TY  - CHAP
AU  - Dobrijević, Zorana
AU  - Matijašević-Joković, Suzana
AU  - Branković, Ana
AU  - Đorđević, Ana
AU  - Popović, Milica
AU  - Brajušković, Goran
PY  - 2022
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6565
AB  - U ovom radu preglednog tipa prikazano je desetogodišnje iskustvo istraživačkog tima PRO-
STATSERBIA koji za temu svog istraživanja ima molekularnu osnovu karcinoma prostate (KP). Centar
za humanu molekularnu genetiku Biološkog fakulteta Univerziteta u Beogradu poseduje kolekciju
uzoraka i banku podataka za gotovo 1000 muškaraca sa bolestima prostate (KP i benigna hiperpla-
zija prostate) i preko 350 muškaraca bez znakova bolesti prostate iz populacije Republike Srbije. Naj-
veći broj studija bio je dizajniran kao studije asocijacije odabranih genetičkih varijanti sa rizikom za
razvoj i progresiju KP. U ovim studijama slučajeva i kontola ispitivane su genetičke varijente kako u ge-
nima za proteine i nekodirajuće molekule RNK, tako i u nekodirajućim regionima genoma (tzv. „gen-
skim pustinjama“). Pored studija slučajeva i kontrola, sprovođene su i meta-analize kao i analize
statističkih epistatičkih interakcija analiziranih genetičkih varijanti. Krajnji cilj ovih studija je kreiranje
algoritma za procenu rizika za progresiju bolesti koji bi se koristio u fazi aktivnog praćenja bolesnika
sa ranodijagnostikovanim KP. U poslednje vreme, istraživanja su usmerena na egzozome i njihov ma-
kromolekulski sadržaj (proteine i nekodirajuće molekule RNK) kao potencijalne biološke markere
tečne biopsije KP. Pored toga, biološki makromolekuli na površini egzozoma predstavljaju i ciljane
molekule u novim strategijama lečenja KP.
AB  - This review-type paper will present the ten-year experience of the PROSTATSERBIA research team,
which has the molecular basis of prostate cancer (PCa) as its research topic. The Center for Human Mo-
lecular Genetics of the Faculty of Biology, University of Belgrade has a collection of samples and a
data bank for almost 1000 men with prostate diseases (PCa and benign prostatic hyperplasia) and for
over 350 men without signs of prostate diseases, all from Serbian population. Most of the studies
were designed as studies of association of selected genetic variants with the risk for development
and progression of PCa. In these case-control studies, genetic variants were examined both in genes
for proteins and non-coding RNAs, as well as in non-coding regions of the genome (so-called gene
deserts). In addition to case control studies, both meta-analyses and the analysis of statistical epista-
tic interactions of the analyzed genetic variants were conducted. The main goal of all these studies was
to create an algorithm for risk assessment for disease progression that would be used in the phase of
active monitoring of patients with early diagnosed PCa. Recently, we shifted our research focus on
exosomes and their macromolecular content (proteins and microRNAs) as potential biological mar-
kers of liquid prostate cancer biopsy. In addition, macromolecules at the exosome surface represent
target molecules for new PCa treatment strategies.
PB  - Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade
T2  - Trends in molecular biology
T1  - Savremeni pristupi u istraživanju molekularne osnove karcinoma prostate
T1  - Modern approaches in research of the molecular basis of prostate cancer
IS  - 2
SP  - 63
EP  - 74
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6565
ER  - 

@inbook{
author = "Dobrijević, Zorana and Matijašević-Joković, Suzana and Branković, Ana and Đorđević, Ana and Popović, Milica and Brajušković, Goran",
year = "2022",
abstract = "U ovom radu preglednog tipa prikazano je desetogodišnje iskustvo istraživačkog tima PRO-
STATSERBIA koji za temu svog istraživanja ima molekularnu osnovu karcinoma prostate (KP). Centar
za humanu molekularnu genetiku Biološkog fakulteta Univerziteta u Beogradu poseduje kolekciju
uzoraka i banku podataka za gotovo 1000 muškaraca sa bolestima prostate (KP i benigna hiperpla-
zija prostate) i preko 350 muškaraca bez znakova bolesti prostate iz populacije Republike Srbije. Naj-
veći broj studija bio je dizajniran kao studije asocijacije odabranih genetičkih varijanti sa rizikom za
razvoj i progresiju KP. U ovim studijama slučajeva i kontola ispitivane su genetičke varijente kako u ge-
nima za proteine i nekodirajuće molekule RNK, tako i u nekodirajućim regionima genoma (tzv. „gen-
skim pustinjama“). Pored studija slučajeva i kontrola, sprovođene su i meta-analize kao i analize
statističkih epistatičkih interakcija analiziranih genetičkih varijanti. Krajnji cilj ovih studija je kreiranje
algoritma za procenu rizika za progresiju bolesti koji bi se koristio u fazi aktivnog praćenja bolesnika
sa ranodijagnostikovanim KP. U poslednje vreme, istraživanja su usmerena na egzozome i njihov ma-
kromolekulski sadržaj (proteine i nekodirajuće molekule RNK) kao potencijalne biološke markere
tečne biopsije KP. Pored toga, biološki makromolekuli na površini egzozoma predstavljaju i ciljane
molekule u novim strategijama lečenja KP., This review-type paper will present the ten-year experience of the PROSTATSERBIA research team,
which has the molecular basis of prostate cancer (PCa) as its research topic. The Center for Human Mo-
lecular Genetics of the Faculty of Biology, University of Belgrade has a collection of samples and a
data bank for almost 1000 men with prostate diseases (PCa and benign prostatic hyperplasia) and for
over 350 men without signs of prostate diseases, all from Serbian population. Most of the studies
were designed as studies of association of selected genetic variants with the risk for development
and progression of PCa. In these case-control studies, genetic variants were examined both in genes
for proteins and non-coding RNAs, as well as in non-coding regions of the genome (so-called gene
deserts). In addition to case control studies, both meta-analyses and the analysis of statistical epista-
tic interactions of the analyzed genetic variants were conducted. The main goal of all these studies was
to create an algorithm for risk assessment for disease progression that would be used in the phase of
active monitoring of patients with early diagnosed PCa. Recently, we shifted our research focus on
exosomes and their macromolecular content (proteins and microRNAs) as potential biological mar-
kers of liquid prostate cancer biopsy. In addition, macromolecules at the exosome surface represent
target molecules for new PCa treatment strategies.",
publisher = "Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade",
journal = "Trends in molecular biology",
booktitle = "Savremeni pristupi u istraživanju molekularne osnove karcinoma prostate, Modern approaches in research of the molecular basis of prostate cancer",
number = "2",
pages = "63-74",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6565"
}

Dobrijević, Z., Matijašević-Joković, S., Branković, A., Đorđević, A., Popović, M.,& Brajušković, G.. (2022). Savremeni pristupi u istraživanju molekularne osnove karcinoma prostate. in Trends in molecular biology
Belgrade: Institute of Molecular Genetics and Genetic Engineering, University of Belgrade.(2), 63-74.
https://hdl.handle.net/21.15107/rcub_ibiss_6565

Dobrijević Z, Matijašević-Joković S, Branković A, Đorđević A, Popović M, Brajušković G. Savremeni pristupi u istraživanju molekularne osnove karcinoma prostate. in Trends in molecular biology. 2022;(2):63-74.
https://hdl.handle.net/21.15107/rcub_ibiss_6565 .

Dobrijević, Zorana, Matijašević-Joković, Suzana, Branković, Ana, Đorđević, Ana, Popović, Milica, Brajušković, Goran, "Savremeni pristupi u istraživanju molekularne osnove karcinoma prostate" in Trends in molecular biology, no. 2 (2022):63-74,
https://hdl.handle.net/21.15107/rcub_ibiss_6565 .

TY  - JOUR
AU  - Brkušanin, Milos
AU  - Jeftović-Velkova, Irena
AU  - Jovanović, Vladimir
AU  - Perić, Stojan
AU  - Pešović, Jovan
AU  - Brajušković, Goran
AU  - Stević, Zorica
AU  - Savić-Pavićević, Dušanka
PY  - 2018
UR  - http://www.doiserbia.nb.rs/Article.aspx?ID=0370-81791800069B
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3452
AB  - Introduction/Objective. Amyotrophic lateral sclerosis (ALS) is a devastating motor neuron disease. The majority of cases are apparently sporadic ALS (SALS) with variants in susceptibility genes or sometimes in high-risk ALS genes. Two ALS susceptibility genes are SMN1, whose functional loss causes spinal muscular atrophy (SMA), and a nearly identical SMN2 gene, which modulates SMA severity. In this study we examined the association of copy number variations (CNVs) of SMN1 and SMN2 genes and two additional genes, SERF1 and NAIP, residing in the same genomic region (i.e. 5q13.2 segmental duplication), with SALS in patients from Serbia. Methods. Multiplex ligation-dependent probe amplification was used to determine CNVs of each gene in a clinically well-characterised group of 153 Serbian SALS patients and 153 controls. Results. Individual association between SMN1, SMN2, SERF1 or NAIP CNVs and SALS susceptibility or survival was not found. Survival curves based on the multivariable Cox regression analysis showed that three SMN1 copies, lower ALS Functional Rating Scale Revised (ALSFRS-R) score at the time of diagnosis, faster decline of the ALSFRS-R score over time, and shorter diagnostic delay result in shorter survival of Serbian SALS patients. Conclusion. Clinical variables might be complemented with the SMN1 copy number to improve prediction of survival in Serbian SALS patients.
T2  - Srpski arhiv za celokupno lekarstvo
T1  - SMN1 copy number as a modifying factor of survival in Serbian patients with sporadic amyotrophic lateral sclerosis
IS  - 11-12
VL  - 146
DO  - 10.2298/SARH180801069B
SP  - 646
EP  - 652
ER  - 

@article{
author = "Brkušanin, Milos and Jeftović-Velkova, Irena and Jovanović, Vladimir and Perić, Stojan and Pešović, Jovan and Brajušković, Goran and Stević, Zorica and Savić-Pavićević, Dušanka",
year = "2018",
abstract = "Introduction/Objective. Amyotrophic lateral sclerosis (ALS) is a devastating motor neuron disease. The majority of cases are apparently sporadic ALS (SALS) with variants in susceptibility genes or sometimes in high-risk ALS genes. Two ALS susceptibility genes are SMN1, whose functional loss causes spinal muscular atrophy (SMA), and a nearly identical SMN2 gene, which modulates SMA severity. In this study we examined the association of copy number variations (CNVs) of SMN1 and SMN2 genes and two additional genes, SERF1 and NAIP, residing in the same genomic region (i.e. 5q13.2 segmental duplication), with SALS in patients from Serbia. Methods. Multiplex ligation-dependent probe amplification was used to determine CNVs of each gene in a clinically well-characterised group of 153 Serbian SALS patients and 153 controls. Results. Individual association between SMN1, SMN2, SERF1 or NAIP CNVs and SALS susceptibility or survival was not found. Survival curves based on the multivariable Cox regression analysis showed that three SMN1 copies, lower ALS Functional Rating Scale Revised (ALSFRS-R) score at the time of diagnosis, faster decline of the ALSFRS-R score over time, and shorter diagnostic delay result in shorter survival of Serbian SALS patients. Conclusion. Clinical variables might be complemented with the SMN1 copy number to improve prediction of survival in Serbian SALS patients.",
journal = "Srpski arhiv za celokupno lekarstvo",
title = "SMN1 copy number as a modifying factor of survival in Serbian patients with sporadic amyotrophic lateral sclerosis",
number = "11-12",
volume = "146",
doi = "10.2298/SARH180801069B",
pages = "646-652"
}

Brkušanin, M., Jeftović-Velkova, I., Jovanović, V., Perić, S., Pešović, J., Brajušković, G., Stević, Z.,& Savić-Pavićević, D.. (2018). SMN1 copy number as a modifying factor of survival in Serbian patients with sporadic amyotrophic lateral sclerosis. in Srpski arhiv za celokupno lekarstvo, 146(11-12), 646-652.
https://doi.org/10.2298/SARH180801069B

Brkušanin M, Jeftović-Velkova I, Jovanović V, Perić S, Pešović J, Brajušković G, Stević Z, Savić-Pavićević D. SMN1 copy number as a modifying factor of survival in Serbian patients with sporadic amyotrophic lateral sclerosis. in Srpski arhiv za celokupno lekarstvo. 2018;146(11-12):646-652.
doi:10.2298/SARH180801069B .

Brkušanin, Milos, Jeftović-Velkova, Irena, Jovanović, Vladimir, Perić, Stojan, Pešović, Jovan, Brajušković, Goran, Stević, Zorica, Savić-Pavićević, Dušanka, "SMN1 copy number as a modifying factor of survival in Serbian patients with sporadic amyotrophic lateral sclerosis" in Srpski arhiv za celokupno lekarstvo, 146, no. 11-12 (2018):646-652,
https://doi.org/10.2298/SARH180801069B . .

TY  - JOUR
AU  - Karanović, Jelena
AU  - Ivković, Maja
AU  - Jovanović, Vladimir
AU  - Šviković, Saša
AU  - Pantović-Stefanović, Maja
AU  - Brkušanin, Miloš
AU  - Damjanović, Aleksandar
AU  - Brajušković, Goran
AU  - Savić-Pavićević, Dušanka
PY  - 2017
UR  - http://link.springer.com/10.1007/s00702-017-1677-z
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2554
AB  - Suicidal behavior has been associated with a deficient serotonin neurotransmission which is likely a consequence of individual genetic architecture, exposure to environmental factors and interactions of those factors. We examined whether the interaction of child abuse, TPH2 (tryptophan hydroxylase 2) variant rs4290270, affecting alternative splicing and editing of TPH2 pre-mRNAs, and ADARB1 (adenosine deaminase acting on RNA B1) variants rs4819035 and rs9983925 may influence the risk for suicide attempt in psychiatric patients. TPH2 rs4290270 was genotyped in 165 suicide attempters and 188 suicide non-attempters diagnosed with major depressive disorder, bipolar disorder and schizophrenia. Genotyping data for ADARB1 variants were taken over from our previous study. Child abuse before the age of 18 years was assessed using the Early Trauma Inventory-Self Report. Generalized linear models and backward selection were applied to identify the main and interacting effects of environmental and genetic factors, including psychiatric diagnoses, patients’ gender and age as covariates. Childhood general traumas were independently associated with suicide attempt. Two-way interaction between TPH2 rs4290270 and general traumas revealed that TT homozygotes with a history of general traumas had an increased risk for suicide attempt. Three-way interaction of general traumas, TPH2 rs4290270 and ADARB1 rs4819035 indicated that the highest predisposition to suicide attempt was observed in individuals who experienced general traumas and were TT homozygote for rs4290270 and TT homozygote for rs4819035. Our findings suggest that the risk for suicide attempt in psychiatric patients exposed to an adverse childhood environment may depend on TPH2 and ADARB1 variants.
T2  - Journal of Neural Transmission
T1  - Effect of childhood general traumas on suicide attempt depends on TPH2 and ADARB1 variants in psychiatric patients
DO  - 10.1007/s00702-017-1677-z
ER  - 

@article{
author = "Karanović, Jelena and Ivković, Maja and Jovanović, Vladimir and Šviković, Saša and Pantović-Stefanović, Maja and Brkušanin, Miloš and Damjanović, Aleksandar and Brajušković, Goran and Savić-Pavićević, Dušanka",
year = "2017",
abstract = "Suicidal behavior has been associated with a deficient serotonin neurotransmission which is likely a consequence of individual genetic architecture, exposure to environmental factors and interactions of those factors. We examined whether the interaction of child abuse, TPH2 (tryptophan hydroxylase 2) variant rs4290270, affecting alternative splicing and editing of TPH2 pre-mRNAs, and ADARB1 (adenosine deaminase acting on RNA B1) variants rs4819035 and rs9983925 may influence the risk for suicide attempt in psychiatric patients. TPH2 rs4290270 was genotyped in 165 suicide attempters and 188 suicide non-attempters diagnosed with major depressive disorder, bipolar disorder and schizophrenia. Genotyping data for ADARB1 variants were taken over from our previous study. Child abuse before the age of 18 years was assessed using the Early Trauma Inventory-Self Report. Generalized linear models and backward selection were applied to identify the main and interacting effects of environmental and genetic factors, including psychiatric diagnoses, patients’ gender and age as covariates. Childhood general traumas were independently associated with suicide attempt. Two-way interaction between TPH2 rs4290270 and general traumas revealed that TT homozygotes with a history of general traumas had an increased risk for suicide attempt. Three-way interaction of general traumas, TPH2 rs4290270 and ADARB1 rs4819035 indicated that the highest predisposition to suicide attempt was observed in individuals who experienced general traumas and were TT homozygote for rs4290270 and TT homozygote for rs4819035. Our findings suggest that the risk for suicide attempt in psychiatric patients exposed to an adverse childhood environment may depend on TPH2 and ADARB1 variants.",
journal = "Journal of Neural Transmission",
title = "Effect of childhood general traumas on suicide attempt depends on TPH2 and ADARB1 variants in psychiatric patients",
doi = "10.1007/s00702-017-1677-z"
}

Karanović, J., Ivković, M., Jovanović, V., Šviković, S., Pantović-Stefanović, M., Brkušanin, M., Damjanović, A., Brajušković, G.,& Savić-Pavićević, D.. (2017). Effect of childhood general traumas on suicide attempt depends on TPH2 and ADARB1 variants in psychiatric patients. in Journal of Neural Transmission.
https://doi.org/10.1007/s00702-017-1677-z

Karanović J, Ivković M, Jovanović V, Šviković S, Pantović-Stefanović M, Brkušanin M, Damjanović A, Brajušković G, Savić-Pavićević D. Effect of childhood general traumas on suicide attempt depends on TPH2 and ADARB1 variants in psychiatric patients. in Journal of Neural Transmission. 2017;.
doi:10.1007/s00702-017-1677-z .

Karanović, Jelena, Ivković, Maja, Jovanović, Vladimir, Šviković, Saša, Pantović-Stefanović, Maja, Brkušanin, Miloš, Damjanović, Aleksandar, Brajušković, Goran, Savić-Pavićević, Dušanka, "Effect of childhood general traumas on suicide attempt depends on TPH2 and ADARB1 variants in psychiatric patients" in Journal of Neural Transmission (2017),
https://doi.org/10.1007/s00702-017-1677-z . .

TY  - JOUR
AU  - Zolotarevski, Lidija
AU  - Jović, Milena
AU  - Popov Aleksandrov, Aleksandra
AU  - Milosavljević, Petar
AU  - Brajušković, Goran
AU  - Demenesku, Jelena
AU  - Mirkov, Ivana
AU  - Ninkov, Marina
AU  - Kataranovski, Dragan
AU  - Kataranovski, Milena
PY  - 2016
UR  - http://www.tandfonline.com/doi/full/10.3109/15569527.2015.1008701
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2971
AB  - CONTEXT: Skin is the target of both acute and chronic exposure to warfarin, coumarin anticoagulant. Single exposure of rat skin to this agent induces early (24 h following epicutaneous administration) local response which might be part of inflammatory/reparatory homeostatic program or introduction to pathological events in exposed skin. OBJECTIVE: To examine time-dependent changes in skin of rats exposed to epicutaneously applied warfarin. MATERIALS AND METHODS: The effect of low (10 μg) and high (100 μg) doses of warfarin on histologically evident changes of epidermis (epidermal thickness) and dermis (numbers of mesenchymal cells and dermal capillaries), skin cell proliferative activity (Ki67(+) and PCNA(+) cells) and apoptotic (TUNEL(+)) and necrotic (ultra structural appearance) cells was examined one, three and seven days after the application. RESULTS: Both warfarin doses affected the majority of skin cell activity, but with differential time-course of skin epidermal and dermal cells state/activity. The occurrence of necrotic/apoptotic epidermal and dermal cells was noted the first day after the application and the activities which point to tissue reparation/remodeling were observed seven days after skin exposure to this agent. DISCUSSION: The observed pattern of changes (early evidence of cell/tissue injury which was later followed by signs of cell activity characteristic for tissue reparation/remodeling) implied warfarin-induced toxicity in skin cells as stimulus for subsequent activities relevant for tissue homeostasis. CONCLUSION: The data presented provide new and additional information concerning skin responses to warfarin that gains access to this tissue.
T2  - Cutaneous and Ocular Toxicology
T2  - Cutaneous and Ocular Toxicology
T1  - Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.
IS  - 1
VL  - 35
DO  - 10.3109/15569527.2015.1008701
SP  - 41
EP  - 48
ER  - 

@article{
author = "Zolotarevski, Lidija and Jović, Milena and Popov Aleksandrov, Aleksandra and Milosavljević, Petar and Brajušković, Goran and Demenesku, Jelena and Mirkov, Ivana and Ninkov, Marina and Kataranovski, Dragan and Kataranovski, Milena",
year = "2016",
abstract = "CONTEXT: Skin is the target of both acute and chronic exposure to warfarin, coumarin anticoagulant. Single exposure of rat skin to this agent induces early (24 h following epicutaneous administration) local response which might be part of inflammatory/reparatory homeostatic program or introduction to pathological events in exposed skin. OBJECTIVE: To examine time-dependent changes in skin of rats exposed to epicutaneously applied warfarin. MATERIALS AND METHODS: The effect of low (10 μg) and high (100 μg) doses of warfarin on histologically evident changes of epidermis (epidermal thickness) and dermis (numbers of mesenchymal cells and dermal capillaries), skin cell proliferative activity (Ki67(+) and PCNA(+) cells) and apoptotic (TUNEL(+)) and necrotic (ultra structural appearance) cells was examined one, three and seven days after the application. RESULTS: Both warfarin doses affected the majority of skin cell activity, but with differential time-course of skin epidermal and dermal cells state/activity. The occurrence of necrotic/apoptotic epidermal and dermal cells was noted the first day after the application and the activities which point to tissue reparation/remodeling were observed seven days after skin exposure to this agent. DISCUSSION: The observed pattern of changes (early evidence of cell/tissue injury which was later followed by signs of cell activity characteristic for tissue reparation/remodeling) implied warfarin-induced toxicity in skin cells as stimulus for subsequent activities relevant for tissue homeostasis. CONCLUSION: The data presented provide new and additional information concerning skin responses to warfarin that gains access to this tissue.",
journal = "Cutaneous and Ocular Toxicology, Cutaneous and Ocular Toxicology",
title = "Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.",
number = "1",
volume = "35",
doi = "10.3109/15569527.2015.1008701",
pages = "41-48"
}

Zolotarevski, L., Jović, M., Popov Aleksandrov, A., Milosavljević, P., Brajušković, G., Demenesku, J., Mirkov, I., Ninkov, M., Kataranovski, D.,& Kataranovski, M.. (2016). Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.. in Cutaneous and Ocular Toxicology, 35(1), 41-48.
https://doi.org/10.3109/15569527.2015.1008701

Zolotarevski L, Jović M, Popov Aleksandrov A, Milosavljević P, Brajušković G, Demenesku J, Mirkov I, Ninkov M, Kataranovski D, Kataranovski M. Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity.. in Cutaneous and Ocular Toxicology. 2016;35(1):41-48.
doi:10.3109/15569527.2015.1008701 .

Zolotarevski, Lidija, Jović, Milena, Popov Aleksandrov, Aleksandra, Milosavljević, Petar, Brajušković, Goran, Demenesku, Jelena, Mirkov, Ivana, Ninkov, Marina, Kataranovski, Dragan, Kataranovski, Milena, "Skin response to epicutaneous application of anticoagulant rodenticide warfarin is characterized by differential time- and dose-dependent changes in cell activity." in Cutaneous and Ocular Toxicology, 35, no. 1 (2016):41-48,
https://doi.org/10.3109/15569527.2015.1008701 . .