TY  - JOUR
AU  - Kaluđerović, Goran
AU  - Bulatović, Mirna
AU  - Krajnović, Tamara
AU  - Paschke, Reinhard
AU  - B. Zmejkovski, Bojana
AU  - Maksimović-Ivanić, Danijela
AU  - Mijatović, Sanja
PY  - 2017
UR  - http://www.mdpi.com/2304-6740/5/3/56
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3042
AB  - Synthesis of platinum(II) conjugate with acetylated betulinic acid tris(hydroxymethyl)aminomethane ester (BATRIS) is presented (BATRISPt). HR-ESI-MS and multinuclear NMR spectroscopy, as well as elemental analysis were used for characterization of BATRISPt. Cytotoxicity (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), crystal violet (CV), and sulforhodamine B (SRB) assays) of BA, BATRIS, BATRISPt, and cisplatin were assessed on seven different tumor cell lines: melanoma B16, colon HCT116 and DLD-1, adenocarcinoma HeLa, breast MCF-7, and anaplastic thyroid tumor 8505C and SW1736; as well as normal MRC-5 fibroblasts. Furthermore, the effect of the mentioned compounds on the apoptosis (Annexin V/PI assay) and autophagy induction (acridine orange (AO) assay) as well as caspase 3, 8, and 9 activation were investigated on the selected B16 melanoma cell line. BATRISPt showed lower activity than BA, BATRIS, or cisplatin. All tested compounds triggered apoptosis in B16 cells. Induction of autophagy was observed in B16 cells exposed only to BATRIS. On the other hand, new conjugate activates caspases 8 and 9 in B16 cells with higher impact than BATRIS or cisplatin alone.
T2  - Inorganics
T1  - (18-Crown-6)potassium(I) Trichlorido[28-acetyl-3-(tris-(hydroxylmethyl)amino-ethane)betulinic ester-κN]platinum(II): Synthesis and In Vitro Antitumor Activity
IS  - 3
VL  - 5
DO  - 10.3390/inorganics5030056
SP  - 56
ER  - 

@article{
author = "Kaluđerović, Goran and Bulatović, Mirna and Krajnović, Tamara and Paschke, Reinhard and B. Zmejkovski, Bojana and Maksimović-Ivanić, Danijela and Mijatović, Sanja",
year = "2017",
abstract = "Synthesis of platinum(II) conjugate with acetylated betulinic acid tris(hydroxymethyl)aminomethane ester (BATRIS) is presented (BATRISPt). HR-ESI-MS and multinuclear NMR spectroscopy, as well as elemental analysis were used for characterization of BATRISPt. Cytotoxicity (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), crystal violet (CV), and sulforhodamine B (SRB) assays) of BA, BATRIS, BATRISPt, and cisplatin were assessed on seven different tumor cell lines: melanoma B16, colon HCT116 and DLD-1, adenocarcinoma HeLa, breast MCF-7, and anaplastic thyroid tumor 8505C and SW1736; as well as normal MRC-5 fibroblasts. Furthermore, the effect of the mentioned compounds on the apoptosis (Annexin V/PI assay) and autophagy induction (acridine orange (AO) assay) as well as caspase 3, 8, and 9 activation were investigated on the selected B16 melanoma cell line. BATRISPt showed lower activity than BA, BATRIS, or cisplatin. All tested compounds triggered apoptosis in B16 cells. Induction of autophagy was observed in B16 cells exposed only to BATRIS. On the other hand, new conjugate activates caspases 8 and 9 in B16 cells with higher impact than BATRIS or cisplatin alone.",
journal = "Inorganics",
title = "(18-Crown-6)potassium(I) Trichlorido[28-acetyl-3-(tris-(hydroxylmethyl)amino-ethane)betulinic ester-κN]platinum(II): Synthesis and In Vitro Antitumor Activity",
number = "3",
volume = "5",
doi = "10.3390/inorganics5030056",
pages = "56"
}

Kaluđerović, G., Bulatović, M., Krajnović, T., Paschke, R., B. Zmejkovski, B., Maksimović-Ivanić, D.,& Mijatović, S.. (2017). (18-Crown-6)potassium(I) Trichlorido[28-acetyl-3-(tris-(hydroxylmethyl)amino-ethane)betulinic ester-κN]platinum(II): Synthesis and In Vitro Antitumor Activity. in Inorganics, 5(3), 56.
https://doi.org/10.3390/inorganics5030056

Kaluđerović G, Bulatović M, Krajnović T, Paschke R, B. Zmejkovski B, Maksimović-Ivanić D, Mijatović S. (18-Crown-6)potassium(I) Trichlorido[28-acetyl-3-(tris-(hydroxylmethyl)amino-ethane)betulinic ester-κN]platinum(II): Synthesis and In Vitro Antitumor Activity. in Inorganics. 2017;5(3):56.
doi:10.3390/inorganics5030056 .

Kaluđerović, Goran, Bulatović, Mirna, Krajnović, Tamara, Paschke, Reinhard, B. Zmejkovski, Bojana, Maksimović-Ivanić, Danijela, Mijatović, Sanja, "(18-Crown-6)potassium(I) Trichlorido[28-acetyl-3-(tris-(hydroxylmethyl)amino-ethane)betulinic ester-κN]platinum(II): Synthesis and In Vitro Antitumor Activity" in Inorganics, 5, no. 3 (2017):56,
https://doi.org/10.3390/inorganics5030056 . .

TY  - JOUR
AU  - Blaževski, Jana
AU  - Petković, Filip
AU  - Momčilović, Miljana
AU  - Paschke, Reinhard
AU  - Kaluđerović, Goran N.
AU  - Mostarica-Stojković, Marija B
AU  - Miljković, Đorđe
PY  - 2013
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1037
AB  - Aim: To investigate the influences of betulinic acid (BA), a triterpenoid isolated from birch bark, on neuroinflammatory mediators involved in the pathogenesis of multiple sclerosis and experimental autoimmune encephalomyelitis in vitro. Methods: Encephalitogenic T cells were prepared from draining lymph nodes and spinal cords of Dark Agouti rats 8 to 10 d after immunization with myelin basic protein (MBP) and complete Freund's adjuvant. Macrophages were isolated from the peritoneal cavity of adult untreated rats. Astrocytes were isolated from neonatal rat brains. The cells were cultured and then treated with different agents. IFN-gamma, IL-17, iNOS and CXCL12 mRNA levels in the cells were analyzed with RT-PCR. iNOS and CXCL12 protein levels were detected using immunoblot. NO and ROS generation was measured using Griess reaction and flow cytometry, respectively. Results: In encephalitogenic T cells stimulated with MBP (10 mu g/mL), addition of BA inhibited IL-17 and IFN-gamma production in a dose-dependent manner. The estimated IC50 values for IL-17 and IFN gamma were 11.2 and 63.8 mu mol/L, respectively. When the macrophages were stimulated with LPS (10 ng/mL), addition of BA (50 mu mol/L) significantly increased ROS generation, and suppressed NO generation. The astrocytes were stimulated with ConASn containing numerous inflammatory mediators, which mimicked the inflammatory milieu within CNS; addition of BA (50 mu mol/L) significantly increased ROS generation, and blocked ConASn-induced increases in iNOS and CXCL12 mRNA levels, but did not affect iNOS and CXCL12 protein levels. Importantly, in both the macrophages and astrocytes, addition of BA (50 mu mol/L) inhibited lipid peroxidation. Conclusion: Besides inhibiting encephalitogenic T cell cytokines and reducing NO generation, BA induces tissue-damaging ROS generation within CNS.
T2  - Acta Pharmacologica Sinica
T1  - Betulinic acid regulates generation of neuroinflammatory mediators responsible for tissue destruction in multiple sclerosis in vitro
IS  - 3
VL  - 34
SP  - 51
EP  - 431
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1037
ER  - 

@article{
author = "Blaževski, Jana and Petković, Filip and Momčilović, Miljana and Paschke, Reinhard and Kaluđerović, Goran N. and Mostarica-Stojković, Marija B and Miljković, Đorđe",
year = "2013",
abstract = "Aim: To investigate the influences of betulinic acid (BA), a triterpenoid isolated from birch bark, on neuroinflammatory mediators involved in the pathogenesis of multiple sclerosis and experimental autoimmune encephalomyelitis in vitro. Methods: Encephalitogenic T cells were prepared from draining lymph nodes and spinal cords of Dark Agouti rats 8 to 10 d after immunization with myelin basic protein (MBP) and complete Freund's adjuvant. Macrophages were isolated from the peritoneal cavity of adult untreated rats. Astrocytes were isolated from neonatal rat brains. The cells were cultured and then treated with different agents. IFN-gamma, IL-17, iNOS and CXCL12 mRNA levels in the cells were analyzed with RT-PCR. iNOS and CXCL12 protein levels were detected using immunoblot. NO and ROS generation was measured using Griess reaction and flow cytometry, respectively. Results: In encephalitogenic T cells stimulated with MBP (10 mu g/mL), addition of BA inhibited IL-17 and IFN-gamma production in a dose-dependent manner. The estimated IC50 values for IL-17 and IFN gamma were 11.2 and 63.8 mu mol/L, respectively. When the macrophages were stimulated with LPS (10 ng/mL), addition of BA (50 mu mol/L) significantly increased ROS generation, and suppressed NO generation. The astrocytes were stimulated with ConASn containing numerous inflammatory mediators, which mimicked the inflammatory milieu within CNS; addition of BA (50 mu mol/L) significantly increased ROS generation, and blocked ConASn-induced increases in iNOS and CXCL12 mRNA levels, but did not affect iNOS and CXCL12 protein levels. Importantly, in both the macrophages and astrocytes, addition of BA (50 mu mol/L) inhibited lipid peroxidation. Conclusion: Besides inhibiting encephalitogenic T cell cytokines and reducing NO generation, BA induces tissue-damaging ROS generation within CNS.",
journal = "Acta Pharmacologica Sinica",
title = "Betulinic acid regulates generation of neuroinflammatory mediators responsible for tissue destruction in multiple sclerosis in vitro",
number = "3",
volume = "34",
pages = "51-431",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1037"
}

Blaževski, J., Petković, F., Momčilović, M., Paschke, R., Kaluđerović, G. N., Mostarica-Stojković, M. B.,& Miljković, Đ.. (2013). Betulinic acid regulates generation of neuroinflammatory mediators responsible for tissue destruction in multiple sclerosis in vitro. in Acta Pharmacologica Sinica, 34(3), 51-431.
https://hdl.handle.net/21.15107/rcub_ibiss_1037

Blaževski J, Petković F, Momčilović M, Paschke R, Kaluđerović GN, Mostarica-Stojković MB, Miljković Đ. Betulinic acid regulates generation of neuroinflammatory mediators responsible for tissue destruction in multiple sclerosis in vitro. in Acta Pharmacologica Sinica. 2013;34(3):51-431.
https://hdl.handle.net/21.15107/rcub_ibiss_1037 .

Blaževski, Jana, Petković, Filip, Momčilović, Miljana, Paschke, Reinhard, Kaluđerović, Goran N., Mostarica-Stojković, Marija B, Miljković, Đorđe, "Betulinic acid regulates generation of neuroinflammatory mediators responsible for tissue destruction in multiple sclerosis in vitro" in Acta Pharmacologica Sinica, 34, no. 3 (2013):51-431,
https://hdl.handle.net/21.15107/rcub_ibiss_1037 .

TY  - GEN
AU  - Kaluđerović, Goran N.
AU  - Gomez-Ruiz, Santiago
AU  - Maksimović-Ivanić, Danijela
AU  - Paschke, Reinhard
AU  - Mijatović, Sanja
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1225
T2  - Bioinorganic Chemistry and Applications
T1  - Metals in Medicine
IS  - null
VL  - null
EP  - na
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1225
ER  - 

@misc{
author = "Kaluđerović, Goran N. and Gomez-Ruiz, Santiago and Maksimović-Ivanić, Danijela and Paschke, Reinhard and Mijatović, Sanja",
year = "2012",
journal = "Bioinorganic Chemistry and Applications",
title = "Metals in Medicine",
number = "null",
volume = "null",
pages = "na",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1225"
}

Kaluđerović, G. N., Gomez-Ruiz, S., Maksimović-Ivanić, D., Paschke, R.,& Mijatović, S.. (2012). Metals in Medicine. in Bioinorganic Chemistry and Applications, null(null).
https://hdl.handle.net/21.15107/rcub_ibiss_1225

Kaluđerović GN, Gomez-Ruiz S, Maksimović-Ivanić D, Paschke R, Mijatović S. Metals in Medicine. in Bioinorganic Chemistry and Applications. 2012;null(null):null-na.
https://hdl.handle.net/21.15107/rcub_ibiss_1225 .

Kaluđerović, Goran N., Gomez-Ruiz, Santiago, Maksimović-Ivanić, Danijela, Paschke, Reinhard, Mijatović, Sanja, "Metals in Medicine" in Bioinorganic Chemistry and Applications, null, no. null (2012),
https://hdl.handle.net/21.15107/rcub_ibiss_1225 .