TY  - JOUR
AU  - Tanić, Nikola T
AU  - Milovanović, Zorka
AU  - Tanić, Nasta
AU  - Džodić, Radan R.
AU  - Juranić, Zorica D
AU  - Susnjar, Snezana
AU  - Plesinac-Karapandžić, Vesna
AU  - Tatić, Svetislav B
AU  - Dramicanin, Tatjana
AU  - Davidović, Radoslav
AU  - Dimitrijević, Bogomir B.
PY  - 2012
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1100
AB  - Tamoxifen is a standard therapeutical treatment in patients with estrogen receptor positive breast carcinoma. However, less than 50% of estrogen receptor positive breast cancers do not respond to tamoxifen treatment whereas 40% of tumors that initially respond to treatment develop resistance over time. The underlying mechanisms for tamoxifen resistance are probably multifactorial but remain largely unknown. The primary aim of this study was to investigate the impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen by analyzing loss of heterozygosity (LOH) and immunohystochemical expression of PTEN in 49 primary breast carcinomas of patients treated with tamoxifen as the only adjuvant therapy. The effect of PTEN inactivation on breast cancer progression and disease outcome was also analyzed. Reduced or completely lost PTEN expression was observed in 55.1% of samples, while 63.3% of samples displayed LOH of PTEN gene. Inactivation of PTEN immunoexpression significantly correlated with the PTEN loss of heterozygosity, suggesting LOH as the most important genetic mechanism for the reduction or complete loss of PTEN expression in primary breast carcinoma. Most importantly, LOH of PTEN and consequential reduction of its immunoexpression showed significant correlation with the recurrence of the disease. Besides, our study revealed that LOH of PTEN tumor suppressor was significantly associated with shorter disease free survival, breast cancer specific survival and overall survival. In summary, our results imply that LOH of PTEN could be used as a good prognostic characteristic for the outcome of breast cancer patients treated with tamoxifen.
T2  - Cancer Biology & Therapy
T1  - The impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen treatment in breast cancer patients
IS  - 12
VL  - 13
SP  - 55
EP  - 1174
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1100
ER  - 

@article{
author = "Tanić, Nikola T and Milovanović, Zorka and Tanić, Nasta and Džodić, Radan R. and Juranić, Zorica D and Susnjar, Snezana and Plesinac-Karapandžić, Vesna and Tatić, Svetislav B and Dramicanin, Tatjana and Davidović, Radoslav and Dimitrijević, Bogomir B.",
year = "2012",
abstract = "Tamoxifen is a standard therapeutical treatment in patients with estrogen receptor positive breast carcinoma. However, less than 50% of estrogen receptor positive breast cancers do not respond to tamoxifen treatment whereas 40% of tumors that initially respond to treatment develop resistance over time. The underlying mechanisms for tamoxifen resistance are probably multifactorial but remain largely unknown. The primary aim of this study was to investigate the impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen by analyzing loss of heterozygosity (LOH) and immunohystochemical expression of PTEN in 49 primary breast carcinomas of patients treated with tamoxifen as the only adjuvant therapy. The effect of PTEN inactivation on breast cancer progression and disease outcome was also analyzed. Reduced or completely lost PTEN expression was observed in 55.1% of samples, while 63.3% of samples displayed LOH of PTEN gene. Inactivation of PTEN immunoexpression significantly correlated with the PTEN loss of heterozygosity, suggesting LOH as the most important genetic mechanism for the reduction or complete loss of PTEN expression in primary breast carcinoma. Most importantly, LOH of PTEN and consequential reduction of its immunoexpression showed significant correlation with the recurrence of the disease. Besides, our study revealed that LOH of PTEN tumor suppressor was significantly associated with shorter disease free survival, breast cancer specific survival and overall survival. In summary, our results imply that LOH of PTEN could be used as a good prognostic characteristic for the outcome of breast cancer patients treated with tamoxifen.",
journal = "Cancer Biology & Therapy",
title = "The impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen treatment in breast cancer patients",
number = "12",
volume = "13",
pages = "55-1174",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1100"
}

Tanić, N. T., Milovanović, Z., Tanić, N., Džodić, R. R., Juranić, Z. D., Susnjar, S., Plesinac-Karapandžić, V., Tatić, S. B., Dramicanin, T., Davidović, R.,& Dimitrijević, B. B.. (2012). The impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen treatment in breast cancer patients. in Cancer Biology & Therapy, 13(12), 55-1174.
https://hdl.handle.net/21.15107/rcub_ibiss_1100

Tanić NT, Milovanović Z, Tanić N, Džodić RR, Juranić ZD, Susnjar S, Plesinac-Karapandžić V, Tatić SB, Dramicanin T, Davidović R, Dimitrijević BB. The impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen treatment in breast cancer patients. in Cancer Biology & Therapy. 2012;13(12):55-1174.
https://hdl.handle.net/21.15107/rcub_ibiss_1100 .

Tanić, Nikola T, Milovanović, Zorka, Tanić, Nasta, Džodić, Radan R., Juranić, Zorica D, Susnjar, Snezana, Plesinac-Karapandžić, Vesna, Tatić, Svetislav B, Dramicanin, Tatjana, Davidović, Radoslav, Dimitrijević, Bogomir B., "The impact of PTEN tumor suppressor gene on acquiring resistance to tamoxifen treatment in breast cancer patients" in Cancer Biology & Therapy, 13, no. 12 (2012):55-1174,
https://hdl.handle.net/21.15107/rcub_ibiss_1100 .

TY  - JOUR
AU  - Tanić, Nikola T
AU  - Stanojević, Boban R
AU  - Tanić, Nasta
AU  - Schaefer, Stephan
AU  - Niesters, Hubert GM
AU  - Bozić, Milena
AU  - Dimitrijević, Bogomir B.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1424
AB  - Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.
T2  - Journal of Virological Methods
T1  - Concurrent quantitation of the A and D genotypes of hepatitis B virus
IS  - 2
VL  - 161
EP  - 270
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1424
ER  - 

@article{
author = "Tanić, Nikola T and Stanojević, Boban R and Tanić, Nasta and Schaefer, Stephan and Niesters, Hubert GM and Bozić, Milena and Dimitrijević, Bogomir B.",
year = "2009",
abstract = "Hepatitis B virus (HBV) infection is a global health problem associated with severe liver disorders. Viral load and HBV genotype affect the clinical outcome, guide antiviral therapy and provide long term prognosis for HBV infected patients. Various types of detection and quantitation assays are currently in use with a different effectiveness. The aim of this study was to develop a method that would provide simultaneous identification and quantitation of genotypes A and D in a single-tube reaction. Sera from infected patients were analyzed by a TaqMan based real time PCR. Optimized reagents were used for HBV DNA quantitation while the genotypes A and D were quantified separately by our design of the assay. Multiplex real time PCR was achieved and was specific for HBV genotypes A and D within a single-tube reaction. Simulation of mixed virus populations was identified reproducibly in vitro. Quantitation of these individual genotypes was exceptionally reliable, so much so that the sum of individual genotypes was equal to the total viral load determined in a separate reaction. Therefore, a straightforward, conceptually simple and reliable approach to issues involving HBV genotypes A and D is submitted. Identity and exact titer of these genotypes in the Caucasian population can now be determined easily. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "Journal of Virological Methods",
title = "Concurrent quantitation of the A and D genotypes of hepatitis B virus",
number = "2",
volume = "161",
pages = "270",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1424"
}

Tanić, N. T., Stanojević, B. R., Tanić, N., Schaefer, S., Niesters, H. G., Bozić, M.,& Dimitrijević, B. B.. (2009). Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods, 161(2).
https://hdl.handle.net/21.15107/rcub_ibiss_1424

Tanić NT, Stanojević BR, Tanić N, Schaefer S, Niesters HG, Bozić M, Dimitrijević BB. Concurrent quantitation of the A and D genotypes of hepatitis B virus. in Journal of Virological Methods. 2009;161(2):null-270.
https://hdl.handle.net/21.15107/rcub_ibiss_1424 .

Tanić, Nikola T, Stanojević, Boban R, Tanić, Nasta, Schaefer, Stephan, Niesters, Hubert GM, Bozić, Milena, Dimitrijević, Bogomir B., "Concurrent quantitation of the A and D genotypes of hepatitis B virus" in Journal of Virological Methods, 161, no. 2 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1424 .

TY  - JOUR
AU  - Tanić, Nasta
AU  - Tanić, Nikola T
AU  - Milasin, Jelena M
AU  - Vukadinović, Miroslav
AU  - Dimitrijević, Bogomir B.
PY  - 2009
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1448
AB  - Leukoplakias, clinically identifiable premalignant lesions, often precede oral squamous cell carcinoma (OSCC). Identification of leukoplakias that have the potential for transformation to malignancy is a key clinical problem. The aim of this study was to assess genomic instability, and to detect tumor-specific genomic alterations, in leukoplakias. Genomic instability was analyzed by comparing the DNA fingerprints of 32 leukoplakias with those of paired normal tissue. In addition, the mutational status of the p53 gene was analyzed using polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex DNA (PCR-HET), and the mutations were subsequently confirmed by DNA sequencing. Moderate-to-significant genomic instability was detected in all leukoplakias analysed. Nine unique amplicons, present in leukoplakias but not in normal tissue, were retrieved and successfully characterized. The p53 gene was mutated in 40.6% of patients. Four patients with moderate instability and mutated p53 developed OSCC. The data obtained in this study support and concretize the thesis that premalignant lesions possess many of the alterations found in cancer before the development of a malignant phenotype. Inactivation or mutation of the p53 tumor-suppressor might be an early event contributing to genomic instability and increasing the risk of malignant transformation.
T2  - European Journal of Oral Sciences
T1  - Genomic instability and tumor-specific DNA alterations in oral leukoplakias
IS  - 3
VL  - 117
EP  - 237
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1448
ER  - 

@article{
author = "Tanić, Nasta and Tanić, Nikola T and Milasin, Jelena M and Vukadinović, Miroslav and Dimitrijević, Bogomir B.",
year = "2009",
abstract = "Leukoplakias, clinically identifiable premalignant lesions, often precede oral squamous cell carcinoma (OSCC). Identification of leukoplakias that have the potential for transformation to malignancy is a key clinical problem. The aim of this study was to assess genomic instability, and to detect tumor-specific genomic alterations, in leukoplakias. Genomic instability was analyzed by comparing the DNA fingerprints of 32 leukoplakias with those of paired normal tissue. In addition, the mutational status of the p53 gene was analyzed using polymerase chain reaction-single-stranded conformational polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex DNA (PCR-HET), and the mutations were subsequently confirmed by DNA sequencing. Moderate-to-significant genomic instability was detected in all leukoplakias analysed. Nine unique amplicons, present in leukoplakias but not in normal tissue, were retrieved and successfully characterized. The p53 gene was mutated in 40.6% of patients. Four patients with moderate instability and mutated p53 developed OSCC. The data obtained in this study support and concretize the thesis that premalignant lesions possess many of the alterations found in cancer before the development of a malignant phenotype. Inactivation or mutation of the p53 tumor-suppressor might be an early event contributing to genomic instability and increasing the risk of malignant transformation.",
journal = "European Journal of Oral Sciences",
title = "Genomic instability and tumor-specific DNA alterations in oral leukoplakias",
number = "3",
volume = "117",
pages = "237",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1448"
}

Tanić, N., Tanić, N. T., Milasin, J. M., Vukadinović, M.,& Dimitrijević, B. B.. (2009). Genomic instability and tumor-specific DNA alterations in oral leukoplakias. in European Journal of Oral Sciences, 117(3).
https://hdl.handle.net/21.15107/rcub_ibiss_1448

Tanić N, Tanić NT, Milasin JM, Vukadinović M, Dimitrijević BB. Genomic instability and tumor-specific DNA alterations in oral leukoplakias. in European Journal of Oral Sciences. 2009;117(3):null-237.
https://hdl.handle.net/21.15107/rcub_ibiss_1448 .

Tanić, Nasta, Tanić, Nikola T, Milasin, Jelena M, Vukadinović, Miroslav, Dimitrijević, Bogomir B., "Genomic instability and tumor-specific DNA alterations in oral leukoplakias" in European Journal of Oral Sciences, 117, no. 3 (2009),
https://hdl.handle.net/21.15107/rcub_ibiss_1448 .

TY  - JOUR
AU  - Mandušić, Vesna
AU  - Nikolić-Vukosavljević, Dragica
AU  - Tanić, Nikola T
AU  - Kanjer, Ksenija
AU  - Nešković-Konstantinović, Zora B.
AU  - Celeketić, Dusica C
AU  - Dimitrijević, Bogomir B.
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1586
AB  - Purpose In addition to Estrogen Receptor alpha (ER alpha) and Progesterone Receptor (PR), the Second Estrogen Receptor (ER beta) appears to play an important role not only in estrogen signaling, but also in the pathogenesis of cancer in estrogen dependent tissues. The existence of various isoforms and splice variants of both ERs additionally complicates elucidation of their physiological role and involvement in the process of carcinogenesis. Methods In this study, the expression of ER beta 1 mRNA (wild type of beta receptor) and splice variant ER beta Delta 5 mRNA (which codes for truncated protein) was measured by the quantitative RT-PCR (q RT-PCR) in the 60 samples of Breast Cancer (BC) and correlated with ER alpha and PR protein levels and with clinical and histopathological parameters. Results We found the inverse correlation of ER beta Delta 5 mRNA expression with the levels of PR and ER alpha proteins in the group of postmenopausal patients; we also report the lower expression of ER beta 1 and ER beta Delta 5 mRNA in the larger tumors (>20 mm, T2, and T3) than in smaller ones (<= 20 mm, T1). The decrease of ER beta Delta 5 mRNA expression in larger tumors is found to arise from ER-positive breast carcinomas. In addition, the portion of tumors with concomitant high expression of both transcripts matches up the known percentage of tumors resistant to endocrine therapy in patients with different ER/PR status. Conclusions As far as we know, this is the first study in which ER beta Delta 5 mRNA splice variant was quantified by realtime RT-PCR in the clinical samples of breast cancer tissue. Until now, the focus of clinical reports was the level of ER beta 1, ER beta 2, and ER beta 5 isoforms. The higher expression of ER beta Delta 5 mRNA is associated with the indicators of low biological aggressiveness of tumor (low tumor size within ER-positive status in our study) suggesting that the uncontrolled local tumor growth may occur as the expression of ER beta Delta 5 mRNA decreases in estrogen-dependent breast cancer.
T2  - Journal of Cancer Research and Clinical Oncology
T1  - Expression of estrogen receptor beta wt isoform (ER beta 1) and ER beta Delta 5 splice variant mRNAs in sporadic breast cancer
IS  - 8
VL  - 133
EP  - 579
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1586
ER  - 

@article{
author = "Mandušić, Vesna and Nikolić-Vukosavljević, Dragica and Tanić, Nikola T and Kanjer, Ksenija and Nešković-Konstantinović, Zora B. and Celeketić, Dusica C and Dimitrijević, Bogomir B.",
year = "2007",
abstract = "Purpose In addition to Estrogen Receptor alpha (ER alpha) and Progesterone Receptor (PR), the Second Estrogen Receptor (ER beta) appears to play an important role not only in estrogen signaling, but also in the pathogenesis of cancer in estrogen dependent tissues. The existence of various isoforms and splice variants of both ERs additionally complicates elucidation of their physiological role and involvement in the process of carcinogenesis. Methods In this study, the expression of ER beta 1 mRNA (wild type of beta receptor) and splice variant ER beta Delta 5 mRNA (which codes for truncated protein) was measured by the quantitative RT-PCR (q RT-PCR) in the 60 samples of Breast Cancer (BC) and correlated with ER alpha and PR protein levels and with clinical and histopathological parameters. Results We found the inverse correlation of ER beta Delta 5 mRNA expression with the levels of PR and ER alpha proteins in the group of postmenopausal patients; we also report the lower expression of ER beta 1 and ER beta Delta 5 mRNA in the larger tumors (>20 mm, T2, and T3) than in smaller ones (<= 20 mm, T1). The decrease of ER beta Delta 5 mRNA expression in larger tumors is found to arise from ER-positive breast carcinomas. In addition, the portion of tumors with concomitant high expression of both transcripts matches up the known percentage of tumors resistant to endocrine therapy in patients with different ER/PR status. Conclusions As far as we know, this is the first study in which ER beta Delta 5 mRNA splice variant was quantified by realtime RT-PCR in the clinical samples of breast cancer tissue. Until now, the focus of clinical reports was the level of ER beta 1, ER beta 2, and ER beta 5 isoforms. The higher expression of ER beta Delta 5 mRNA is associated with the indicators of low biological aggressiveness of tumor (low tumor size within ER-positive status in our study) suggesting that the uncontrolled local tumor growth may occur as the expression of ER beta Delta 5 mRNA decreases in estrogen-dependent breast cancer.",
journal = "Journal of Cancer Research and Clinical Oncology",
title = "Expression of estrogen receptor beta wt isoform (ER beta 1) and ER beta Delta 5 splice variant mRNAs in sporadic breast cancer",
number = "8",
volume = "133",
pages = "579",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1586"
}

Mandušić, V., Nikolić-Vukosavljević, D., Tanić, N. T., Kanjer, K., Nešković-Konstantinović, Z. B., Celeketić, D. C.,& Dimitrijević, B. B.. (2007). Expression of estrogen receptor beta wt isoform (ER beta 1) and ER beta Delta 5 splice variant mRNAs in sporadic breast cancer. in Journal of Cancer Research and Clinical Oncology, 133(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1586

Mandušić V, Nikolić-Vukosavljević D, Tanić NT, Kanjer K, Nešković-Konstantinović ZB, Celeketić DC, Dimitrijević BB. Expression of estrogen receptor beta wt isoform (ER beta 1) and ER beta Delta 5 splice variant mRNAs in sporadic breast cancer. in Journal of Cancer Research and Clinical Oncology. 2007;133(8):null-579.
https://hdl.handle.net/21.15107/rcub_ibiss_1586 .

Mandušić, Vesna, Nikolić-Vukosavljević, Dragica, Tanić, Nikola T, Kanjer, Ksenija, Nešković-Konstantinović, Zora B., Celeketić, Dusica C, Dimitrijević, Bogomir B., "Expression of estrogen receptor beta wt isoform (ER beta 1) and ER beta Delta 5 splice variant mRNAs in sporadic breast cancer" in Journal of Cancer Research and Clinical Oncology, 133, no. 8 (2007),
https://hdl.handle.net/21.15107/rcub_ibiss_1586 .

TY  - JOUR
AU  - Mandušić, Vesna
AU  - Krtolica-Žikić, Koviljka V.
AU  - Nikolić-Vukosavljević, Dragica
AU  - Popov-Čeleketić, D.
AU  - Plećaš, D.
AU  - Boričić, I.
AU  - Dimitrijević, Bogomir B.
AU  - Tanić, N.
PY  - 2007
PY  - 2007
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/153
T2  - Archives of Biological Sciences
T1  - Transcriptional ratio of estrogen receptor β mRNAs in carcinomas and in normal tissues
IS  - 2
VL  - 59
SP  - 15
EP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_153
ER  - 

@article{
author = "Mandušić, Vesna and Krtolica-Žikić, Koviljka V. and Nikolić-Vukosavljević, Dragica and Popov-Čeleketić, D. and Plećaš, D. and Boričić, I. and Dimitrijević, Bogomir B. and Tanić, N.",
year = "2007, 2007",
journal = "Archives of Biological Sciences",
title = "Transcriptional ratio of estrogen receptor β mRNAs in carcinomas and in normal tissues",
number = "2",
volume = "59",
pages = "15-16",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_153"
}

Mandušić, V., Krtolica-Žikić, K. V., Nikolić-Vukosavljević, D., Popov-Čeleketić, D., Plećaš, D., Boričić, I., Dimitrijević, B. B.,& Tanić, N.. (2007). Transcriptional ratio of estrogen receptor β mRNAs in carcinomas and in normal tissues. in Archives of Biological Sciences, 59(2), 15-16.
https://hdl.handle.net/21.15107/rcub_ibiss_153

Mandušić V, Krtolica-Žikić KV, Nikolić-Vukosavljević D, Popov-Čeleketić D, Plećaš D, Boričić I, Dimitrijević BB, Tanić N. Transcriptional ratio of estrogen receptor β mRNAs in carcinomas and in normal tissues. in Archives of Biological Sciences. 2007;59(2):15-16.
https://hdl.handle.net/21.15107/rcub_ibiss_153 .

Mandušić, Vesna, Krtolica-Žikić, Koviljka V., Nikolić-Vukosavljević, Dragica, Popov-Čeleketić, D., Plećaš, D., Boričić, I., Dimitrijević, Bogomir B., Tanić, N., "Transcriptional ratio of estrogen receptor β mRNAs in carcinomas and in normal tissues" in Archives of Biological Sciences, 59, no. 2 (2007):15-16,
https://hdl.handle.net/21.15107/rcub_ibiss_153 .

TY  - JOUR
AU  - Mandušić, Vesna
AU  - Nikolić-Vukosavljević, Dragica
AU  - Nešković-Konstantinović, Zora B.
AU  - Tanić, Nikola
AU  - Čeleketić, Dušica
AU  - Dimitrijević, Bogomir B.
PY  - 2006
PY  - 2006
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/374
AB  - Background: Estrogen and progesterone receptor (ER/PR) status is an accepted predictive marker in breast cancer. It is well known that breast tumors, which are ER(+) are more likely to respond to endocrine therapy. However, certain percentage of ER(+)/PR(+) tumors do not respond to endocrine therapy. Identification of the second estrogen receptor, named estrogen receptor beta (ERβ), as well as the existence of numerous isoforms/splice variants of both ERα and ERβ, suggests that complex regulation of estrogen action exists. In this study, we analyze does the expression of two ERβ isoforms correlates with ERα/PR status. Methods: Sixty samples of primary operable breast carcinomas were analyzed for ERα and PR protein levels and for mRNA expression of two ERβ isoforms (ERβ1 and ERβΔ5). ERα and PR proteins were measured by classical biochemical techniques, and ERβ mRNAs were measured by real-time RT-PCR. Results: Tumors are divided in three groups according to relative level of mRNA for ERβ1 and ERβΔ5. We found that there is no correlation of ERβ1 mRNA expression with ERα and PR protein levels. We confirmed the existence of inverse correlation of ERβΔ5 with PR and of ERβΔ5 with ERα in the group of postmenopausal patients. In the subsets of tumors defined by ERα/PR status, we found that percentage of tumors, which concomitantly expressed high levels of both transcripts, are parallel with those that do not response to tamoxifen treatment. Conclusion: Inverse correlation of ERα with ERβΔ5 and PR with ERβΔ5isoform suggests that ERβΔ5 may have inhibitory effect on ERα activity in postmenopausal patients. In addition, we point out that determination of expression profiles of ERα and ERβ isoforms in the defined groups of patient are necessary for elucidating its involvement in endocrine resistance.
T2  - Archive of Oncology
T1  - The role of estrogen receptors isoforms in breast cancer
IS  - 3-4
VL  - 14
SP  - 106
EP  - 109
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_374
ER  - 

@article{
author = "Mandušić, Vesna and Nikolić-Vukosavljević, Dragica and Nešković-Konstantinović, Zora B. and Tanić, Nikola and Čeleketić, Dušica and Dimitrijević, Bogomir B.",
year = "2006, 2006",
abstract = "Background: Estrogen and progesterone receptor (ER/PR) status is an accepted predictive marker in breast cancer. It is well known that breast tumors, which are ER(+) are more likely to respond to endocrine therapy. However, certain percentage of ER(+)/PR(+) tumors do not respond to endocrine therapy. Identification of the second estrogen receptor, named estrogen receptor beta (ERβ), as well as the existence of numerous isoforms/splice variants of both ERα and ERβ, suggests that complex regulation of estrogen action exists. In this study, we analyze does the expression of two ERβ isoforms correlates with ERα/PR status. Methods: Sixty samples of primary operable breast carcinomas were analyzed for ERα and PR protein levels and for mRNA expression of two ERβ isoforms (ERβ1 and ERβΔ5). ERα and PR proteins were measured by classical biochemical techniques, and ERβ mRNAs were measured by real-time RT-PCR. Results: Tumors are divided in three groups according to relative level of mRNA for ERβ1 and ERβΔ5. We found that there is no correlation of ERβ1 mRNA expression with ERα and PR protein levels. We confirmed the existence of inverse correlation of ERβΔ5 with PR and of ERβΔ5 with ERα in the group of postmenopausal patients. In the subsets of tumors defined by ERα/PR status, we found that percentage of tumors, which concomitantly expressed high levels of both transcripts, are parallel with those that do not response to tamoxifen treatment. Conclusion: Inverse correlation of ERα with ERβΔ5 and PR with ERβΔ5isoform suggests that ERβΔ5 may have inhibitory effect on ERα activity in postmenopausal patients. In addition, we point out that determination of expression profiles of ERα and ERβ isoforms in the defined groups of patient are necessary for elucidating its involvement in endocrine resistance.",
journal = "Archive of Oncology",
title = "The role of estrogen receptors isoforms in breast cancer",
number = "3-4",
volume = "14",
pages = "106-109",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_374"
}

Mandušić, V., Nikolić-Vukosavljević, D., Nešković-Konstantinović, Z. B., Tanić, N., Čeleketić, D.,& Dimitrijević, B. B.. (2006). The role of estrogen receptors isoforms in breast cancer. in Archive of Oncology, 14(3-4), 106-109.
https://hdl.handle.net/21.15107/rcub_ibiss_374

Mandušić V, Nikolić-Vukosavljević D, Nešković-Konstantinović ZB, Tanić N, Čeleketić D, Dimitrijević BB. The role of estrogen receptors isoforms in breast cancer. in Archive of Oncology. 2006;14(3-4):106-109.
https://hdl.handle.net/21.15107/rcub_ibiss_374 .

Mandušić, Vesna, Nikolić-Vukosavljević, Dragica, Nešković-Konstantinović, Zora B., Tanić, Nikola, Čeleketić, Dušica, Dimitrijević, Bogomir B., "The role of estrogen receptors isoforms in breast cancer" in Archive of Oncology, 14, no. 3-4 (2006):106-109,
https://hdl.handle.net/21.15107/rcub_ibiss_374 .

TY  - JOUR
AU  - Tanić, Nikola T
AU  - Vujošević, Mladen
AU  - Dedović-Tanić, Nasta
AU  - Dimitrijević, Bogomir B.
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1706
AB  - Most B chromosomes are heavily heterochromatic, promoting the general idea that they are genetically inert. The B chromosomes of Apodemus flavicollis are euchromatic and show a high degree of homology with the chromosomes. The euchromatic nature of chromosomes in A. flavicollis suggests that they may carry active genes and have transcriptional activity. We applied the differential display reverse transcription-polymerase chain reaction (DD RT-PCR) in order to analyze and compare gene expression in animals possessing chromosomes and animals without B chromosomes. After a second and third round of amplification, three cDNA fragments were differentially expressed in +B mice compared with 0B animals. These cDNAs were Chaperonin containing TCP-1, subunit 6b (zeta) (CCT6B), Fragile histidine triad gene (FHIT) and hypothetical gene XP transcript. The differential expression pattern was confirmed by Real Time RT-PCR. We suggest that altered expression of these important genes is due to the presence of B chromosomes. In elevating the expression of these genes, B chromosomes of A. flavicollis affect some of the crucial processes in the cell. The significance of these effects and the nature of B chromosomes of A. flavicollis are discussed in the context of the data presented.
T2  - Chromosoma
T1  - Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes
IS  - 8
VL  - 113
EP  - 427
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1706
ER  - 

@article{
author = "Tanić, Nikola T and Vujošević, Mladen and Dedović-Tanić, Nasta and Dimitrijević, Bogomir B.",
year = "2005",
abstract = "Most B chromosomes are heavily heterochromatic, promoting the general idea that they are genetically inert. The B chromosomes of Apodemus flavicollis are euchromatic and show a high degree of homology with the chromosomes. The euchromatic nature of chromosomes in A. flavicollis suggests that they may carry active genes and have transcriptional activity. We applied the differential display reverse transcription-polymerase chain reaction (DD RT-PCR) in order to analyze and compare gene expression in animals possessing chromosomes and animals without B chromosomes. After a second and third round of amplification, three cDNA fragments were differentially expressed in +B mice compared with 0B animals. These cDNAs were Chaperonin containing TCP-1, subunit 6b (zeta) (CCT6B), Fragile histidine triad gene (FHIT) and hypothetical gene XP transcript. The differential expression pattern was confirmed by Real Time RT-PCR. We suggest that altered expression of these important genes is due to the presence of B chromosomes. In elevating the expression of these genes, B chromosomes of A. flavicollis affect some of the crucial processes in the cell. The significance of these effects and the nature of B chromosomes of A. flavicollis are discussed in the context of the data presented.",
journal = "Chromosoma",
title = "Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes",
number = "8",
volume = "113",
pages = "427",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1706"
}

Tanić, N. T., Vujošević, M., Dedović-Tanić, N.,& Dimitrijević, B. B.. (2005). Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes. in Chromosoma, 113(8).
https://hdl.handle.net/21.15107/rcub_ibiss_1706

Tanić NT, Vujošević M, Dedović-Tanić N, Dimitrijević BB. Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes. in Chromosoma. 2005;113(8):null-427.
https://hdl.handle.net/21.15107/rcub_ibiss_1706 .

Tanić, Nikola T, Vujošević, Mladen, Dedović-Tanić, Nasta, Dimitrijević, Bogomir B., "Differential gene expression in yellow-necked mice Apodemus flavicollis (Rodentia, Mammalia) with and without B chromosomes" in Chromosoma, 113, no. 8 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1706 .

TY  - JOUR
AU  - Tanić, Nikola T
AU  - Dedović, Nasta
AU  - Vujošević, Mladen
AU  - Dimitrijević, Bogomir B.
PY  - 2000
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1836
AB  - Using AP-PCR-based DNA profiling we examined some structural features of B chromosomes from yellow-necked mice Apodemus flavicollis. Mice harboring one,two, or three or lacking B chromosomes were examined. Chromosomal structure was scanned for variant bands by using a series of arbitrary primers and From these, informative bands were selected. The selection:criteria used were the ability to differentiate between individuals of the species, to detect markers common For both A and B chromosomes, and, importantly, to differentiate between A- and B-chromosome sets. In addition to primers, profiling conditions were found to be critical for meeting the selection criteria. Primers and analysis conditions that demonstrated structural characteristics unique to the B-chromosome set are described. These characteristics included variant bands as qualitative parameters and altered electrophoretic band-intensities as quantitative distinctions estimated by integration of densitometric profiles of electrophoretograms. B chromosome-specific molecular markers are easy to detect by AP-PCR-based DNA profiling in the presence of a full set of A chromosomes. Models for the origin of yellow-necked mouse B chromosomes are discussed in the context of presented data.
T2  - Genome Research
T1  - DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia)
IS  - 1
VL  - 10
EP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1836
ER  - 

@article{
author = "Tanić, Nikola T and Dedović, Nasta and Vujošević, Mladen and Dimitrijević, Bogomir B.",
year = "2000",
abstract = "Using AP-PCR-based DNA profiling we examined some structural features of B chromosomes from yellow-necked mice Apodemus flavicollis. Mice harboring one,two, or three or lacking B chromosomes were examined. Chromosomal structure was scanned for variant bands by using a series of arbitrary primers and From these, informative bands were selected. The selection:criteria used were the ability to differentiate between individuals of the species, to detect markers common For both A and B chromosomes, and, importantly, to differentiate between A- and B-chromosome sets. In addition to primers, profiling conditions were found to be critical for meeting the selection criteria. Primers and analysis conditions that demonstrated structural characteristics unique to the B-chromosome set are described. These characteristics included variant bands as qualitative parameters and altered electrophoretic band-intensities as quantitative distinctions estimated by integration of densitometric profiles of electrophoretograms. B chromosome-specific molecular markers are easy to detect by AP-PCR-based DNA profiling in the presence of a full set of A chromosomes. Models for the origin of yellow-necked mouse B chromosomes are discussed in the context of presented data.",
journal = "Genome Research",
title = "DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia)",
number = "1",
volume = "10",
pages = "61",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1836"
}

Tanić, N. T., Dedović, N., Vujošević, M.,& Dimitrijević, B. B.. (2000). DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia). in Genome Research, 10(1).
https://hdl.handle.net/21.15107/rcub_ibiss_1836

Tanić NT, Dedović N, Vujošević M, Dimitrijević BB. DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia). in Genome Research. 2000;10(1):null-61.
https://hdl.handle.net/21.15107/rcub_ibiss_1836 .

Tanić, Nikola T, Dedović, Nasta, Vujošević, Mladen, Dimitrijević, Bogomir B., "DNA profiling of B chromosomes from the yellow-necked mouse Apodemus flavicollis (Rodentia, Mammalia)" in Genome Research, 10, no. 1 (2000),
https://hdl.handle.net/21.15107/rcub_ibiss_1836 .