TY  - CONF
AU  - Čukuranović-Kokoris, Jovana
AU  - Dovenska, Monika
AU  - Ajdžanović, Vladimir
AU  - Marković Filipović, Jelena
AU  - Ugrenović, Slađana
AU  - Kundalić, Braca
AU  - Milošević, Verica
AU  - Popovska-Perčinić, Florina
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6278
AB  - Uvod. Faktori sredine, kao što je visoka ili niska temperatura ambijenta, predstavljaju snažne stresore za organizam. Hipofizne ćelije koje luče hormone, posebno somatotropne (GH), intenzivno reaguju na ove faktore. Cilj ove studije bio je da ispita efekat dugotrajnog (60-dnevnog) izlaganja pacova umereno visokoj temperaturi ambijenta (35±1°C) na imunohistomorfometrijske parametre GH ćelija hipofize.
Metode. Eksperiment je izveden na odraslim Wistar pacovima, podeljenim u dve jednake grupe: sedam pacova je kontinuirano bilo izloženo temperaturi 35± l°C tokom 60 dana (eksperimentalna grupa), dok je sedam pacova tokom istog perioda čuvano na 20±2°C (kontrolna grupa). Sve su životinje čuvane u režimu od 12 sati dnevne svetlosti i 12 sati mraka, dok su hranu i vodu mogle da uzimaju ad libitum. GH ćelije vizuelizovane su pomoću imunohistohemijske procedure peroksidaze-antiperoksidaze (PAP). Morfometrijska analiza izvedena je korišćenjem višenamenskog M42 test-sistema.
Rezultati. Nakon 60 dana izlaganja povišenoj ambijentalnoj temperaturi, telesna masa životinja bila je značajno smanjena za 16,4%, u poređenju sa kontrolama. GH ćelije u kontrolnoj i eksperimentalnoj grupi bile su ovalnog oblika i locirane u blizini kapilara. Tamne granule, distribuirane po citoplazmi GH ćelija, bile su sebi svojstvenih univer­zalnih karakteristika u obe grupe. Kod eksperimentalnih pacova, celularni i nukleami volumeni GH ćelija, kao i volumenska gustina GH ćelija, značajno su redukovani za 19,1%, 10,6% i 15,8%, redom, u poređenju sa intaktnim kontrolama.
Zaključak. Izlaganje odraslih pacova muškog pola umereno visokoj ambijentalnoj temperaturi u trajanju od 60 dana dovelo je do smanjenja histomorfometrijskih parametara GH ćelija.
PB  - Niš: University of Niš, Faculty of Medicine
C3  - Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
T1  - Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize
SP  - 160
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_6278
ER  - 

@conference{
author = "Čukuranović-Kokoris, Jovana and Dovenska, Monika and Ajdžanović, Vladimir and Marković Filipović, Jelena and Ugrenović, Slađana and Kundalić, Braca and Milošević, Verica and Popovska-Perčinić, Florina",
year = "2023",
abstract = "Uvod. Faktori sredine, kao što je visoka ili niska temperatura ambijenta, predstavljaju snažne stresore za organizam. Hipofizne ćelije koje luče hormone, posebno somatotropne (GH), intenzivno reaguju na ove faktore. Cilj ove studije bio je da ispita efekat dugotrajnog (60-dnevnog) izlaganja pacova umereno visokoj temperaturi ambijenta (35±1°C) na imunohistomorfometrijske parametre GH ćelija hipofize.
Metode. Eksperiment je izveden na odraslim Wistar pacovima, podeljenim u dve jednake grupe: sedam pacova je kontinuirano bilo izloženo temperaturi 35± l°C tokom 60 dana (eksperimentalna grupa), dok je sedam pacova tokom istog perioda čuvano na 20±2°C (kontrolna grupa). Sve su životinje čuvane u režimu od 12 sati dnevne svetlosti i 12 sati mraka, dok su hranu i vodu mogle da uzimaju ad libitum. GH ćelije vizuelizovane su pomoću imunohistohemijske procedure peroksidaze-antiperoksidaze (PAP). Morfometrijska analiza izvedena je korišćenjem višenamenskog M42 test-sistema.
Rezultati. Nakon 60 dana izlaganja povišenoj ambijentalnoj temperaturi, telesna masa životinja bila je značajno smanjena za 16,4%, u poređenju sa kontrolama. GH ćelije u kontrolnoj i eksperimentalnoj grupi bile su ovalnog oblika i locirane u blizini kapilara. Tamne granule, distribuirane po citoplazmi GH ćelija, bile su sebi svojstvenih univer­zalnih karakteristika u obe grupe. Kod eksperimentalnih pacova, celularni i nukleami volumeni GH ćelija, kao i volumenska gustina GH ćelija, značajno su redukovani za 19,1%, 10,6% i 15,8%, redom, u poređenju sa intaktnim kontrolama.
Zaključak. Izlaganje odraslih pacova muškog pola umereno visokoj ambijentalnoj temperaturi u trajanju od 60 dana dovelo je do smanjenja histomorfometrijskih parametara GH ćelija.",
publisher = "Niš: University of Niš, Faculty of Medicine",
journal = "Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia",
title = "Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize",
pages = "160",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_6278"
}

Čukuranović-Kokoris, J., Dovenska, M., Ajdžanović, V., Marković Filipović, J., Ugrenović, S., Kundalić, B., Milošević, V.,& Popovska-Perčinić, F.. (2023). Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia
Niš: University of Niš, Faculty of Medicine., 160.
https://hdl.handle.net/21.15107/rcub_ibiss_6278

Čukuranović-Kokoris J, Dovenska M, Ajdžanović V, Marković Filipović J, Ugrenović S, Kundalić B, Milošević V, Popovska-Perčinić F. Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize. in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia. 2023;:160.
https://hdl.handle.net/21.15107/rcub_ibiss_6278 .

Čukuranović-Kokoris, Jovana, Dovenska, Monika, Ajdžanović, Vladimir, Marković Filipović, Jelena, Ugrenović, Slađana, Kundalić, Braca, Milošević, Verica, Popovska-Perčinić, Florina, "Efekat dugotrajnog izlaganja umereno visokim temperaturama ambijenta na imunohistomorfometrijske parametre GH ćelija hipofize" in Abstract Book: 8th Congress of Serbian Anatomical Society of Serbia with international participation; 2023 Sep 21-23; Niš, Serbia (2023):160,
https://hdl.handle.net/21.15107/rcub_ibiss_6278 .

TY  - JOUR
AU  - Čukuranović Kokoris, Jovana
AU  - Ajdžanović, Vladimir
AU  - Pendovski, Lazo
AU  - Ristić, Nataša
AU  - Milošević, Verica
AU  - Dovenska, Monika
AU  - Popovska-Perčinić, Florina
PY  - 2022
UR  - https://www.sciendo.com/article/10.2478/acve-2022-0001
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/4965
AB  - Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.
T2  - Acta Veterinaria
T1  - The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study
IS  - 1
VL  - 72
DO  - 10.2478/acve-2022-0001
SP  - 1
EP  - 15
ER  - 

@article{
author = "Čukuranović Kokoris, Jovana and Ajdžanović, Vladimir and Pendovski, Lazo and Ristić, Nataša and Milošević, Verica and Dovenska, Monika and Popovska-Perčinić, Florina",
year = "2022",
abstract = "Global warming causes an increased ambient temperature and prolonged heatwaves during the summer, which represent stressogenic factors affecting the hypothalamo-pituitary-adrenocortical (HPA) axis in mammals. The aim of this study was to investigate the effects of long-term (7-60 days) exposure to moderately elevated ambient temperature (35 ± 1°C) on the histological aspect and secretory ability of pituitary adrenocorticotropic (ACTH) cells, as well as on the corticosterone output, in adult rats. Stereological parameters of ACTH cells were estimated upon immunohistochemistry. The blood concentrations of ACTH and corticosterone were determined by immunoassays. The volume of ACTH cells in rats exposed to moderately high temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 18.1%, 14.5%, 13.5%, 8.6% and 14.2% respectively, compared to the same parameter in the controls. The volume density of ACTH cells in the groups exposed to elevated temperature for 7, 14, 21, 30 and 60 days decreased (p<0.05) by 40.0%, 33.3%, 26.7%, 13.3% and 26.7% respectively, in comparison with control rats. The plasma concentration of ACTH varied differently (p<0.05) with the duration of exposure to the elevated temperature. The serum concentration of corticosterone was decreased (p<0.05) by 54.9%, 24.4%, 29.9%, 21.1% and 24.4% in groups subjected to moderately high temperature for 7, 14, 21, 30 and 60 days respectively, all compared to the control value. Despite some signs of functional recovery of ACTH cells during the treatment, the impression is that the long-term character of this stressor overcomes the capacity of the HPA axis for resistance.",
journal = "Acta Veterinaria",
title = "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study",
number = "1",
volume = "72",
doi = "10.2478/acve-2022-0001",
pages = "1-15"
}

Čukuranović Kokoris, J., Ajdžanović, V., Pendovski, L., Ristić, N., Milošević, V., Dovenska, M.,& Popovska-Perčinić, F.. (2022). The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria, 72(1), 1-15.
https://doi.org/10.2478/acve-2022-0001

Čukuranović Kokoris J, Ajdžanović V, Pendovski L, Ristić N, Milošević V, Dovenska M, Popovska-Perčinić F. The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study. in Acta Veterinaria. 2022;72(1):1-15.
doi:10.2478/acve-2022-0001 .

Čukuranović Kokoris, Jovana, Ajdžanović, Vladimir, Pendovski, Lazo, Ristić, Nataša, Milošević, Verica, Dovenska, Monika, Popovska-Perčinić, Florina, "The Effects of Long-Term Exposure to Moderate Heat on Rat Pituitary ACTH Cells: Histological and Hormonal Study" in Acta Veterinaria, 72, no. 1 (2022):1-15,
https://doi.org/10.2478/acve-2022-0001 . .