TY  - JOUR
AU  - Vujičić, Milica
AU  - Nikolić, Ivana
AU  - Kontogianni, Vassiliki G.
AU  - Saksida, Tamara
AU  - Charisiadis, Pantelis
AU  - Vasić, Bobana
AU  - Stošić-Grujičić, Stanislava
AU  - Gerothanassis, Ioannis P.
AU  - Tzakos, Andreas G.
AU  - Stojanović, Ivana D.
PY  - 2016
UR  - http://doi.wiley.com/10.1111/1750-3841.13333
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/2979
AB  - Type 1 diabetes (T1D) is an autoimmune disease that develops as a consequence of pancreatic β-cell death induced by proinflammatory mediators. Because Origanum vulgare L. ssp. hirtum (Greek oregano) contains antiinflammatory molecules, we hypothesized that it might be beneficial for the treatment of T1D. An ethyl acetate extract of oregano (EAO) was prepared from the leaves by a polar extraction method. Phytochemical composition was determined by liquid chromatography-UV diode array coupled to ion-trap mass spectrometry with electrospray ionization interface (LC/DAD/ESI-MSn). In vitro immunomodulatory effect of EAO was estimated by measuring proliferation (MTT) or cytokine secretion (ELISA) from immune cells. Diabetes was induced by multiple low doses of streptozotocin (MLDS) in male C57BL/6 mice and EAO was administered intraperitoneally for 10 d. Determination of cellular composition (flow cytometry) and cytokine production (ELISA) was performed on 12th d after diabetes induction. EAO suppressed the function of both macrophages and lymphocytes in vitro. In vivo, EAO treatment significantly preserved pancreatic islets and reduced diabetes incidence in MLDS-challenged mice. Besides down-modulatory effect on macrophages, EAO reduced the number of total CD4+ and activated CD4+CD25+ T cells. Furthermore, EAO affected the number of T helper 1 (Th1) and T helper 17 (Th17) cells through downregulation of their key transcription factors T-bet and RORγT. Because EAO treatment protects mice from development of hyperglycemia by reducing proinflammatory macrophage/Th1/Th17 response, this plant extract could represent a basis for future diabetes therapy.
PB  - Blackwell Publishing Inc.
T2  - Journal of Food Science
T1  - Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin-Induced Diabetes in C57BL/6 Mice
IS  - 7
VL  - 81
DO  - 10.1111/1750-3841.13333
SP  - H1846
EP  - H1853
ER  - 

@article{
author = "Vujičić, Milica and Nikolić, Ivana and Kontogianni, Vassiliki G. and Saksida, Tamara and Charisiadis, Pantelis and Vasić, Bobana and Stošić-Grujičić, Stanislava and Gerothanassis, Ioannis P. and Tzakos, Andreas G. and Stojanović, Ivana D.",
year = "2016",
abstract = "Type 1 diabetes (T1D) is an autoimmune disease that develops as a consequence of pancreatic β-cell death induced by proinflammatory mediators. Because Origanum vulgare L. ssp. hirtum (Greek oregano) contains antiinflammatory molecules, we hypothesized that it might be beneficial for the treatment of T1D. An ethyl acetate extract of oregano (EAO) was prepared from the leaves by a polar extraction method. Phytochemical composition was determined by liquid chromatography-UV diode array coupled to ion-trap mass spectrometry with electrospray ionization interface (LC/DAD/ESI-MSn). In vitro immunomodulatory effect of EAO was estimated by measuring proliferation (MTT) or cytokine secretion (ELISA) from immune cells. Diabetes was induced by multiple low doses of streptozotocin (MLDS) in male C57BL/6 mice and EAO was administered intraperitoneally for 10 d. Determination of cellular composition (flow cytometry) and cytokine production (ELISA) was performed on 12th d after diabetes induction. EAO suppressed the function of both macrophages and lymphocytes in vitro. In vivo, EAO treatment significantly preserved pancreatic islets and reduced diabetes incidence in MLDS-challenged mice. Besides down-modulatory effect on macrophages, EAO reduced the number of total CD4+ and activated CD4+CD25+ T cells. Furthermore, EAO affected the number of T helper 1 (Th1) and T helper 17 (Th17) cells through downregulation of their key transcription factors T-bet and RORγT. Because EAO treatment protects mice from development of hyperglycemia by reducing proinflammatory macrophage/Th1/Th17 response, this plant extract could represent a basis for future diabetes therapy.",
publisher = "Blackwell Publishing Inc.",
journal = "Journal of Food Science",
title = "Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin-Induced Diabetes in C57BL/6 Mice",
number = "7",
volume = "81",
doi = "10.1111/1750-3841.13333",
pages = "H1846-H1853"
}

Vujičić, M., Nikolić, I., Kontogianni, V. G., Saksida, T., Charisiadis, P., Vasić, B., Stošić-Grujičić, S., Gerothanassis, I. P., Tzakos, A. G.,& Stojanović, I. D.. (2016). Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin-Induced Diabetes in C57BL/6 Mice. in Journal of Food Science
Blackwell Publishing Inc.., 81(7), H1846-H1853.
https://doi.org/10.1111/1750-3841.13333

Vujičić M, Nikolić I, Kontogianni VG, Saksida T, Charisiadis P, Vasić B, Stošić-Grujičić S, Gerothanassis IP, Tzakos AG, Stojanović ID. Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin-Induced Diabetes in C57BL/6 Mice. in Journal of Food Science. 2016;81(7):H1846-H1853.
doi:10.1111/1750-3841.13333 .

Vujičić, Milica, Nikolić, Ivana, Kontogianni, Vassiliki G., Saksida, Tamara, Charisiadis, Pantelis, Vasić, Bobana, Stošić-Grujičić, Stanislava, Gerothanassis, Ioannis P., Tzakos, Andreas G., Stojanović, Ivana D., "Ethyl Acetate Extract of Origanum vulgare L. ssp. hirtum Prevents Streptozotocin-Induced Diabetes in C57BL/6 Mice" in Journal of Food Science, 81, no. 7 (2016):H1846-H1853,
https://doi.org/10.1111/1750-3841.13333 . .

TY  - JOUR
AU  - Vujičić, Milica
AU  - Nikolić, Ivana
AU  - Kontogianni, Vassiliki G.
AU  - Saksida, Tamara
AU  - Charisiadis, Pantelis
AU  - Oreščanin Dušić, Zorana
AU  - Blagojević, Duško
AU  - Stošić-Grujičić, Stanislava
AU  - Tzakos, Andreas G.
AU  - Stojanović, Ivana D.
PY  - 2015
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1988
AB  - Type 1 diabetes (T1D), an autoimmune inflammatory disorder, develops as
   a consequence of pancreatic beta-cell destruction and results in
   hyperglycaemia. Since current T1D therapy mainly involves insulin
   replacement, the aim of the present study was to evaluate the
   therapeutic potential of Origanum vulgare L. ssp. hirtum (Greek oregano)
   leaf extract rich in biophenols for the treatment of T1D. The
   phytochemical profile of methanolic oregano extract (MOE) and aqueous
   oregano extract (AOE) was determined by liquid
   chromatography/electrospray ion-trap tandem MS (LC/DAD/ESI-MSn), while
   their main compounds were quantified by HPLC with diode array detection.
   After establishing their potent in vitro antioxidant activity, the
   extracts were administered to C57BL/6 mice treated with multiple low
   doses of streptozotocin for diabetes induction. While prophylactic AOE
   therapy had no impact on diabetes induction, MOE reduced diabetes
   incidence and preserved normal insulin secretion. In addition, MOE
   scavenged reactive oxygen and nitrogen species and, therefore,
   alleviated the need for the up-regulation of antioxidant enzymes. MOE
   treatment specifically attenuated the pro-inflammatory response mediated
   by T helper 17 cells and enhanced anti-inflammatory T helper 2 and T
   regulatory cells through the impact on specific signalling pathways and
   transcription factors. Importantly, MOE preserved beta-cells from in
   vitro apoptosis via blockade of caspase 3. Finally, rosmarinic acid, a
   predominant compound in MOE, exhibited only partial protection from
   diabetes induction. In conclusion, acting as an antioxidant,
   immunomodulator and in an anti-apoptotic manner, MOE protected mice from
   diabetes development. Seemingly, there is more than one compound
   responsible for the beneficial effect of MOE.
T2  - British Journal of Nutrition
T1  - Methanolic extract of Origanum vulgare ameliorates type 1 diabetes
 through antioxidant, anti-inflammatory and anti-apoptotic activity
IS  - 5
VL  - 113
DO  - 10.1017/S0007114514004048
SP  - 770
EP  - 782
ER  - 

@article{
author = "Vujičić, Milica and Nikolić, Ivana and Kontogianni, Vassiliki G. and Saksida, Tamara and Charisiadis, Pantelis and Oreščanin Dušić, Zorana and Blagojević, Duško and Stošić-Grujičić, Stanislava and Tzakos, Andreas G. and Stojanović, Ivana D.",
year = "2015",
abstract = "Type 1 diabetes (T1D), an autoimmune inflammatory disorder, develops as
   a consequence of pancreatic beta-cell destruction and results in
   hyperglycaemia. Since current T1D therapy mainly involves insulin
   replacement, the aim of the present study was to evaluate the
   therapeutic potential of Origanum vulgare L. ssp. hirtum (Greek oregano)
   leaf extract rich in biophenols for the treatment of T1D. The
   phytochemical profile of methanolic oregano extract (MOE) and aqueous
   oregano extract (AOE) was determined by liquid
   chromatography/electrospray ion-trap tandem MS (LC/DAD/ESI-MSn), while
   their main compounds were quantified by HPLC with diode array detection.
   After establishing their potent in vitro antioxidant activity, the
   extracts were administered to C57BL/6 mice treated with multiple low
   doses of streptozotocin for diabetes induction. While prophylactic AOE
   therapy had no impact on diabetes induction, MOE reduced diabetes
   incidence and preserved normal insulin secretion. In addition, MOE
   scavenged reactive oxygen and nitrogen species and, therefore,
   alleviated the need for the up-regulation of antioxidant enzymes. MOE
   treatment specifically attenuated the pro-inflammatory response mediated
   by T helper 17 cells and enhanced anti-inflammatory T helper 2 and T
   regulatory cells through the impact on specific signalling pathways and
   transcription factors. Importantly, MOE preserved beta-cells from in
   vitro apoptosis via blockade of caspase 3. Finally, rosmarinic acid, a
   predominant compound in MOE, exhibited only partial protection from
   diabetes induction. In conclusion, acting as an antioxidant,
   immunomodulator and in an anti-apoptotic manner, MOE protected mice from
   diabetes development. Seemingly, there is more than one compound
   responsible for the beneficial effect of MOE.",
journal = "British Journal of Nutrition",
title = "Methanolic extract of Origanum vulgare ameliorates type 1 diabetes
 through antioxidant, anti-inflammatory and anti-apoptotic activity",
number = "5",
volume = "113",
doi = "10.1017/S0007114514004048",
pages = "770-782"
}

Vujičić, M., Nikolić, I., Kontogianni, V. G., Saksida, T., Charisiadis, P., Oreščanin Dušić, Z., Blagojević, D., Stošić-Grujičić, S., Tzakos, A. G.,& Stojanović, I. D.. (2015). Methanolic extract of Origanum vulgare ameliorates type 1 diabetes
 through antioxidant, anti-inflammatory and anti-apoptotic activity. in British Journal of Nutrition, 113(5), 770-782.
https://doi.org/10.1017/S0007114514004048

Vujičić M, Nikolić I, Kontogianni VG, Saksida T, Charisiadis P, Oreščanin Dušić Z, Blagojević D, Stošić-Grujičić S, Tzakos AG, Stojanović ID. Methanolic extract of Origanum vulgare ameliorates type 1 diabetes
 through antioxidant, anti-inflammatory and anti-apoptotic activity. in British Journal of Nutrition. 2015;113(5):770-782.
doi:10.1017/S0007114514004048 .

Vujičić, Milica, Nikolić, Ivana, Kontogianni, Vassiliki G., Saksida, Tamara, Charisiadis, Pantelis, Oreščanin Dušić, Zorana, Blagojević, Duško, Stošić-Grujičić, Stanislava, Tzakos, Andreas G., Stojanović, Ivana D., "Methanolic extract of Origanum vulgare ameliorates type 1 diabetes
 through antioxidant, anti-inflammatory and anti-apoptotic activity" in British Journal of Nutrition, 113, no. 5 (2015):770-782,
https://doi.org/10.1017/S0007114514004048 . .