TY  - CHAP
AU  - Dragoj, Miodrag
AU  - Stojkovska, Jasmina
AU  - Jovanović Stojanov, Sofija
AU  - Obradović, Bojana
AU  - Pešić, Milica
AU  - Baiocchi, Marta
PY  - 2022
UR  - https://link.springer.com/10.1007/978-1-0716-2513-2_1
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/5093
AB  - 3D cultures of cancer cells enable better mimicking of physiological conditions compared to traditional monolayer 2D cultures. Here we describe alginate scaffold-based model that can be used in both static and biomimetic conditions for studying drug sensitivity in cancer cells and multidrug resistance (MDR) mechanisms. This 3D culture model resembles in vivo conditions and provides relevant and reproducible results. It is easy to set up and allows for facile manipulation for downstream analyses. All these remarkable features make this 3D culture model a promising tool in drug discovery and cancer cell biology research.
PB  - Humana Press Inc.
PB  - New York: Humana Press Inc.
T2  - Cancer Drug Resistance: Methods and Protocols
T1  - A 3D Biomimetic System for Testing Anticancer Drug Sensitivity.
VL  - 2535
DO  - 10.1007/978-1-0716-2513-2_1
SP  - 1
EP  - 9
ER  - 

@inbook{
author = "Dragoj, Miodrag and Stojkovska, Jasmina and Jovanović Stojanov, Sofija and Obradović, Bojana and Pešić, Milica and Baiocchi, Marta",
year = "2022",
abstract = "3D cultures of cancer cells enable better mimicking of physiological conditions compared to traditional monolayer 2D cultures. Here we describe alginate scaffold-based model that can be used in both static and biomimetic conditions for studying drug sensitivity in cancer cells and multidrug resistance (MDR) mechanisms. This 3D culture model resembles in vivo conditions and provides relevant and reproducible results. It is easy to set up and allows for facile manipulation for downstream analyses. All these remarkable features make this 3D culture model a promising tool in drug discovery and cancer cell biology research.",
publisher = "Humana Press Inc., New York: Humana Press Inc.",
journal = "Cancer Drug Resistance: Methods and Protocols",
booktitle = "A 3D Biomimetic System for Testing Anticancer Drug Sensitivity.",
volume = "2535",
doi = "10.1007/978-1-0716-2513-2_1",
pages = "1-9"
}

Dragoj, M., Stojkovska, J., Jovanović Stojanov, S., Obradović, B., Pešić, M.,& Baiocchi, M.. (2022). A 3D Biomimetic System for Testing Anticancer Drug Sensitivity.. in Cancer Drug Resistance: Methods and Protocols
Humana Press Inc.., 2535, 1-9.
https://doi.org/10.1007/978-1-0716-2513-2_1

Dragoj M, Stojkovska J, Jovanović Stojanov S, Obradović B, Pešić M, Baiocchi M. A 3D Biomimetic System for Testing Anticancer Drug Sensitivity.. in Cancer Drug Resistance: Methods and Protocols. 2022;2535:1-9.
doi:10.1007/978-1-0716-2513-2_1 .

Dragoj, Miodrag, Stojkovska, Jasmina, Jovanović Stojanov, Sofija, Obradović, Bojana, Pešić, Milica, Baiocchi, Marta, "A 3D Biomimetic System for Testing Anticancer Drug Sensitivity." in Cancer Drug Resistance: Methods and Protocols, 2535 (2022):1-9,
https://doi.org/10.1007/978-1-0716-2513-2_1 . .

TY  - CONF
AU  - Jovanović Stojanov, Sofija
AU  - Dragoj, Miodrag
AU  - Jovanović, Mirna
AU  - Stojkovska, Jasmina
AU  - Stankovic, Tijana
AU  - Dinić, Jelena
AU  - Podolski-Renić, Ana
AU  - Stepanović, Ana
AU  - Obradović, Bojana
AU  - Pešić, Milica
PY  - 2021
UR  - https://www.eacr.org/login.php?referrer=%2Fconference%2FGoodbyeflatbiology2021virtual%2Fdigital-abstracts%2Fdetail%2F4208
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4904
AB  - Glioblastoma (GMB) is the most common and aggressive primary malignant brain tumor. Median life expectancy with the only clinically approved treatment option, Stupp protocol, is 11-15 months. This protocol involves surgical resection followed by 6 weeks of radiation and chemotherapy and then 6 cycles of 5-day chemotherapy treatment and 23 days of recovery. The only approved chemotherapeutic drug currently used is temozolomide (TMZ) that improves patients' survival for 2.5 months compared to radiotherapy alone. Such limited therapeutic options could be partly due to the lack of appropriate glioblastoma models for testing novel drugs and new treatment modalities.  
Therefore we established a novel long-term 3D glioblastoma biomimicking model system that would enable optimal drug testing in clinically more relevant duration. Glioblastoma U87 cells were immobilized in alginate microtubes and cultivated for 28 days, initially under static conditions. Cell viability, morphology and aggregate formation were monitored under fluorescent and confocal microscopes upon double staining with calcein-AM/propidium iodide. Most importantly, we investigated the effects of two different TMZ treatment modalities on cell viability and expression of resistance-related genes, MGMT and ABCB1. All treatments with 100 μm TMZ started on day 7 (X=7). We compared 3-day subsequent treatment modality (day by day treatments, X+1) with protractive treatment modality (every 7th day, X+7). 
In established 3D model system, cells managed to grow for up to 28 days without propagation, formed aggregates and constantly increased their number. Both treatment modalities had the same effects on cell viability. However, three day treatment in a row led to a tremendous increase in the expression of resistance markers, which was significantly higher compared to protractive treatment modality.
	The results showed that our 3D model system is suitable for drug testing and revealed that protractive treatment modality could be more beneficial for GBM patients.
PB  - The European Association for Cancer Research
C3  - Goodbye Flat Biology: Next Generation Cancer Models; 2021 Oct 5-6; Virtual event, Worldwide
T1  - Evaluation of different temozolomide treatment modalities in a novel long-term 3D glioblastoma cell culture
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_4904
ER  - 

@conference{
author = "Jovanović Stojanov, Sofija and Dragoj, Miodrag and Jovanović, Mirna and Stojkovska, Jasmina and Stankovic, Tijana and Dinić, Jelena and Podolski-Renić, Ana and Stepanović, Ana and Obradović, Bojana and Pešić, Milica",
year = "2021",
abstract = "Glioblastoma (GMB) is the most common and aggressive primary malignant brain tumor. Median life expectancy with the only clinically approved treatment option, Stupp protocol, is 11-15 months. This protocol involves surgical resection followed by 6 weeks of radiation and chemotherapy and then 6 cycles of 5-day chemotherapy treatment and 23 days of recovery. The only approved chemotherapeutic drug currently used is temozolomide (TMZ) that improves patients' survival for 2.5 months compared to radiotherapy alone. Such limited therapeutic options could be partly due to the lack of appropriate glioblastoma models for testing novel drugs and new treatment modalities.  
Therefore we established a novel long-term 3D glioblastoma biomimicking model system that would enable optimal drug testing in clinically more relevant duration. Glioblastoma U87 cells were immobilized in alginate microtubes and cultivated for 28 days, initially under static conditions. Cell viability, morphology and aggregate formation were monitored under fluorescent and confocal microscopes upon double staining with calcein-AM/propidium iodide. Most importantly, we investigated the effects of two different TMZ treatment modalities on cell viability and expression of resistance-related genes, MGMT and ABCB1. All treatments with 100 μm TMZ started on day 7 (X=7). We compared 3-day subsequent treatment modality (day by day treatments, X+1) with protractive treatment modality (every 7th day, X+7). 
In established 3D model system, cells managed to grow for up to 28 days without propagation, formed aggregates and constantly increased their number. Both treatment modalities had the same effects on cell viability. However, three day treatment in a row led to a tremendous increase in the expression of resistance markers, which was significantly higher compared to protractive treatment modality.
	The results showed that our 3D model system is suitable for drug testing and revealed that protractive treatment modality could be more beneficial for GBM patients.",
publisher = "The European Association for Cancer Research",
journal = "Goodbye Flat Biology: Next Generation Cancer Models; 2021 Oct 5-6; Virtual event, Worldwide",
title = "Evaluation of different temozolomide treatment modalities in a novel long-term 3D glioblastoma cell culture",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_4904"
}

Jovanović Stojanov, S., Dragoj, M., Jovanović, M., Stojkovska, J., Stankovic, T., Dinić, J., Podolski-Renić, A., Stepanović, A., Obradović, B.,& Pešić, M.. (2021). Evaluation of different temozolomide treatment modalities in a novel long-term 3D glioblastoma cell culture. in Goodbye Flat Biology: Next Generation Cancer Models; 2021 Oct 5-6; Virtual event, Worldwide
The European Association for Cancer Research..
https://hdl.handle.net/21.15107/rcub_ibiss_4904

Jovanović Stojanov S, Dragoj M, Jovanović M, Stojkovska J, Stankovic T, Dinić J, Podolski-Renić A, Stepanović A, Obradović B, Pešić M. Evaluation of different temozolomide treatment modalities in a novel long-term 3D glioblastoma cell culture. in Goodbye Flat Biology: Next Generation Cancer Models; 2021 Oct 5-6; Virtual event, Worldwide. 2021;.
https://hdl.handle.net/21.15107/rcub_ibiss_4904 .

Jovanović Stojanov, Sofija, Dragoj, Miodrag, Jovanović, Mirna, Stojkovska, Jasmina, Stankovic, Tijana, Dinić, Jelena, Podolski-Renić, Ana, Stepanović, Ana, Obradović, Bojana, Pešić, Milica, "Evaluation of different temozolomide treatment modalities in a novel long-term 3D glioblastoma cell culture" in Goodbye Flat Biology: Next Generation Cancer Models; 2021 Oct 5-6; Virtual event, Worldwide (2021),
https://hdl.handle.net/21.15107/rcub_ibiss_4904 .

TY  - JOUR
AU  - Dragoj, Miodrag
AU  - Stojkovska, Jasmina
AU  - Stanković, Tijana
AU  - Dinić, Jelena
AU  - Podolski-Renić, Ana
AU  - Obradović, Bojana
AU  - Pešić, Milica
PY  - 2021
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4294
AB  - Background: Various three-dimensional (3D) glioblastoma cell culture models have a limited duration of viability. Our aim was to develop a long-term 3D glioblastoma model, which is necessary for reliable drug response studies. Methods: Human U87 glioblastoma cells were cultured in alginate microfibers for 28 days. Cell growth, viability, morphology, and aggregation in 3D culture were monitored by fluorescent and confocal microscopy upon calcein-AM/propidium iodide (CAM/PI) staining every seven days. The glioblastoma 3D model was validated using temozolomide (TMZ) treatments 3 days in a row with a recovery period. Cell viability by MTT and resistance-related gene expression (MGMT and ABCB1) by qPCR were assessed after 28 days. The same TMZ treatment schedule was applied in 2D U87 cell culture for comparison purposes. Results: Within a long-term 3D model system in alginate fibers, U87 cells remained viable for up to 28 days. On day 7, cells formed visible aggregates oriented to the microfiber periphery. TMZ treatment reduced cell growth but increased drug resistance-related gene expression. The latter effect was more pronounced in 3D compared to 2D cell culture. Conclusion: Herein, we described a long-term glioblastoma 3D model system that could be particularly helpful for drug testing and treatment optimization.
PB  - Basel : MDPI
T2  - Brain Sciences
T1  - Development and validation of a long-term 3D glioblastoma cell culture in alginate microfibers as a novel bio-mimicking model system for preclinical drug testing
IS  - 8
VL  - 11
DO  - 10.3390/brainsci11081025
SP  - 1025
ER  - 

@article{
author = "Dragoj, Miodrag and Stojkovska, Jasmina and Stanković, Tijana and Dinić, Jelena and Podolski-Renić, Ana and Obradović, Bojana and Pešić, Milica",
year = "2021",
abstract = "Background: Various three-dimensional (3D) glioblastoma cell culture models have a limited duration of viability. Our aim was to develop a long-term 3D glioblastoma model, which is necessary for reliable drug response studies. Methods: Human U87 glioblastoma cells were cultured in alginate microfibers for 28 days. Cell growth, viability, morphology, and aggregation in 3D culture were monitored by fluorescent and confocal microscopy upon calcein-AM/propidium iodide (CAM/PI) staining every seven days. The glioblastoma 3D model was validated using temozolomide (TMZ) treatments 3 days in a row with a recovery period. Cell viability by MTT and resistance-related gene expression (MGMT and ABCB1) by qPCR were assessed after 28 days. The same TMZ treatment schedule was applied in 2D U87 cell culture for comparison purposes. Results: Within a long-term 3D model system in alginate fibers, U87 cells remained viable for up to 28 days. On day 7, cells formed visible aggregates oriented to the microfiber periphery. TMZ treatment reduced cell growth but increased drug resistance-related gene expression. The latter effect was more pronounced in 3D compared to 2D cell culture. Conclusion: Herein, we described a long-term glioblastoma 3D model system that could be particularly helpful for drug testing and treatment optimization.",
publisher = "Basel : MDPI",
journal = "Brain Sciences",
title = "Development and validation of a long-term 3D glioblastoma cell culture in alginate microfibers as a novel bio-mimicking model system for preclinical drug testing",
number = "8",
volume = "11",
doi = "10.3390/brainsci11081025",
pages = "1025"
}

Dragoj, M., Stojkovska, J., Stanković, T., Dinić, J., Podolski-Renić, A., Obradović, B.,& Pešić, M.. (2021). Development and validation of a long-term 3D glioblastoma cell culture in alginate microfibers as a novel bio-mimicking model system for preclinical drug testing. in Brain Sciences
Basel : MDPI., 11(8), 1025.
https://doi.org/10.3390/brainsci11081025

Dragoj M, Stojkovska J, Stanković T, Dinić J, Podolski-Renić A, Obradović B, Pešić M. Development and validation of a long-term 3D glioblastoma cell culture in alginate microfibers as a novel bio-mimicking model system for preclinical drug testing. in Brain Sciences. 2021;11(8):1025.
doi:10.3390/brainsci11081025 .

Dragoj, Miodrag, Stojkovska, Jasmina, Stanković, Tijana, Dinić, Jelena, Podolski-Renić, Ana, Obradović, Bojana, Pešić, Milica, "Development and validation of a long-term 3D glioblastoma cell culture in alginate microfibers as a novel bio-mimicking model system for preclinical drug testing" in Brain Sciences, 11, no. 8 (2021):1025,
https://doi.org/10.3390/brainsci11081025 . .