TY  - JOUR
AU  - Šošić-Jurjević, Branka
AU  - Borković-Mitić, Slavica
AU  - Pavlović, Slađan
AU  - Vlahović, Dragana
AU  - Miler, Marko
AU  - Cesar, Thais
AU  - Ajdžanović, Vladimir
AU  - Milenković, Dragan
AU  - Stellaard, Frans
AU  - Trifunović, Svetlana
AU  - Filipović, Branko
AU  - Dieter, Lutjohann
PY  - 2024
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6702
AB  - This study aimed to assess the antioxidant capacity of lemon flavonoid extract Eriomin®
(LE) and its impact on cholesterol metabolism in the context of healthy aging. We orally treated 24-
month-old male Wistar rats with an LE (40 mg/kg) suspended in 0.3 mL of sunflower oil. At the
same time, control groups received an equal volume of sunflower oil (CON) or remained untreated
(ICON) daily for 4 weeks. We examined LE’s effects on superoxide dismutase and catalase- and
glutathione-related enzyme activities, the concentration of lipid peroxides and protein carbonyls,
total oxidant status (TOS) and antioxidant status (TAS), and oxidative stress index (OSI) in the liver,
jejunum, and ileum. We also measured total cholesterol, its biosynthetic precursors (lanosterol,
lathosterol, desmosterol), its degradation products (bile acid precursors) in the serum, liver, jejunum, and ileum, and serum phytosterols (intestinal absorption markers). LE reduced TOS, TAS, and
OSI (p < 0.05) compared with control values, indicating its consistent antioxidant action in all examined organs. LE lowered hepatic desmosterol (p < 0.05) while also reducing 7α- and 24-hydroxycholesterol levels in the liver and ileum (p < 0.01). Serum cholesterol, hepatic gene expression, and the
immunostaining intensity of CYP7A1 were unchanged. In conclusion, LE exerted non-enzymatic
antioxidant effects and reduced cholesterol degradation, reducing its biosynthesis products, thereby
maintaining serum cholesterol levels.
PB  - Basel: MDPI
T2  - International Journal of Molecular Sciences
T1  - Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats
IS  - 10
VL  - 25
DO  - 10.3390/ijms25105221
SP  - 5221
ER  - 

@article{
author = "Šošić-Jurjević, Branka and Borković-Mitić, Slavica and Pavlović, Slađan and Vlahović, Dragana and Miler, Marko and Cesar, Thais and Ajdžanović, Vladimir and Milenković, Dragan and Stellaard, Frans and Trifunović, Svetlana and Filipović, Branko and Dieter, Lutjohann",
year = "2024",
abstract = "This study aimed to assess the antioxidant capacity of lemon flavonoid extract Eriomin®
(LE) and its impact on cholesterol metabolism in the context of healthy aging. We orally treated 24-
month-old male Wistar rats with an LE (40 mg/kg) suspended in 0.3 mL of sunflower oil. At the
same time, control groups received an equal volume of sunflower oil (CON) or remained untreated
(ICON) daily for 4 weeks. We examined LE’s effects on superoxide dismutase and catalase- and
glutathione-related enzyme activities, the concentration of lipid peroxides and protein carbonyls,
total oxidant status (TOS) and antioxidant status (TAS), and oxidative stress index (OSI) in the liver,
jejunum, and ileum. We also measured total cholesterol, its biosynthetic precursors (lanosterol,
lathosterol, desmosterol), its degradation products (bile acid precursors) in the serum, liver, jejunum, and ileum, and serum phytosterols (intestinal absorption markers). LE reduced TOS, TAS, and
OSI (p < 0.05) compared with control values, indicating its consistent antioxidant action in all examined organs. LE lowered hepatic desmosterol (p < 0.05) while also reducing 7α- and 24-hydroxycholesterol levels in the liver and ileum (p < 0.01). Serum cholesterol, hepatic gene expression, and the
immunostaining intensity of CYP7A1 were unchanged. In conclusion, LE exerted non-enzymatic
antioxidant effects and reduced cholesterol degradation, reducing its biosynthesis products, thereby
maintaining serum cholesterol levels.",
publisher = "Basel: MDPI",
journal = "International Journal of Molecular Sciences",
title = "Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats",
number = "10",
volume = "25",
doi = "10.3390/ijms25105221",
pages = "5221"
}

Šošić-Jurjević, B., Borković-Mitić, S., Pavlović, S., Vlahović, D., Miler, M., Cesar, T., Ajdžanović, V., Milenković, D., Stellaard, F., Trifunović, S., Filipović, B.,& Dieter, L.. (2024). Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats. in International Journal of Molecular Sciences
Basel: MDPI., 25(10), 5221.
https://doi.org/10.3390/ijms25105221

Šošić-Jurjević B, Borković-Mitić S, Pavlović S, Vlahović D, Miler M, Cesar T, Ajdžanović V, Milenković D, Stellaard F, Trifunović S, Filipović B, Dieter L. Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats. in International Journal of Molecular Sciences. 2024;25(10):5221.
doi:10.3390/ijms25105221 .

Šošić-Jurjević, Branka, Borković-Mitić, Slavica, Pavlović, Slađan, Vlahović, Dragana, Miler, Marko, Cesar, Thais, Ajdžanović, Vladimir, Milenković, Dragan, Stellaard, Frans, Trifunović, Svetlana, Filipović, Branko, Dieter, Lutjohann, "Lemon Flavonoid Extract Eriomin Improves Pro/Antioxidant Status and Interferes with Cholesterol Metabolism without Affecting Serum Cholesterol Levels in Aged Rats" in International Journal of Molecular Sciences, 25, no. 10 (2024):5221,
https://doi.org/10.3390/ijms25105221 . .

TY  - JOUR
AU  - Miler, Marko
AU  - Živanović, Jasmina
AU  - Ajdžanović, Vladimir
AU  - Milenkovic, Dragan
AU  - Cesar, Thais
AU  - Filipović R., Miloš
AU  - Milošević, Verica
PY  - 2024
UR  - https://iubmb.onlinelibrary.wiley.com/journal/18728081
UR  - http://radar.ibiss.bg.ac.rs/handle/123456789/6462
AB  - Citrus flavanones are recognized as promising bioactives within the concept of healthy aging. Thus, the present study investigated the effects of a nutritionally relevant dose of lemon extract (LE) on liver redox regulation and persulfidation levels in 24-month-old Wistar rats. LE (40 mg/kg b.m.) was administered orally once daily for four weeks. Control groups received either vehicle (sunflower oil) or remained intact. The applied methodology considered qPCR, Western blot, protein persulfidation levels evaluation, histochemistry in line with immunofluorescence, liver biochemical assays (glutathione, total -SH groups and malonaldehyde; MDA), liver enzymes in serum and in silico analysis to explore the potential interaction/binding between the proteins studied in the paper. Our results showed that LE increased glutathione peroxidase (GPx), reductase (GR), glutamate-cysteine ligase catalytic and modifier subunit, respectively, as well as Nrf2 gene expressions, but decreased the expression of superoxide dismutase 2 (SOD2). Upon LE application, protein expression showed upregulation of NRF2, SOD2, GPx, GR, and thioredoxin 1 (Trx1). LE significantly decreased the protein persulfidation levels and concentration of MDA, a marker of oxidative damage in the cell. Histological analysis showed a normal liver histoarchitecture without pathological changes, aligning with the normal serum level of hepatic enzymes. Obtained results showed that LE, by modulating hepatic redox regulators Nrf2 and Trx1, diminishes oxidative stress and alters the persulfidation levels, suggesting a considerable beneficial antioxidant potential of lemon flavanones in the old-aged liver.
PB  - John Wiley and Sons
T2  - Biofactors
T1  - Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats
DO  - 10.1002/biof.2038
ER  - 

@article{
author = "Miler, Marko and Živanović, Jasmina and Ajdžanović, Vladimir and Milenkovic, Dragan and Cesar, Thais and Filipović R., Miloš and Milošević, Verica",
year = "2024",
abstract = "Citrus flavanones are recognized as promising bioactives within the concept of healthy aging. Thus, the present study investigated the effects of a nutritionally relevant dose of lemon extract (LE) on liver redox regulation and persulfidation levels in 24-month-old Wistar rats. LE (40 mg/kg b.m.) was administered orally once daily for four weeks. Control groups received either vehicle (sunflower oil) or remained intact. The applied methodology considered qPCR, Western blot, protein persulfidation levels evaluation, histochemistry in line with immunofluorescence, liver biochemical assays (glutathione, total -SH groups and malonaldehyde; MDA), liver enzymes in serum and in silico analysis to explore the potential interaction/binding between the proteins studied in the paper. Our results showed that LE increased glutathione peroxidase (GPx), reductase (GR), glutamate-cysteine ligase catalytic and modifier subunit, respectively, as well as Nrf2 gene expressions, but decreased the expression of superoxide dismutase 2 (SOD2). Upon LE application, protein expression showed upregulation of NRF2, SOD2, GPx, GR, and thioredoxin 1 (Trx1). LE significantly decreased the protein persulfidation levels and concentration of MDA, a marker of oxidative damage in the cell. Histological analysis showed a normal liver histoarchitecture without pathological changes, aligning with the normal serum level of hepatic enzymes. Obtained results showed that LE, by modulating hepatic redox regulators Nrf2 and Trx1, diminishes oxidative stress and alters the persulfidation levels, suggesting a considerable beneficial antioxidant potential of lemon flavanones in the old-aged liver.",
publisher = "John Wiley and Sons",
journal = "Biofactors",
title = "Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats",
doi = "10.1002/biof.2038"
}

Miler, M., Živanović, J., Ajdžanović, V., Milenkovic, D., Cesar, T., Filipović R., M.,& Milošević, V.. (2024). Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats. in Biofactors
John Wiley and Sons..
https://doi.org/10.1002/biof.2038

Miler M, Živanović J, Ajdžanović V, Milenkovic D, Cesar T, Filipović R. M, Milošević V. Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats. in Biofactors. 2024;.
doi:10.1002/biof.2038 .

Miler, Marko, Živanović, Jasmina, Ajdžanović, Vladimir, Milenkovic, Dragan, Cesar, Thais, Filipović R., Miloš, Milošević, Verica, "Lemon extract reduces the hepatic oxidative stress and persulfidation levels by upregulating the Nrf2 and Trx1 expression in old rats" in Biofactors (2024),
https://doi.org/10.1002/biof.2038 . .