TY  - JOUR
AU  - Ljujic, Mila
AU  - Mijatović, Sanja
AU  - Bulatović, Mirna
AU  - Mojić, Marija
AU  - Maksimović-Ivanić, Danijela
AU  - Radojkovic, Dragica
AU  - Topic, Aleksandra
PY  - 2016
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4166
AB  - Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein is encoded by the SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated that AAT has an anti-apoptotic property against non-malignant cells, we aimed to investigate whether AAT affects apoptosis in a colon cancer cell line (HCT116). The presence of AAT in the HCT116 cell culture antagonized cytotoxicity of blockers of MEK1/2, PI3K/Akt pathways as well as NF-κB. The dominantly recovered cell viability was observed in the co-treatment with MEK1/2 inhibitor U0126. In addition, it was revealed that AAT almost completely abolished U0126-induced apoptosis through maintenance of the autophagy process. Our study revealed for the first time that the observed cyto-protection triggered by AAT was accompanied by sustained autophagy which opposed apoptosis. These results may contribute to understanding of the role of AAT in cancer development and evaluation of efficacy of cancer therapy.
PB  - Moskva: Izdatelstvo Nauka
T2  - Molekuliarnaia biologiia
T1  - Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)
IS  - 1
VL  - 50
DO  - 10.7868/S0026898416010122
SP  - 174
EP  - 178
ER  - 

@article{
author = "Ljujic, Mila and Mijatović, Sanja and Bulatović, Mirna and Mojić, Marija and Maksimović-Ivanić, Danijela and Radojkovic, Dragica and Topic, Aleksandra",
year = "2016",
abstract = "Alpha-1-antitrypsin (AAT), an acute phase protein, is the principal circulatory anti-protease. This multifunctional protein is encoded by the SERPINA1 gene. Although AAT was recognised as a potential tumour marker, its role in cancer biology remains unknown. Given that it has been demonstrated that AAT has an anti-apoptotic property against non-malignant cells, we aimed to investigate whether AAT affects apoptosis in a colon cancer cell line (HCT116). The presence of AAT in the HCT116 cell culture antagonized cytotoxicity of blockers of MEK1/2, PI3K/Akt pathways as well as NF-κB. The dominantly recovered cell viability was observed in the co-treatment with MEK1/2 inhibitor U0126. In addition, it was revealed that AAT almost completely abolished U0126-induced apoptosis through maintenance of the autophagy process. Our study revealed for the first time that the observed cyto-protection triggered by AAT was accompanied by sustained autophagy which opposed apoptosis. These results may contribute to understanding of the role of AAT in cancer development and evaluation of efficacy of cancer therapy.",
publisher = "Moskva: Izdatelstvo Nauka",
journal = "Molekuliarnaia biologiia",
title = "Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)",
number = "1",
volume = "50",
doi = "10.7868/S0026898416010122",
pages = "174-178"
}

Ljujic, M., Mijatović, S., Bulatović, M., Mojić, M., Maksimović-Ivanić, D., Radojkovic, D.,& Topic, A.. (2016). Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116). in Molekuliarnaia biologiia
Moskva: Izdatelstvo Nauka., 50(1), 174-178.
https://doi.org/10.7868/S0026898416010122

Ljujic M, Mijatović S, Bulatović M, Mojić M, Maksimović-Ivanić D, Radojkovic D, Topic A. Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116). in Molekuliarnaia biologiia. 2016;50(1):174-178.
doi:10.7868/S0026898416010122 .

Ljujic, Mila, Mijatović, Sanja, Bulatović, Mirna, Mojić, Marija, Maksimović-Ivanić, Danijela, Radojkovic, Dragica, Topic, Aleksandra, "Alpha-1 Antitrypsin Affects U0126-Induced Cytotoxicity in Colon Cancer Cell Line (HCT116)" in Molekuliarnaia biologiia, 50, no. 1 (2016):174-178,
https://doi.org/10.7868/S0026898416010122 . .