TY  - JOUR
AU  - Stevanović, Katarina S
AU  - Čepkenović, Bogdana
AU  - Križak, Strahinja
AU  - Pajić, Tanja
AU  - Todorović, Nataša
AU  - Živić, Miroslav Ž
PY  - 2023
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6088
AB  - Ion channels are vital components of filamentous fungi signaling in communication with their environment. We exploited the ability of the apical region of growing sporangiophores of Phycomyces blakesleeanus to form membrane-enveloped cytoplasmic droplets (CDs), to examine ion currents in the filamentous fungi native plasma membrane. In hypoosmotic conditions, the dominant current in the CDs is ORIC, an osmotically activated, anionic, outwardly rectified, fast inactivating instantaneous current that we have previously characterized. Here, we examined the effect of ATP on ORIC. We show that CDs contain active mitochondria, and that respiration inhibition by azide accelerates ORIC inactivation. ATP, added intracellularly, reduced ORIC run-down and shifted the voltage dependence of inactivation toward depolarized potentials, in a manner that did not require hydrolysis. Notably, ATP led to slowing down of ORIC inactivation, as evidenced by an increased time constant of inactivation, τin, and slower decline of τin during prolonged recordings. Flavonoids (genistein and quercetin) had the effect on ORIC opposite to ATP, acting as current inhibitors, possibly by disrupting the stabilizing effect of ATP on ORIC. The integration of osmotic sensing with ATP dependence of the anionic current, typical of vertebrate cells, is described here for the first time in filamentous fungi.
PB  - Springer Nature
T2  - Scientific Reports
T1  - ATP modulation of osmotically activated anionic current in the membrane of Phycomyces blakesleeanus sporangiophore
IS  - 1
VL  - 13
DO  - 10.1038/s41598-023-39021-9
SP  - 11897
ER  - 

@article{
author = "Stevanović, Katarina S and Čepkenović, Bogdana and Križak, Strahinja and Pajić, Tanja and Todorović, Nataša and Živić, Miroslav Ž",
year = "2023",
abstract = "Ion channels are vital components of filamentous fungi signaling in communication with their environment. We exploited the ability of the apical region of growing sporangiophores of Phycomyces blakesleeanus to form membrane-enveloped cytoplasmic droplets (CDs), to examine ion currents in the filamentous fungi native plasma membrane. In hypoosmotic conditions, the dominant current in the CDs is ORIC, an osmotically activated, anionic, outwardly rectified, fast inactivating instantaneous current that we have previously characterized. Here, we examined the effect of ATP on ORIC. We show that CDs contain active mitochondria, and that respiration inhibition by azide accelerates ORIC inactivation. ATP, added intracellularly, reduced ORIC run-down and shifted the voltage dependence of inactivation toward depolarized potentials, in a manner that did not require hydrolysis. Notably, ATP led to slowing down of ORIC inactivation, as evidenced by an increased time constant of inactivation, τin, and slower decline of τin during prolonged recordings. Flavonoids (genistein and quercetin) had the effect on ORIC opposite to ATP, acting as current inhibitors, possibly by disrupting the stabilizing effect of ATP on ORIC. The integration of osmotic sensing with ATP dependence of the anionic current, typical of vertebrate cells, is described here for the first time in filamentous fungi.",
publisher = "Springer Nature",
journal = "Scientific Reports",
title = "ATP modulation of osmotically activated anionic current in the membrane of Phycomyces blakesleeanus sporangiophore",
number = "1",
volume = "13",
doi = "10.1038/s41598-023-39021-9",
pages = "11897"
}

Stevanović, K. S., Čepkenović, B., Križak, S., Pajić, T., Todorović, N.,& Živić, M. Ž.. (2023). ATP modulation of osmotically activated anionic current in the membrane of Phycomyces blakesleeanus sporangiophore. in Scientific Reports
Springer Nature., 13(1), 11897.
https://doi.org/10.1038/s41598-023-39021-9

Stevanović KS, Čepkenović B, Križak S, Pajić T, Todorović N, Živić MŽ. ATP modulation of osmotically activated anionic current in the membrane of Phycomyces blakesleeanus sporangiophore. in Scientific Reports. 2023;13(1):11897.
doi:10.1038/s41598-023-39021-9 .

Stevanović, Katarina S, Čepkenović, Bogdana, Križak, Strahinja, Pajić, Tanja, Todorović, Nataša, Živić, Miroslav Ž, "ATP modulation of osmotically activated anionic current in the membrane of Phycomyces blakesleeanus sporangiophore" in Scientific Reports, 13, no. 1 (2023):11897,
https://doi.org/10.1038/s41598-023-39021-9 . .

TY  - JOUR
AU  - Avdović, Edina H.
AU  - Petrović, Isidora P.
AU  - Stevanović, Milena J.
AU  - Saso, Luciano
AU  - Dimitrić Marković, Jasmina M.
AU  - Filipović, Nenad D.
AU  - Živić, Miroslav Ž.
AU  - Cvetić Antić, Tijana N.
AU  - Žižić, Milan V.
AU  - Todorović, Nataša
AU  - Vukić, Milena
AU  - Trifunović, Srećko R.
AU  - Marković, Zoran S.
PY  - 2021
UR  - https://www.hindawi.com/journals/omcl/2021/8849568/
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/4244
AB  - Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, 1H NMR, 13C NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards •OH and -•OOH radicals and anti-ABTS (2,2 ′ -Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells’ viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.
PB  - Hindawi Limited
T2  - Oxidative Medicine and Cellular Longevity
T1  - Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes
VL  - 2021
DO  - 10.1155/2021/8849568
SP  - 8849568
ER  - 

@article{
author = "Avdović, Edina H. and Petrović, Isidora P. and Stevanović, Milena J. and Saso, Luciano and Dimitrić Marković, Jasmina M. and Filipović, Nenad D. and Živić, Miroslav Ž. and Cvetić Antić, Tijana N. and Žižić, Milan V. and Todorović, Nataša and Vukić, Milena and Trifunović, Srećko R. and Marković, Zoran S.",
year = "2021",
abstract = "Two newly synthesized 4-hydroxycoumarin bidentate ligands (L1 and L2) and their palladium(II) complexes (C1 and C2) were screened for their biological activities, in vitro and in vivo. Structures of new compounds were established based on elemental analysis, 1H NMR, 13C NMR, and IR spectroscopic techniques. The obtained compounds were tested for their antioxidative and cytotoxic activities and results pointed to selective antiradical activity of palladium(II) complexes towards •OH and -•OOH radicals and anti-ABTS (2,2 ′ -Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) cation radical) activity comparable to that of ascorbate. Results indicated the effect of C1 and C2 on the enzymatic activity of the antioxidative defense system. In vitro cytotoxicity assay performed on different carcinoma cell lines (HCT166, A375, and MIA PaCa-2), and one healthy fibroblast cell line (MRC-5) showed a cytotoxic effect of both C1 and C2, expressed as a decrease in carcinoma cells’ viability, mostly by induction of apoptosis. In vivo toxicity tests performed on zebrafish embryos indicated different effects of C1 and C2, ranging from adverse developmental effect to no toxicity, depending on tested concentration. According to docking studies, both complexes (C1 and C2) showed better inhibitory activity in comparison to other palladium(II) complexes.",
publisher = "Hindawi Limited",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes",
volume = "2021",
doi = "10.1155/2021/8849568",
pages = "8849568"
}

Avdović, E. H., Petrović, I. P., Stevanović, M. J., Saso, L., Dimitrić Marković, J. M., Filipović, N. D., Živić, M. Ž., Cvetić Antić, T. N., Žižić, M. V., Todorović, N., Vukić, M., Trifunović, S. R.,& Marković, Z. S.. (2021). Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity
Hindawi Limited., 2021, 8849568.
https://doi.org/10.1155/2021/8849568

Avdović EH, Petrović IP, Stevanović MJ, Saso L, Dimitrić Marković JM, Filipović ND, Živić MŽ, Cvetić Antić TN, Žižić MV, Todorović N, Vukić M, Trifunović SR, Marković ZS. Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes. in Oxidative Medicine and Cellular Longevity. 2021;2021:8849568.
doi:10.1155/2021/8849568 .

Avdović, Edina H., Petrović, Isidora P., Stevanović, Milena J., Saso, Luciano, Dimitrić Marković, Jasmina M., Filipović, Nenad D., Živić, Miroslav Ž., Cvetić Antić, Tijana N., Žižić, Milan V., Todorović, Nataša, Vukić, Milena, Trifunović, Srećko R., Marković, Zoran S., "Synthesis and Biological Screening of New 4-Hydroxycoumarin Derivatives and Their Palladium(II) Complexes" in Oxidative Medicine and Cellular Longevity, 2021 (2021):8849568,
https://doi.org/10.1155/2021/8849568 . .

TY  - JOUR
AU  - Božanić, Milenka L.J.
AU  - Todorović, Dajana
AU  - Živić, Miroslav Ž.
AU  - Perić Mataruga, Vesna
AU  - Marković, Zoran Z.
AU  - Živić, Ivana M.
PY  - 2018
UR  - https://www.kmae-journal.org/10.1051/kmae/2018036
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/3211
AB  - The aim of this study was to establish the influence of a trout farm on the activity of enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), and total glutathione concentration (GSH), in larvae of Ephemera danica, and on environmental parameters in the receiving watercourse. The investigations were carried out seasonally (April, July, and October of 2016 and January of 2017) at four localities (SK1–control, and SK2, SK3, and SK4–30 m, 330 m and 630 m below the fish farm, respectively) along the Skrapež River. Discharged water had the greatest effect on the concentrations of dissolved oxygen (DO), ammonium ions, nitrates (NO3−), and total phosphorus. In the case of SOD and GSH, seasonal changes were greater than longitudinal, with maximal values attained in spring and summer and minimal in autumn and winter. SOD showed the strongest correlation with DO, and GSH with total nitrogen. Activity of GPx demonstrated greater longitudinal variability with maximum at SK2 in all seasons and the strongest correlation with NO3−. The obtained results indicate that GPx activity was the most sensitive to the trout farm effects, while SOD and GSH were more influenced by natural seasonal changes of environmental parameters.
PB  - EDP Sciences
T2  - Knowledge and Management of Aquatic Ecosystems
T1  - Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera)
IS  - 419
DO  - 10.1051/kmae/2018036
SP  - 47
ER  - 

@article{
author = "Božanić, Milenka L.J. and Todorović, Dajana and Živić, Miroslav Ž. and Perić Mataruga, Vesna and Marković, Zoran Z. and Živić, Ivana M.",
year = "2018",
abstract = "The aim of this study was to establish the influence of a trout farm on the activity of enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx), and total glutathione concentration (GSH), in larvae of Ephemera danica, and on environmental parameters in the receiving watercourse. The investigations were carried out seasonally (April, July, and October of 2016 and January of 2017) at four localities (SK1–control, and SK2, SK3, and SK4–30 m, 330 m and 630 m below the fish farm, respectively) along the Skrapež River. Discharged water had the greatest effect on the concentrations of dissolved oxygen (DO), ammonium ions, nitrates (NO3−), and total phosphorus. In the case of SOD and GSH, seasonal changes were greater than longitudinal, with maximal values attained in spring and summer and minimal in autumn and winter. SOD showed the strongest correlation with DO, and GSH with total nitrogen. Activity of GPx demonstrated greater longitudinal variability with maximum at SK2 in all seasons and the strongest correlation with NO3−. The obtained results indicate that GPx activity was the most sensitive to the trout farm effects, while SOD and GSH were more influenced by natural seasonal changes of environmental parameters.",
publisher = "EDP Sciences",
journal = "Knowledge and Management of Aquatic Ecosystems",
title = "Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera)",
number = "419",
doi = "10.1051/kmae/2018036",
pages = "47"
}

Božanić, M. L.J., Todorović, D., Živić, M. Ž., Perić Mataruga, V., Marković, Z. Z.,& Živić, I. M.. (2018). Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera). in Knowledge and Management of Aquatic Ecosystems
EDP Sciences.(419), 47.
https://doi.org/10.1051/kmae/2018036

Božanić ML, Todorović D, Živić MŽ, Perić Mataruga V, Marković ZZ, Živić IM. Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera). in Knowledge and Management of Aquatic Ecosystems. 2018;(419):47.
doi:10.1051/kmae/2018036 .

Božanić, Milenka L.J., Todorović, Dajana, Živić, Miroslav Ž., Perić Mataruga, Vesna, Marković, Zoran Z., Živić, Ivana M., "Influence of a trout farm on antioxidant defense in larvae of Ephemera danica (Insecta: Ephemeroptera)" in Knowledge and Management of Aquatic Ecosystems, no. 419 (2018):47,
https://doi.org/10.1051/kmae/2018036 . .