TY  - JOUR
AU  - Nedeljković, Nadežda
AU  - Bjelobaba, Ivana
AU  - Dacić, Sanja
AU  - Lavrnja, Irena
AU  - Peković, Sanja
AU  - Savić, Danijela
AU  - Vještica, Aleksandar
AU  - Rakić, Ljubisav
AU  - Stojiljković, Mirjana
PY  - 2006
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1016/j.cellbi.2006.03.001
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3464
UR  - https://www.sciencedirect.com/science/article/abs/pii/S1065699506000643
AB  - The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 50-nucleotidase were assessed by measuring the levels of ATP, ADP and AMP hydrolysis in the crude membrane preparations obtained from injured left cortex, right cortex, left and right caudate nucleus, whole hippocampus and cerebellum. Significant increase in NTPDase and ecto 50-nucleotidase activities was observed in the injured cortex following CSI, whereas in other brain areas only an increase in ecto 50-nucleotidase activity was seen. Immunohistochemical analysis performed using antibodies specific to NTPDase 1 and ecto 50-nucleotidase demonstrated that CSI induced sig-nificant changes in enzyme expression around the injury site. Immunoreactivity patterns obtained for NTPDase 1 and ecto 50-nucleotidase were compared with those obtained for glial fibrillary acidic protein, as a marker of astrocytes and complement receptor type 3 (OX42), as a marker of microglia. Results suggest that up-regulation of ectonucleotidase after CSI is catalyzed by cells that activate in response to injury, i.e. cells immunopositive for NTPDase 1 were predominantly microglial cells, whereas cells immunopositive for ecto 50-nucleotidase were predominantly astrocytes.
PB  - Elsevier
T2  - Cell Biology International
T1  - Up-regulation of ectonucleotidase activity after cortical stab injury in rats
IS  - 6
VL  - 30
DO  - 10.1016/j.cellbi.2006.03.001
SP  - 541
EP  - 546
ER  - 

@article{
author = "Nedeljković, Nadežda and Bjelobaba, Ivana and Dacić, Sanja and Lavrnja, Irena and Peković, Sanja and Savić, Danijela and Vještica, Aleksandar and Rakić, Ljubisav and Stojiljković, Mirjana",
year = "2006",
abstract = "The objective of this study was to examine the changes in the activity and expression of ectonucleotidase enzymes in the model of unilateral cortical stab injury (CSI) in rat. The activities of ecto-nucleoside triphosphate diphosphohydrolase 1 (NTPDase 1) and ecto 50-nucleotidase were assessed by measuring the levels of ATP, ADP and AMP hydrolysis in the crude membrane preparations obtained from injured left cortex, right cortex, left and right caudate nucleus, whole hippocampus and cerebellum. Significant increase in NTPDase and ecto 50-nucleotidase activities was observed in the injured cortex following CSI, whereas in other brain areas only an increase in ecto 50-nucleotidase activity was seen. Immunohistochemical analysis performed using antibodies specific to NTPDase 1 and ecto 50-nucleotidase demonstrated that CSI induced sig-nificant changes in enzyme expression around the injury site. Immunoreactivity patterns obtained for NTPDase 1 and ecto 50-nucleotidase were compared with those obtained for glial fibrillary acidic protein, as a marker of astrocytes and complement receptor type 3 (OX42), as a marker of microglia. Results suggest that up-regulation of ectonucleotidase after CSI is catalyzed by cells that activate in response to injury, i.e. cells immunopositive for NTPDase 1 were predominantly microglial cells, whereas cells immunopositive for ecto 50-nucleotidase were predominantly astrocytes.",
publisher = "Elsevier",
journal = "Cell Biology International",
title = "Up-regulation of ectonucleotidase activity after cortical stab injury in rats",
number = "6",
volume = "30",
doi = "10.1016/j.cellbi.2006.03.001",
pages = "541-546"
}

Nedeljković, N., Bjelobaba, I., Dacić, S., Lavrnja, I., Peković, S., Savić, D., Vještica, A., Rakić, L.,& Stojiljković, M.. (2006). Up-regulation of ectonucleotidase activity after cortical stab injury in rats. in Cell Biology International
Elsevier., 30(6), 541-546.
https://doi.org/10.1016/j.cellbi.2006.03.001

Nedeljković N, Bjelobaba I, Dacić S, Lavrnja I, Peković S, Savić D, Vještica A, Rakić L, Stojiljković M. Up-regulation of ectonucleotidase activity after cortical stab injury in rats. in Cell Biology International. 2006;30(6):541-546.
doi:10.1016/j.cellbi.2006.03.001 .

Nedeljković, Nadežda, Bjelobaba, Ivana, Dacić, Sanja, Lavrnja, Irena, Peković, Sanja, Savić, Danijela, Vještica, Aleksandar, Rakić, Ljubisav, Stojiljković, Mirjana, "Up-regulation of ectonucleotidase activity after cortical stab injury in rats" in Cell Biology International, 30, no. 6 (2006):541-546,
https://doi.org/10.1016/j.cellbi.2006.03.001 . .