TY  - JOUR
AU  - Ćalić-Dragosavac, Dušica D.
AU  - Radojević, Ljiljana R.
AU  - Zdravković-Korać, Snežana
AU  - Savikin-Fodulović, Katarina P.
AU  - Vinterhalter, Branka
PY  - 2010
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1382
AB  - Escin, a group of chemically related triterpenic glycosides, is widely used in commercial preparations for the treatment of venous insufficiency. Since the zygotic embryo cotyledons accumulate the highest amount of escin, it is currently extracted from the seeds of horse chestnut, Aesculus hippocastanum L. (Hippocastanaceae), on a large scale. As this material is available during only short period of the year, we studied the possibility of using plant tissue culture to obtain escin. For this purpose, the content of escin in androgenic embryos and hairy root cultures of horse chestnut was studied. Escin content was found to be dependent on the stage of androgenic embryo development and the type of phytoregulator supplemented to the nutritive medium. In the absence of phytoregulators, androgenic embryos at the globular stage of development contained approximately four times less escin than those at the cotyledonary stage. Inclusion of various phytoregulators in the nutritive media stimulated escin production. Among them, 2,4-dichlorophenoxyacetic acid (2,4-D) showed the most pronounced effect, with escin content almost reaching that found in zygotic embryos (6.77% versus 6.96%). Two hairy root clones produced substantial amounts of escin (3.57% and 4.09%), less than zygotic embryos, but higher than cotyledonary embryos on phytoregulator-free medium.</.
T2  - Pharmaceutical Biology
T1  - Determination of escin content in androgenic embryos and hairy root culture of Aesculus hippocastanum
IS  - 5
VL  - 48
EP  - 567
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1382
ER  - 

@article{
author = "Ćalić-Dragosavac, Dušica D. and Radojević, Ljiljana R. and Zdravković-Korać, Snežana and Savikin-Fodulović, Katarina P. and Vinterhalter, Branka",
year = "2010",
abstract = "Escin, a group of chemically related triterpenic glycosides, is widely used in commercial preparations for the treatment of venous insufficiency. Since the zygotic embryo cotyledons accumulate the highest amount of escin, it is currently extracted from the seeds of horse chestnut, Aesculus hippocastanum L. (Hippocastanaceae), on a large scale. As this material is available during only short period of the year, we studied the possibility of using plant tissue culture to obtain escin. For this purpose, the content of escin in androgenic embryos and hairy root cultures of horse chestnut was studied. Escin content was found to be dependent on the stage of androgenic embryo development and the type of phytoregulator supplemented to the nutritive medium. In the absence of phytoregulators, androgenic embryos at the globular stage of development contained approximately four times less escin than those at the cotyledonary stage. Inclusion of various phytoregulators in the nutritive media stimulated escin production. Among them, 2,4-dichlorophenoxyacetic acid (2,4-D) showed the most pronounced effect, with escin content almost reaching that found in zygotic embryos (6.77% versus 6.96%). Two hairy root clones produced substantial amounts of escin (3.57% and 4.09%), less than zygotic embryos, but higher than cotyledonary embryos on phytoregulator-free medium.</.",
journal = "Pharmaceutical Biology",
title = "Determination of escin content in androgenic embryos and hairy root culture of Aesculus hippocastanum",
number = "5",
volume = "48",
pages = "567",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1382"
}

Ćalić-Dragosavac, D. D., Radojević, L. R., Zdravković-Korać, S., Savikin-Fodulović, K. P.,& Vinterhalter, B.. (2010). Determination of escin content in androgenic embryos and hairy root culture of Aesculus hippocastanum. in Pharmaceutical Biology, 48(5).
https://hdl.handle.net/21.15107/rcub_ibiss_1382

Ćalić-Dragosavac DD, Radojević LR, Zdravković-Korać S, Savikin-Fodulović KP, Vinterhalter B. Determination of escin content in androgenic embryos and hairy root culture of Aesculus hippocastanum. in Pharmaceutical Biology. 2010;48(5):null-567.
https://hdl.handle.net/21.15107/rcub_ibiss_1382 .

Ćalić-Dragosavac, Dušica D., Radojević, Ljiljana R., Zdravković-Korać, Snežana, Savikin-Fodulović, Katarina P., Vinterhalter, Branka, "Determination of escin content in androgenic embryos and hairy root culture of Aesculus hippocastanum" in Pharmaceutical Biology, 48, no. 5 (2010),
https://hdl.handle.net/21.15107/rcub_ibiss_1382 .

TY  - JOUR
AU  - Janković, Teodora
AU  - Krstić, Dijana
AU  - Aljancić, Ivana S
AU  - Savikin-Fodulović, Katarina P.
AU  - Menković, Nebojsa R
AU  - Vajs, Vlatka E
AU  - Milosavljević, Slobodan M
PY  - 2005
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1698
T2  - Biochemical Systematics and Ecology
T1  - Xanthones and C-glucosides from the aerial parts of four species of Gentianella from Serbia and Montenegro
IS  - 7
VL  - 33
EP  - 735
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1698
ER  - 

@article{
author = "Janković, Teodora and Krstić, Dijana and Aljancić, Ivana S and Savikin-Fodulović, Katarina P. and Menković, Nebojsa R and Vajs, Vlatka E and Milosavljević, Slobodan M",
year = "2005",
journal = "Biochemical Systematics and Ecology",
title = "Xanthones and C-glucosides from the aerial parts of four species of Gentianella from Serbia and Montenegro",
number = "7",
volume = "33",
pages = "735",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1698"
}

Janković, T., Krstić, D., Aljancić, I. S., Savikin-Fodulović, K. P., Menković, N. R., Vajs, V. E.,& Milosavljević, S. M.. (2005). Xanthones and C-glucosides from the aerial parts of four species of Gentianella from Serbia and Montenegro. in Biochemical Systematics and Ecology, 33(7).
https://hdl.handle.net/21.15107/rcub_ibiss_1698

Janković T, Krstić D, Aljancić IS, Savikin-Fodulović KP, Menković NR, Vajs VE, Milosavljević SM. Xanthones and C-glucosides from the aerial parts of four species of Gentianella from Serbia and Montenegro. in Biochemical Systematics and Ecology. 2005;33(7):null-735.
https://hdl.handle.net/21.15107/rcub_ibiss_1698 .

Janković, Teodora, Krstić, Dijana, Aljancić, Ivana S, Savikin-Fodulović, Katarina P., Menković, Nebojsa R, Vajs, Vlatka E, Milosavljević, Slobodan M, "Xanthones and C-glucosides from the aerial parts of four species of Gentianella from Serbia and Montenegro" in Biochemical Systematics and Ecology, 33, no. 7 (2005),
https://hdl.handle.net/21.15107/rcub_ibiss_1698 .

TY  - JOUR
AU  - Krstić, Dijana
AU  - Janković, Teodora
AU  - Aljancić, Ivana S
AU  - Savikin-Fodulović, Katarina P.
AU  - Menković, Nebojsa R
AU  - Milosavljević, Slobodan M
PY  - 2004
UR  - https://radar.ibiss.bg.ac.rs/handle/123456789/1724
T2  - Biochemical Systematics and Ecology
T1  - Phytochemical investigation of Gentiana dinarica
IS  - 10
VL  - 32
EP  - 941
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1724
ER  - 

@article{
author = "Krstić, Dijana and Janković, Teodora and Aljancić, Ivana S and Savikin-Fodulović, Katarina P. and Menković, Nebojsa R and Milosavljević, Slobodan M",
year = "2004",
journal = "Biochemical Systematics and Ecology",
title = "Phytochemical investigation of Gentiana dinarica",
number = "10",
volume = "32",
pages = "941",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1724"
}

Krstić, D., Janković, T., Aljancić, I. S., Savikin-Fodulović, K. P., Menković, N. R.,& Milosavljević, S. M.. (2004). Phytochemical investigation of Gentiana dinarica. in Biochemical Systematics and Ecology, 32(10).
https://hdl.handle.net/21.15107/rcub_ibiss_1724

Krstić D, Janković T, Aljancić IS, Savikin-Fodulović KP, Menković NR, Milosavljević SM. Phytochemical investigation of Gentiana dinarica. in Biochemical Systematics and Ecology. 2004;32(10):null-941.
https://hdl.handle.net/21.15107/rcub_ibiss_1724 .

Krstić, Dijana, Janković, Teodora, Aljancić, Ivana S, Savikin-Fodulović, Katarina P., Menković, Nebojsa R, Milosavljević, Slobodan M, "Phytochemical investigation of Gentiana dinarica" in Biochemical Systematics and Ecology, 32, no. 10 (2004),
https://hdl.handle.net/21.15107/rcub_ibiss_1724 .