TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Janjić, Marija
AU  - Prévide, Rafael Maso
AU  - Abebe, Daniel
AU  - Kucka, Marek
AU  - Stojilković, Stanko S.
PY  - 2019
UR  - https://www.frontiersin.org/articles/10.3389/fendo.2019.00248/full
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3363
AB  - Cell-matrix interactions play important roles in pituitary development, physiology, and pathogenesis. In other tissues, a family of non-collagenous proteins, termed SIBLINGs, are known to contribute to cell-matrix interactions. Anterior pituitary gland expresses two SIBLING genes, Dmp1 (dentin matrix protein-1) and Spp1 (secreted phosphoprotein-1) encoding DMP1 and osteopontin proteins, respectively, but their expression pattern and roles in pituitary functions have not been clarified. Here we provide novel evidence supporting the conclusion that Spp1/osteopontin, like Dmp1/DMP1, are expressed in gonadotrophs in a sex- and age-specific manner. Other anterior pituitary cell types do not express these genes. In contrast to Dmp1, Spp1 expression is higher in males; in females, the expression reaches the peak during the diestrus phase of estrous cycle. In further contrast to Dmp1 and marker genes for gonadotrophs, the expression of Spp1 is not regulated by gonadotropin-releasing hormone in vivo and in vitro. However, Spp1 expression increases progressively after pituitary cell dispersion in both female and male cultures. We may speculate that gonadotrophs signal to other pituitary cell types about changes in the structure of pituitary cell-matrix network by osteopontin, a function consistent with the role of this secretory protein in postnatal tissue remodeling, extracellular matrix reorganization after injury, and tumorigenesis.
PB  - Frontiers Media S.A.
T2  - Frontiers in Endocrinology
T1  - Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs
IS  - MAR
VL  - 10
VL  - 10.3389/fendo.2019.00248
DO  - 10.3389/fendo.2019.00248
SP  - 248
ER  - 

@article{
author = "Bjelobaba, Ivana and Janjić, Marija and Prévide, Rafael Maso and Abebe, Daniel and Kucka, Marek and Stojilković, Stanko S.",
year = "2019",
abstract = "Cell-matrix interactions play important roles in pituitary development, physiology, and pathogenesis. In other tissues, a family of non-collagenous proteins, termed SIBLINGs, are known to contribute to cell-matrix interactions. Anterior pituitary gland expresses two SIBLING genes, Dmp1 (dentin matrix protein-1) and Spp1 (secreted phosphoprotein-1) encoding DMP1 and osteopontin proteins, respectively, but their expression pattern and roles in pituitary functions have not been clarified. Here we provide novel evidence supporting the conclusion that Spp1/osteopontin, like Dmp1/DMP1, are expressed in gonadotrophs in a sex- and age-specific manner. Other anterior pituitary cell types do not express these genes. In contrast to Dmp1, Spp1 expression is higher in males; in females, the expression reaches the peak during the diestrus phase of estrous cycle. In further contrast to Dmp1 and marker genes for gonadotrophs, the expression of Spp1 is not regulated by gonadotropin-releasing hormone in vivo and in vitro. However, Spp1 expression increases progressively after pituitary cell dispersion in both female and male cultures. We may speculate that gonadotrophs signal to other pituitary cell types about changes in the structure of pituitary cell-matrix network by osteopontin, a function consistent with the role of this secretory protein in postnatal tissue remodeling, extracellular matrix reorganization after injury, and tumorigenesis.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Endocrinology",
title = "Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs",
number = "MAR",
volume = "10, 10.3389/fendo.2019.00248",
doi = "10.3389/fendo.2019.00248",
pages = "248"
}

Bjelobaba, I., Janjić, M., Prévide, R. M., Abebe, D., Kucka, M.,& Stojilković, S. S.. (2019). Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs. in Frontiers in Endocrinology
Frontiers Media S.A.., 10(MAR), 248.
https://doi.org/10.3389/fendo.2019.00248

Bjelobaba I, Janjić M, Prévide RM, Abebe D, Kucka M, Stojilković SS. Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs. in Frontiers in Endocrinology. 2019;10(MAR):248.
doi:10.3389/fendo.2019.00248 .

Bjelobaba, Ivana, Janjić, Marija, Prévide, Rafael Maso, Abebe, Daniel, Kucka, Marek, Stojilković, Stanko S., "Distinct Expression Patterns of Osteopontin and Dentin Matrix Protein 1 Genes in Pituitary Gonadotrophs" in Frontiers in Endocrinology, 10, no. MAR (2019):248,
https://doi.org/10.3389/fendo.2019.00248 . .

TY  - JOUR
AU  - Janjić, Marija
AU  - Prévide, Rafael M.
AU  - Fletcher, Patrick A.
AU  - Sherman, Arthur
AU  - Smiljanić, Kosara
AU  - Abebe, Daniel
AU  - Bjelobaba, Ivana
AU  - Stojilković, Stanko S.
PY  - 2019
UR  - http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC6934515
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3595
AB  - Continuous, as opposed to pulsatile, delivery of hypothalamic gonadotropin-releasing hormone (GnRH) leads to a marked decrease in secretion of pituitary gonadotropins LH and FSH and impairment of reproductive function. Here we studied the expression profile of gonadotropin subunit and GnRH receptor genes in rat pituitary in vitro and in vivo to clarify their expression profiles in the absence and continuous presence of GnRH. Culturing of pituitary cells in GnRH-free conditions downregulated Fshb, Cga, and Gnrhr expression, whereas continuous treatment with GnRH agonists upregulated Cga expression progressively and Gnrhr and Fshb expression transiently, accompanied by a prolonged blockade of Fshb but not Gnrhr expression. In contrast, Lhb expression was relatively insensitive to loss of endogenous GnRH and continuous treatment with GnRH, probably reflecting the status of Egr1 and Nr5a1 expression. Similar patterns of responses were observed in vivo after administration of a GnRH agonist. However, continuous treatment with GnRH stimulated LH secretion in vitro and in vivo, leading to decrease in LH cell content despite high basal Lhb expression. These data suggest that blockade of Fshb expression and depletion of the LH secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous GnRH treatment.
T2  - Scientific Reports
T1  - Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.
IS  - 1
VL  - 9
DO  - 10.1038/s41598-019-56480-1
SP  - 20098
ER  - 

@article{
author = "Janjić, Marija and Prévide, Rafael M. and Fletcher, Patrick A. and Sherman, Arthur and Smiljanić, Kosara and Abebe, Daniel and Bjelobaba, Ivana and Stojilković, Stanko S.",
year = "2019",
abstract = "Continuous, as opposed to pulsatile, delivery of hypothalamic gonadotropin-releasing hormone (GnRH) leads to a marked decrease in secretion of pituitary gonadotropins LH and FSH and impairment of reproductive function. Here we studied the expression profile of gonadotropin subunit and GnRH receptor genes in rat pituitary in vitro and in vivo to clarify their expression profiles in the absence and continuous presence of GnRH. Culturing of pituitary cells in GnRH-free conditions downregulated Fshb, Cga, and Gnrhr expression, whereas continuous treatment with GnRH agonists upregulated Cga expression progressively and Gnrhr and Fshb expression transiently, accompanied by a prolonged blockade of Fshb but not Gnrhr expression. In contrast, Lhb expression was relatively insensitive to loss of endogenous GnRH and continuous treatment with GnRH, probably reflecting the status of Egr1 and Nr5a1 expression. Similar patterns of responses were observed in vivo after administration of a GnRH agonist. However, continuous treatment with GnRH stimulated LH secretion in vitro and in vivo, leading to decrease in LH cell content despite high basal Lhb expression. These data suggest that blockade of Fshb expression and depletion of the LH secretory pool are two major factors accounting for weakening of the gonadotroph secretory function during continuous GnRH treatment.",
journal = "Scientific Reports",
title = "Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.",
number = "1",
volume = "9",
doi = "10.1038/s41598-019-56480-1",
pages = "20098"
}

Janjić, M., Prévide, R. M., Fletcher, P. A., Sherman, A., Smiljanić, K., Abebe, D., Bjelobaba, I.,& Stojilković, S. S.. (2019). Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.. in Scientific Reports, 9(1), 20098.
https://doi.org/10.1038/s41598-019-56480-1

Janjić M, Prévide RM, Fletcher PA, Sherman A, Smiljanić K, Abebe D, Bjelobaba I, Stojilković SS. Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo.. in Scientific Reports. 2019;9(1):20098.
doi:10.1038/s41598-019-56480-1 .

Janjić, Marija, Prévide, Rafael M., Fletcher, Patrick A., Sherman, Arthur, Smiljanić, Kosara, Abebe, Daniel, Bjelobaba, Ivana, Stojilković, Stanko S., "Divergent expression patterns of pituitary gonadotropin subunit and GnRH receptor genes to continuous GnRH in vitro and in vivo." in Scientific Reports, 9, no. 1 (2019):20098,
https://doi.org/10.1038/s41598-019-56480-1 . .