TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa
AU  - Borković Mitić, Slavica
AU  - Paunović, Ivan
AU  - Diklić, Aleksandar
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2019
UR  - http://link.springer.com/10.1007/s12403-019-00309-9
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3359
AB  - The background level of lead (Pb) in drinking water in Serbia represents a significant health issue. The aim of study was to examine the content of significant toxic and essential trace metals (Mn, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U) in the thyroid tissues with diagnosed colloid goiter (CG). Different variables were investigated (sex, age, smoking habits, goiter size, and previous history of any thyroid disease). The study was designed to compare the goiter tissues (GTs) to sex- and age-matched healthy- and malignant-thyroid tissues (HTTs and MTTs, respectively). All trace metals were quantified by inductively coupled plasma mass spectrometry (ICP-MS). The reduced content of essential elements (Mn and Se) and increased content of toxic metals (Pb, Th, and U) were found in GTs compared with HTTs. The increased content of the same toxic metals separated GTs from MTTs, as well as reduced content of Mn and Cd. Our results indicated that Pb acts as the main goitrogen, which could highlight its role in the unknown etiology of CG disease. Pb/Se ratio could be considered as a relevant parameter for the tissue separation of GTs from HTTs and/or MTTs. This research also provides novel data on the effects of metal pollutants on thyroid health hazards.
T2  - Exposure and Health
T1  - Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease
DO  - 10.1007/s12403-019-00309-9
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa and Borković Mitić, Slavica and Paunović, Ivan and Diklić, Aleksandar and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2019",
abstract = "The background level of lead (Pb) in drinking water in Serbia represents a significant health issue. The aim of study was to examine the content of significant toxic and essential trace metals (Mn, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U) in the thyroid tissues with diagnosed colloid goiter (CG). Different variables were investigated (sex, age, smoking habits, goiter size, and previous history of any thyroid disease). The study was designed to compare the goiter tissues (GTs) to sex- and age-matched healthy- and malignant-thyroid tissues (HTTs and MTTs, respectively). All trace metals were quantified by inductively coupled plasma mass spectrometry (ICP-MS). The reduced content of essential elements (Mn and Se) and increased content of toxic metals (Pb, Th, and U) were found in GTs compared with HTTs. The increased content of the same toxic metals separated GTs from MTTs, as well as reduced content of Mn and Cd. Our results indicated that Pb acts as the main goitrogen, which could highlight its role in the unknown etiology of CG disease. Pb/Se ratio could be considered as a relevant parameter for the tissue separation of GTs from HTTs and/or MTTs. This research also provides novel data on the effects of metal pollutants on thyroid health hazards.",
journal = "Exposure and Health",
title = "Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease",
doi = "10.1007/s12403-019-00309-9"
}

Stojsavljević, A., Rovčanin, B., Krstić, Đ., Borković Mitić, S., Paunović, I., Diklić, A., Gavrović-Jankulović, M.,& Manojlović, D.. (2019). Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease. in Exposure and Health.
https://doi.org/10.1007/s12403-019-00309-9

Stojsavljević A, Rovčanin B, Krstić Đ, Borković Mitić S, Paunović I, Diklić A, Gavrović-Jankulović M, Manojlović D. Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease. in Exposure and Health. 2019;.
doi:10.1007/s12403-019-00309-9 .

Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa, Borković Mitić, Slavica, Paunović, Ivan, Diklić, Aleksandar, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Risk Assessment of Toxic and Essential Trace Metals on the Thyroid Health at the Tissue Level: The Significance of Lead and Selenium for Colloid Goiter Disease" in Exposure and Health (2019),
https://doi.org/10.1007/s12403-019-00309-9 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa
AU  - Jagodić, Jovana
AU  - Borković Mitić, Slavica
AU  - Paunović, Ivan
AU  - Živaljević, Vladan
AU  - Mitić, Bojan
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0946672X19300070?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3435
AB  - BACKGROUND The etiology of papillary thyroid carcinoma (PTC) is unknown and some literature data support the hypothesis that heavy metals, as endocrine disrupters, could play a major role in the pathogenesis of thyroid cancer. This study aimed to estimate the content of selected toxic and essential trace metals (Mn, Co, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U), as well as the selected ratio’s (Cu/Zn and Cd/Se) in the malignant thyroid tissues according to sex, age, smoking habits, familial history of any thyroid disease, pathohistological (PH) types of PTC, tumor size, the existence of a thyroid capsular invasion, intrathyroid tumor dissemination, retrosternal thyroid growth, and TNM progress of PTC. METHODS The study included 66 patients with PTC (women/men ratio = 46/20, mean age: 54 ± 14 years). A comparative analysis was made by collecting the healthy thyroid tissues (HTTs) of the same patients, making the total number of samples 132. All trace metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). RESULTS Metals that significantly separated papillary thyroid tissues (PTTs) from the HTTs were Cd, U and Se (p < 0.05). The obtained negative correlation between Cd and Se in the PTTs could explain extrusion of essential Se caused by increased content of Cd. Only Cd had an influence on the retrosternal thyroid growth, while the essential metals (Mn, Co, and Zn) had an influence on thyroid capsular invasion. CONCLUSION It was found that Cd act as the main endocrine disrupter, which could highlight its role in the etiology of PTC. Considering that the Cd/Se ratio significantly separated two studied groups and had an influence on the retrosternal thyroid growth, its altered content could contribute to the better understanding of the molecular basis for pathophysiological changes in the PTC.
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology
VL  - 55
DO  - 10.1016/J.JTEMB.2019.06.009
SP  - 190
EP  - 195
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa and Jagodić, Jovana and Borković Mitić, Slavica and Paunović, Ivan and Živaljević, Vladan and Mitić, Bojan and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2019",
abstract = "BACKGROUND The etiology of papillary thyroid carcinoma (PTC) is unknown and some literature data support the hypothesis that heavy metals, as endocrine disrupters, could play a major role in the pathogenesis of thyroid cancer. This study aimed to estimate the content of selected toxic and essential trace metals (Mn, Co, Ni, Cu, Zn, As, Se, Cd, Pb, Th, and U), as well as the selected ratio’s (Cu/Zn and Cd/Se) in the malignant thyroid tissues according to sex, age, smoking habits, familial history of any thyroid disease, pathohistological (PH) types of PTC, tumor size, the existence of a thyroid capsular invasion, intrathyroid tumor dissemination, retrosternal thyroid growth, and TNM progress of PTC. METHODS The study included 66 patients with PTC (women/men ratio = 46/20, mean age: 54 ± 14 years). A comparative analysis was made by collecting the healthy thyroid tissues (HTTs) of the same patients, making the total number of samples 132. All trace metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). RESULTS Metals that significantly separated papillary thyroid tissues (PTTs) from the HTTs were Cd, U and Se (p < 0.05). The obtained negative correlation between Cd and Se in the PTTs could explain extrusion of essential Se caused by increased content of Cd. Only Cd had an influence on the retrosternal thyroid growth, while the essential metals (Mn, Co, and Zn) had an influence on thyroid capsular invasion. CONCLUSION It was found that Cd act as the main endocrine disrupter, which could highlight its role in the etiology of PTC. Considering that the Cd/Se ratio significantly separated two studied groups and had an influence on the retrosternal thyroid growth, its altered content could contribute to the better understanding of the molecular basis for pathophysiological changes in the PTC.",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology",
volume = "55",
doi = "10.1016/J.JTEMB.2019.06.009",
pages = "190-195"
}

Stojsavljević, A., Rovčanin, B., Krstić, Đ., Jagodić, J., Borković Mitić, S., Paunović, I., Živaljević, V., Mitić, B., Gavrović-Jankulović, M.,& Manojlović, D.. (2019). Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology. in Journal of Trace Elements in Medicine and Biology, 55, 190-195.
https://doi.org/10.1016/J.JTEMB.2019.06.009

Stojsavljević A, Rovčanin B, Krstić Đ, Jagodić J, Borković Mitić S, Paunović I, Živaljević V, Mitić B, Gavrović-Jankulović M, Manojlović D. Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology. in Journal of Trace Elements in Medicine and Biology. 2019;55:190-195.
doi:10.1016/J.JTEMB.2019.06.009 .

Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa, Jagodić, Jovana, Borković Mitić, Slavica, Paunović, Ivan, Živaljević, Vladan, Mitić, Bojan, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Cadmium as main endocrine disruptor in papillary thyroid carcinoma and the significance of Cd/Se ratio for thyroid tissue pathophysiology" in Journal of Trace Elements in Medicine and Biology, 55 (2019):190-195,
https://doi.org/10.1016/J.JTEMB.2019.06.009 . .

TY  - JOUR
AU  - Stojsavljević, Aleksandar
AU  - Rovčanin, Branislav
AU  - Krstić, Đurđa
AU  - Borković Mitić, Slavica
AU  - Paunović, Ivan
AU  - Kodranov, Igor
AU  - Gavrović-Jankulović, Marija
AU  - Manojlović, Dragan
PY  - 2019
UR  - https://www.sciencedirect.com/science/article/pii/S0147651319308103?via%3Dihub
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3450
AB  - Evaluation of trace metals at level of solid tissue can provide better information than blood or urine and, therefore, could highlight the role of metals in the etiology of organ-specific disease. The current study aimed to establish the baseline content of four essential (Mn, Cu, Zn, Se) and four toxic metals (As, Cd, Pb, U) in the healthy thyroid tissues (HTTs) by considering sex, age and smoking habits. A further aim was to examine whether differences in the content of metals exist in regard to the thyroid diseases, such as benign tumor (BT), Hashimoto's thyroiditis (HT), multinodular goiter (MNG) and thyroid cancer (TC). A total number of investigated tissue samples were 423. All metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). It was found that the content of Cu and U was higher in HTTs of women, while the content of Zn was higher in HTTs of men. Increased content of Zn and decreased content of U was found in the group of HTTs above 50 years compared to a younger group (<50 years). Increased content of Cd, Pb and U distinguish smokers from the non-smokers. In comparison with other population groups worldwide, investigated Serbian population had up to 15 times reduced content of Se. Despite the difference in metal's profile according to biological variables, this study also demonstrated, for the first time, that each thyroid disease has its unique metal's profile. The most altered metal's content was found in tissues with HT. Contrarily, the greatest similarity in metal's content with HTTs was found in BT tissues. Based on the increased content, metal's that dominantly discriminated HTTs from the HT, MNG and TC was As, Pb and Cd, respectively. Reported results could highlight the role of toxic and essential trace metals in the not very well clarified etiology of thyroid diseases and, moreover, could provide a molecular basis for pathophysiological changes of metal's hazardous effects on thyroid health at the tissue level.
T2  - Ecotoxicology and Environmental Safety
T1  - Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.
VL  - 183
DO  - 10.1016/j.ecoenv.2019.109479
SP  - 109479
ER  - 

@article{
author = "Stojsavljević, Aleksandar and Rovčanin, Branislav and Krstić, Đurđa and Borković Mitić, Slavica and Paunović, Ivan and Kodranov, Igor and Gavrović-Jankulović, Marija and Manojlović, Dragan",
year = "2019",
abstract = "Evaluation of trace metals at level of solid tissue can provide better information than blood or urine and, therefore, could highlight the role of metals in the etiology of organ-specific disease. The current study aimed to establish the baseline content of four essential (Mn, Cu, Zn, Se) and four toxic metals (As, Cd, Pb, U) in the healthy thyroid tissues (HTTs) by considering sex, age and smoking habits. A further aim was to examine whether differences in the content of metals exist in regard to the thyroid diseases, such as benign tumor (BT), Hashimoto's thyroiditis (HT), multinodular goiter (MNG) and thyroid cancer (TC). A total number of investigated tissue samples were 423. All metals were quantified by inductively coupled plasma-mass spectrometry (ICP-MS). It was found that the content of Cu and U was higher in HTTs of women, while the content of Zn was higher in HTTs of men. Increased content of Zn and decreased content of U was found in the group of HTTs above 50 years compared to a younger group (<50 years). Increased content of Cd, Pb and U distinguish smokers from the non-smokers. In comparison with other population groups worldwide, investigated Serbian population had up to 15 times reduced content of Se. Despite the difference in metal's profile according to biological variables, this study also demonstrated, for the first time, that each thyroid disease has its unique metal's profile. The most altered metal's content was found in tissues with HT. Contrarily, the greatest similarity in metal's content with HTTs was found in BT tissues. Based on the increased content, metal's that dominantly discriminated HTTs from the HT, MNG and TC was As, Pb and Cd, respectively. Reported results could highlight the role of toxic and essential trace metals in the not very well clarified etiology of thyroid diseases and, moreover, could provide a molecular basis for pathophysiological changes of metal's hazardous effects on thyroid health at the tissue level.",
journal = "Ecotoxicology and Environmental Safety",
title = "Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.",
volume = "183",
doi = "10.1016/j.ecoenv.2019.109479",
pages = "109479"
}

Stojsavljević, A., Rovčanin, B., Krstić, Đ., Borković Mitić, S., Paunović, I., Kodranov, I., Gavrović-Jankulović, M.,& Manojlović, D.. (2019). Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.. in Ecotoxicology and Environmental Safety, 183, 109479.
https://doi.org/10.1016/j.ecoenv.2019.109479

Stojsavljević A, Rovčanin B, Krstić Đ, Borković Mitić S, Paunović I, Kodranov I, Gavrović-Jankulović M, Manojlović D. Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases.. in Ecotoxicology and Environmental Safety. 2019;183:109479.
doi:10.1016/j.ecoenv.2019.109479 .

Stojsavljević, Aleksandar, Rovčanin, Branislav, Krstić, Đurđa, Borković Mitić, Slavica, Paunović, Ivan, Kodranov, Igor, Gavrović-Jankulović, Marija, Manojlović, Dragan, "Evaluation of trace metals in thyroid tissues: Comparative analysis with benign and malignant thyroid diseases." in Ecotoxicology and Environmental Safety, 183 (2019):109479,
https://doi.org/10.1016/j.ecoenv.2019.109479 . .

TY  - JOUR
AU  - Damjanović, Svetozar S
AU  - Antić, Jadranka A
AU  - Ilić, Bojana B
AU  - Beleslin-Cokić, Bojana B
AU  - Ivović, Miomira S
AU  - Ognjanović, Sanja I
AU  - Isailović, Tatjana V
AU  - Popović, Bojana M
AU  - Bozić, Ivana B
AU  - Tatić, Svetislav B
AU  - Matić, Gordana
AU  - Todorović, Vera N
AU  - Paunović, Ivan R
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1085
AB  - Glucocorticoid (GC) sensitivity depends on glucocorticoid receptor (GR) and heat shock proteins (Hsps). We investigated whether common GR genes (ER22/23EK N363S, BclI, and 9 beta) and adrenocorticotropin receptor promoter polymorphisms influence susceptibility for unilateral adrenal incidentaloma (AI), plus GR and Hsp expression in tumorous (n = 19), peritumorous (n = 13) and normal adrenocortical (n = 11) tissues. Patients (n = 112), population-matched controls (n = 100) and tumor tissues (n = 32) were genotyped for these polymorphisms. Postdexamethasone serum cortisol was higher in patients (p<0.001). GR gene variants, larger allele of BclI (odds ratio (OR) 2.9; 95% confidence interval (CI) 1.7-5.1; p < 0.001) and minor allele of 9 beta (OR 3.0; 95% CI 1.6-5.7; p < 0.001) were independent predictors of Al. In patients, the first allele is linked with larger tumors (p = 0.002) and the latter with higher postdexamethasone cortisol levels (p = 0.025). Both allele carriers had lesser waist circumference (p = 0.02), similar adrenocorticotropin and higher basal (p = 0.024) and postdexamethasone cortisol concentrations (p < 0.001). Tumorous and constitutional genotypes were similar. GR-D is the major receptor isoform in normal adrenal cortex by Western blotting. Loss of other receptor isoforms, decrease in immunostaining for GR (p < 0.0001), underexpression of chaperones (p <= 0.01) and the presence of inducible Hsp70 were found in adenomas. In conclusion, GR gene variants, C allele of BclI and minor allele of 9 beta, are associated with Als. Their concurrent presence in patients reduces GC sensitivity Normal adrenal cortex preferentially expresses GR-D. In adenomas, the lack of other GR isoforms and underexpression of heat shock proteins perhaps permanently impair GC signaling, which could promote dysregulated cortisol production and tumor growth. The innate GC sensitivity probably modifies these effects. Online address: http://www.molmed.org doi: 10.2119/molmed.2012.00261
T2  - Molecular Medicine
T1  - Glucocorticoid Receptor and Molecular Chaperones in the Pathogenesis of Adrenal Incidentalomas: Potential Role of Reduced Sensitivity to Glucocorticoids
IS  - 11
VL  - 18
SP  - 189
EP  - 1465
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1085
ER  - 

@article{
author = "Damjanović, Svetozar S and Antić, Jadranka A and Ilić, Bojana B and Beleslin-Cokić, Bojana B and Ivović, Miomira S and Ognjanović, Sanja I and Isailović, Tatjana V and Popović, Bojana M and Bozić, Ivana B and Tatić, Svetislav B and Matić, Gordana and Todorović, Vera N and Paunović, Ivan R",
year = "2012",
abstract = "Glucocorticoid (GC) sensitivity depends on glucocorticoid receptor (GR) and heat shock proteins (Hsps). We investigated whether common GR genes (ER22/23EK N363S, BclI, and 9 beta) and adrenocorticotropin receptor promoter polymorphisms influence susceptibility for unilateral adrenal incidentaloma (AI), plus GR and Hsp expression in tumorous (n = 19), peritumorous (n = 13) and normal adrenocortical (n = 11) tissues. Patients (n = 112), population-matched controls (n = 100) and tumor tissues (n = 32) were genotyped for these polymorphisms. Postdexamethasone serum cortisol was higher in patients (p<0.001). GR gene variants, larger allele of BclI (odds ratio (OR) 2.9; 95% confidence interval (CI) 1.7-5.1; p < 0.001) and minor allele of 9 beta (OR 3.0; 95% CI 1.6-5.7; p < 0.001) were independent predictors of Al. In patients, the first allele is linked with larger tumors (p = 0.002) and the latter with higher postdexamethasone cortisol levels (p = 0.025). Both allele carriers had lesser waist circumference (p = 0.02), similar adrenocorticotropin and higher basal (p = 0.024) and postdexamethasone cortisol concentrations (p < 0.001). Tumorous and constitutional genotypes were similar. GR-D is the major receptor isoform in normal adrenal cortex by Western blotting. Loss of other receptor isoforms, decrease in immunostaining for GR (p < 0.0001), underexpression of chaperones (p <= 0.01) and the presence of inducible Hsp70 were found in adenomas. In conclusion, GR gene variants, C allele of BclI and minor allele of 9 beta, are associated with Als. Their concurrent presence in patients reduces GC sensitivity Normal adrenal cortex preferentially expresses GR-D. In adenomas, the lack of other GR isoforms and underexpression of heat shock proteins perhaps permanently impair GC signaling, which could promote dysregulated cortisol production and tumor growth. The innate GC sensitivity probably modifies these effects. Online address: http://www.molmed.org doi: 10.2119/molmed.2012.00261",
journal = "Molecular Medicine",
title = "Glucocorticoid Receptor and Molecular Chaperones in the Pathogenesis of Adrenal Incidentalomas: Potential Role of Reduced Sensitivity to Glucocorticoids",
number = "11",
volume = "18",
pages = "189-1465",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1085"
}

Damjanović, S. S., Antić, J. A., Ilić, B. B., Beleslin-Cokić, B. B., Ivović, M. S., Ognjanović, S. I., Isailović, T. V., Popović, B. M., Bozić, I. B., Tatić, S. B., Matić, G., Todorović, V. N.,& Paunović, I. R.. (2012). Glucocorticoid Receptor and Molecular Chaperones in the Pathogenesis of Adrenal Incidentalomas: Potential Role of Reduced Sensitivity to Glucocorticoids. in Molecular Medicine, 18(11), 189-1465.
https://hdl.handle.net/21.15107/rcub_ibiss_1085

Damjanović SS, Antić JA, Ilić BB, Beleslin-Cokić BB, Ivović MS, Ognjanović SI, Isailović TV, Popović BM, Bozić IB, Tatić SB, Matić G, Todorović VN, Paunović IR. Glucocorticoid Receptor and Molecular Chaperones in the Pathogenesis of Adrenal Incidentalomas: Potential Role of Reduced Sensitivity to Glucocorticoids. in Molecular Medicine. 2012;18(11):189-1465.
https://hdl.handle.net/21.15107/rcub_ibiss_1085 .

Damjanović, Svetozar S, Antić, Jadranka A, Ilić, Bojana B, Beleslin-Cokić, Bojana B, Ivović, Miomira S, Ognjanović, Sanja I, Isailović, Tatjana V, Popović, Bojana M, Bozić, Ivana B, Tatić, Svetislav B, Matić, Gordana, Todorović, Vera N, Paunović, Ivan R, "Glucocorticoid Receptor and Molecular Chaperones in the Pathogenesis of Adrenal Incidentalomas: Potential Role of Reduced Sensitivity to Glucocorticoids" in Molecular Medicine, 18, no. 11 (2012):189-1465,
https://hdl.handle.net/21.15107/rcub_ibiss_1085 .