TY  - JOUR
AU  - Drača, Dijana
AU  - Marković, Milan
AU  - Gozzi, Marta
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Hey-Hawkins, Evamarie
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4425
AB  - Gliomas and glioblastomas are very aggressive forms of brain tumors, prone to the devel opment of a multitude of resistance mechanisms to therapeutic treatments, including cytoprotective
autophagy. In this work, we investigated the role and mechanism of action of the combination of a
ruthenacarborane derivative with 8-hydroxyquinoline (8-HQ), linked via an ester bond (complex 2),
in rat astrocytoma C6 and human glioma U251 cells, in comparison with the two compounds alone,
i.e., the free carboxylic acid (complex 1) and 8-HQ, and their non-covalent combination ([1 + 8-HQ],
in 1:1 molar ratio). We found that only complex 2 was able to significantly affect cellular viability
in glioma U251 cells (IC50 11.4 µM) via inhibition of the autophagic machinery, most likely acting
at the early stages of the autophagic cascade. Contrary to 8-HQ alone, complex 2 was also able
to impair cellular viability under conditions of glucose deprivation. We thus suggest different
mechanisms of action of ruthenacarborane complex 2 than purely organic quinoline-based drugs,
making complex 2 a very attractive candidate for evading the known resistances of brain tumors to
chloroquine-based therapies.
PB  - Basel: MDPI
T2  - Molecules
T1  - Ruthenacarborane and Quinoline: A Promising Combination for the Treatment of Brain Tumors
IS  - 13
VL  - 26
DO  - 10.3390/molecules26133801
SP  - 3801
ER  - 

@article{
author = "Drača, Dijana and Marković, Milan and Gozzi, Marta and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Hey-Hawkins, Evamarie",
year = "2021",
abstract = "Gliomas and glioblastomas are very aggressive forms of brain tumors, prone to the devel opment of a multitude of resistance mechanisms to therapeutic treatments, including cytoprotective
autophagy. In this work, we investigated the role and mechanism of action of the combination of a
ruthenacarborane derivative with 8-hydroxyquinoline (8-HQ), linked via an ester bond (complex 2),
in rat astrocytoma C6 and human glioma U251 cells, in comparison with the two compounds alone,
i.e., the free carboxylic acid (complex 1) and 8-HQ, and their non-covalent combination ([1 + 8-HQ],
in 1:1 molar ratio). We found that only complex 2 was able to significantly affect cellular viability
in glioma U251 cells (IC50 11.4 µM) via inhibition of the autophagic machinery, most likely acting
at the early stages of the autophagic cascade. Contrary to 8-HQ alone, complex 2 was also able
to impair cellular viability under conditions of glucose deprivation. We thus suggest different
mechanisms of action of ruthenacarborane complex 2 than purely organic quinoline-based drugs,
making complex 2 a very attractive candidate for evading the known resistances of brain tumors to
chloroquine-based therapies.",
publisher = "Basel: MDPI",
journal = "Molecules",
title = "Ruthenacarborane and Quinoline: A Promising Combination for the Treatment of Brain Tumors",
number = "13",
volume = "26",
doi = "10.3390/molecules26133801",
pages = "3801"
}

Drača, D., Marković, M., Gozzi, M., Mijatović, S., Maksimović-Ivanić, D.,& Hey-Hawkins, E.. (2021). Ruthenacarborane and Quinoline: A Promising Combination for the Treatment of Brain Tumors. in Molecules
Basel: MDPI., 26(13), 3801.
https://doi.org/10.3390/molecules26133801

Drača D, Marković M, Gozzi M, Mijatović S, Maksimović-Ivanić D, Hey-Hawkins E. Ruthenacarborane and Quinoline: A Promising Combination for the Treatment of Brain Tumors. in Molecules. 2021;26(13):3801.
doi:10.3390/molecules26133801 .

Drača, Dijana, Marković, Milan, Gozzi, Marta, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Hey-Hawkins, Evamarie, "Ruthenacarborane and Quinoline: A Promising Combination for the Treatment of Brain Tumors" in Molecules, 26, no. 13 (2021):3801,
https://doi.org/10.3390/molecules26133801 . .

TY  - JOUR
AU  - Marković, Milan
PY  - 2020
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4178
UR  - http://hrana-ishrana.org/wp-content/uploads/2021/03/Broj-2-Vol-61-2020.pdf
AB  - COVID-19 je infektivna bolest izazvana virusom SARS-CoV-2 koji pripada
grupi Coronaviridae. Simptomi bolesti su prvenstveno prisutni u obliku respiratornog
sindroma sličnog simptomima do kojih dovode drugi respiratorni
virusi poput virusa gripa, kao i virusa koji dovode do oboljenja poznatih
pod imenom SARS i MERS. Međutim, na osnovu kratkog kliničkog iskustva,
pokazalo se da COVID-19 utiče na sve organske sisteme ali je patogeneza
bolesti i dalje nejasna. Blokada u razmeni gasova, sistemska hipoksija i poremećaji
u koagulaciji prate najtežu kliničku sliku. Pored toga, ovaj virus pripada
grupi RNA virusa koji dele zajedničke karakteristike sa virusom HIV-
a,
što ga čini veoma izazovnim u pogledu terapije savremenim medicinskim
pristupima. Trenutno se širom sveta vode polemike o tome kako se boriti
protiv ove epidemije. Pored higijenskih mera i mera bezbednog rastojanja
i izolacije, postoji važna polemika oko profilaktičkih vakcina. U ovom preglednom
članku pruža se uvid u sve aspekte vakcinacije i metode za njihov
dizajn zasnovane na tehnologijama koje poseduje savremena nauka sa
posebnim osvrtom na genetske (DNK, RNK) vakcine, i postavlja pitanje da
li postoji opravdanost njihove urgentne primene u slučaju pandemije COVID-
19.
AB  - COVID-19 is an infectious disease caused by SARS-CoV-2 virus which
belongs to the Coronaviridae group. The symptoms of the disease are
primarily present in the form of respiratory syndrome similar to the
symptoms of other respiratory viruses such as influenza virus but also
the very common SARS-CoV-1 and MERS-CoV viruses known from the
recent past. However, based on short clinical experience, it was found
that apart from lungs COVID-19 affects all organ systems with unclear
pathogenesis. Respiratory failure followed by systemic hypoxia and coagulation
disorders is hallmarks of severe pathology. In addition, this
virus belongs to the group of RNA viruses that share common characteristics
with the HIV virus, which makes it very challenging for the therapy
of modern medicine. There is currently controversy around the world
about how to combat this epidemic. In addition to hygienic measures
and measures of safe distance and isolation, there is an intense controversy
about prophylactic vaccines. In this review article, the insight
into all aspects of vaccination and methods for their design based on
technological tools possessed by modern science with special reference
to genetic (DNA, RNA) vaccines is provided, and the question arises of
whether there is a justification of novel technology urgent application
in the event of a COVID-19 pandemic.
PB  - Belgrade: Serbian Nutrition Society
T2  - Hrana i ishrana
T1  - COVID-19 as milestone for the use of new vaccine types
T1  - COVID-19 kao prekretnica za upotrebu novih vrsta vakcina
IS  - 1
VL  - 61
DO  - 10.5937/hraIsh2001001M
SP  - 1
EP  - 18
ER  - 

@article{
author = "Marković, Milan",
year = "2020",
abstract = "COVID-19 je infektivna bolest izazvana virusom SARS-CoV-2 koji pripada
grupi Coronaviridae. Simptomi bolesti su prvenstveno prisutni u obliku respiratornog
sindroma sličnog simptomima do kojih dovode drugi respiratorni
virusi poput virusa gripa, kao i virusa koji dovode do oboljenja poznatih
pod imenom SARS i MERS. Međutim, na osnovu kratkog kliničkog iskustva,
pokazalo se da COVID-19 utiče na sve organske sisteme ali je patogeneza
bolesti i dalje nejasna. Blokada u razmeni gasova, sistemska hipoksija i poremećaji
u koagulaciji prate najtežu kliničku sliku. Pored toga, ovaj virus pripada
grupi RNA virusa koji dele zajedničke karakteristike sa virusom HIV-
a,
što ga čini veoma izazovnim u pogledu terapije savremenim medicinskim
pristupima. Trenutno se širom sveta vode polemike o tome kako se boriti
protiv ove epidemije. Pored higijenskih mera i mera bezbednog rastojanja
i izolacije, postoji važna polemika oko profilaktičkih vakcina. U ovom preglednom
članku pruža se uvid u sve aspekte vakcinacije i metode za njihov
dizajn zasnovane na tehnologijama koje poseduje savremena nauka sa
posebnim osvrtom na genetske (DNK, RNK) vakcine, i postavlja pitanje da
li postoji opravdanost njihove urgentne primene u slučaju pandemije COVID-
19., COVID-19 is an infectious disease caused by SARS-CoV-2 virus which
belongs to the Coronaviridae group. The symptoms of the disease are
primarily present in the form of respiratory syndrome similar to the
symptoms of other respiratory viruses such as influenza virus but also
the very common SARS-CoV-1 and MERS-CoV viruses known from the
recent past. However, based on short clinical experience, it was found
that apart from lungs COVID-19 affects all organ systems with unclear
pathogenesis. Respiratory failure followed by systemic hypoxia and coagulation
disorders is hallmarks of severe pathology. In addition, this
virus belongs to the group of RNA viruses that share common characteristics
with the HIV virus, which makes it very challenging for the therapy
of modern medicine. There is currently controversy around the world
about how to combat this epidemic. In addition to hygienic measures
and measures of safe distance and isolation, there is an intense controversy
about prophylactic vaccines. In this review article, the insight
into all aspects of vaccination and methods for their design based on
technological tools possessed by modern science with special reference
to genetic (DNA, RNA) vaccines is provided, and the question arises of
whether there is a justification of novel technology urgent application
in the event of a COVID-19 pandemic.",
publisher = "Belgrade: Serbian Nutrition Society",
journal = "Hrana i ishrana",
title = "COVID-19 as milestone for the use of new vaccine types, COVID-19 kao prekretnica za upotrebu novih vrsta vakcina",
number = "1",
volume = "61",
doi = "10.5937/hraIsh2001001M",
pages = "1-18"
}

Marković, M.. (2020). COVID-19 as milestone for the use of new vaccine types. in Hrana i ishrana
Belgrade: Serbian Nutrition Society., 61(1), 1-18.
https://doi.org/10.5937/hraIsh2001001M

Marković M. COVID-19 as milestone for the use of new vaccine types. in Hrana i ishrana. 2020;61(1):1-18.
doi:10.5937/hraIsh2001001M .

Marković, Milan, "COVID-19 as milestone for the use of new vaccine types" in Hrana i ishrana, 61, no. 1 (2020):1-18,
https://doi.org/10.5937/hraIsh2001001M . .

TY  - CONF
AU  - Marković, Milan
AU  - Marković, Katarina
AU  - Mirkov, Ivana
AU  - Tucović, Dina
AU  - Mijatović, Sanja
AU  - Maksimović-Ivanić, Danijela
AU  - Popov Aleksandrov, Aleksandra
PY  - 2019
UR  - https://www.isos.rs/congress2019-abstract-book
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3580
UR  - https://radar.ibiss.bg.ac.rs/handle/handle/123456789/3558
AB  - Cadmium (Cd) is one of the most cytotoxic agents and environmental contaminants, which can cause serial health problems in various organs such as liver, kidney, testis, bone, nervous tissue and immune system. Furthermore, Cd is classified as a human and animal carcinogen agent. Despite the fact that Cd accumulates in the skin, there is a lack of evidence about its involvement in skin cancer biology. Skin cancer is one of the most common cancers worldwide, with melanoma as the most lethal type. Based on that, we set up the study to examine the effect of cadmium on the B16 melanoma cell line, measuring viability by MTT and crystal violet (CV) tests, proliferation rate (CFSE), ROS production (DHR), migration (scratch test) and adhesion on matrigel. The results showed that non-cytotoxic doses of cadmium (ranging from 0,3-2,5µM) increased viability, proliferation, and migration of B16 cells, while at the same time slightly promoted ROS production. Otherwise, pronounced oxidative stress induced by higher doses (ranging from 5-20µM) led to cell death. Summary, these results showed that low doses of cadmium could upregulate B16 melanoma cell line growth and migration probably through moderate ROS/RNS generation and thus their ability to modulate relevant signaling pathways involved in cancer progression. Considering that, underlying mechanisms of Cd impact on melanoma cell lines proliferation, migration and invasiveness needs to be clarified in vitro, as well as its relevance for in vivo system.
PB  - Belgrade: University of Belgrade Institute for Biological Research "Siniša Stanković" National Institute of Republic of Serbia
PB  - Belgrade: Immunological Society of Serbia
C3  - Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book; 2019 Dec 6-8; Belgrade, Serbia
T1  - Effect of non-cytotoxic doses of cadmium on the B16 melanoma cell line
SP  - 123
EP  - 123
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_3580
ER  - 

@conference{
author = "Marković, Milan and Marković, Katarina and Mirkov, Ivana and Tucović, Dina and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Popov Aleksandrov, Aleksandra",
year = "2019",
abstract = "Cadmium (Cd) is one of the most cytotoxic agents and environmental contaminants, which can cause serial health problems in various organs such as liver, kidney, testis, bone, nervous tissue and immune system. Furthermore, Cd is classified as a human and animal carcinogen agent. Despite the fact that Cd accumulates in the skin, there is a lack of evidence about its involvement in skin cancer biology. Skin cancer is one of the most common cancers worldwide, with melanoma as the most lethal type. Based on that, we set up the study to examine the effect of cadmium on the B16 melanoma cell line, measuring viability by MTT and crystal violet (CV) tests, proliferation rate (CFSE), ROS production (DHR), migration (scratch test) and adhesion on matrigel. The results showed that non-cytotoxic doses of cadmium (ranging from 0,3-2,5µM) increased viability, proliferation, and migration of B16 cells, while at the same time slightly promoted ROS production. Otherwise, pronounced oxidative stress induced by higher doses (ranging from 5-20µM) led to cell death. Summary, these results showed that low doses of cadmium could upregulate B16 melanoma cell line growth and migration probably through moderate ROS/RNS generation and thus their ability to modulate relevant signaling pathways involved in cancer progression. Considering that, underlying mechanisms of Cd impact on melanoma cell lines proliferation, migration and invasiveness needs to be clarified in vitro, as well as its relevance for in vivo system.",
publisher = "Belgrade: University of Belgrade Institute for Biological Research "Siniša Stanković" National Institute of Republic of Serbia, Belgrade: Immunological Society of Serbia",
journal = "Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book; 2019 Dec 6-8; Belgrade, Serbia",
title = "Effect of non-cytotoxic doses of cadmium on the B16 melanoma cell line",
pages = "123-123",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_3580"
}

Marković, M., Marković, K., Mirkov, I., Tucović, D., Mijatović, S., Maksimović-Ivanić, D.,& Popov Aleksandrov, A.. (2019). Effect of non-cytotoxic doses of cadmium on the B16 melanoma cell line. in Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book; 2019 Dec 6-8; Belgrade, Serbia
Belgrade: University of Belgrade Institute for Biological Research "Siniša Stanković" National Institute of Republic of Serbia., 123-123.
https://hdl.handle.net/21.15107/rcub_ibiss_3580

Marković M, Marković K, Mirkov I, Tucović D, Mijatović S, Maksimović-Ivanić D, Popov Aleksandrov A. Effect of non-cytotoxic doses of cadmium on the B16 melanoma cell line. in Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book; 2019 Dec 6-8; Belgrade, Serbia. 2019;:123-123.
https://hdl.handle.net/21.15107/rcub_ibiss_3580 .

Marković, Milan, Marković, Katarina, Mirkov, Ivana, Tucović, Dina, Mijatović, Sanja, Maksimović-Ivanić, Danijela, Popov Aleksandrov, Aleksandra, "Effect of non-cytotoxic doses of cadmium on the B16 melanoma cell line" in Saksida T, Stanisavljević S, Miljković Đ, editors. Immunology at the Confluence of Multidisciplinary Approaches : abstract book; 2019 Dec 6-8; Belgrade, Serbia (2019):123-123,
https://hdl.handle.net/21.15107/rcub_ibiss_3580 .