsevier
T2  - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
T1  - Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure
IS  - 123090
VL  - 302
DO  - 10.1016/j.saa.2023.123090
ER  - 

@article{
author = "Sarić, Ana and Rajić, Jovana and Tolić, Anja and Dučić, Tanja and Vidaković, Melita",
year = "2023",
abstract = "Fourier transform infrared (FTIR) spectroscopy is a rapid, non-destructive and label-free technique for identifying subtle changes in all bio-macromolecules, and has been used as a method of choice for studying DNA conformation, secondary DNA structure transition and DNA damage. In addition, the specific level of chromatin complexity is introduced via epigenetic modifications forcing the technological upgrade in the analysis of such an intricacy. As the most studied epigenetic mechanism, DNA methylation is a major regulator of transcriptional activity, involved in the suppression of a broad spectrum of genes and its deregulation is involved in all non-communicable diseases. The present study was designed to explore the use of synchrotron-based FTIR analysis to monitor the subtle changes in molecule bases regarding the DNA methylation status of cytosine in the whole genome. In order to reveal the conformation-related best sample for FTIR-based DNA methylation analysis in situ, we used methodology for nuclear HALO preparations and slightly modified it to isolated DNA in HALO formations. Nuclear DNA-HALOs represent samples with preserved higher-order chromatin structure liberated of any protein residues that are closer to native DNA conformation than genomic DNA (gDNA) isolated by the standard batch procedure. Using FTIR spectroscopy we analyzed the DNA methylation profile of isolated gDNA and compared it with the DNA-HALOs. This study demonstrated the potential of FTIR microspectroscopy to detect DNA methylation marks in analyzed DNA-HALO specimens more precisely in comparison with classical DNA extraction procedures that yield unstructured whole genomic DNA. In addition, we used different cell types to assess their global DNA methylation profile, as well as defined specific infrared peaks that can be used for screening DNA methylation.",
publisher = "Elsevier",
journal = "Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy",
title = "Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure",
number = "123090",
volume = "302",
doi = "10.1016/j.saa.2023.123090"
}

Sarić, A., Rajić, J., Tolić, A., Dučić, T.,& Vidaković, M.. (2023). Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure. in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Elsevier., 302(123090).
https://doi.org/10.1016/j.saa.2023.123090

Sarić A, Rajić J, Tolić A, Dučić T, Vidaković M. Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure. in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2023;302(123090).
doi:10.1016/j.saa.2023.123090 .

Sarić, Ana, Rajić, Jovana, Tolić, Anja, Dučić, Tanja, Vidaković, Melita, "Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure" in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 302, no. 123090 (2023),
https://doi.org/10.1016/j.saa.2023.123090 . .

TY  - DATA
AU  - Sarić, Ana
AU  - Rajić, Jovana
AU  - Tolić, Anja
AU  - Dučić, Tanja
AU  - Vidaković, Melita
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5960
AB  - Supplementary Figure 1:
Curve fitting of FTIR spectra for analyzing differences in DNA regions.
PB  - Elsevier
T2  - Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
T1  - Supplementary material for the article: Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure
IS  - 123090
VL  - 302
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5960
ER  - 

@misc{
author = "Sarić, Ana and Rajić, Jovana and Tolić, Anja and Dučić, Tanja and Vidaković, Melita",
year = "2023",
abstract = "Supplementary Figure 1:
Curve fitting of FTIR spectra for analyzing differences in DNA regions.",
publisher = "Elsevier",
journal = "Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy",
title = "Supplementary material for the article: Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure",
number = "123090",
volume = "302",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5960"
}

Sarić, A., Rajić, J., Tolić, A., Dučić, T.,& Vidaković, M.. (2023). Supplementary material for the article: Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure. in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
Elsevier., 302(123090).
https://hdl.handle.net/21.15107/rcub_ibiss_5960

Sarić A, Rajić J, Tolić A, Dučić T, Vidaković M. Supplementary material for the article: Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure. in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2023;302(123090).
https://hdl.handle.net/21.15107/rcub_ibiss_5960 .

Sarić, Ana, Rajić, Jovana, Tolić, Anja, Dučić, Tanja, Vidaković, Melita, "Supplementary material for the article: Synchrotron-based FTIR microspectroscopy reveals DNA methylation profile in DNA-HALO structure" in Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 302, no. 123090 (2023),
https://hdl.handle.net/21.15107/rcub_ibiss_5960 .

TY  - CONF
AU  - Sarić, Ana
AU  - Tolić, Anja
AU  - Grdović, Nevena
AU  - Dinić, Svetlana
AU  - Rajić, Jovana
AU  - Đorđević, Miloš
AU  - Đorđević, Marija
AU  - Arambašić Jovanović, Jelena
AU  - Mihailović, Mirjana
AU  - Uskoković, Aleksandra
AU  - Dučić, Tanja
AU  - Vidaković, Melita
PY  - 2019
UR  - https://www.cost.eu/cost-events/the-extracellular-vesicles-paradigm-of-intra-intercellular-communication/
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5920
AB  - Double-strand deoxyribonucleic acid (dsDNA) carries the genetic information needed for normal development, growth, survival and reproduction of all living beings (except RNA viruses and other potential DNA-less microorganisms). On top of it, epigenetic processes orchestrate the cell type-specific use of the genetic information essential for normal development and for maintaining the overall integrity of the genome. The alteration of epigenetic marks (e.g. DNA methylation patterns) by hyperglycaemia, oxidative stress and inflammation may have potential epigenetic impacts on gene regulation in diabetic individuals. We used the Fourier transform-infrared (FTIR) spectroscopy (ALBA synchrotron, Cerdanyola del valles, Spain) for qualitative spectral analysis of DNA from mouse fibroblast cells (NIH3T3) and the same cells treated with demetilating agent 5-azacytidine (5-aza). FTIR spectroscopy has the advantage of generating structural information of the entire DNA molecule in a single spectrum, including possible conformational sub-states, present in the sample (methylated/nonmethylated cytosine). The technique is ideal for systematic studies of DNA/RNA sequence variations and covalent modifications, since it is non-destructive and requires only small sample amounts. The FTIR region of interest when studying nucleic acids is 1800–900 cm-1.We obtained the global information regarding the DNA profiles in NIH3T3 with and without 5-aza treatment by FTIR spectroscopy. Some differences in DNA methylation profiles between examined cell lines were qualitatively described by FTIR spectroscopy and compared with restriction analysis method. Using FTIR spectroscopy the most interesting picks were observed approximately at wavelength: 2960-2850 cm-1, 1400-900 cm-1 and 1150 cm-1. These results are in the same time a verification of the proof of principle for synchrotron-based FTIR micro-spectroscopic detection of the differences in the DNA methylation profiles in cells.
PB  - COST Action CellFit
C3  - 3rd CellFit annual meeting: The extracellular vesicles paradigm of intracellular communication; 2019 Oct 10-12; Athens, Greece
T1  - The FTIR spectroscopy as a method of choice for detecting changes in DNA profiles of the mouse embryonic fibroblasts after the treatment with 5-aza
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5920
ER  - 

@conference{
author = "Sarić, Ana and Tolić, Anja and Grdović, Nevena and Dinić, Svetlana and Rajić, Jovana and Đorđević, Miloš and Đorđević, Marija and Arambašić Jovanović, Jelena and Mihailović, Mirjana and Uskoković, Aleksandra and Dučić, Tanja and Vidaković, Melita",
year = "2019",
abstract = "Double-strand deoxyribonucleic acid (dsDNA) carries the genetic information needed for normal development, growth, survival and reproduction of all living beings (except RNA viruses and other potential DNA-less microorganisms). On top of it, epigenetic processes orchestrate the cell type-specific use of the genetic information essential for normal development and for maintaining the overall integrity of the genome. The alteration of epigenetic marks (e.g. DNA methylation patterns) by hyperglycaemia, oxidative stress and inflammation may have potential epigenetic impacts on gene regulation in diabetic individuals. We used the Fourier transform-infrared (FTIR) spectroscopy (ALBA synchrotron, Cerdanyola del valles, Spain) for qualitative spectral analysis of DNA from mouse fibroblast cells (NIH3T3) and the same cells treated with demetilating agent 5-azacytidine (5-aza). FTIR spectroscopy has the advantage of generating structural information of the entire DNA molecule in a single spectrum, including possible conformational sub-states, present in the sample (methylated/nonmethylated cytosine). The technique is ideal for systematic studies of DNA/RNA sequence variations and covalent modifications, since it is non-destructive and requires only small sample amounts. The FTIR region of interest when studying nucleic acids is 1800–900 cm-1.We obtained the global information regarding the DNA profiles in NIH3T3 with and without 5-aza treatment by FTIR spectroscopy. Some differences in DNA methylation profiles between examined cell lines were qualitatively described by FTIR spectroscopy and compared with restriction analysis method. Using FTIR spectroscopy the most interesting picks were observed approximately at wavelength: 2960-2850 cm-1, 1400-900 cm-1 and 1150 cm-1. These results are in the same time a verification of the proof of principle for synchrotron-based FTIR micro-spectroscopic detection of the differences in the DNA methylation profiles in cells.",
publisher = "COST Action CellFit",
journal = "3rd CellFit annual meeting: The extracellular vesicles paradigm of intracellular communication; 2019 Oct 10-12; Athens, Greece",
title = "The FTIR spectroscopy as a method of choice for detecting changes in DNA profiles of the mouse embryonic fibroblasts after the treatment with 5-aza",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5920"
}

Sarić, A., Tolić, A., Grdović, N., Dinić, S., Rajić, J., Đorđević, M., Đorđević, M., Arambašić Jovanović, J., Mihailović, M., Uskoković, A., Dučić, T.,& Vidaković, M.. (2019). The FTIR spectroscopy as a method of choice for detecting changes in DNA profiles of the mouse embryonic fibroblasts after the treatment with 5-aza. in 3rd CellFit annual meeting: The extracellular vesicles paradigm of intracellular communication; 2019 Oct 10-12; Athens, Greece
COST Action CellFit..
https://hdl.handle.net/21.15107/rcub_ibiss_5920

Sarić A, Tolić A, Grdović N, Dinić S, Rajić J, Đorđević M, Đorđević M, Arambašić Jovanović J, Mihailović M, Uskoković A, Dučić T, Vidaković M. The FTIR spectroscopy as a method of choice for detecting changes in DNA profiles of the mouse embryonic fibroblasts after the treatment with 5-aza. in 3rd CellFit annual meeting: The extracellular vesicles paradigm of intracellular communication; 2019 Oct 10-12; Athens, Greece. 2019;.
https://hdl.handle.net/21.15107/rcub_ibiss_5920 .

Sarić, Ana, Tolić, Anja, Grdović, Nevena, Dinić, Svetlana, Rajić, Jovana, Đorđević, Miloš, Đorđević, Marija, Arambašić Jovanović, Jelena, Mihailović, Mirjana, Uskoković, Aleksandra, Dučić, Tanja, Vidaković, Melita, "The FTIR spectroscopy as a method of choice for detecting changes in DNA profiles of the mouse embryonic fibroblasts after the treatment with 5-aza" in 3rd CellFit annual meeting: The extracellular vesicles paradigm of intracellular communication; 2019 Oct 10-12; Athens, Greece (2019),
https://hdl.handle.net/21.15107/rcub_ibiss_5920 .

TY  - CONF
AU  - Sarić, Ana
AU  - Tolić, Anja
AU  - Grdović, Nevena
AU  - Dinić, Svetlana
AU  - Rajić, Jovana
AU  - Đorđević, Miloš
AU  - Đorđević, Marija
AU  - Arambašić Jovanović, Jelena
AU  - Mihailović, Mirjana
AU  - Poznanović, Goran
AU  - Uskoković, Aleksandra
AU  - Vidaković, Melita
AU  - Dučić, Tanja
PY  - 2019
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5694
AB  - Epigenetic processes orchestrate the cell type-specific use of the genetic information
essential for normal development and for maintaining the overall integrity of the genome.
DNA methylation is probably the most extensively studied epigenetic mark and represents
the covalent attachment of a methyl group to cytosine that is located next to guanine within
the genomic DNA. The alteration of DNA methylation patterns by hyperglycaemia, oxidative
stress and inflammation may have potential epigenetic impacts on gene regulation in diabetic
individuals. Further research devoted to improve the steps that could be undertaken in the
early diagnosis, prevention and treatment of diabetes and its complications is a scientifically
and socially significant task. We used the Fourier transform-infrared (FTIR) spectroscopy
(ALBA synchrotron, Cerdanyola del valles, Spain) for qualitative spectral analysis of
normomethylated DNA from mouse fibroblast cells (NIH3T3) and hypomethylated DNA
from PARP-1 knockout mouse fibroblast cells (PARP-/-). We obtained the global information
regarding the DNA methylation profiles in mouse fibroblast cells by FTIR spectroscopy that
was visualised by immune-fluorescent staining using anti-methyl cytosine (5mC) antibody.
Some differences in DNA methylation profiles between examined cell lines were observed
in spectral region significant for cytosine (990-1250 nm). The most interesting picks were
observed approximately at wavelength: 1240 nm, 1150 nm, 1110 nm and 1010 nm. These
results are in the same time a verification of the proof of principle for FTIR based analysis of
the differences between normomethylated and hypomethylated genomic DNA which could
be set as a potential predictive and diagnostic tool in future. To our knowledge this is a first
time that synchrotron-based FTIR micro-spectroscopy is used for detection of the presence
of 5mC and changes in the DNA methylation profile in cells.
PB  - Belgrade: Serbian Genetic Society
C3  - 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
T1  - Detection of DNA methylation profiles in mouse embryonic fibroblasts using Fourier transform-infrared spectroscopy
SP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_5694
ER  - 

@conference{
author = "Sarić, Ana and Tolić, Anja and Grdović, Nevena and Dinić, Svetlana and Rajić, Jovana and Đorđević, Miloš and Đorđević, Marija and Arambašić Jovanović, Jelena and Mihailović, Mirjana and Poznanović, Goran and Uskoković, Aleksandra and Vidaković, Melita and Dučić, Tanja",
year = "2019",
abstract = "Epigenetic processes orchestrate the cell type-specific use of the genetic information
essential for normal development and for maintaining the overall integrity of the genome.
DNA methylation is probably the most extensively studied epigenetic mark and represents
the covalent attachment of a methyl group to cytosine that is located next to guanine within
the genomic DNA. The alteration of DNA methylation patterns by hyperglycaemia, oxidative
stress and inflammation may have potential epigenetic impacts on gene regulation in diabetic
individuals. Further research devoted to improve the steps that could be undertaken in the
early diagnosis, prevention and treatment of diabetes and its complications is a scientifically
and socially significant task. We used the Fourier transform-infrared (FTIR) spectroscopy
(ALBA synchrotron, Cerdanyola del valles, Spain) for qualitative spectral analysis of
normomethylated DNA from mouse fibroblast cells (NIH3T3) and hypomethylated DNA
from PARP-1 knockout mouse fibroblast cells (PARP-/-). We obtained the global information
regarding the DNA methylation profiles in mouse fibroblast cells by FTIR spectroscopy that
was visualised by immune-fluorescent staining using anti-methyl cytosine (5mC) antibody.
Some differences in DNA methylation profiles between examined cell lines were observed
in spectral region significant for cytosine (990-1250 nm). The most interesting picks were
observed approximately at wavelength: 1240 nm, 1150 nm, 1110 nm and 1010 nm. These
results are in the same time a verification of the proof of principle for FTIR based analysis of
the differences between normomethylated and hypomethylated genomic DNA which could
be set as a potential predictive and diagnostic tool in future. To our knowledge this is a first
time that synchrotron-based FTIR micro-spectroscopy is used for detection of the presence
of 5mC and changes in the DNA methylation profile in cells.",
publisher = "Belgrade: Serbian Genetic Society",
journal = "6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia",
title = "Detection of DNA methylation profiles in mouse embryonic fibroblasts using Fourier transform-infrared spectroscopy",
pages = "75",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_5694"
}

Sarić, A., Tolić, A., Grdović, N., Dinić, S., Rajić, J., Đorđević, M., Đorđević, M., Arambašić Jovanović, J., Mihailović, M., Poznanović, G., Uskoković, A., Vidaković, M.,& Dučić, T.. (2019). Detection of DNA methylation profiles in mouse embryonic fibroblasts using Fourier transform-infrared spectroscopy. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia
Belgrade: Serbian Genetic Society., 75.
https://hdl.handle.net/21.15107/rcub_ibiss_5694

Sarić A, Tolić A, Grdović N, Dinić S, Rajić J, Đorđević M, Đorđević M, Arambašić Jovanović J, Mihailović M, Poznanović G, Uskoković A, Vidaković M, Dučić T. Detection of DNA methylation profiles in mouse embryonic fibroblasts using Fourier transform-infrared spectroscopy. in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia. 2019;:75.
https://hdl.handle.net/21.15107/rcub_ibiss_5694 .

Sarić, Ana, Tolić, Anja, Grdović, Nevena, Dinić, Svetlana, Rajić, Jovana, Đorđević, Miloš, Đorđević, Marija, Arambašić Jovanović, Jelena, Mihailović, Mirjana, Poznanović, Goran, Uskoković, Aleksandra, Vidaković, Melita, Dučić, Tanja, "Detection of DNA methylation profiles in mouse embryonic fibroblasts using Fourier transform-infrared spectroscopy" in 6th Congress of the Serbian genetic society: Book of abstracts; 2019 Oct 13-17; Vrnjačka Banja, Serbia (2019):75,
https://hdl.handle.net/21.15107/rcub_ibiss_5694 .

TY  - JOUR
AU  - Radotić, Ksenija
AU  - Dučić, Tanja M.
AU  - Prodanović, Radivoje M.
AU  - Vujčić, Zoran M.
AU  - Karadžić, Branko
AU  - Antić-Jovanović, Svetlana
PY  - 2003
PY  - 2003
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/27
AB  - We studied the soluble peroxidase and superoxid-dismutase activity peroxidase isoenzyme pattern and metal content in the needles of 2-year-old spruce grown on soils supplemented with cadmium and nickel concentrations from 3 to 30 mg kg -1 and 50 to 500 mg kg -1 respectively. The two metals were applied both separately and simultaneously. The kinetics of metal assimilation and total accumulated quantity depended on the type of treatment. Following metal exposure, an increase in peroxidase activity in the seedlings treated with Cd and Ni/Cd, and in superoxide-dismutase activity during Ni/Cd treatment was observed. A parallel change of peroxidase isoenzyme pattern occurred. There was a good correlation between accumulated metals in needles and enzyme activities. The effect of Cd on the measured parameters was shown to be stronger comparing to the effect of Ni. The results obtained show that peroxidase and superoxide-dismutase activity and peroxidase isoenzyme pattern could be used to evaluate the capacity of one part of the defense system in spruce seedlings to with-stand metal stress.
AB  - Proučavana je aktivnost slobodnih peroksidaza i superoksid-dismutaza izoenzimski profil peroksidaza i sadržaj metala u četinama dvogodišnjih smrča gajenih na zemljištu u koje su dodavani kadmijum i nikl u koncentracijama 3 do 30 mg kg -1 i 50 do 500 mg kg -1 respektivno. Metali su dodavani na dva načina: zasebno i istovremeno. Kinetika usvajanja metala i ukupna akumulirana količina zavisili su od vrste tretmana. U tretiranim izdancima došlo je do povećanja peroksidazne aktivnosti u slučaju tretmana kadmijumom i nikl/kadmijumom, kao i superoksid-dismutazne aktivnosti u toku tretmana nikl/kadmijumom, u odnosu na netretirane biljke. Istovremeno je došlo do promene izoenzimskog sastava peroksidaza. Koncentracija akumuliranih metala u četinama je bila u dobroj korelaciji sa enzimskim aktivnostima. Pokazano je da je uticaj kadmijuma na merene parametre jači u poređenju sa uticajem nikla. Dobijeni rezultati pokazuju da peroksidazna i superoksid-dismutazna aktivnost i izoenzimski profil peroksidaza mogu da se koriste za procenu kapaciteta jednog dela zaštitnog sistema u izdancima smrče u odnosu na stres izazvan metalima.
T2  - Jugoslovenska medicinska biohemija
T1  - Toksičnost nikla i kadmijuma u četinama smrče: uticaj zasebnih i kombinovanih tretmana na peroksidaznu i superoksid-dismutaznu aktivnost
T1  - Toxicity of nickel and cadmium in spruce seedlings: Effect of separated and combined treatments on peroxidase and superoxide-dismutase activity
IS  - 1
VL  - 22
SP  - 41
EP  - 52
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_27
ER  - 

@article{
author = "Radotić, Ksenija and Dučić, Tanja M. and Prodanović, Radivoje M. and Vujčić, Zoran M. and Karadžić, Branko and Antić-Jovanović, Svetlana",
year = "2003, 2003",
abstract = "We studied the soluble peroxidase and superoxid-dismutase activity peroxidase isoenzyme pattern and metal content in the needles of 2-year-old spruce grown on soils supplemented with cadmium and nickel concentrations from 3 to 30 mg kg -1 and 50 to 500 mg kg -1 respectively. The two metals were applied both separately and simultaneously. The kinetics of metal assimilation and total accumulated quantity depended on the type of treatment. Following metal exposure, an increase in peroxidase activity in the seedlings treated with Cd and Ni/Cd, and in superoxide-dismutase activity during Ni/Cd treatment was observed. A parallel change of peroxidase isoenzyme pattern occurred. There was a good correlation between accumulated metals in needles and enzyme activities. The effect of Cd on the measured parameters was shown to be stronger comparing to the effect of Ni. The results obtained show that peroxidase and superoxide-dismutase activity and peroxidase isoenzyme pattern could be used to evaluate the capacity of one part of the defense system in spruce seedlings to with-stand metal stress., Proučavana je aktivnost slobodnih peroksidaza i superoksid-dismutaza izoenzimski profil peroksidaza i sadržaj metala u četinama dvogodišnjih smrča gajenih na zemljištu u koje su dodavani kadmijum i nikl u koncentracijama 3 do 30 mg kg -1 i 50 do 500 mg kg -1 respektivno. Metali su dodavani na dva načina: zasebno i istovremeno. Kinetika usvajanja metala i ukupna akumulirana količina zavisili su od vrste tretmana. U tretiranim izdancima došlo je do povećanja peroksidazne aktivnosti u slučaju tretmana kadmijumom i nikl/kadmijumom, kao i superoksid-dismutazne aktivnosti u toku tretmana nikl/kadmijumom, u odnosu na netretirane biljke. Istovremeno je došlo do promene izoenzimskog sastava peroksidaza. Koncentracija akumuliranih metala u četinama je bila u dobroj korelaciji sa enzimskim aktivnostima. Pokazano je da je uticaj kadmijuma na merene parametre jači u poređenju sa uticajem nikla. Dobijeni rezultati pokazuju da peroksidazna i superoksid-dismutazna aktivnost i izoenzimski profil peroksidaza mogu da se koriste za procenu kapaciteta jednog dela zaštitnog sistema u izdancima smrče u odnosu na stres izazvan metalima.",
journal = "Jugoslovenska medicinska biohemija",
title = "Toksičnost nikla i kadmijuma u četinama smrče: uticaj zasebnih i kombinovanih tretmana na peroksidaznu i superoksid-dismutaznu aktivnost, Toxicity of nickel and cadmium in spruce seedlings: Effect of separated and combined treatments on peroxidase and superoxide-dismutase activity",
number = "1",
volume = "22",
pages = "41-52",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_27"
}

Radotić, K., Dučić, T. M., Prodanović, R. M., Vujčić, Z. M., Karadžić, B.,& Antić-Jovanović, S.. (2003). Toksičnost nikla i kadmijuma u četinama smrče: uticaj zasebnih i kombinovanih tretmana na peroksidaznu i superoksid-dismutaznu aktivnost. in Jugoslovenska medicinska biohemija, 22(1), 41-52.
https://hdl.handle.net/21.15107/rcub_ibiss_27

Radotić K, Dučić TM, Prodanović RM, Vujčić ZM, Karadžić B, Antić-Jovanović S. Toksičnost nikla i kadmijuma u četinama smrče: uticaj zasebnih i kombinovanih tretmana na peroksidaznu i superoksid-dismutaznu aktivnost. in Jugoslovenska medicinska biohemija. 2003;22(1):41-52.
https://hdl.handle.net/21.15107/rcub_ibiss_27 .

Radotić, Ksenija, Dučić, Tanja M., Prodanović, Radivoje M., Vujčić, Zoran M., Karadžić, Branko, Antić-Jovanović, Svetlana, "Toksičnost nikla i kadmijuma u četinama smrče: uticaj zasebnih i kombinovanih tretmana na peroksidaznu i superoksid-dismutaznu aktivnost" in Jugoslovenska medicinska biohemija, 22, no. 1 (2003):41-52,
https://hdl.handle.net/21.15107/rcub_ibiss_27 .