TY  - JOUR
AU  - Bjelobaba, Ivana
AU  - Janjić, Marija
AU  - Tavcar, Jovana S.
AU  - Kucka, Marek
AU  - Tomić, Melanija
AU  - Stojilkovic, Stanko S.
PY  - 2016
UR  - http://linkinghub.elsevier.com/retrieve/pii/S0303720716303471
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2523
AB  - Hypothalamic GnRH together with gonadal steroids and activins/inhibin regulate its receptor gene (Gnrhr) expression in vivo, which leads to crucial changes in GnRHR numbers on the plasma membrane. This is accompanied by alterations in the gonadotroph sensitivity and responsiveness during physiologically relevant situations. Here we investigated basal and GnRH-regulated Gnrhr expression in rodent pituitary gonadotrophs in vitro. In pituitary cells from adult animals cultured in the absence of GnRH and steroid hormones, the Gnrhr expression was progressively reduced but not completely abolished. The basal Gnrhr expression was also operative in LβT2 immortalized gonadotrophs never exposed to GnRH. In both cell types, basal transcription was sufficient for the expression of functional GnRHRs. Continuous application of GnRH transiently elevated the Gnrhr expression in cultured pituitary cells followed by a sustained fall without affecting basal transcription. Both basal and regulated Gnrhr transcriptions were dependent on the protein kinase C signaling pathway. The GnRH-regulated Gnrhr expression was not operative in embryonal pituitary and LβT2 cells and was established neonatally, the sex-specific response patterns were formed at the juvenile-peripubertal stage and there was a strong correlation between basal and regulated gene expression during development. Thus, the age-dependent basal and regulated Gnrhr transcription could account for the initial blockade and subsequent activation of the reproductive system during development.
PB  - Elsevier Ireland Ltd
T2  - Molecular and Cellular Endocrinology
T1  - The relationship between basal and regulated Gnrhr expression in rodent pituitary gonadotrophs
VL  - 437
DO  - 10.1016/j.mce.2016.08.040
SP  - 302
EP  - 311
ER  - 

@article{
author = "Bjelobaba, Ivana and Janjić, Marija and Tavcar, Jovana S. and Kucka, Marek and Tomić, Melanija and Stojilkovic, Stanko S.",
year = "2016",
abstract = "Hypothalamic GnRH together with gonadal steroids and activins/inhibin regulate its receptor gene (Gnrhr) expression in vivo, which leads to crucial changes in GnRHR numbers on the plasma membrane. This is accompanied by alterations in the gonadotroph sensitivity and responsiveness during physiologically relevant situations. Here we investigated basal and GnRH-regulated Gnrhr expression in rodent pituitary gonadotrophs in vitro. In pituitary cells from adult animals cultured in the absence of GnRH and steroid hormones, the Gnrhr expression was progressively reduced but not completely abolished. The basal Gnrhr expression was also operative in LβT2 immortalized gonadotrophs never exposed to GnRH. In both cell types, basal transcription was sufficient for the expression of functional GnRHRs. Continuous application of GnRH transiently elevated the Gnrhr expression in cultured pituitary cells followed by a sustained fall without affecting basal transcription. Both basal and regulated Gnrhr transcriptions were dependent on the protein kinase C signaling pathway. The GnRH-regulated Gnrhr expression was not operative in embryonal pituitary and LβT2 cells and was established neonatally, the sex-specific response patterns were formed at the juvenile-peripubertal stage and there was a strong correlation between basal and regulated gene expression during development. Thus, the age-dependent basal and regulated Gnrhr transcription could account for the initial blockade and subsequent activation of the reproductive system during development.",
publisher = "Elsevier Ireland Ltd",
journal = "Molecular and Cellular Endocrinology",
title = "The relationship between basal and regulated Gnrhr expression in rodent pituitary gonadotrophs",
volume = "437",
doi = "10.1016/j.mce.2016.08.040",
pages = "302-311"
}

Bjelobaba, I., Janjić, M., Tavcar, J. S., Kucka, M., Tomić, M.,& Stojilkovic, S. S.. (2016). The relationship between basal and regulated Gnrhr expression in rodent pituitary gonadotrophs. in Molecular and Cellular Endocrinology
Elsevier Ireland Ltd., 437, 302-311.
https://doi.org/10.1016/j.mce.2016.08.040

Bjelobaba I, Janjić M, Tavcar JS, Kucka M, Tomić M, Stojilkovic SS. The relationship between basal and regulated Gnrhr expression in rodent pituitary gonadotrophs. in Molecular and Cellular Endocrinology. 2016;437:302-311.
doi:10.1016/j.mce.2016.08.040 .

Bjelobaba, Ivana, Janjić, Marija, Tavcar, Jovana S., Kucka, Marek, Tomić, Melanija, Stojilkovic, Stanko S., "The relationship between basal and regulated Gnrhr expression in rodent pituitary gonadotrophs" in Molecular and Cellular Endocrinology, 437 (2016):302-311,
https://doi.org/10.1016/j.mce.2016.08.040 . .